In order to assess the responses of different skin types stimulated by tape stripping,after which two kinds of brand cosmetics of cream and hydrogel,were separately applied to evaluate the individual soothing effect o...In order to assess the responses of different skin types stimulated by tape stripping,after which two kinds of brand cosmetics of cream and hydrogel,were separately applied to evaluate the individual soothing effect of the product on different skin types.A total of 30 volunteers aged 18~65 years were recruited,which were divided into four groups(oily,dry,normal and combined)according to the self-oiling ability of facial and T zones.Four 3 cm×3 cm of skins on inner forearm of each volunteer were chosen and randomly marked as blank,negative control,soothing cream and soothing gel.The blank area was kept untreated,and the remaining areas were tape-stripped by 10 times(Transparent universal 1527C-1,3M China Co.,Ltd.,)to disrupt the skin corneum and induce erythema.Images,erythema index(EI)and transcutaneous water loss rate(TEWL)of the marked skin areas before and 5 min after tape-stripping were taken to assess the distinct responses of different skin type to the taping treatment;Ten minutes after tape-stripping,The soothing products were applied to the test area,the skin images,EI and TEWL at 20 min and 50 min(corresponding to 30 min and 60 min after tape-stripping,respectively)were measured after cosmetics application to compare and evaluate the soothing effects of cream and hydrogel on different skin types.Results showed that different skin displayed diverse reactions to the tape-stripping,and the oily skin was more sensitive with longer duration of erythema than other skin types.After the application of soothing products,both cream and hydrogel could relieve skin erythema,while the soothing effects are dependent both on skin types and the cosmetic base materials:Hydrogel has a better efficacy in alleviating erythema and TEWL of dry skin,while the cream was more effective on normal,oily and combined skin types.The results suggest that the modelling method for soothing evaluation should consider the skin types of the volunteers,and the cosmetics of different base materials might have the target skin types.展开更多
To explore the method of evaluating the soothing effect of human skin damage,a human skin damage model was established using UV light induction.Four test areas were set up,namely blank control area,UV damage preventio...To explore the method of evaluating the soothing effect of human skin damage,a human skin damage model was established using UV light induction.Four test areas were set up,namely blank control area,UV damage prevention and soothing area,immediate soothing area after UV damage and soothing area after UV damage.Five skin parameters,including skin melanin,red pigment value,skin pigmentation value,a*value,and skin redness value,were used to characterize skin pigmentation before and after using the sample Changes in properties such as skin erythema and skin pigment.The results showed that the method showed significant changes in the skin condition of volunteers before and after using the sample,and could achieve a soothing effect,which has certain reference significance.展开更多
Present study is aimed to investigate in vitro biological efficacy of Tambourissa trichophylla leaf extract(TTLE),a plant-derived ingredient in cosmetic,on antioxidant activity and soothing efficacy.The total phenols ...Present study is aimed to investigate in vitro biological efficacy of Tambourissa trichophylla leaf extract(TTLE),a plant-derived ingredient in cosmetic,on antioxidant activity and soothing efficacy.The total phenols content of TTLE was determined by the Folin-Ciocalteu method,antioxidant activity was tested by DPPH free radical scavenging assay,hyaluronidase inhibition activity was performed by using in vitro enzyme inhibitory assays based on spectrophotometric evaluation,and the soothing efficacy of TTLE was performed by the expression of nitric oxide(NO)and interleukin-6(IL-6)produced by LPS-induced RAW264.7 cells.The results shown that the total phenolic content of TTLE was as high as 54%,the DPPH scavenging activity was 81.18%at the concentration of 25μg/mL,the hyaluronidase inhibition activity was 69.04%at the concentration of 5 mg/mL,and the inhibition of NO and IL-6 were 15.70%and 76.57%at 0.05 mg/mL,respectively.TTLE has a high content of total phenols,high antioxidant activity and soothing effects,which indicated that TTLE was a promising ingredient in the development and application of cosmetics.展开更多
In order to search an appropriate evaluation method for the soothing effect of cosmetics,the LPS-induced macrophage model,SDS-stimulated zebrafish embryos model and the clinical assessment were used.The results showed...In order to search an appropriate evaluation method for the soothing effect of cosmetics,the LPS-induced macrophage model,SDS-stimulated zebrafish embryos model and the clinical assessment were used.The results showed that the concentration of NO and IL-6 was significantly reduced in LPS-induced RAW264.7 cells when applied to two essences.The inhibition rate of NO-release was 85.87%and 84.57%,while IL-6 was 44.18%and 38.76%,respectively.Furthermore,the spontaneous rotating of zebrafish embryos was significantly decreased to 51.85%and 76.54%in SDS-stimulated zebrafish embryos model.In the 28 days of clinical assessment,the concentration of hydration was significantly increased to 55.56%and 39.61%,while the erythrodesis was significantly decreased to 4.75%and 6.68%.This indicates that the three evaluation methods of soothing efficacy in this research were effective,and can be used in the evaluation of cosmetics.展开更多
p-Hydroxyacetophenone(p-HAP)was used as preservatives in cosmetics recently,their new efficacy was rarely reported.Here,We evaluate the soothing and oil control efficacy of p-HAP in vitro cells,providing a new efficac...p-Hydroxyacetophenone(p-HAP)was used as preservatives in cosmetics recently,their new efficacy was rarely reported.Here,We evaluate the soothing and oil control efficacy of p-HAP in vitro cells,providing a new efficacy basis for its application in cosmetics.The content of nitric oxide(NO),tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)were determined in LPS-induced RAW264.7 and lipid contents were determined in testosterone-induced SZ95.The results revealed that p-HAP attenuated pro-inflammatory cytokines,such as NO,TNF-αand IL-6(P<0.01).Simultaneously,0.05mg/mL of p-HAP also inhibited the formation of lipid droplets in human sebocytes,with 17.64%of inhibition(P<0.001).These findings may be beneficial in the application of p-HAP as an efficacy ingredient in cosmetics,which may have a soothing and oil control efficacy according to the inhibition of pro-inflammatory cytokines and lipid contents.展开更多
The skin is a formidable physical and biological barrier which communicates continuously with the outside of the body. And the stratum corneum, the outermost layer of human epidermis, plays a central role in the inter...The skin is a formidable physical and biological barrier which communicates continuously with the outside of the body. And the stratum corneum, the outermost layer of human epidermis, plays a central role in the interaction between the cutaneous tissue and the external environment. The horny layer, and more generally the whole skin layers, avoid the penetration of harmful exogenous agents, produce molecules named anti-microbial peptides which impact the composition of the cutaneous microbiota, regulate the internal corporal temperature, avoid the water loss from the inside of the body and constitute an incredible efficient anti-oxidant network. Nevertheless, nowadays, the skin is more and more solicited by the different elements of the cutaneous exposome, including atmospheric pollution and solar radiations, which can cause a dramatic acceleration of the skin ageing process. As a consequence, due to the multifunctional protective role of the skin, during the recent decade the cosmetic industry invested massively in the development of new raw materials and end-products (dermo-cosmetics) able to preserve an optimal state of the skin regarding the external environment. Based on their physical-chemical properties thermal spring waters, which are extremely rich in inorganics ions, are interesting and powerful candidates to be part, as integral component, of new efficient dermo-cosmetic formulations dedicated to protect the skin from the external stimuli. The aim of the present work was to investigate and characterize the activity of Jonzac thermal spring water on the skin. Using different models, we proved for the first time that Jonzac thermal spring water reinforces the barrier function of the skin by modulating the expression of key markers including filaggrin and human beta defensin 2 on ex vivo human skin. The ex vivo and in vivo hydration activity, by Raman spectroscopy and corneometry respectively, has been also demonstrated. We have also shown that Jonzac thermal spring water ameliorates significantly the cutaneous microrelief in vivo. To conclude, we characterize the soothing effect of Jonzac thermal spring water by the analysis of histamine release in Substance P treated skin explants and by measuring the redness of the skin following UV exposure of the skin in vivo. We observed that both parameters decreased following a preventive treatment of the skin with Jonzac thermal spring water. Taken together our results indicate that Jonzac thermal spring water is a promising and powerful dermo-cosmetic which can be used to preserve an optimal state of the cutaneous tissue.展开更多
文摘In order to assess the responses of different skin types stimulated by tape stripping,after which two kinds of brand cosmetics of cream and hydrogel,were separately applied to evaluate the individual soothing effect of the product on different skin types.A total of 30 volunteers aged 18~65 years were recruited,which were divided into four groups(oily,dry,normal and combined)according to the self-oiling ability of facial and T zones.Four 3 cm×3 cm of skins on inner forearm of each volunteer were chosen and randomly marked as blank,negative control,soothing cream and soothing gel.The blank area was kept untreated,and the remaining areas were tape-stripped by 10 times(Transparent universal 1527C-1,3M China Co.,Ltd.,)to disrupt the skin corneum and induce erythema.Images,erythema index(EI)and transcutaneous water loss rate(TEWL)of the marked skin areas before and 5 min after tape-stripping were taken to assess the distinct responses of different skin type to the taping treatment;Ten minutes after tape-stripping,The soothing products were applied to the test area,the skin images,EI and TEWL at 20 min and 50 min(corresponding to 30 min and 60 min after tape-stripping,respectively)were measured after cosmetics application to compare and evaluate the soothing effects of cream and hydrogel on different skin types.Results showed that different skin displayed diverse reactions to the tape-stripping,and the oily skin was more sensitive with longer duration of erythema than other skin types.After the application of soothing products,both cream and hydrogel could relieve skin erythema,while the soothing effects are dependent both on skin types and the cosmetic base materials:Hydrogel has a better efficacy in alleviating erythema and TEWL of dry skin,while the cream was more effective on normal,oily and combined skin types.The results suggest that the modelling method for soothing evaluation should consider the skin types of the volunteers,and the cosmetics of different base materials might have the target skin types.
文摘To explore the method of evaluating the soothing effect of human skin damage,a human skin damage model was established using UV light induction.Four test areas were set up,namely blank control area,UV damage prevention and soothing area,immediate soothing area after UV damage and soothing area after UV damage.Five skin parameters,including skin melanin,red pigment value,skin pigmentation value,a*value,and skin redness value,were used to characterize skin pigmentation before and after using the sample Changes in properties such as skin erythema and skin pigment.The results showed that the method showed significant changes in the skin condition of volunteers before and after using the sample,and could achieve a soothing effect,which has certain reference significance.
文摘Present study is aimed to investigate in vitro biological efficacy of Tambourissa trichophylla leaf extract(TTLE),a plant-derived ingredient in cosmetic,on antioxidant activity and soothing efficacy.The total phenols content of TTLE was determined by the Folin-Ciocalteu method,antioxidant activity was tested by DPPH free radical scavenging assay,hyaluronidase inhibition activity was performed by using in vitro enzyme inhibitory assays based on spectrophotometric evaluation,and the soothing efficacy of TTLE was performed by the expression of nitric oxide(NO)and interleukin-6(IL-6)produced by LPS-induced RAW264.7 cells.The results shown that the total phenolic content of TTLE was as high as 54%,the DPPH scavenging activity was 81.18%at the concentration of 25μg/mL,the hyaluronidase inhibition activity was 69.04%at the concentration of 5 mg/mL,and the inhibition of NO and IL-6 were 15.70%and 76.57%at 0.05 mg/mL,respectively.TTLE has a high content of total phenols,high antioxidant activity and soothing effects,which indicated that TTLE was a promising ingredient in the development and application of cosmetics.
文摘In order to search an appropriate evaluation method for the soothing effect of cosmetics,the LPS-induced macrophage model,SDS-stimulated zebrafish embryos model and the clinical assessment were used.The results showed that the concentration of NO and IL-6 was significantly reduced in LPS-induced RAW264.7 cells when applied to two essences.The inhibition rate of NO-release was 85.87%and 84.57%,while IL-6 was 44.18%and 38.76%,respectively.Furthermore,the spontaneous rotating of zebrafish embryos was significantly decreased to 51.85%and 76.54%in SDS-stimulated zebrafish embryos model.In the 28 days of clinical assessment,the concentration of hydration was significantly increased to 55.56%and 39.61%,while the erythrodesis was significantly decreased to 4.75%and 6.68%.This indicates that the three evaluation methods of soothing efficacy in this research were effective,and can be used in the evaluation of cosmetics.
文摘p-Hydroxyacetophenone(p-HAP)was used as preservatives in cosmetics recently,their new efficacy was rarely reported.Here,We evaluate the soothing and oil control efficacy of p-HAP in vitro cells,providing a new efficacy basis for its application in cosmetics.The content of nitric oxide(NO),tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)were determined in LPS-induced RAW264.7 and lipid contents were determined in testosterone-induced SZ95.The results revealed that p-HAP attenuated pro-inflammatory cytokines,such as NO,TNF-αand IL-6(P<0.01).Simultaneously,0.05mg/mL of p-HAP also inhibited the formation of lipid droplets in human sebocytes,with 17.64%of inhibition(P<0.001).These findings may be beneficial in the application of p-HAP as an efficacy ingredient in cosmetics,which may have a soothing and oil control efficacy according to the inhibition of pro-inflammatory cytokines and lipid contents.
文摘The skin is a formidable physical and biological barrier which communicates continuously with the outside of the body. And the stratum corneum, the outermost layer of human epidermis, plays a central role in the interaction between the cutaneous tissue and the external environment. The horny layer, and more generally the whole skin layers, avoid the penetration of harmful exogenous agents, produce molecules named anti-microbial peptides which impact the composition of the cutaneous microbiota, regulate the internal corporal temperature, avoid the water loss from the inside of the body and constitute an incredible efficient anti-oxidant network. Nevertheless, nowadays, the skin is more and more solicited by the different elements of the cutaneous exposome, including atmospheric pollution and solar radiations, which can cause a dramatic acceleration of the skin ageing process. As a consequence, due to the multifunctional protective role of the skin, during the recent decade the cosmetic industry invested massively in the development of new raw materials and end-products (dermo-cosmetics) able to preserve an optimal state of the skin regarding the external environment. Based on their physical-chemical properties thermal spring waters, which are extremely rich in inorganics ions, are interesting and powerful candidates to be part, as integral component, of new efficient dermo-cosmetic formulations dedicated to protect the skin from the external stimuli. The aim of the present work was to investigate and characterize the activity of Jonzac thermal spring water on the skin. Using different models, we proved for the first time that Jonzac thermal spring water reinforces the barrier function of the skin by modulating the expression of key markers including filaggrin and human beta defensin 2 on ex vivo human skin. The ex vivo and in vivo hydration activity, by Raman spectroscopy and corneometry respectively, has been also demonstrated. We have also shown that Jonzac thermal spring water ameliorates significantly the cutaneous microrelief in vivo. To conclude, we characterize the soothing effect of Jonzac thermal spring water by the analysis of histamine release in Substance P treated skin explants and by measuring the redness of the skin following UV exposure of the skin in vivo. We observed that both parameters decreased following a preventive treatment of the skin with Jonzac thermal spring water. Taken together our results indicate that Jonzac thermal spring water is a promising and powerful dermo-cosmetic which can be used to preserve an optimal state of the cutaneous tissue.
