This study was conducted to identify resistance sources against the newly documented pathotypes (P5 and P6) of </span><i><span style="font-family:Verdana;">Sporisorium</span></i>...This study was conducted to identify resistance sources against the newly documented pathotypes (P5 and P6) of </span><i><span style="font-family:Verdana;">Sporisorium</span></i> <i><span style="font-family:Verdana;">reilianum</span></i><span style="font-family:Verdana;">, causing sorghum head smut. A subset of 67 sorghum association panel (SAP) accessions, 29 in 2017 and 38 in 2018 along with checks BTx635 (resistant) and BTx643 (susceptible) were screened in the greenhouse against P5 and P6 pathotypes in two separate experiments in both years. At 18 to 20 days after planting, accessions were inoculated by injecting the seedlings below the apical meristem with sporidial suspensions following an established inoculation procedure. Three accessions (PI656091, PI533919, and PI533821) in 2017 </span><span style="font-family:Verdana;">and 17 accessions (PI597961, PI656071, PI656048, PI576435, PI534075, PI534145,</span><span style="font-family:Verdana;"> PI656057, PI576437, PI651492, PI656014, PI656025, PI655999, PI656010, PI656082, PI534123, PI533927, PI656100) in 2018 were identified as potential resistant sources to both P5 and P6 pathotypes of </span><i><span style="font-family:Verdana;">S.</span></i> <i><span style="font-family:Verdana;">reilianum</span></i><span style="font-family:Verdana;">. These SAP accessions are new sources that can be further used to develop new breeding parental lines and hybrids and mapping populations for quantitative trait loci markers closely linked to head smut resistance.展开更多
为了弄清田间多样的高粱黑穗病症状与病原种类间的关系,将采自我国北方高粱生产田中不同症状的高粱黑穗病菌,通过病菌培养性状与r DNA ITS序列分析,研究病菌种群多样性。结果表明:虽然高粱黑穗病症状差异很大,但病菌冬孢子萌发特点、菌...为了弄清田间多样的高粱黑穗病症状与病原种类间的关系,将采自我国北方高粱生产田中不同症状的高粱黑穗病菌,通过病菌培养性状与r DNA ITS序列分析,研究病菌种群多样性。结果表明:虽然高粱黑穗病症状差异很大,但病菌冬孢子萌发特点、菌落形态特征等无明显差异,且与高粱丝黑穗病的致病菌丝孢堆黑粉菌[Sporisorium reilianum(Kühn)Langdon&Fullerton]的特点相符合;供试菌株r DNA-ITS序列(序列号:KC986979)与Gen Bank中的丝孢堆黑粉菌(S.reilianum)的序列相似度达99%;田间高粱黑穗病的不同症状均由丝黑穗病的致病菌——丝孢堆黑粉菌侵染所致,并非属于其他黑粉菌种类。高粱黑穗病症状差异的原因尚有待于进一步研究。展开更多
文摘This study was conducted to identify resistance sources against the newly documented pathotypes (P5 and P6) of </span><i><span style="font-family:Verdana;">Sporisorium</span></i> <i><span style="font-family:Verdana;">reilianum</span></i><span style="font-family:Verdana;">, causing sorghum head smut. A subset of 67 sorghum association panel (SAP) accessions, 29 in 2017 and 38 in 2018 along with checks BTx635 (resistant) and BTx643 (susceptible) were screened in the greenhouse against P5 and P6 pathotypes in two separate experiments in both years. At 18 to 20 days after planting, accessions were inoculated by injecting the seedlings below the apical meristem with sporidial suspensions following an established inoculation procedure. Three accessions (PI656091, PI533919, and PI533821) in 2017 </span><span style="font-family:Verdana;">and 17 accessions (PI597961, PI656071, PI656048, PI576435, PI534075, PI534145,</span><span style="font-family:Verdana;"> PI656057, PI576437, PI651492, PI656014, PI656025, PI655999, PI656010, PI656082, PI534123, PI533927, PI656100) in 2018 were identified as potential resistant sources to both P5 and P6 pathotypes of </span><i><span style="font-family:Verdana;">S.</span></i> <i><span style="font-family:Verdana;">reilianum</span></i><span style="font-family:Verdana;">. These SAP accessions are new sources that can be further used to develop new breeding parental lines and hybrids and mapping populations for quantitative trait loci markers closely linked to head smut resistance.
