Soy molasses and soy solubles are byproducts of the conventional soy protein concentrate and soy protein isolate manufacturing processes,respectively.Conversion of the carbohydrates in these byproducts into ethanol wa...Soy molasses and soy solubles are byproducts of the conventional soy protein concentrate and soy protein isolate manufacturing processes,respectively.Conversion of the carbohydrates in these byproducts into ethanol was examined.Standardized amounts of commercial cellulase enzymes(Novozyme cellulase,β-glucosidase,and pectinase)were added to soy molasses and soy solubles solutions prepared at various solid loading rates(33%,50%,60%,75%,and 80%)to hydrolyze oligosaccharides,followed by fermentation for 96 h using Saccharomyces cerevisiae NRRL Y-2034 and Scheffersomyces stipitis NRRL Y-7124.Ethanol-extracted soybean meal(SBM)carbohydrates were also fermented for 96 h without enzymes.S.cerevisiae and S.stipitis produced about 12.5-45.0 g/L and 6.0-28.0 g/L ethanol,respectively,on molasses and solubles across these solid loading rates.The S.stipitis produced about 6.5-17 g/L ethanol and S.cerevisiae produced about 6.5-22 g/L ethanol on ethanol-extracted carbohydrates.展开更多
文摘Soy molasses and soy solubles are byproducts of the conventional soy protein concentrate and soy protein isolate manufacturing processes,respectively.Conversion of the carbohydrates in these byproducts into ethanol was examined.Standardized amounts of commercial cellulase enzymes(Novozyme cellulase,β-glucosidase,and pectinase)were added to soy molasses and soy solubles solutions prepared at various solid loading rates(33%,50%,60%,75%,and 80%)to hydrolyze oligosaccharides,followed by fermentation for 96 h using Saccharomyces cerevisiae NRRL Y-2034 and Scheffersomyces stipitis NRRL Y-7124.Ethanol-extracted soybean meal(SBM)carbohydrates were also fermented for 96 h without enzymes.S.cerevisiae and S.stipitis produced about 12.5-45.0 g/L and 6.0-28.0 g/L ethanol,respectively,on molasses and solubles across these solid loading rates.The S.stipitis produced about 6.5-17 g/L ethanol and S.cerevisiae produced about 6.5-22 g/L ethanol on ethanol-extracted carbohydrates.
