Biochar and bio-oil are produced simultaneously in one pyrolysis process,and they inevitably contact and may interact,influencing the composition of bio-oil and modifying the structure of biochar.In this sense,biochar...Biochar and bio-oil are produced simultaneously in one pyrolysis process,and they inevitably contact and may interact,influencing the composition of bio-oil and modifying the structure of biochar.In this sense,biochar is an inherent catalyst for pyrolysis.In this study,in order to investigate the influence of functionalities and pore structures of biochar on its capability for catalyzing the conversion of homologous volatiles in bio-oil,three char catalysts(600C,800C,and 800AC)produced via pyrolysis of poplar wood at 600 or 800℃or activated at 800℃,were used for catalyzing pyrolysis of homologous poplar wood at 600℃,respectively.The results indicated that the 600C catalyst was more active than 800C and 800AC for catalyzing cracking of volatiles to form more gas(yield increase by 40.2%)and aromatization of volatiles to form more light or heavy phenolics,due to its abundant oxygen-containing functionalities acting as active sites.The developed pores of the 800AC showed no such catalytic effect but could trap some volatiles and allow their further conversion via sufficient aromatization.Nevertheless,the interaction with the volatiles consumed oxygen on 600C(decrease by 50%),enhancing the aromatic degree and increasing thermal stability.The dominance of deposition of carbonaceous material of a very aromatic nature over 800C and 800AC resulted in net weight gain and blocked micropores but formed additional macropores.The in situ diffuse reflectance infrared Fourier transform spectroscopy characterization of the catalytic pyrolysis indicated superior activity of 600C for removal of -OH,while conversion of the intermediates bearing C=O was enhanced over all the char catalysts.展开更多
Wet wood is an abnormal phenomenon in growing trees,which adversely affects growth,subsequent wood processing and economic values of wood products.In this study,the influences of factors such as clones,afforestation m...Wet wood is an abnormal phenomenon in growing trees,which adversely affects growth,subsequent wood processing and economic values of wood products.In this study,the influences of factors such as clones,afforestation methods,site conditions and climate conditions on the characteristics of poplar wetwood were studied through field investigations in 27 clones from 48 sample plots in 28 counties.Results showed that the incidences of wetwood were almost 100%in all plots.Ratios of wetwood area among the48 plots differed from 15.1 to 90.2%.Wetwood area ratios,moisture contents and pH differed significantly between the 27 clones.Wetwood area ratios of the clones ranged from18.7 to 62.3%.Ratios of wetwood areas were positively correlated with wet wood moisture content and pH,tree age,and negatively correlated with pH of sap wood.The repeatability of wet wood area ratios was 0.52,moderately controlled by genetics.Wetwood moisture content and pH were highly controlled by genetics,indicated by the repeatability of 0.91 and 0.89,respectively.There were significant differences in wetwood area ratios,moisture content and pH between different site conditions,afforestation methods,and geographical regions.Sloping land had the lowest wetwood area ratios and moisture content among four types of sites.Afforestation by direct seeding and rooted cuttings had the lowest wetwood area ratios and moisture content,respectively.In the three geographical regions,the Yellow River Basin had the lowest wetwood values of all three factors.展开更多
The nuclear-encoded factors and the photosynthetic apparatus have been studied extensively during chloroplast biogenesis.However,many questions regarding these processes remain unanswered,particularly in perennial woo...The nuclear-encoded factors and the photosynthetic apparatus have been studied extensively during chloroplast biogenesis.However,many questions regarding these processes remain unanswered,particularly in perennial woody plants.As a model material of woody plants,poplar not only has very significant value of research,but also possesses economic and ecological properties.This study reports the Populus trichocarpa DJ-1C(PtrDJ1C)factor,encoded by a nuclear gene,and a member of the DJ-1 superfamily.PtrDJ1C knock-out with the CRISPR/Cas9 system resulted in different albino phenotypes.Chlorophyll fluorescence and immunoblot analyses showed that the levels of photosynthetic complex proteins decreased significantly.Moreover,the transcript level of plastid-encoded RNA polymerase-dependent genes and the splicing efficiency of several introns were affected in the mutant line.Furthermore,rRNA accumulation was abnormal,leading to developmental defects in chloroplasts and affecting lignin accumulation.We concluded that the PtrDJ1C protein is essential for early chloroplast development and lignin deposition in poplar.展开更多
Sodium silicate modification can improve the overall performance of wood.The modification process has a great influence on the properties of modified wood.In this study,a new method was introduced to analyze the wood ...Sodium silicate modification can improve the overall performance of wood.The modification process has a great influence on the properties of modified wood.In this study,a new method was introduced to analyze the wood modification process,and the properties of modified wood were studied.Poplar wood was modified with sodium silicate by vacuum-pressure impregnation.After screening using single-factor experiments,an orthogonal experiment was carried out with solution concentration,impregnation time,impregnation pressure,and the cycle times as experimental factors.The modified poplar with the best properties was selected by fuzzy mathematics and characterized by SEM,FT-IR,XRD and TG.The results showed that some lignin and hemicellulose were removed from the wood due to the alkaline action of sodium silicate,and the orderly crystal area of poplar became disorderly,resulting in the reduction of crystallinity of the modified poplar wood.FT-IR analysis showed that sodium silicate was hydrolyzed to form polysilicic acid in wood,and structural analysis revealed the formation of Si-O-Si and Si-O-C,indicating that sodium silicate reacted with fibers on the wood cell wall.TG-DTG curves showed that the final residual mass of modified poplar wood increased from 25%to 67%,and the temperature of the maximum loss rate decreased from 343℃ to 276℃.The heat release and smoke release of modified poplar wood decreased obviously.This kind of material with high strength and fire resistance can be used in the outdoor building and indoor furniture.展开更多
Drought seriously affects the growth and development of plants.MiR159 is a highly conserved and abundant microRNA family that plays a crucial role in plant growth and stress responses.However,studies of its function i...Drought seriously affects the growth and development of plants.MiR159 is a highly conserved and abundant microRNA family that plays a crucial role in plant growth and stress responses.However,studies of its function in woody plants are still lacking.Here,the expression of miR159a was significantly upregulated after drought treatment in poplar,and the overexpression of miR159a(OX159a)significantly reduced the open area of the stomata and improved water-use efficiency in poplar.After drought treatment,OX159a lines had better scavenging ability of reactive oxygen species and damage of the membrane system was less than that in wild-type lines.MYB was the target gene of miR159a,as verified by psRNATarget prediction,RT-qPCR,degradome sequencing,and 5′rapid amplification of cDNA ends(5′RACE).Additionally,miR159a-short tandem target mimic suppression(STTM)poplar lines showed increased sensitivity to drought stress.Transcriptomic analysis comparing OX159a lines with wild-type lines revealed upregulation of a series of genes related to response to water deprivation and metabolite synthesis.Moreover,drought-responsive miR172d and miR398 were significantly upregulated and downregulated respectively in OX159a lines.This investigation demonstrated that miR159a played a key role in the tolerance of poplar to drought by reducing stomata open area,increasing the number and total area of xylem vessels,and enhancing water-use efficiency,and provided new insights into the role of plant miR159a and crucial candidate genes for the molecular breeding of trees with tolerance to drought stress.展开更多
Fracture is a common failure form of poplar laminated veneer lumber(LVL).In the present work,we performed an experimental study on the mode-I along-grain interlaminar fracture,mode-I cross-grain interlaminar fracture,...Fracture is a common failure form of poplar laminated veneer lumber(LVL).In the present work,we performed an experimental study on the mode-I along-grain interlaminar fracture,mode-I cross-grain interlaminar fracture,and mode-II interlaminar fracture of poplar LVL.We investigated stress mechanisms,failure modes,and fracture toughness values of the different fracture types.The experimental results revealed that the crack in the mode-I along-grain interlaminar fracture specimen propagated along the prefabricated crack direction,and the crack tip broke.The mode-I cross-grain interlaminar fracture specimen had cracks in the vertical direction near the prefabricated crack.In the mode-II interlaminar fracture specimen,cracks appeared along the initial prefabricated crack direction.The load–displacement curves of these three specimens were linear in the early stage of loading.With the increase in the load,a nonlinear segment appeared before crack propagation and a descending segment appeared after crack propagation.The nonlinear segments of the mode-I along-grain interlaminar fracture and mode-II interlaminar fracture were very short,and cracks expanded quickly after their initiation,resulting in brit-tle fracture.The nonlinear segment of the mode-I cross-grain interlaminar fracture was long,resulting in plastic failure.The average toughness values of the mode-I along-grain interlaminar fracture,mode-I cross-grain inter-laminar fracture,and mode-II interlaminar fracture were 15.43,270.15,and 39.72 MPa·mm^(1/2),respectively.展开更多
Poplar wood,which was used as the absorption material for the solar-driven interfacial evaporation,was treated for 3 days,6 days and 9 days with the pectinase,and then was simulated for photothermal evaporation test a...Poplar wood,which was used as the absorption material for the solar-driven interfacial evaporation,was treated for 3 days,6 days and 9 days with the pectinase,and then was simulated for photothermal evaporation test at one standard solar radiation intensity(1 kW⋅m^(−2)).The effects of pectinase treatment on cell passage and water migration capacity of poplars were analyzed by the mercury intrusion porosimetry,the scanning electron microscope and fractal theory.It was found that the pit membrane and the ray parenchyma cells of poplar wood were degraded and destroyed after pectinase treatment.Compared with the untreated poplar wood,the evaporation rate of three sections of the specimen was changed.Especially the evaporation rate of radial and tangential direction was significantly increased.At the same time,based on the experimental data and fractal dimension deduction,fractal characteristics could be found in that the structure of poplars treated with pectinase.The porosity decreased with the increase of the fractal dimension in a certain range.It was shown that it is feasible to evaluate solar-driven water migration capacity by using fractal theory.展开更多
Partially modified Bt Cry1Ac gene and the arrowhead proteinase inhibitor (API) gene were used to construct a plant transformation vector pBtiA and this construct was transferred into the genome of the hybrid popla...Partially modified Bt Cry1Ac gene and the arrowhead proteinase inhibitor (API) gene were used to construct a plant transformation vector pBtiA and this construct was transferred into the genome of the hybrid poplar 741 [ Populus alba L.×( P. davidiana Dode+ P. simonii Carr.)× P. tomentosa Carr.] by Agrobacterium _ mediated transformation. Ten kanamycin resistant plants have been regenerated. Upon insect bioassay using Clostera anachoreta (Fabricius), three of the examined plants were demonstrated to be highly resistant to the testing insects. The mortality of insect larvae on one plant was higher than 90% in 6 days after infestation and the growth of the survival larvae were seriously inhibited. Results of PCR and Southern blot analysis indicated that both Bt Cry1Ac gene and API gene were integrated as a single copy into the genomes of these three plants when Cry1Ac gene fragment was used as the probe. Protein dot blot immunoassay and ELISA analysis revealed that at least the Cry1Ac protein was produced in these three transgenic plants and the expression levels were estimated to be approximately 0.015% of the leaf total soluble protein. This is the first report on insect resistant transgenic hybrid poplar 741 that expresses two insecticidal protein genes.展开更多
Codon usage is the selective and nonrandom use of synonymous codons to encode amino acids in genes for proteins. The analysis of codon usage may improve the understanding of cocion preferences between different specie...Codon usage is the selective and nonrandom use of synonymous codons to encode amino acids in genes for proteins. The analysis of codon usage may improve the understanding of cocion preferences between different species and allow to rebuild the codons of exogenous genes to increase the expression efficiency of exogenous genes, Here, codon DNA sequence (CDS) of four poplar species, including Populus tremuloides Michx., P. tomentosa Carr., P. deltoides Marsh., and P. trichocarpa Torr. & Gray., is used to analyze the relative frequency of synonymous codon (RFSC). High-frequency codons are selected by high-frequency (HF) codon analysis. The results indicate that the codon usage is common for all four poplar species and the codon preference is quite similar among the four poplar species. However, CCT encoding for Pro, and ACT coding for Thr are the preferred codons in P. tremuloides and P. tomentosa, whereas CCA coding for Pro, and ACA coding for Thr are preferred in P. deltoides and P. trichocarpa The codons such as TGC coding for Cys, TTC coding for Phe, and AAG coding for Lys, are preferred in the poplar species except P trichocarpa. GAG coding for Glu is preferred only in P deltoides, while the other three poplar species prefer to use GAA. The commonness of preferred codon allows exogenous gene designed by the preferred cocion of one of the different poplar species to be used in other poplar species.展开更多
The cDNA fragment encoding caffeic acid 3_O_methyltransferase (COMT) in Chinese white poplar ( Populus tomentosa Carr.) was isolated and cloned by RT_PCR technique. The size of the cDNA fragment is 1 080 bp, which alm...The cDNA fragment encoding caffeic acid 3_O_methyltransferase (COMT) in Chinese white poplar ( Populus tomentosa Carr.) was isolated and cloned by RT_PCR technique. The size of the cDNA fragment is 1 080 bp, which almost covers the whole cDNA_encoding region. Authors’ cDNA fragment in P. tomentosa shares 98.7% homology with the reported corresponding cDNA in the P. tremuloids at nucleotide level, 99.4% homology at amino acid level, respectively. The analysis of Northern dot hybridization showed that COMT is expressed specifically in the developing secondary xylem of stem during the season of xylem differentiation, which means the linkage between the gene expression for a monolignol biosynthetic enzyme and seasonal regulation of xylem development in woody plant.展开更多
基金supported by the National Natural Science Foundation of China(51876080)the Program for Taishan Scholars of the Shandong Province Government。
文摘Biochar and bio-oil are produced simultaneously in one pyrolysis process,and they inevitably contact and may interact,influencing the composition of bio-oil and modifying the structure of biochar.In this sense,biochar is an inherent catalyst for pyrolysis.In this study,in order to investigate the influence of functionalities and pore structures of biochar on its capability for catalyzing the conversion of homologous volatiles in bio-oil,three char catalysts(600C,800C,and 800AC)produced via pyrolysis of poplar wood at 600 or 800℃or activated at 800℃,were used for catalyzing pyrolysis of homologous poplar wood at 600℃,respectively.The results indicated that the 600C catalyst was more active than 800C and 800AC for catalyzing cracking of volatiles to form more gas(yield increase by 40.2%)and aromatization of volatiles to form more light or heavy phenolics,due to its abundant oxygen-containing functionalities acting as active sites.The developed pores of the 800AC showed no such catalytic effect but could trap some volatiles and allow their further conversion via sufficient aromatization.Nevertheless,the interaction with the volatiles consumed oxygen on 600C(decrease by 50%),enhancing the aromatic degree and increasing thermal stability.The dominance of deposition of carbonaceous material of a very aromatic nature over 800C and 800AC resulted in net weight gain and blocked micropores but formed additional macropores.The in situ diffuse reflectance infrared Fourier transform spectroscopy characterization of the catalytic pyrolysis indicated superior activity of 600C for removal of -OH,while conversion of the intermediates bearing C=O was enhanced over all the char catalysts.
基金funded by the Hubei Provincial Natural Science Foundation of China (2021CFB446)the National Natural Science Foundation of China (31570665)+1 种基金the National Key Research and Development Program of China (2021YFD2201202)the Fundamental Research Funds for the Central Universities (2662020YLPY017)。
文摘Wet wood is an abnormal phenomenon in growing trees,which adversely affects growth,subsequent wood processing and economic values of wood products.In this study,the influences of factors such as clones,afforestation methods,site conditions and climate conditions on the characteristics of poplar wetwood were studied through field investigations in 27 clones from 48 sample plots in 28 counties.Results showed that the incidences of wetwood were almost 100%in all plots.Ratios of wetwood area among the48 plots differed from 15.1 to 90.2%.Wetwood area ratios,moisture contents and pH differed significantly between the 27 clones.Wetwood area ratios of the clones ranged from18.7 to 62.3%.Ratios of wetwood areas were positively correlated with wet wood moisture content and pH,tree age,and negatively correlated with pH of sap wood.The repeatability of wet wood area ratios was 0.52,moderately controlled by genetics.Wetwood moisture content and pH were highly controlled by genetics,indicated by the repeatability of 0.91 and 0.89,respectively.There were significant differences in wetwood area ratios,moisture content and pH between different site conditions,afforestation methods,and geographical regions.Sloping land had the lowest wetwood area ratios and moisture content among four types of sites.Afforestation by direct seeding and rooted cuttings had the lowest wetwood area ratios and moisture content,respectively.In the three geographical regions,the Yellow River Basin had the lowest wetwood values of all three factors.
基金supported by the National Natural Science Foundation of China(Grant Nos.32201516,91954202)the Youth Top-notch Talent Program of Hebei Education Department(BJK2022028)+1 种基金National Training Program of Innovation and Entrepreneurship for Undergraduates(Grant Nos.S202110022037,G202010022075)the funding of Hebei North University(XJ2021013)。
文摘The nuclear-encoded factors and the photosynthetic apparatus have been studied extensively during chloroplast biogenesis.However,many questions regarding these processes remain unanswered,particularly in perennial woody plants.As a model material of woody plants,poplar not only has very significant value of research,but also possesses economic and ecological properties.This study reports the Populus trichocarpa DJ-1C(PtrDJ1C)factor,encoded by a nuclear gene,and a member of the DJ-1 superfamily.PtrDJ1C knock-out with the CRISPR/Cas9 system resulted in different albino phenotypes.Chlorophyll fluorescence and immunoblot analyses showed that the levels of photosynthetic complex proteins decreased significantly.Moreover,the transcript level of plastid-encoded RNA polymerase-dependent genes and the splicing efficiency of several introns were affected in the mutant line.Furthermore,rRNA accumulation was abnormal,leading to developmental defects in chloroplasts and affecting lignin accumulation.We concluded that the PtrDJ1C protein is essential for early chloroplast development and lignin deposition in poplar.
基金This work was financially supported by National Natural Science Foundation of China(32201485)Natural Science Foundation of Hunan Province,China(2022JJ40863)+1 种基金Scientific Research Project of Hunan Provincial Education Department,China(21B0238)The Science and Technology Innovation Program of Hunan Province(2021RC4062).
文摘Sodium silicate modification can improve the overall performance of wood.The modification process has a great influence on the properties of modified wood.In this study,a new method was introduced to analyze the wood modification process,and the properties of modified wood were studied.Poplar wood was modified with sodium silicate by vacuum-pressure impregnation.After screening using single-factor experiments,an orthogonal experiment was carried out with solution concentration,impregnation time,impregnation pressure,and the cycle times as experimental factors.The modified poplar with the best properties was selected by fuzzy mathematics and characterized by SEM,FT-IR,XRD and TG.The results showed that some lignin and hemicellulose were removed from the wood due to the alkaline action of sodium silicate,and the orderly crystal area of poplar became disorderly,resulting in the reduction of crystallinity of the modified poplar wood.FT-IR analysis showed that sodium silicate was hydrolyzed to form polysilicic acid in wood,and structural analysis revealed the formation of Si-O-Si and Si-O-C,indicating that sodium silicate reacted with fibers on the wood cell wall.TG-DTG curves showed that the final residual mass of modified poplar wood increased from 25%to 67%,and the temperature of the maximum loss rate decreased from 343℃ to 276℃.The heat release and smoke release of modified poplar wood decreased obviously.This kind of material with high strength and fire resistance can be used in the outdoor building and indoor furniture.
基金This work was supported by the National Natural Science Foundation of China(32371577)the Beijing Natural Science Foundation(6232030)the Major Project of Agricultural Biological Breeding(2022ZD0401503).
文摘Drought seriously affects the growth and development of plants.MiR159 is a highly conserved and abundant microRNA family that plays a crucial role in plant growth and stress responses.However,studies of its function in woody plants are still lacking.Here,the expression of miR159a was significantly upregulated after drought treatment in poplar,and the overexpression of miR159a(OX159a)significantly reduced the open area of the stomata and improved water-use efficiency in poplar.After drought treatment,OX159a lines had better scavenging ability of reactive oxygen species and damage of the membrane system was less than that in wild-type lines.MYB was the target gene of miR159a,as verified by psRNATarget prediction,RT-qPCR,degradome sequencing,and 5′rapid amplification of cDNA ends(5′RACE).Additionally,miR159a-short tandem target mimic suppression(STTM)poplar lines showed increased sensitivity to drought stress.Transcriptomic analysis comparing OX159a lines with wild-type lines revealed upregulation of a series of genes related to response to water deprivation and metabolite synthesis.Moreover,drought-responsive miR172d and miR398 were significantly upregulated and downregulated respectively in OX159a lines.This investigation demonstrated that miR159a played a key role in the tolerance of poplar to drought by reducing stomata open area,increasing the number and total area of xylem vessels,and enhancing water-use efficiency,and provided new insights into the role of plant miR159a and crucial candidate genes for the molecular breeding of trees with tolerance to drought stress.
基金the National Natural Science Foundation of China(Grant No.51878590)the Jiangsu Provincial Department of Housing and Construction(Grant Nos.2019ZD092,2020ZD40,2020ZD42)the Sixth Phase of“333 Project”Training Objects in Jiangsu Province and Jiangsu Province High-Level Talent Selection Training(Grant No.JNHB-127)for their financial support.
文摘Fracture is a common failure form of poplar laminated veneer lumber(LVL).In the present work,we performed an experimental study on the mode-I along-grain interlaminar fracture,mode-I cross-grain interlaminar fracture,and mode-II interlaminar fracture of poplar LVL.We investigated stress mechanisms,failure modes,and fracture toughness values of the different fracture types.The experimental results revealed that the crack in the mode-I along-grain interlaminar fracture specimen propagated along the prefabricated crack direction,and the crack tip broke.The mode-I cross-grain interlaminar fracture specimen had cracks in the vertical direction near the prefabricated crack.In the mode-II interlaminar fracture specimen,cracks appeared along the initial prefabricated crack direction.The load–displacement curves of these three specimens were linear in the early stage of loading.With the increase in the load,a nonlinear segment appeared before crack propagation and a descending segment appeared after crack propagation.The nonlinear segments of the mode-I along-grain interlaminar fracture and mode-II interlaminar fracture were very short,and cracks expanded quickly after their initiation,resulting in brit-tle fracture.The nonlinear segment of the mode-I cross-grain interlaminar fracture was long,resulting in plastic failure.The average toughness values of the mode-I along-grain interlaminar fracture,mode-I cross-grain inter-laminar fracture,and mode-II interlaminar fracture were 15.43,270.15,and 39.72 MPa·mm^(1/2),respectively.
基金The authors’funding is supported by the Fundamental Research Funds Jiangsu Vocational College of Agriculture and Forestry(2021KJ20).
文摘Poplar wood,which was used as the absorption material for the solar-driven interfacial evaporation,was treated for 3 days,6 days and 9 days with the pectinase,and then was simulated for photothermal evaporation test at one standard solar radiation intensity(1 kW⋅m^(−2)).The effects of pectinase treatment on cell passage and water migration capacity of poplars were analyzed by the mercury intrusion porosimetry,the scanning electron microscope and fractal theory.It was found that the pit membrane and the ray parenchyma cells of poplar wood were degraded and destroyed after pectinase treatment.Compared with the untreated poplar wood,the evaporation rate of three sections of the specimen was changed.Especially the evaporation rate of radial and tangential direction was significantly increased.At the same time,based on the experimental data and fractal dimension deduction,fractal characteristics could be found in that the structure of poplars treated with pectinase.The porosity decreased with the increase of the fractal dimension in a certain range.It was shown that it is feasible to evaluate solar-driven water migration capacity by using fractal theory.
基金ThePresidentialFoundationofTheChineseAcademyofSciences NaturalScienceFoundationofHebeiProvince China
文摘Partially modified Bt Cry1Ac gene and the arrowhead proteinase inhibitor (API) gene were used to construct a plant transformation vector pBtiA and this construct was transferred into the genome of the hybrid poplar 741 [ Populus alba L.×( P. davidiana Dode+ P. simonii Carr.)× P. tomentosa Carr.] by Agrobacterium _ mediated transformation. Ten kanamycin resistant plants have been regenerated. Upon insect bioassay using Clostera anachoreta (Fabricius), three of the examined plants were demonstrated to be highly resistant to the testing insects. The mortality of insect larvae on one plant was higher than 90% in 6 days after infestation and the growth of the survival larvae were seriously inhibited. Results of PCR and Southern blot analysis indicated that both Bt Cry1Ac gene and API gene were integrated as a single copy into the genomes of these three plants when Cry1Ac gene fragment was used as the probe. Protein dot blot immunoassay and ELISA analysis revealed that at least the Cry1Ac protein was produced in these three transgenic plants and the expression levels were estimated to be approximately 0.015% of the leaf total soluble protein. This is the first report on insect resistant transgenic hybrid poplar 741 that expresses two insecticidal protein genes.
基金This work was supported by the National Major Basic Research and Development Program(No.TG1999016004)Jiangsu Provincial Natural Science Foundation(No.BK2003213).
文摘Codon usage is the selective and nonrandom use of synonymous codons to encode amino acids in genes for proteins. The analysis of codon usage may improve the understanding of cocion preferences between different species and allow to rebuild the codons of exogenous genes to increase the expression efficiency of exogenous genes, Here, codon DNA sequence (CDS) of four poplar species, including Populus tremuloides Michx., P. tomentosa Carr., P. deltoides Marsh., and P. trichocarpa Torr. & Gray., is used to analyze the relative frequency of synonymous codon (RFSC). High-frequency codons are selected by high-frequency (HF) codon analysis. The results indicate that the codon usage is common for all four poplar species and the codon preference is quite similar among the four poplar species. However, CCT encoding for Pro, and ACT coding for Thr are the preferred codons in P. tremuloides and P. tomentosa, whereas CCA coding for Pro, and ACA coding for Thr are preferred in P. deltoides and P. trichocarpa The codons such as TGC coding for Cys, TTC coding for Phe, and AAG coding for Lys, are preferred in the poplar species except P trichocarpa. GAG coding for Glu is preferred only in P deltoides, while the other three poplar species prefer to use GAA. The commonness of preferred codon allows exogenous gene designed by the preferred cocion of one of the different poplar species to be used in other poplar species.
文摘The cDNA fragment encoding caffeic acid 3_O_methyltransferase (COMT) in Chinese white poplar ( Populus tomentosa Carr.) was isolated and cloned by RT_PCR technique. The size of the cDNA fragment is 1 080 bp, which almost covers the whole cDNA_encoding region. Authors’ cDNA fragment in P. tomentosa shares 98.7% homology with the reported corresponding cDNA in the P. tremuloids at nucleotide level, 99.4% homology at amino acid level, respectively. The analysis of Northern dot hybridization showed that COMT is expressed specifically in the developing secondary xylem of stem during the season of xylem differentiation, which means the linkage between the gene expression for a monolignol biosynthetic enzyme and seasonal regulation of xylem development in woody plant.