The soybean cultivar Tadang Muangpai was used to improve the productive quality of Thua-Nao and reduce the concentration of aflatoxin to less than 20 ppb. It was conducted at CMFCRC, Chiangmai, Thailand between Dec. 2...The soybean cultivar Tadang Muangpai was used to improve the productive quality of Thua-Nao and reduce the concentration of aflatoxin to less than 20 ppb. It was conducted at CMFCRC, Chiangmai, Thailand between Dec. 2006 and Mar. 2007. Soybean was boiled for 5 hours and then fermented at different time to create natural bacterial species, mainly Bacillus spp. Thua-Nao could be stored up to 90 days after in storage. Nutritional value, food value, and microorganisms content were investigated during fermentation and storage. Also, aflatoxin content of Thua-Nao was recorded during storage. The results showed that 3 days of soybean fermentation gave the best performance of Thua-Nao in term of nutritional value (protein = 47.12%), food value, and content of Bacillus spp. (2.78 × 10^9 CFU/g). Without being harmed from aflatoxin, Thua-Nao could be stored not more than 23 days in normal room (Tmax. = 33.9 ℃, Tmin. = 15.8 ℃) and not more than 36 days in climate-controlled room (Tmax. = 20 ℃, Tmin. = 15 ℃).展开更多
Background: Soybean meal is an excellent and cost-effective protein source; however, its usage is limited in the piglet due to the presence of anti-nutritional factors and the antigens glycinin and β-conglycinin. The...Background: Soybean meal is an excellent and cost-effective protein source; however, its usage is limited in the piglet due to the presence of anti-nutritional factors and the antigens glycinin and β-conglycinin. The objective of the current study was to screen and select for bacteria that can be efficiently adopted to ferment soybean meal in order to solve this problem.Results: Bacteria were isolated from fermented soy foods and the grass carp intestine, and strains selected for high protease, cellulase and amylase activities. The isolated bacteria were characterized as Bacillus cereus, Bacillus subtilis and Bacilus amyloliquefacien, respectively. Fermentation with food-derived Isolate-2 and fish-derived F-9 increased crude protein content by 5.32% and 8.27%, respectively; improved the amino acid profile by increasing certain essential amino acids, broke down larger soy protein to 35 k Da and under, eliminated antigenicity against glycinin and β-conglycinin, and removed raffinose and stachyose in the soybean meal following a 24-h fermentation.Conclusions: Our results suggest these two B. amyloliquefaciens bacteria can efficiently solid state ferment soybean meal and ultimately produce a more utilizable food source for growing healthy piglets.展开更多
[Objective] This study aimed to evaluate tbe healthy risk of genetically modified ( GM ) soybeans by using a novel approach for functions and safety of food. [ Me^od] Different from traditional evaluation of substan...[Objective] This study aimed to evaluate tbe healthy risk of genetically modified ( GM ) soybeans by using a novel approach for functions and safety of food. [ Me^od] Different from traditional evaluation of substantial equivalence, three great innovations were performed in this study, involving in basic diet, evalu- ation approaches and principle, as well as the clarification of connotation differences between absolute and relative mass of organs. Hence a novel BDI-GS (Bendib Damage Index and General Score) evaluation approach was established and applied in comparative evaluation between RR GM and natural soybeans. Healthy male ICR mice during linear growth were selected; experimental mice were fed with 15% RR GM soybeans and 15% natural soybeans blending maize meal diets, and control mice were fed with single maize meal diet for 13 d; the mice were dissected after collecting blood samples and perfectly obtained nine organs or tissues to re- cord their masses and conduct statistical analyses. [Result] Plenty of matching information was obtained through simple design. The growth performance of treated mice was markedly of individual differences, some mice were thwarted due to regular intake of RR soybeans. Meanwhile, the functions and safety of RR soybeans were markedly lowered in overall nutritional and healthy effects than those of natural soybeans expressed in GS values, and presents some declines in nutrition and health of thymus, pancreas and spermary; especially, it can make thymus immune (P 〈0.05) in markedly lower level than that of natural soybeans. [ Conclusion] Therefore, major troubles and risks of RR soybeans intake are of personal risks in different degrees, in addition, it may increase sub-health and related chronic epi- demics risks, and herein it will presents certain safety issues. The creation of this novel evaluation system provides a simple and available evaluation approach for functions and potential risks revelation of food effects, and will yield far-reaching influences to safety evaluation and healthy development of GM foods, as well as public health.展开更多
本研究利用天然皂皮皂苷(Quillaja saponin,QS)与大豆分离蛋白(soybean protein isolate,SPI)稳定乳液凝胶的协同效应构建高蛋白、减脂的植物基蛋黄酱(plant-based mayonnaise,PM)。利用静态光散射、激光共聚焦、低形变流变学和质构特...本研究利用天然皂皮皂苷(Quillaja saponin,QS)与大豆分离蛋白(soybean protein isolate,SPI)稳定乳液凝胶的协同效应构建高蛋白、减脂的植物基蛋黄酱(plant-based mayonnaise,PM)。利用静态光散射、激光共聚焦、低形变流变学和质构特性对比分析PM与两种市售蛋黄酱在外观、乳滴粒径大小与分布、硬度、涂抹性、热稳定性和冻融稳定性等方面的特性。结果表明,以10%SPI和0.18%QS或10%SPI、0.18%QS和1%SPI凝胶颗粒(soy protein isolate gel particles,SGPs)为蛋白配料可构建出与商品蛋黄酱在外观、涂抹状态、质构以及流变特性上相媲美的高蛋白减脂PM(10%蛋白质与50%脂肪、11%蛋白质与40%脂肪,以质量分数计)。在该体系中,QS不仅赋予了高蛋白乳化制品黏度稀化的质构调节性,还赋予了SPI基乳液凝胶优越的热稳定性和冻融稳定性。本研究可为“高蛋白”“减脂”PM的开发与应用提供理论与技术指导。展开更多
文摘The soybean cultivar Tadang Muangpai was used to improve the productive quality of Thua-Nao and reduce the concentration of aflatoxin to less than 20 ppb. It was conducted at CMFCRC, Chiangmai, Thailand between Dec. 2006 and Mar. 2007. Soybean was boiled for 5 hours and then fermented at different time to create natural bacterial species, mainly Bacillus spp. Thua-Nao could be stored up to 90 days after in storage. Nutritional value, food value, and microorganisms content were investigated during fermentation and storage. Also, aflatoxin content of Thua-Nao was recorded during storage. The results showed that 3 days of soybean fermentation gave the best performance of Thua-Nao in term of nutritional value (protein = 47.12%), food value, and content of Bacillus spp. (2.78 × 10^9 CFU/g). Without being harmed from aflatoxin, Thua-Nao could be stored not more than 23 days in normal room (Tmax. = 33.9 ℃, Tmin. = 15.8 ℃) and not more than 36 days in climate-controlled room (Tmax. = 20 ℃, Tmin. = 15 ℃).
基金Swine Innovation Porc Canada provided the funding support
文摘Background: Soybean meal is an excellent and cost-effective protein source; however, its usage is limited in the piglet due to the presence of anti-nutritional factors and the antigens glycinin and β-conglycinin. The objective of the current study was to screen and select for bacteria that can be efficiently adopted to ferment soybean meal in order to solve this problem.Results: Bacteria were isolated from fermented soy foods and the grass carp intestine, and strains selected for high protease, cellulase and amylase activities. The isolated bacteria were characterized as Bacillus cereus, Bacillus subtilis and Bacilus amyloliquefacien, respectively. Fermentation with food-derived Isolate-2 and fish-derived F-9 increased crude protein content by 5.32% and 8.27%, respectively; improved the amino acid profile by increasing certain essential amino acids, broke down larger soy protein to 35 k Da and under, eliminated antigenicity against glycinin and β-conglycinin, and removed raffinose and stachyose in the soybean meal following a 24-h fermentation.Conclusions: Our results suggest these two B. amyloliquefaciens bacteria can efficiently solid state ferment soybean meal and ultimately produce a more utilizable food source for growing healthy piglets.
基金Supported by Development Fund of the Institute of Radiation Medicine(No.SF1227)Research Fund for Youth Scholars of Union Medical College(No.2012D03)Research Fund for the Doctoral Program of Higher Education of China(No.20121106120042)
文摘[Objective] This study aimed to evaluate tbe healthy risk of genetically modified ( GM ) soybeans by using a novel approach for functions and safety of food. [ Me^od] Different from traditional evaluation of substantial equivalence, three great innovations were performed in this study, involving in basic diet, evalu- ation approaches and principle, as well as the clarification of connotation differences between absolute and relative mass of organs. Hence a novel BDI-GS (Bendib Damage Index and General Score) evaluation approach was established and applied in comparative evaluation between RR GM and natural soybeans. Healthy male ICR mice during linear growth were selected; experimental mice were fed with 15% RR GM soybeans and 15% natural soybeans blending maize meal diets, and control mice were fed with single maize meal diet for 13 d; the mice were dissected after collecting blood samples and perfectly obtained nine organs or tissues to re- cord their masses and conduct statistical analyses. [Result] Plenty of matching information was obtained through simple design. The growth performance of treated mice was markedly of individual differences, some mice were thwarted due to regular intake of RR soybeans. Meanwhile, the functions and safety of RR soybeans were markedly lowered in overall nutritional and healthy effects than those of natural soybeans expressed in GS values, and presents some declines in nutrition and health of thymus, pancreas and spermary; especially, it can make thymus immune (P 〈0.05) in markedly lower level than that of natural soybeans. [ Conclusion] Therefore, major troubles and risks of RR soybeans intake are of personal risks in different degrees, in addition, it may increase sub-health and related chronic epi- demics risks, and herein it will presents certain safety issues. The creation of this novel evaluation system provides a simple and available evaluation approach for functions and potential risks revelation of food effects, and will yield far-reaching influences to safety evaluation and healthy development of GM foods, as well as public health.
文摘本研究利用天然皂皮皂苷(Quillaja saponin,QS)与大豆分离蛋白(soybean protein isolate,SPI)稳定乳液凝胶的协同效应构建高蛋白、减脂的植物基蛋黄酱(plant-based mayonnaise,PM)。利用静态光散射、激光共聚焦、低形变流变学和质构特性对比分析PM与两种市售蛋黄酱在外观、乳滴粒径大小与分布、硬度、涂抹性、热稳定性和冻融稳定性等方面的特性。结果表明,以10%SPI和0.18%QS或10%SPI、0.18%QS和1%SPI凝胶颗粒(soy protein isolate gel particles,SGPs)为蛋白配料可构建出与商品蛋黄酱在外观、涂抹状态、质构以及流变特性上相媲美的高蛋白减脂PM(10%蛋白质与50%脂肪、11%蛋白质与40%脂肪,以质量分数计)。在该体系中,QS不仅赋予了高蛋白乳化制品黏度稀化的质构调节性,还赋予了SPI基乳液凝胶优越的热稳定性和冻融稳定性。本研究可为“高蛋白”“减脂”PM的开发与应用提供理论与技术指导。