Xanthophyll has multiple physiological functions to improve the quality of farmed animals.The present study aimed to investigate the effects of xanthophyll on the growth performance,antioxidation,immunity,pigmentation...Xanthophyll has multiple physiological functions to improve the quality of farmed animals.The present study aimed to investigate the effects of xanthophyll on the growth performance,antioxidation,immunity,pigmentation and meat quality of southern catfish(Silurus soldatovi meridionalis Chen).Juvenile southern catfish(18.35±0.04 g)were randomly allocated into 24 cages(30 juveniles per cage),and fed diets with different dietary xanthophyll levels(at 14,42,80,108,126 and 152 mg/kg,dry matter of diet)twice daily for 8 weeks.Results indicated that the diet with 80 mg/kg xanthophyll induced a higher specific growth rate(SGR),feed efficiency ratio and protein productive value.Moreover,the 80 mg/kg xanthophyll diet also increased complement 3,immunoglobulin M(IgM)and lysozyme content more than the other groups.The mRNA expression level of inflammation-related genes and antioxidant enzyme activities further confirmed the effects of 80 mg/kg dietary xanthophyll on improving immune response.The present study also found that the 126 mg/kg xanthophyll diet significantly enhanced the content of total carotenoids and xanthophyll,hydroxyproline,collagen and amino acid in muscle.The diet with 126 mg/kg xanthophylls also induced lower drip loss,thawing loss,centrifugal loss,cooking loss and higher muscle adhesiveness,cohesiveness,springiness,gumminess and chewiness than the other treatments.In conclusion,quadratic regression model analysis based on SGR and IgM revealed that the optimum xanthophyll level in the diet was 86.78 and 84.63 mg/kg,respectively.Moreover,broken line regression analysis based on xanthophyll content in dorsal skin and chewiness in muscle demonstrated that the optimal xanthophyll level was between 89.73 and 108.66 mg/kg in the diet of southern catfish(S.soldatovi meridionalis Chen).展开更多
主要探索不同饲料组成对鱼体组成的影响。将360尾南方鲇（Silurus meridionalis Chen）幼鱼随机分成24组，饲养于24个水泥池中；共设计了21种饲料；实验持续了40d。结果表明：（1）不同营养素水平，对鱼体中的蛋白质沉积无明显影响；肌...主要探索不同饲料组成对鱼体组成的影响。将360尾南方鲇（Silurus meridionalis Chen）幼鱼随机分成24组，饲养于24个水泥池中；共设计了21种饲料；实验持续了40d。结果表明：（1）不同营养素水平，对鱼体中的蛋白质沉积无明显影响；肌肉中脂肪水平随着饲料中脂肪和碳水化合物（CBH）水平的增加而增加；CBH含量为33％时，肌肉中的CBH的沉积量显著高于其他几个组（P〈0．05）；（2）脏体比和肠脂比随着饲料中脂肪水平升高而升高，脂肪水平9％～15％，肝脏中沉积的脂肪含量随着饲料中脂肪含量的增加而增加，添加CBH含量为33％的饲料时，肝脏中糖原的沉积含量显著的高于其他几个CBH含量的组（P〈0．05）。展开更多
采用水体染毒法研究了南方鲇(Silurus meridionalis Chen)幼鱼Cd暴露0、7、14d时肝脏中总抗氧化能力(TAOC)、金属硫蛋白(MT)含量、丙二醛(MDA)含量的变化以及血液中血红蛋白(Hb)含量、脑中胆碱酯酶(AChE)活性的变化。实验设置1个对照组...采用水体染毒法研究了南方鲇(Silurus meridionalis Chen)幼鱼Cd暴露0、7、14d时肝脏中总抗氧化能力(TAOC)、金属硫蛋白(MT)含量、丙二醛(MDA)含量的变化以及血液中血红蛋白(Hb)含量、脑中胆碱酯酶(AChE)活性的变化。实验设置1个对照组和4个不同Cd含量(0.015、0.06、0.24、0.96 mg·L-1)的处理组。结果显示,Hb含量和AChE活性先显著性降低(p<0.05)然后恢复到正常水平;T-AOC水平和MT含量的变化为随Cd含量升高和暴露时间延长而显著性升高和增加(p<0.05);MDA含量在暴露14d时显著性降低(p<0.05)。研究提示,AChE活性和Hb含量先受到Cd暴露影响,而南方鲇通过肝脏增加合成MT、提高T-AOC水平来对抗Cd的毒害;较长时间的Cd暴露使肝脏MDA含量降低,同时合成MT的能力和T-AOC水平明显升高,表现出"毒物兴奋效应";南方鲇可通过调节体内生理功能使之应对环境中一定范围的Cd污染胁迫。展开更多
基金supported by the Foundation and Applied Basic Research Fund Project of Guangdong Province(2019A1515110606)Innovation Group Project of Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai).
文摘Xanthophyll has multiple physiological functions to improve the quality of farmed animals.The present study aimed to investigate the effects of xanthophyll on the growth performance,antioxidation,immunity,pigmentation and meat quality of southern catfish(Silurus soldatovi meridionalis Chen).Juvenile southern catfish(18.35±0.04 g)were randomly allocated into 24 cages(30 juveniles per cage),and fed diets with different dietary xanthophyll levels(at 14,42,80,108,126 and 152 mg/kg,dry matter of diet)twice daily for 8 weeks.Results indicated that the diet with 80 mg/kg xanthophyll induced a higher specific growth rate(SGR),feed efficiency ratio and protein productive value.Moreover,the 80 mg/kg xanthophyll diet also increased complement 3,immunoglobulin M(IgM)and lysozyme content more than the other groups.The mRNA expression level of inflammation-related genes and antioxidant enzyme activities further confirmed the effects of 80 mg/kg dietary xanthophyll on improving immune response.The present study also found that the 126 mg/kg xanthophyll diet significantly enhanced the content of total carotenoids and xanthophyll,hydroxyproline,collagen and amino acid in muscle.The diet with 126 mg/kg xanthophylls also induced lower drip loss,thawing loss,centrifugal loss,cooking loss and higher muscle adhesiveness,cohesiveness,springiness,gumminess and chewiness than the other treatments.In conclusion,quadratic regression model analysis based on SGR and IgM revealed that the optimum xanthophyll level in the diet was 86.78 and 84.63 mg/kg,respectively.Moreover,broken line regression analysis based on xanthophyll content in dorsal skin and chewiness in muscle demonstrated that the optimal xanthophyll level was between 89.73 and 108.66 mg/kg in the diet of southern catfish(S.soldatovi meridionalis Chen).
文摘主要探索不同饲料组成对鱼体组成的影响。将360尾南方鲇（Silurus meridionalis Chen）幼鱼随机分成24组，饲养于24个水泥池中；共设计了21种饲料；实验持续了40d。结果表明：（1）不同营养素水平，对鱼体中的蛋白质沉积无明显影响；肌肉中脂肪水平随着饲料中脂肪和碳水化合物（CBH）水平的增加而增加；CBH含量为33％时，肌肉中的CBH的沉积量显著高于其他几个组（P〈0．05）；（2）脏体比和肠脂比随着饲料中脂肪水平升高而升高，脂肪水平9％～15％，肝脏中沉积的脂肪含量随着饲料中脂肪含量的增加而增加，添加CBH含量为33％的饲料时，肝脏中糖原的沉积含量显著的高于其他几个CBH含量的组（P〈0．05）。
文摘采用水体染毒法研究了南方鲇(Silurus meridionalis Chen)幼鱼Cd暴露0、7、14d时肝脏中总抗氧化能力(TAOC)、金属硫蛋白(MT)含量、丙二醛(MDA)含量的变化以及血液中血红蛋白(Hb)含量、脑中胆碱酯酶(AChE)活性的变化。实验设置1个对照组和4个不同Cd含量(0.015、0.06、0.24、0.96 mg·L-1)的处理组。结果显示,Hb含量和AChE活性先显著性降低(p<0.05)然后恢复到正常水平;T-AOC水平和MT含量的变化为随Cd含量升高和暴露时间延长而显著性升高和增加(p<0.05);MDA含量在暴露14d时显著性降低(p<0.05)。研究提示,AChE活性和Hb含量先受到Cd暴露影响,而南方鲇通过肝脏增加合成MT、提高T-AOC水平来对抗Cd的毒害;较长时间的Cd暴露使肝脏MDA含量降低,同时合成MT的能力和T-AOC水平明显升高,表现出"毒物兴奋效应";南方鲇可通过调节体内生理功能使之应对环境中一定范围的Cd污染胁迫。
