LeSPL-CNR is a crucial transcription factor for fruit ripening of Solanum lycopersicum. The cnr (colorless non-ripening) epimutation resulted from hypermethylation in a 286 bp region of LeSPL-CNR promoter inhibits nor...LeSPL-CNR is a crucial transcription factor for fruit ripening of Solanum lycopersicum. The cnr (colorless non-ripening) epimutation resulted from hypermethylation in a 286 bp region of LeSPL-CNR promoter inhibits normal fruit ripening. In present study, potential regulators of LeSPL-CNR, which could bind to the specific 286 bp region, were screened via south-western blotting and yeast one-hybrid (Y1H) library screening system. Results indicated that a total of 13 and 19 candidate proteins were acquired respectively, and both ribulose-1,5-bisphosphate carboxylase/oxygenase and 40S ribosomal protein were identified by two methods. These would provide some information for revealing roles of DNA methylation and the regulatory mechanism for LeSPL-CNR.展开更多
Radiogenic carbon (14C) of the DIC (Dissolved Inorganic Carbon) and oxygen-18 were used to understand the hydro- dynamic functioning of the multilayer aquifer system in the Moulares mining basin, southern Tunisia. The...Radiogenic carbon (14C) of the DIC (Dissolved Inorganic Carbon) and oxygen-18 were used to understand the hydro- dynamic functioning of the multilayer aquifer system in the Moulares mining basin, southern Tunisia. The results of this study permits identify two groundwater types. A- an old paleoclimatic groundwater, marked by low carbon-14 (14C) activities. B - a recent groundwater, was distinguished by relatively high carbon-14 activities. In addition to these two water types, other groundwater, indicating a mixing effect, is resulting presumably from upward movement from the deeper groundwater. Based on 14C activity and the piston flow type theory the groundwater residence time varies from 5 Kyear to 35 Kyear. Carbon-14 activity and oxygen-18 in Groundwater are active since Mio-Plio-Quaternary and Upper Cretaceous aquifers lead to the identification of paleorecharged water probably during Late Pleistocene and Early to Middle Holocene. The water feedings of these aquifers are mainly provided by infiltration of precipitations, infiltration of irrigation water, lateral feeding from cretaceous relieves from the South and the North and along recent and fossil drainage networks that constitute major fresh water sources in groundwater tables.展开更多
文摘LeSPL-CNR is a crucial transcription factor for fruit ripening of Solanum lycopersicum. The cnr (colorless non-ripening) epimutation resulted from hypermethylation in a 286 bp region of LeSPL-CNR promoter inhibits normal fruit ripening. In present study, potential regulators of LeSPL-CNR, which could bind to the specific 286 bp region, were screened via south-western blotting and yeast one-hybrid (Y1H) library screening system. Results indicated that a total of 13 and 19 candidate proteins were acquired respectively, and both ribulose-1,5-bisphosphate carboxylase/oxygenase and 40S ribosomal protein were identified by two methods. These would provide some information for revealing roles of DNA methylation and the regulatory mechanism for LeSPL-CNR.
文摘Radiogenic carbon (14C) of the DIC (Dissolved Inorganic Carbon) and oxygen-18 were used to understand the hydro- dynamic functioning of the multilayer aquifer system in the Moulares mining basin, southern Tunisia. The results of this study permits identify two groundwater types. A- an old paleoclimatic groundwater, marked by low carbon-14 (14C) activities. B - a recent groundwater, was distinguished by relatively high carbon-14 activities. In addition to these two water types, other groundwater, indicating a mixing effect, is resulting presumably from upward movement from the deeper groundwater. Based on 14C activity and the piston flow type theory the groundwater residence time varies from 5 Kyear to 35 Kyear. Carbon-14 activity and oxygen-18 in Groundwater are active since Mio-Plio-Quaternary and Upper Cretaceous aquifers lead to the identification of paleorecharged water probably during Late Pleistocene and Early to Middle Holocene. The water feedings of these aquifers are mainly provided by infiltration of precipitations, infiltration of irrigation water, lateral feeding from cretaceous relieves from the South and the North and along recent and fossil drainage networks that constitute major fresh water sources in groundwater tables.
