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Genome-wide association with transcriptomics reveals a shade-tolerance gene network in soybean
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作者 Yanzhu Su Xiaoshuai Hao +11 位作者 Weiying Zeng Zhenguang Lai Yongpeng Pan Can Wang Pengfei Guo Zhipeng Zhang Jianbo He Guangnan Xing Wubin Wang Jiaoping Zhang Zudong Sun Junyi Gai 《The Crop Journal》 SCIE CSCD 2024年第1期232-243,共12页
Shade tolerance is essential for soybeans in inter/relay cropping systems.A genome-wide association study(GWAS)integrated with transcriptome sequencing was performed to identify genes and construct a genetic network g... Shade tolerance is essential for soybeans in inter/relay cropping systems.A genome-wide association study(GWAS)integrated with transcriptome sequencing was performed to identify genes and construct a genetic network governing the trait in a set of recombinant inbred lines derived from two soybean parents with contrasting shade tolerance.An improved GWAS procedure,restricted two-stage multi-locus genome-wide association study based on gene/allele sequence markers(GASM-RTM-GWAS),identified 140 genes and their alleles associated with shade-tolerance index(STI),146 with relative pith cell length(RCL),and nine with both.Annotation of these genes by biological categories allowed the construction of a protein–protein interaction network by 187 genes,of which half were differentially expressed under shading and non-shading conditions as well as at different growth stages.From the identified genes,three ones jointly identified for both traits by both GWAS and transcriptome and two genes with maximum links were chosen as beginners for entrance into the network.Altogether,both STI and RCL gene systems worked for shade-tolerance with genes interacted each other,this confirmed that shadetolerance is regulated by more than single group of interacted genes,involving multiple biological functions as a gene network. 展开更多
关键词 soybean(glycine max(l.)merr.) SHADE-TOlERANCE Restricted two-stage multi-locus genomewide association study based on gene/allele sequence markers(gASM-RTM-gWAS) Shade-tolerance index(STI) Relative cell length(RCl) Transcriptome
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A novel procedure for identifying a hybrid QTL-allele system for hybrid-vigor improvement, with a case study in soybean(Glycine max)yield
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作者 Jinshe Wang Jianbo He +1 位作者 Jiayin Yang Junyi Gai 《The Crop Journal》 SCIE CSCD 2023年第1期177-188,共12页
“Breeding by design” for pure lines may be achieved by construction of an additive QTL-allele matrix in a germplasm panel or breeding population, but this option is not available for hybrids, where both additive and... “Breeding by design” for pure lines may be achieved by construction of an additive QTL-allele matrix in a germplasm panel or breeding population, but this option is not available for hybrids, where both additive and dominance QTL-allele matrices must be constructed. In this study, a hybrid-QTL identification approach, designated PLSRGA, using partial least squares regression(PLSR) for model fitting integrated with a genetic algorithm(GA) for variable selection based on a multi-locus, multi-allele model is described for additive and dominance QTL-allele detection in a diallel hybrid population(DHP). The PLSRGA was shown by simulation experiments to be superior to single-marker analysis and was then used for QTL-allele identification in a soybean DPH yield experiment with eight parents. Twenty-eight main-effect QTL with 138 alleles and nine QTL × environment QTL with 46 alleles were identified, with respective contributions of 61.8% and 23.5% of phenotypic variation. Main-effect additive and dominance QTL-allele matrices were established as a compact form of the DHP genetic structure. The mechanism of heterosis superior-to-parents(or superior-to-parents heterosis, SPH) was explored and might be explained by a complementary locus-set composed of OD+(showing positive over-dominance, most often), PD+(showing positive partial-to-complete dominance, less often) and HA+(showing positive homozygous additivity, occasionally) loci, depending on the parental materials. Any locus-type, whether OD+, PD + and HA+, could be the best genotype of a locus. All hybrids showed various numbers of better or best genotypes at many but not necessarily all loci, indicating further SPH improvement. Based on the additive/dominance QTL-allele matrices, the best hybrid genotype was predicted, and a hybrid improvement approach is suggested. PLSRGA is powerful for hybrid QTL-allele detection and cross-SPH improvement. 展开更多
关键词 Breeding by design Diallel hybrid population PlSRgA(partial least squares regression via genetic algorithm) QTl-allele matrix of additive/dominance effect Simulation experiment soybean[glycine max(l.)merr.]
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Analysis of QTLs for the Trichome Density on the Upper and Downer Surface of Leaf Blade in Soybean [Glycine max (L.) Merr.] 被引量:6
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作者 DU Wei-jun YU De-yue FU San-xiong 《Agricultural Sciences in China》 CAS CSCD 2009年第5期529-537,共9页
Trichomes (plant hairs) are present on nearly all land plants and are known to play important roles in plant protection, specifically against insect herbivory, drought, and UV radiation. The identification of quanti... Trichomes (plant hairs) are present on nearly all land plants and are known to play important roles in plant protection, specifically against insect herbivory, drought, and UV radiation. The identification of quantitative trait loci (QTL) associated with trichome density should help to interpret the molecular genetic mechanism of soybean trichome density. 184 recombinant inbred lines (RILs), derived from a cross between soybean cultivars Kefeng 1 and Nannong 1138-2 were used as segregating population for evaluation of TDU (trichome density on the upper surface of leaf blade) and TDD (trichome density on the downer surface of leaf blade). A total of 15 QTL were detected on molecular linkage groups (MLG) A2, Dla, Dlb, E and H by composite interval mapping (CIM) and among all the QTL, qtuA2-1, qtuD 1 a-1, qtuD lb-2, qtuH-2 qtuE-1, qtdDlb-2, and qtdH- 2 were affirmed by multiple interval mapping (MIM). The contribution ofphenotypic variance of qtuH-2 was 31.81 and 29.4% by CIM and MIM, respectively, suggesting it might be major gene Ps loci. Only 10 pairs of main QTL interactions for TDU were detected, explained a range of 0.2-5.1% of phenotypic variations for each pair for a total of 22.8%. The QTL on MLG Dlb affecting trichome density were mapped near to Rsc-7 conditioning resistance to SMV (soybean mosaic virus). This study showed that the genetic mechanism of trichome density was the mixed major gene and polygene inheritance, and also suggested that the causal nature between trichome density and other agronomic traits. 展开更多
关键词 soybean [glycine max l.) merr.] TDU TDD RI ls QTl analysis
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Genetic Analysis of Combining Abilities and Heterosis for the Contents of Soybean Isoflavone and Its Components Among the Soybean Varieties [Glycine max (L.) Merr.] 被引量:2
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作者 LIANGHui-zhen LIWei-dong +1 位作者 WANGHui FANGXuan-jun 《Agricultural Sciences in China》 CAS CSCD 2005年第7期555-560,共6页
The genetic analysis of soybean isoflavone content and its components were carried out based on the NC Ⅱ matingdesign in eight soybean varieties. The results showed that the isoflavone contents and its components of ... The genetic analysis of soybean isoflavone content and its components were carried out based on the NC Ⅱ matingdesign in eight soybean varieties. The results showed that the isoflavone contents and its components of soybean seedare quite differences among the tested materials, the contents of isoflavone and daidzein are controlled not only byadditive effects and but also by non-additive effects, while the content of genistin is dominated by non-additive effects,and genistein, glycitin and daidzin are mainly controlled by additive effects. There are significant differences in thecontents of isoflavone and its components among the combinations derived from different parents. Results also indicatedthat the tested traits are negatively heterosis except for the contens of daidzein and daidzin are positively heterosis basedon the data of the GCA and SCA in average heterosis values. In this research we have a suggestion that soybean varietywith high isoflavone should be used as one of the parents in the breeding program, and it is the best choice that thecombinations crossed between two high isoflavone varieties or a high variety and a low one. 展开更多
关键词 soybean[glycine max (l.) merr.] ISOFlAVONE Component of isoflavone Combining ability HETEROSIS
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Genetic Analysis of Embryo, Cytoplasm and Maternal Effects and Their Environment Interactions for Isoflavone Content in Soybean [Glycine max (L.) Merr.] 被引量:2
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作者 LIANG Hui-zhen WANG Shu-feng +3 位作者 WANG Ting-feng ZHANG Hai-yang ZHAO Shuang-jin ZHANG Meng-chen 《Agricultural Sciences in China》 CAS CSCD 2007年第9期1051-1059,共9页
Soybean seed products contain isoflavones (genistein, daidzein, and glycitein) that display biological effects when ingested by humans and animals. These effects are species, dose and age dependent. Therefore, the c... Soybean seed products contain isoflavones (genistein, daidzein, and glycitein) that display biological effects when ingested by humans and animals. These effects are species, dose and age dependent. Therefore, the content and quality of isoflavones in soybeans is a key factor to the biological effect. Our objective was to identify the genetic effects that underlie the isoflavone content in soybean seeds. A genetic model for quantitative traits of seeds in diploid plants was applied to estimate the genetic main effects and genotype x environment (GE) interaction effects for the isoflavone content (IC) of soybean seeds by using two years experimental data with an incomplete diallel mating design of six parents. Results showed that the IC of soybean seeds was simultaneously controlled by the genetic effects of maternal, embryo, and cytoplasm, of which maternal genetic effects were most important, followed by embryo and cytoplasmic genetic effects. The main effects of different genetic systems on IC trait were more important than environment interaction effects. The strong dominance effects on isoflavone from residual was made easily by environment conditions. Therefore, the improvement of the IC of soybean seeds would be more efficient when selection is based on maternal plants than that on the single seed. Maternal heritability (65.73%) was most important for IC, followed by embryo heritability (25.87%) and cytoplasmic heritability (8.39%). Based on predicated genetic effects, Yudou 29 and Zheng 90007 were better than other parents for increasing IC in the progeny and improving the quality of soybean, The significant effects of maternal and embryo dominance effects in variance show that the embryo heterosis and maternal heterosis are existent and uninfluenced by environment interaction effects. 展开更多
关键词 isoflavone content (IC) glycine max l.) merr. soybean genetic effect HERITABIlITY
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Proteomic Studies of Petal-specific Proteins in Soybean [Glycine Max(L.)Merr.] Florets
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作者 Guo Fang-liang Liu Han-miao +7 位作者 Luo Ting-ting Fang Si-jia Pang Ze Yang Ming-ming Wei Xiao-shuang Song Bo Liu Shan-shan Li Wen-bin 《Journal of Northeast Agricultural University(English Edition)》 CAS 2019年第1期10-18,共9页
A survey of petal-specific proteomes of soybean(Glycine max(L.) Merr[Non-italic].) was conducted comparing protein expression profiles in different petals. Two-dimensional polyacrylamide gel electrophoresis reference ... A survey of petal-specific proteomes of soybean(Glycine max(L.) Merr[Non-italic].) was conducted comparing protein expression profiles in different petals. Two-dimensional polyacrylamide gel electrophoresis reference maps of protein extracts from standard petals(SP), lateral wings(LW), keel petals(KP), and reproductive organs(RO)(a mixture of stamen and carpel) were obtained. Protein expression in the three petal types was compared using Image Master TM 2 D platinum 6.0 software. This indicated that the proportion of homologous proteins between SP and LW was 59.27%, between SP and KP was 61.48%, and between LW and KP was 60.05%. Within a mass range of 6.5-200.0 ku and pH 4.0-7.0, approximately 590, 646, 544, and 700 protein spots were detected in SP, LW, KP, and RO, respectively. A total of 82 differentially expressed proteins were detected. Sixty-four of these detected spots were differentially expressed and showed more than 2-fold changes in abundance; of these 64 proteins, 26 showed increased expression and 38 showed decreased expression. Among these spots, single organ-specific proteins were also identified.They were ID 49(60.9 ku), ID 45(50.0 ku), and ID 46(40.5 ku) in RO, ID 98(42.0 ku) in SP, and ID 05(29.0 ku) in KP. A total of 14 protein spots from 82 differentially expressed proteins were identified with LC-MS/MS. Further protein identification was conducted using the SwissProt and NCBInr databases. The identified proteins and their putative functions were discussed further. This was the first study reporting the comparison of petal protein profiles of soybean florets using proteomics tools. 展开更多
关键词 soybean(glycine max(l.) merr.) 2D-PAgE lC-MS/MS PETAl protein
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大豆(Glycine max(L.) Merr.)叶片抗氧化酶对干旱及复水的响应机制 被引量:9
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作者 王文斌 王晓怡 +2 位作者 张明辉 胡晓婕 栗相如 《山西农业大学学报(自然科学版)》 CAS 2012年第3期193-197,共5页
采用10%PEG-6000模拟干旱胁迫,研究了抗旱品种晋大74与不抗旱品种晋大75两种大豆幼苗在水分胁迫及复水后叶片MDA含量、O2-·含量、SOD、CAT、APX及POD等的活性变化,旨在揭示抗氧化酶对干旱及复水的响应机制。结果表明,在干旱胁迫下... 采用10%PEG-6000模拟干旱胁迫,研究了抗旱品种晋大74与不抗旱品种晋大75两种大豆幼苗在水分胁迫及复水后叶片MDA含量、O2-·含量、SOD、CAT、APX及POD等的活性变化,旨在揭示抗氧化酶对干旱及复水的响应机制。结果表明,在干旱胁迫下,两种大豆叶片MDA及O2-·,含量都高于对照,且晋大75增加幅度显著大于晋大74;复水后晋大74中MDA及O2-·接近于对照水平,表明旱后复水产生了补偿效应。干旱胁迫下,晋大74通过保持较高的SOD活性或较低的APX、CAT降幅以减弱活性氧伤害,而旱后复水过程中晋大74通过维持较高的SOD、APX、CAT酶活性,利用其协同作用有效清除活性氧,避免膜伤害。总之,耐旱品种在干旱及复水过程中具有更强的抗氧化修复能力。 展开更多
关键词 大豆 干旱 复水 抗氧化酶 补偿效应
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野生大豆(Glycine soja)YD63和栽培大豆(G. max)ZD19茎秆解剖结构比较 被引量:2
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作者 要燕杰 油清波 +8 位作者 赵为 郭葳 沈欣杰 李祥 张永兴 周蓉 赵剑 周新安 矫永庆 《中国油料作物学报》 CAS CSCD 北大核心 2018年第2期199-208,共10页
以野生大豆YD63和栽培大豆ZD19为研究对象,通过组织化学方法观察茎秆解剖结构的差异,分析和阐述这些解剖结构与功能和环境适应性间的关系,旨在为大豆抗逆性研究提供解剖学依据。结果表明:1.野生大豆表皮毛和腺毛多于栽培大豆,且角质层... 以野生大豆YD63和栽培大豆ZD19为研究对象,通过组织化学方法观察茎秆解剖结构的差异,分析和阐述这些解剖结构与功能和环境适应性间的关系,旨在为大豆抗逆性研究提供解剖学依据。结果表明:1.野生大豆表皮毛和腺毛多于栽培大豆,且角质层厚度、表皮厚度和表皮比例均大于栽培大豆,表皮和外皮层细胞的木质化和木栓化程度也高于栽培大豆;2.野生大豆皮层、韧皮部、木薄壁组织和髓的比例均大于栽培大豆,茎秆机械强度降低,可塑性升高,抗逆性增强;3.栽培大豆木质部、木纤维和总纤维比例均大于野生大豆,并且表皮细胞壁、韧皮纤维壁、木纤维壁和导管壁厚度均大于野生大豆。栽培大豆组织木质化的比例大于野生大豆,茎秆的机械强度升高,可以更好地维持直立生长和形态构建;4.栽培大豆微管形成层的细胞层数和厚度均大于野生大豆。栽培大豆木质部的比例大于韧皮部的比例,而野生大豆两者比例基本相同;5.野生大豆韧皮部厚壁组织几乎是连续分布,仅在髓射线处中断,而栽培大豆是不连续的,呈片状分布,野生大豆韧皮部厚壁组织的比例大于栽培大豆;6.野生大豆导管壁强度(t/b)2和小导管比例大于栽培大豆,水分运输的安全性较高,但野生大豆木质部的连通性和水分运输的效率低于栽培大豆。 展开更多
关键词 野生大豆 栽培大豆 解剖结构 进化 抗性
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Sampling Survey and Identification of Races of Soybean Cyst Nematode (Heterodera glycines Ichinohe) in Huang-Huai Valleys 被引量:5
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作者 LU Wei-guo GAI Jun-yi LI Wei-dong 《Agricultural Sciences in China》 CAS CSCD 2006年第8期615-621,共7页
Soybean cyst nematode (SCN Heterodera glycines Ichinohe) is one of the most important nationwide soybean diseases in China. A total of 38 soil specimens or locations in the area was sampled and tested for SCN races ... Soybean cyst nematode (SCN Heterodera glycines Ichinohe) is one of the most important nationwide soybean diseases in China. A total of 38 soil specimens or locations in the area was sampled and tested for SCN races during 2001-2003 for the inspection of race distribution in Huang-Huai Valleys. A map of race distribution was constructed according to the data from both the present study and the published reports cited. Three areas, namely, the area of southeast to Jinan in Shangdong Province; the area of northern Henan Province and its border region to south of Hebei Province; and the area of Luohe, Zhoukou of Henan Province and Fuyang of Anhui Province mainly infested with Race 1 were identified. Race 4 was predominant in Shanxi Province, Beijing and the adjacent area of Henan, Shandong, and Anhui provinces, and the delta of Huanghe River in Shandong Province. Race 2 was mainly found in Liaocheng, Dezhou of Shangdong Province and Shijiazhuang of Hebei Province, and Jiaozuo and Huojia of Henan Province. Race 7 was distributed in the west part of Jiaodong Peninsula of Shandong Province and Kaifeng, Huaxian, Wenxian of Henan Province. Race 5 was found and scattered in Hebei and Henan Province. Race 9 was found in Shangqiu of Henan Province, which was reported for the first time in China. It can be seen that Race 1 and Race 4 were the two predominant races in Huang-Huai Valleys, and that research should focus on developing resistant cultivars of these races. There might exist other races in an area with some predominant races. The race substitution in the past decade was not obviously found, therefore, the results should be meaningful to future breeding for resistance to SCN in Huang-Huai Valleys. 展开更多
关键词 soybean (glycine max l.) merr.) Huang-Huai Valleys cyst nematode (Heterodera glycines Ichinohe) race
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Synergistic interaction of NaCl and Cd on growth and photosynthetic parameters in soybean genotypes differing in salinity tolerance 被引量:3
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作者 SHAMSI Imran Haider 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2007年第4期266-271,共6页
The effects of salinity (50 mmol/L NaCI) and Cd (1 μmol/L CdCl2) as sole and combined on growth and photosynthetic parameters were studied using two soybean genotypes, Huachun 18 and NGB. The concentrations of C... The effects of salinity (50 mmol/L NaCI) and Cd (1 μmol/L CdCl2) as sole and combined on growth and photosynthetic parameters were studied using two soybean genotypes, Huachun 18 and NGB. The concentrations of Cd^2+, Zn^2+, Ca^2+, Mg^2+, K^+ and Na^+were also determined in seeds and pods. Huachun 18 suffered a more serious decrease than NGB in net photosynthetic rate (P,) in the treatments of salinity stress alone and combined stress (NaCl+Cd), showing that it is relatively sensitive to salinity. The decrease in P, caused by salt stress in Huachun 18 was mainly due to the reduced total chlorophyll content and photosynthetic efficiency (the ratio of variable fluorescence to maximal fluorescence, Fv/Fm), whereas the decease in NGB was mainly related to reduced stomatal conductance (Gs), The combined stress of both Na and Cd did not induce further decrease in photosynthesis and fluorescence in the two genotypes relative to salt or Cd stress alone. Greater change in the pod concentrations of Zn^2+, Ca^2+, Mg^2+, K^+ and Na^+was detected under salt stress for Huachun 18 than for NGB. The results suggested that the interactive effect of NaCl-Cd on growth and nutrient uptake differs between the two soybean genotypes. 展开更多
关键词 soybean (glycine max l.) merr.) SAlINITY Cadmium PHOTOSYNTHESIS Fluorescence
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Effect of Preplant Fertilizer on Agronomic and Physiological Traits of Soybean Cultivars from Different Breeding Programs 被引量:3
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作者 XIE Fu-ti ZHANG Hui-jun +2 位作者 WANG Hai-ying AO Xue St. Martin Steven K 《Agricultural Sciences in China》 CSCD 2010年第11期1602-1611,共10页
Understanding the changes in agronomic and physiological traits associated with yield genetic gain is important for soybean production and future breeding strategy. The objective of this study was to compare the older... Understanding the changes in agronomic and physiological traits associated with yield genetic gain is important for soybean production and future breeding strategy. The objective of this study was to compare the older and modern cultivars to learn whether the yield improvements depend on preplant fertilizer or the plant productivity improvement, A set of older cultivars, with their modern counterparts derived from breeding programs in Liaoning and Ohio were evaluated for their agronomic and physiological traits under different fertilizer levels from 2004 to 2006. There was no improvement of response to N and P preplant fertilizer for genotypes. After more than 70 yr breeding, soybean breeders made some improvements in agronomic and physiological traits that contribute to yield increase. When compared to older cultivar, modern Liaoning and Ohio cultivars were shorter and more resistance to lodging, had greater leaf density, higher harvest index, more leaf area per plant, and greater photosynthetic rate, transpiration rate and stomatal conductance at the beginning of seed development. Ohio cultivars were more resistant to lodging as if selected for easy harvest by combine, even under high N and P preplant fertilizer level, which resulted in Ohio cultivars with higher and stable yield productivity. 展开更多
关键词 soybean [giycine max l.) merr.] FERTIlIZER agronomic trait PHYSIOlOgY BREEDINg
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A novel TIR-NBS-LRR gene regulates immune response to Phytophthora root rot in soybean 被引量:2
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作者 Li Zhou Sushuang Deng +6 位作者 Huidong Xuan Xingxing Fan Ruidong Sun Jinming Zhao Haitang Wang Na Guo Han Xing 《The Crop Journal》 SCIE CSCD 2022年第6期1644-1653,共10页
Phytophthora root rot(PRR),caused by Phytophthora sojae,is a devastating disease of soybean.The NBSLRR gene family is a class of plant genes involved in disease resistance.miRNA mediates plant response to biotic stres... Phytophthora root rot(PRR),caused by Phytophthora sojae,is a devastating disease of soybean.The NBSLRR gene family is a class of plant genes involved in disease resistance.miRNA mediates plant response to biotic stresses by regulating the expression of target genes at the transcriptional or post-translational level.Glyma.16G135500,encoding an NBS-LRR-type protein,is a target of gma-miR1510 that responds to pathogen infections.We cloned and overexpressed Glyma.16G135500(naming it GmTNL16)and knocked down mi R1510 using short tandem target mimic technology to identify the roles of the GmTNL16/gma-mi R1510 pair in the interaction of soybean and the oomycete.By overexpressing GmTNL16 in transgenic hairy roots of soybean,we showed that biomass of P.sojae was lower in overexpressing hairy roots than in control roots.Thus,miR1510 expression was reduced upon P.sojae infection,reflecting the induced expression of GmTNL16 conferring resistance to P.sojae in soybean.Differentially expressed genes were enriched in plant-pathogen interaction,plant hormone signal transduction,and secondary metabolism by RNA sequencing analyze.In particular,jasmonate and salicylic acid pathway-associated genes,including JAZ,COI1,TGA,and PR,responded to P.sojae infection.All of these results indicate that the GmTNL16/gma-miR1510 pair participates in soybean defense response via the JA and SA pathways. 展开更多
关键词 soybean(glycine max(l.)merr.) Phytophthora sojae gma-miR1510 gmTNl16 SA and JA pathway
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Effect of Plant Density on Modern Soybean Cultivars Released from Ohio and Liaoning
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作者 谢甫绨 St.MARTIN STEVEN K. 《江西农业大学学报》 CAS CSCD 北大核心 2010年第5期895-900,共6页
Seeding rate is an important management practice for soybean production.Chinese and U.S.soybean growers use different seeding rates,and breeders in the two countries have developed cultivars adapted to respective plan... Seeding rate is an important management practice for soybean production.Chinese and U.S.soybean growers use different seeding rates,and breeders in the two countries have developed cultivars adapted to respective plant densities.The objective of this study was to compare the effect of plant density on cultivars recently released in different breeding programs,using four cultivars developed in Liaoning,China and four in Ohio,USA.We used 3 plant density treatments(7.5,15.0,22.5 x 104 plants/hm2) and assessed yield and agronomic traits from 2004 to 2006 in Liaoning.There was no significant effect of plant density on yield for either group of the cultivars.The average yield of Ohio cultivars was higher than that of Liaoning cultivars,and there was no significant interaction between plant density and cultivar for all the assessed traits.The plant height of Liaoning cultivars was significantly higher than that of Ohio cultivars,and there was a significant effect of plant density on plant height.The average branch number of Ohio cultivars was larger than that of Liaoning cultivars;higher plant density reduced the branch number per plant greatly.Plant density had a signifi-cant effect on the node number and internode length,Liaoning cultivars generally had longer internode length.Plant density had a significant effect on seed yield:stem ratio,as the plant density increased the seed yield:stem ratio decreased for both groups of cultivars.However,100-seed weight was not affected by plant density. 展开更多
关键词 soybean [glycine max(l.) merr.] BREEDINg planting density agronomic trait
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大豆抗筛豆龟蝽Megacota cribraria(Fabricius)的QTL分析 被引量:8
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作者 邢光南 周斌 +4 位作者 赵团结 喻德跃 邢邯 陈受宜 盖钧镒 《作物学报》 CAS CSCD 北大核心 2008年第3期361-368,共8页
筛豆龟蝽是我国南方大豆的主要害虫之一,本研究旨在定位筛豆龟蝽抗性QTL,分析其稳定性,为大豆抗筛豆龟蝽育种提供参考。以科丰1号×南农1138-2组合衍生的含184个重组自交系的群体NJRIKY(简称KY)和皖82-178×通山薄皮黄豆甲组... 筛豆龟蝽是我国南方大豆的主要害虫之一,本研究旨在定位筛豆龟蝽抗性QTL,分析其稳定性,为大豆抗筛豆龟蝽育种提供参考。以科丰1号×南农1138-2组合衍生的含184个重组自交系的群体NJRIKY(简称KY)和皖82-178×通山薄皮黄豆甲组合衍生的含142个重组自交系的群体NJRIWT(简称WT)为材料,2004-2006在田间自然虫源下鉴定了筛豆龟蝽抗性。不同年份内以黑霉程度为指标的方差分析结果表明家系间差异在每年都达极显著水平,遗传变异系数都相当大,遗传率中等偏高。利用Windows QTL Cartographer Version2.5的复合区间作图法(CIM),KY群体的抗性QTL主要位于D1a和C2连锁群,WT群体的抗性QTL主要位于H和D1b连锁群。KY群体3年均检测出的qRMC-d1a-1位于D1a连锁群,贡献率为7.6%~31.4%;2005和2006两年均检测出的qRMC-c2-1位于C2连锁群,与环境有互作,效应相对较小;抗性等位基因来自南农1138-2;qRMC-d1a-1和qRMC-h-1在2005年和2006年存在显著的互作。WT群体连锁群H上的qRMC-h-1在3年中都被检测到,贡献率为16.3%~36.2%;D1b连锁群上的qRMC-d1b-2在2004年和2005年被检测到,效应相对较小;抗性等位基因来自通山薄皮黄豆甲。虽然WT群体D1b和H连锁群上的这2个QTL在KY群体中也有一年被检测到,但2个群体抗性位点基本上是不同的。QTL在不同环境被重复检出,说明大豆对筛豆龟蝽的抗性由稳定的主效QTL所控制,其2侧邻近标记有希望用于标记辅助选择育种。 展开更多
关键词 大豆[glycine max(l.)merr.] 筛豆龟蝽[Megacota cribraria(Fabricius)] 抗虫性 QTl定位
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大豆低植酸突变体Gm-lpa-ZC-2的精细定位 被引量:2
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作者 李佰权 姜莹 +3 位作者 付旭军 朱申龙 朱丹华 袁凤杰 《浙江农业学报》 CSCD 北大核心 2011年第5期870-875,共6页
降低大豆植酸含量对于改善大豆营养品质极其重要。通过诱变的方法筛选到大豆低植酸突变体Gm-lpaa-ZC-2,经典遗传学分析表明:其突变性状由隐性单基因所控制,并被定位在大豆连锁群B2上。试验在大豆低植酸突变体Gm-lpa—ZC-2初步定位的... 降低大豆植酸含量对于改善大豆营养品质极其重要。通过诱变的方法筛选到大豆低植酸突变体Gm-lpaa-ZC-2,经典遗传学分析表明:其突变性状由隐性单基因所控制,并被定位在大豆连锁群B2上。试验在大豆低植酸突变体Gm-lpa—ZC-2初步定位的基础上,通过构建较大的遗传群体,运用生物信息学发展新的连锁标记,结合大豆基因组学和传统定位的方法,进行突变基因的精确定位。结果表明:位于大豆B2连锁群上的Gm-lpa-ZC-2突变基凶,与连锁标记Satt168和Satt416的遗传距离远大于初步定位的结果,为18.2cM;4E此基础上,利用大豆基因组序列信息,在Satt168标记的上游序列与目的基因之间的染色体片段,4900~8200kb的区域中,发掘到9对在遗传群体亲本间具有多态性的SSR引物;其中SSR位点psmlpa-224,psmlpa-225和psmlpa-226与目的基因的距离仅为0.8cM,实现了突变基凶的精细定位。 展开更多
关键词 大豆[glycine max l.merr.] 低植酸 突变体 精细定位
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GmAGL15基因启动子的克隆及序列分析
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作者 郭伟 汪潇淋 +1 位作者 王慧 喻德跃 《大豆科学》 CAS CSCD 北大核心 2008年第5期727-731,共5页
为探明大豆GmAGL15基因的表达调控规律,应用电子克隆技术从大豆基因组中克隆了GmAGL15 1000 bp的启动子序列,用PLACE在线启动子预测工具分析表明:该序列含有启动子的一般结构,如TATA-BOX、CAAT-BOX。另外还含有一些顺式作用元件,如调控G... 为探明大豆GmAGL15基因的表达调控规律,应用电子克隆技术从大豆基因组中克隆了GmAGL15 1000 bp的启动子序列,用PLACE在线启动子预测工具分析表明:该序列含有启动子的一般结构,如TATA-BOX、CAAT-BOX。另外还含有一些顺式作用元件,如调控GmAGL15的组织特异的和特定发育阶段的表达,对胁迫的应答,对光的响应,以及反馈调节,推测大豆GmAGL15基因表达有相应组织特异性,可能受蔗糖、生长素和乙烯等的调控。用FootPrinter和PLACE在线工具对大豆与拟南芥等其他4种植物的同源基因启动子的顺式元件进行比较,发现不同植物的启动子既有保守性,又有多样性,转录因子结合位点的分布相似,但也有区别,暗示了GmAGL15基因表达调控的精确性或多样性。 展开更多
关键词 大豆 gmAgl15 启动子 电子克隆 序列分析
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RTM-GWAS方法应用于大豆RIL群体百粒重QTL检测的功效 被引量:8
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作者 潘丽媛 贺建波 +7 位作者 赵晋铭 王吴彬 邢光南 喻德跃 张小燕 李春燕 陈受宜 盖钧镒 《中国农业科学》 CAS CSCD 北大核心 2020年第9期1730-1742,共13页
【目的】为全面解析大豆重组自交系群体中调控百粒重性状的QTL体系,将限制性两阶段多位点全基因组关联分析方法(RTM-GWAS)和不同定位方法进行比较、优选,为后续候选基因体系探索及分子标记辅助育种设计提供依据。【方法】利用以科丰1号... 【目的】为全面解析大豆重组自交系群体中调控百粒重性状的QTL体系,将限制性两阶段多位点全基因组关联分析方法(RTM-GWAS)和不同定位方法进行比较、优选,为后续候选基因体系探索及分子标记辅助育种设计提供依据。【方法】利用以科丰1号和南农1138-2为亲本衍生的重组自交系群体NJRIKY的427个家系,通过由全基因组39353个SNP构建的3683个SNPLDB标记及3个环境下的百粒重表型数据,选用复合区间作图法(CIM)、基于混合线性模型的全基因组关联分析方法(MLM-GWAS)和RTM-GWAS 3种方法检测百粒重QTL,通过QTL数目和总的表型变异解释率比较检测功效,挑选最佳定位结果进行NJRIKY群体中的百粒重遗传体系解析。通过候选基因体系的功能注释,挖掘调控大豆百粒重的生物学途径。【结果】科丰1号与南农1138-2的百粒重差异较大,多环境平均数分别为9.0和17.9 g,遗传变异系数为12.4%,遗传率为85.4%,适用于百粒重性状的遗传解析。比较3种方法定位结果表明RTM-GWAS方法表现最佳,检测QTL数目最多(57个),解释表型变异最多(70.78%)。而CIM仅检测到14个QTL,解释了56.47%的表型变异,MLM-GWAS仅定位到6个QTL,解释了18.47%的表型变异。RTM-GWAS共检测到57个QTL,分布在19条染色体上,表型变异解释率为0.03%—7.57%,其中41个QTL覆盖了已报道的来自30个双亲群体的81个百粒重QTL,16个QTL为新发现位点,包含一个表型变异解释率大于3%的大效应位点Sw-09-2。此外,检测的57个QTL中有20个位点与环境存在互作效应。这57个QTL构成了影响NJRIKY群体百粒重性状的遗传体系。通过SNPLDB标记与预测基因内的SNP进行χ^2检验,共筛选到36个候选基因,其中4个候选基因来自大效应QTL,剩余32个候选基因来自小效应QTL。通过GO注释发现这些候选基因功能注释丰富,其中13个候选基因与籽粒发育直接相关,剩余的候选基因功能丰富,包含转运、转录调节因子等,表明不同生物学途径的基因共同调控NJRIKY群体中百粒重性状的表达。【结论】3种定位方法中,高效的RTM-GWAS方法检测到较为全面的NJRIKY群体的百粒重QTL,更适用于双亲RIL群体的QTL定位。不同功能的候选基因共同调控了复杂的百粒重性状的表达。 展开更多
关键词 大豆 百粒重 QTl 重组自交家系 限制性两阶段多位点全基因组关联分析
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Effect of Phosphorus Fertilization to P Uptake and Dry Matter Accumulation in Soybean with Different P Efficiencies 被引量:12
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作者 AO Xue GUO Xiao-hong +6 位作者 ZHU Qian ZHANG Hui-jun WANG Hai-ying MA Zhao-hui HAN Xiao-ri ZHAO Ming-hui XIE Fu-ti 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2014年第2期326-334,共9页
Phosphorus (P) is an essential element for plant growth and yield. Improving phosphorus use efficiency of crops could potentially reduce the application of chemical fertilizer and alleviate environmental damage. Soy... Phosphorus (P) is an essential element for plant growth and yield. Improving phosphorus use efficiency of crops could potentially reduce the application of chemical fertilizer and alleviate environmental damage. Soybean (Glycine max (L.) Merr.) is sensitive to phosphorus (P) in the whole life history. Soybean cultivars with different P efficiencies were used to study P uptake and dry matter accumulation under different P levels. Under low P conditions, the P contents of leaf in high P efficiency cultivars were greater than those in low P efficiency cultivars at the branching stage. The P accumulation in stems of high P efficiency cultivars and in leaves of low P efficiency cultivars increased with increasing P concentration at the branching stage. At the late podding stage, the P accumulation of seeds in high and low P efficiency cultivars were 22.5 and 26.0%, respectively; and at the mature stage were 69.8 and 74.2%, respectively. In average, the P accumulation in whole plants and each organ was improved by 24.4% in high P efficiency cultivars compared to low P efficiency cultivars. The biomass between high and low P efficiency cultivars were the same under extended P condition, while a significant difference was observed at late pod filling stage. At the pod setting stage, the biomass of high P efficiency cultivars were significant greater (17.4%) than those of low P efficiency cultivars under high P condition. Meanwhile, under optimum growth conditions, there was little difference ofbiomass between the two types of cultivars, however, the P agronomic efficiency and P harvest index were significant higher in high P efficiency cultivars than those in low P efficiency cultivars. 展开更多
关键词 glycine max l.) merr. soybean phosphorus use efficiency
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Identification of a novel seed size associated locus SW9-1 in soybean 被引量:5
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作者 Jiajia Li Jinghui Zhao +8 位作者 Yinghui Li Yali Gao Sunan Hua Muhammad Nadeem Genlou Sun Wenming Zhang Jinfeng Hou Xiaobo Wang Lijuan Qiu 《The Crop Journal》 SCIE CAS CSCD 2019年第4期548-559,共12页
Seed size is one of the vital traits determining seed appearance, quality, and yield. Untangling the genetic mechanisms regulating soybean 100-seed weight (100-SW), seed length and seed width across environments may p... Seed size is one of the vital traits determining seed appearance, quality, and yield. Untangling the genetic mechanisms regulating soybean 100-seed weight (100-SW), seed length and seed width across environments may provide a theoretical basis for improving seed yield. However, there are few reports related to QTL mapping of 100-SW across multiple ecological regions. In this study, 21 loci associated with seed size traits were identified using a genome-wide association of 5361 single nucleotide polymorphisms (SNPs) across three ecoregions in China, which could explain 8.12%–14.25% of the phenotypic variance respectively. A new locus, named as SW9-1 on chromosome 9 that explained 10.05%–10.93% of the seed weight variance was found significantly related to seed size traits, and was not previously reported. The selection effect analysis showed that SW9-1 locus has a relatively high phenotypic effect (13.67) on 100-SW, with a greater contribution by the accessions with bigger seeds (3.69) than the accessions with small seeds (1.66). Increases in seed weight were accompanied by increases in the frequency of SW9-1T allele, with >90% of the bred varieties with a 100-SW >30 g carrying SW9-1T. Analysis of SW9-1 allelic variation in additional soybean accessions showed that SW9-1T allele accounting for 13.83% of the wild accessions, while in 46.55% and 51.57% of the landraces and bred accessions, respectively, this results indicating that the SW9-1 locus has been subjected to artificial selection during the early stages of soybean breeding, especially the utilization of SW9-1T in edamame for big seed. These results suggest that SW9-1 is a novel and reliable locus associated with seed size traits, and might have an important implication for increasing soybean seed weight in molecular design breeding. Cloning this locus in future may provide new insights into the genetic mechanisms underlying soybean seed size traits. 展开更多
关键词 soybean [glycine max (l.) merr.] SEED size/weight SW9-1 Molecular BREEDINg
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Constitutive expression of feedback-insensitive cystathionine γ-synthase increases methionine levels in soybean leaves and seeds 被引量:4
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作者 YU Yang HOU Wen-sheng +6 位作者 Yael Hacham SUN Shi WU Cun-xiang Ifat Matityahu SONG Shi-kui Rachel Amir HAN Tian-fu 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2018年第1期54-62,共9页
Soybean (Glycine max (L.) Merr.) is a major crop that provides plant-origin protein and oil for humans and livestock. Al- though the soybean vegetative tissues and seeds provide a major source of high-quality prot... Soybean (Glycine max (L.) Merr.) is a major crop that provides plant-origin protein and oil for humans and livestock. Al- though the soybean vegetative tissues and seeds provide a major source of high-quality protein, they suffer from low con- centration of an essential sulfur-containing amino acid, methionine, which significantly limits their nutritional quality. The level of methionine is mainly controlled by the first unique enzyme of methionine synthesis, cystathione y-synthase (CGS). Aiming to elevate methionine level in vegetative tissues and seeds, we constitutively over-expressed a feedback-insensitive Arabidopsis CGS (AtD-CGS) in soybean cultivars, Zigongdongdou (ZD) and Jilinxiaoli 1 (JX). The levels of soluble methionine increased remarkably in leaves of transgenic soybeans compared to wild-type plants (6.6- and 7.3-fold in two transgenic ZD lines, and 3.7-fold in one transgenic JX line). Furthermore, the total methionine contents were significantly increased in seeds of the transgenic ZD lines (1.5- to 4.8-fold increase) and the transgenic JX lines (1.3- to 2.3-fold increase) than in the wild type. The protein contents of the transgenic soybean seeds were significantly elevated compared to the wild type, suggesting that the scarcity of methionine in soybeans may limit protein accumulation in soybean seeds. The increased protein content did not alter the profile of major storage proteins in the seeds. Generally, this study provides a promising strategy to increase the levels of methionine and protein in soybean through the breeding programs. 展开更多
关键词 soybean (glycine max l.) merr.) constitutive expression cystathionine y-synthase (CgS) methionine contentstorage protein
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