Drought stress is an abiotic stress that imposes serious constraints on plants. The present investigation was carried out to determine the inter-relationship between some physiological attributes of soybeans affected ...Drought stress is an abiotic stress that imposes serious constraints on plants. The present investigation was carried out to determine the inter-relationship between some physiological attributes of soybeans affected by drought stress and pure isolates ofAzotobacter andAzospirillum. Drought stress and bacterial application increased catalase and glutathione peroxidase activity, whereas drought stress increased superoxide dismutase activity during the pod-filling stage. Abscisic acid and proline levels increased due to drought stress and bacterial application during the flowering stage, whereas total plant nitrogen was enhanced under well-watered conditions when plants were inoculated with bacteria. The close relationship between enzyme activity and drought stress with bacteria indicated that antioxidant enzymes play an important role in alleviating the detrimental effects of water stress. In addition, the enhancement of abscisic acid and proline could be positively linked with drought stress, and drought-induced abscisic acid could induce proline accumulation and the expression of antioxidant enzyme genes.展开更多
A green house experiment was conducted at Dejen, Northwest Ethiopia, with the objective of quantifying the critical soil water deficit and P levels that affect yield and yield components of soybean, and determine the ...A green house experiment was conducted at Dejen, Northwest Ethiopia, with the objective of quantifying the critical soil water deficit and P levels that affect yield and yield components of soybean, and determine the critical soil water deficit levels influencing P uptake in soybean. The treatment consisted of factorial combination of four available soil water (ASW) deficit levels (0%, 25%, 50% and 75%) and four levels of phosphorus (0, 10, 20, and 30 kg·ha-1) laid out in RCBD with four replications using soy bean variety Jalale as a planting material. The experiment was conducted under green house condition at Dejen, South Ethiopia during the 2011 academic year. Air dried soil was filled in the pots and seeds were sown on May 13, 2011. Four plants were maintained on each pot after thinning till flowering but after flowering, the total number of plants per pot was reduced to three as one plant which was used for measurement of root biomass. The water deficit treatments were imposed after the plants have been fully established 2 weeks after emergence just before branching stage. The water deficiency was imposed through maintaining the soil moisture content below field capacity at the deficit levels of 25%, 50% and 75%. The 75% of ASW deficit resulted in the longest days (45) to flowering and maturity (99) compared to the 0%, 25% and 50% deficit levels. Also, the 75% of ASW deficit level resulted in shorter plants (55 cm), the lowest leaf area (82.6 cm2), the highest root to shoot ratio (0.0168) and the lowest DM accumulation (161.3 gm-2) compared to the other ASW deficit levels. Likewise, the 75% of ASW deficit level gave the lowest number of pods per plant (4.13), seeds per pod (1.69), 100 seed weight (2.54 g), seed yield (13.4 g·m-1), above ground biomass (174.6 g·plant-1) and harvest index (0.08) compared to the other ASW deficit treatments. The degree of sensitivity to drought increased dramatically (from 0.0423 at 25% to 0.9604 at 75%) with increase in water deficit level. Tissue analysis results indicated that the highest seed P concentration (1.285%) and uptake (432.5 g·plant-1) were obtained at the 0 ASW deficit and 30 kg·P·ha-1 and the lowest were obtained at 75% ASW deficit and all rate of applied P. On the contrary, the highest straw P concentration (1.88%) and uptake (552.7 g·plant-1) were recorded at 75% and 25% of ASW deficit levels and 30 kg·P·ha-1, respectively. However, the total P uptake was influenced only by ASW deficit levels in that the relatively minimum and maximum values were observed at 75% and 0% of ASW deficit levels, respectively. It can be concluded that the critical ASW deficit levels that affect yield and yield components of soybean and uptake of total P lie between 25% and 50% of available water deficit levels. The parameters started to decline significantly from the 50% of ASW deficit onwards. As it is a green house experiment, further study on more number of ASW deficit levels and soil types under different field conditions needs to be done to reach at a conclusive recommendation.展开更多
Soybean cultivar Bei 92-28 was tested in this experiment in 2000 to study the coupling effect of water and ferilizer on soybean yield.The results showed that the effect of irrigation varied among the levels of fertili...Soybean cultivar Bei 92-28 was tested in this experiment in 2000 to study the coupling effect of water and ferilizer on soybean yield.The results showed that the effect of irrigation varied among the levels of fertilizer application,and vice versa;pods per plant,seeds per pod.and 100-seed weight had positive correlations with soybean yield,but the degrees of correlations of different treatments were various;LAI and dry matter accumulation could be significantly increased when watered and applied fertilizer with different levels,but high fertilizer application treatment didn't obtain the highest yield;watering could increase the absolute absorption amount of N,P,K in seeds,but the accumulation rates were various.展开更多
Soybean Dongnong 47 was subjected to the experiments of increasing UV-B radiation and water stress on soybean yield components in different growth periods. The results showed that 100-seed weight greatly increased dur...Soybean Dongnong 47 was subjected to the experiments of increasing UV-B radiation and water stress on soybean yield components in different growth periods. The results showed that 100-seed weight greatly increased during the early stage of pod filling in the treatment of weak UV-B radiation, seed number per plant as well as seed weight per plant and Dongnong47 yield also increased, while the yield and yield components of Dongnong47 during the blossom to mature period were negatively affected in the treatment of intensive UV-B radiation. 100-seed weight of Dongnong47 all increased in the double factor treatments of UV-B radiation and water stress, with the drought intensified, seed number per plant, seed weight per plant and yield of Dongnong47 decreased, the change of 100-seed weight were various and the antagonistic action of UV-B radiation and water stress were related with their intensity.展开更多
Soybean (Glycine max (L.) Merrill) is one of the most important oil and protein sources in the world. Interactive effect of elevated carbon dioxide (CO2) and soil water availability potentially impact future food secu...Soybean (Glycine max (L.) Merrill) is one of the most important oil and protein sources in the world. Interactive effect of elevated carbon dioxide (CO2) and soil water availability potentially impact future food security of the world under climate change. A rhizotron growth chamber experiment was conducted to study soil moisture interactions with elevated CO2 on gaseous exchange parameters of soybean under two CO2 concentrations (380 and 800 μmol·mol-1) with three soil moisture levels. Elevated CO2 decreased photosynthetic rate (11.1% and 10.8%), stomatal conductance (40.5% and 36.0%), intercellular CO2 concentration (16.68% and 12.28%), relative intercellular CO2 concentration (17.4% and 11.2%), and transpiration rate (43.6% and 39%) at 42 and 47 DAP. This down-regulation of photosynthesis was probably caused by low leaf nitrogen content and decrease in uptake of nutrients due to decrease in stomatal conductance and transpiration rate. Water use efficiency (WUE) increased under elevated CO2 because increase in total dry weight of plant was greater than that of water use under high CO2 conditions. Plants under normal and high soil moisture levels had significantly higher photosynthetic rate (7% to 16%) favored by optimum soil moisture content and high specific water content of soybean plants. Total dry matter production was significantly high when plants grown under elevated CO2 with normal (74.3% to 137.3%) soil moisture level. Photosynthetic rate was significantly and positively correlated with leaf conductance and intercellular CO2 concentration but WUE was significantly negatively correlated with leaf conductance, intercellular CO2 concentration and transpiration rate. However, the effect of high CO2 on plants depends on availability of nutrients and soil moisture for positive feedback from CO2 enrichment.展开更多
The purpose of this study was to investigate the physiological responses of drought-tolerant and drought-susceptible soybean genotypes to exogenous abscisic acid(ABA) application during progressive soil drying at seed...The purpose of this study was to investigate the physiological responses of drought-tolerant and drought-susceptible soybean genotypes to exogenous abscisic acid(ABA) application during progressive soil drying at seedling stages. Five-day old soybean seedlings were transplanted into PVC tubes filled with soil mixture. Seedlings were watered daily with similar water volumes until second trifoliate leaves emerged, and thereafter soil drying with or without exogenous ABA application was imposed. Half of the seedlings of each genotype were left for regular watering as control plants. Soil water status declined significantly over seven days of withholding water supply for both genotypes. Leaf expansion rate, stomatal conductance(g_s), leaf water potential(ψ_w), and relative water content of leaves(%RWC) declined significantly under soil drying as well as soil drying with ABA application, compared to their values for well-watered soybean genotypes. However, a drought-tolerant genotype(C12) responded more rapidly than a drought-susceptible genotype(C08) after imposition of soil drying and soil drying with exogenous ABA. In addition, application of exogenous ABA to water-restricted soybeans resulted in higher %RWC and ψwin the drought-tolerant than in the drought-susceptible genotype. Compared to the drought-susceptible genotype, the drought-tolerant genotype was more responsive to exogenous ABA application, resulting in a higher root-to-shoot ratio.展开更多
Nowadays more than 70% of the fresh water available worldwide is used for agriculture. In Argentina, extensive crops are not usually irrigated, so the cropping production depends mostly on rainfall water. In order to ...Nowadays more than 70% of the fresh water available worldwide is used for agriculture. In Argentina, extensive crops are not usually irrigated, so the cropping production depends mostly on rainfall water. In order to know how many liters of water are needed to produce a ton of soybeans, wheat and maize in the Pergamino district, Province of Buenos Aires, its Water Footprint was estimated by the Hoekstra method. Evaporation and rainfall data was obtained from SIGA INTA platform and production data was obtained from the Ministry of Agriculture. The results indicated that the average annual total water footprint values for soybean, corn and wheat crops for the period 2013-2018 in the Pergamino district, province of Buenos Aires, are 1,388 l<span style="white-space:nowrap;">∙</span>kg<sup><span style="white-space:nowrap;">−</span>1</sup>, 693 l<span style="white-space:nowrap;">∙</span>kg<sup><span style="white-space:nowrap;">−</span>1</sup> and 1,249 l<span style="white-space:nowrap;">∙</span>kg<sup><span style="white-space:nowrap;">−</span>1</sup> respectively. These were lower than the global average reference values. The obtained results allowed future analysis advancing in the knowledge of the use of water productivity in grain production.展开更多
Soybean hulls, an abundant byproduct of soybean processing, contain rich phytochemicals, fibers, proteins, and minerals. Currently soybean hulls are primarily used as animal feeds. For value-added soybean hull utiliza...Soybean hulls, an abundant byproduct of soybean processing, contain rich phytochemicals, fibers, proteins, and minerals. Currently soybean hulls are primarily used as animal feeds. For value-added soybean hull utilization, 25% soybean hulls were substituted for amaranth or whole oat flour (WOF) in novel gluten-free cookies. Composition, nutritional values, water-holding capacities, correlation between properties, and pasting and rheological properties of soybean hulls, amaranth, and WOF were appraised in comparison to wheat flour. Water loss, cookie texture, and geometrical properties of the cookies were examined. The results disclosed that soybean hulls, amaranth and WOF contain higher protein content, minerals, fiber, special amino acids, and critical vitamins (C and K) than wheat flour. Considerably higher total amino acid content was found in soybean hulls (18.33%) than wheat flour (12.77%). Water-holding capacities increased by replacing amaranth and WOF with soybean hulls. Soybean hulls exhibited higher rheological elastic properties than amaranth, WOF and wheat flours. The soybean hulls utilized in amaranth or WOF cookies greatly improved their nutritional value, the water retention and moisture content along with acceptable physical properties when compared to wheat flour cookies. This study explored the feasibility and potential of utilizing soybean hulls with amaranth and WOF in gluten-free bakery products and other food applications.展开更多
Two field experiments were carried out during growing seasons 2010 and 2011, it executed in research farm of national research center in Nubaryia region, Egypt to study the effect of pulse drip irrigation and mulching...Two field experiments were carried out during growing seasons 2010 and 2011, it executed in research farm of national research center in Nubaryia region, Egypt to study the effect of pulse drip irrigation and mulching systems for saving water, increasing and improving yield of soybean. The study factors were, pulse drip irrigation technology (adding of daily water requirements on 4 times, 8 times, 12 times compared with adding of daily water requirements on 1 time) and mulching systems (covering the soil with black plastic mulch “BPM”, rice straw mulch “RSM” and the control treatment was soil surface without mulch “WM”). The following parameters were studied to evaluate the effect of pulse drip irrigation and mulching systems: 1) Soil moisture distribution in root zone, 2) Growth characters of soybean plant, 3) Yield of soybean, 4) Irrigation water use efficiency of soybean “IWUE soybean”, and 5) Oil content and oil yield, 6) Protein content and protein yield, 7) Economical parameter. According to the economical view and the results of statistical analysis for effect of pulse drip irrigation and mulching systems on yield, quality traits and IWUE soybean indicated that, applying the irrigation requirements on 8 pulses/day with using BPM is the best conditions because under these conditions was occurred the highest value of yield, quality traits and IWUE soybean and there was significant deference between this case and other treatments. Where, pulse irrigation technique increase from water movement in horizontal direction than vertical direction hence improve from soil moisture distribution and wetted soil volume in root zone and using BPM decrease from evaporation process rate from soil surface hence decreasing of salts accumulation in addition to decreasing of weed growth in the root zone. All traits at AIR on 12 pulses/ day are decreased by increasing of pulses, this may be due to irrigation water was very small with every pulse at AIR on 12 pulses/day in addition increasing the total time of time-off, this mean, un-sufficient application for irrigation water to remove water stress in the root zone.展开更多
文摘Drought stress is an abiotic stress that imposes serious constraints on plants. The present investigation was carried out to determine the inter-relationship between some physiological attributes of soybeans affected by drought stress and pure isolates ofAzotobacter andAzospirillum. Drought stress and bacterial application increased catalase and glutathione peroxidase activity, whereas drought stress increased superoxide dismutase activity during the pod-filling stage. Abscisic acid and proline levels increased due to drought stress and bacterial application during the flowering stage, whereas total plant nitrogen was enhanced under well-watered conditions when plants were inoculated with bacteria. The close relationship between enzyme activity and drought stress with bacteria indicated that antioxidant enzymes play an important role in alleviating the detrimental effects of water stress. In addition, the enhancement of abscisic acid and proline could be positively linked with drought stress, and drought-induced abscisic acid could induce proline accumulation and the expression of antioxidant enzyme genes.
文摘A green house experiment was conducted at Dejen, Northwest Ethiopia, with the objective of quantifying the critical soil water deficit and P levels that affect yield and yield components of soybean, and determine the critical soil water deficit levels influencing P uptake in soybean. The treatment consisted of factorial combination of four available soil water (ASW) deficit levels (0%, 25%, 50% and 75%) and four levels of phosphorus (0, 10, 20, and 30 kg·ha-1) laid out in RCBD with four replications using soy bean variety Jalale as a planting material. The experiment was conducted under green house condition at Dejen, South Ethiopia during the 2011 academic year. Air dried soil was filled in the pots and seeds were sown on May 13, 2011. Four plants were maintained on each pot after thinning till flowering but after flowering, the total number of plants per pot was reduced to three as one plant which was used for measurement of root biomass. The water deficit treatments were imposed after the plants have been fully established 2 weeks after emergence just before branching stage. The water deficiency was imposed through maintaining the soil moisture content below field capacity at the deficit levels of 25%, 50% and 75%. The 75% of ASW deficit resulted in the longest days (45) to flowering and maturity (99) compared to the 0%, 25% and 50% deficit levels. Also, the 75% of ASW deficit level resulted in shorter plants (55 cm), the lowest leaf area (82.6 cm2), the highest root to shoot ratio (0.0168) and the lowest DM accumulation (161.3 gm-2) compared to the other ASW deficit levels. Likewise, the 75% of ASW deficit level gave the lowest number of pods per plant (4.13), seeds per pod (1.69), 100 seed weight (2.54 g), seed yield (13.4 g·m-1), above ground biomass (174.6 g·plant-1) and harvest index (0.08) compared to the other ASW deficit treatments. The degree of sensitivity to drought increased dramatically (from 0.0423 at 25% to 0.9604 at 75%) with increase in water deficit level. Tissue analysis results indicated that the highest seed P concentration (1.285%) and uptake (432.5 g·plant-1) were obtained at the 0 ASW deficit and 30 kg·P·ha-1 and the lowest were obtained at 75% ASW deficit and all rate of applied P. On the contrary, the highest straw P concentration (1.88%) and uptake (552.7 g·plant-1) were recorded at 75% and 25% of ASW deficit levels and 30 kg·P·ha-1, respectively. However, the total P uptake was influenced only by ASW deficit levels in that the relatively minimum and maximum values were observed at 75% and 0% of ASW deficit levels, respectively. It can be concluded that the critical ASW deficit levels that affect yield and yield components of soybean and uptake of total P lie between 25% and 50% of available water deficit levels. The parameters started to decline significantly from the 50% of ASW deficit onwards. As it is a green house experiment, further study on more number of ASW deficit levels and soil types under different field conditions needs to be done to reach at a conclusive recommendation.
文摘Soybean cultivar Bei 92-28 was tested in this experiment in 2000 to study the coupling effect of water and ferilizer on soybean yield.The results showed that the effect of irrigation varied among the levels of fertilizer application,and vice versa;pods per plant,seeds per pod.and 100-seed weight had positive correlations with soybean yield,but the degrees of correlations of different treatments were various;LAI and dry matter accumulation could be significantly increased when watered and applied fertilizer with different levels,but high fertilizer application treatment didn't obtain the highest yield;watering could increase the absolute absorption amount of N,P,K in seeds,but the accumulation rates were various.
基金Supported by Postdoctoral Fund of Settling Down in Heilongjiang Province (LBH-Z05086)Scientific Research Fund of Northeast Agricultural University
文摘Soybean Dongnong 47 was subjected to the experiments of increasing UV-B radiation and water stress on soybean yield components in different growth periods. The results showed that 100-seed weight greatly increased during the early stage of pod filling in the treatment of weak UV-B radiation, seed number per plant as well as seed weight per plant and Dongnong47 yield also increased, while the yield and yield components of Dongnong47 during the blossom to mature period were negatively affected in the treatment of intensive UV-B radiation. 100-seed weight of Dongnong47 all increased in the double factor treatments of UV-B radiation and water stress, with the drought intensified, seed number per plant, seed weight per plant and yield of Dongnong47 decreased, the change of 100-seed weight were various and the antagonistic action of UV-B radiation and water stress were related with their intensity.
文摘Soybean (Glycine max (L.) Merrill) is one of the most important oil and protein sources in the world. Interactive effect of elevated carbon dioxide (CO2) and soil water availability potentially impact future food security of the world under climate change. A rhizotron growth chamber experiment was conducted to study soil moisture interactions with elevated CO2 on gaseous exchange parameters of soybean under two CO2 concentrations (380 and 800 μmol·mol-1) with three soil moisture levels. Elevated CO2 decreased photosynthetic rate (11.1% and 10.8%), stomatal conductance (40.5% and 36.0%), intercellular CO2 concentration (16.68% and 12.28%), relative intercellular CO2 concentration (17.4% and 11.2%), and transpiration rate (43.6% and 39%) at 42 and 47 DAP. This down-regulation of photosynthesis was probably caused by low leaf nitrogen content and decrease in uptake of nutrients due to decrease in stomatal conductance and transpiration rate. Water use efficiency (WUE) increased under elevated CO2 because increase in total dry weight of plant was greater than that of water use under high CO2 conditions. Plants under normal and high soil moisture levels had significantly higher photosynthetic rate (7% to 16%) favored by optimum soil moisture content and high specific water content of soybean plants. Total dry matter production was significantly high when plants grown under elevated CO2 with normal (74.3% to 137.3%) soil moisture level. Photosynthetic rate was significantly and positively correlated with leaf conductance and intercellular CO2 concentration but WUE was significantly negatively correlated with leaf conductance, intercellular CO2 concentration and transpiration rate. However, the effect of high CO2 on plants depends on availability of nutrients and soil moisture for positive feedback from CO2 enrichment.
基金supported by the Hong Kong RGC Collaborative Research Fund(CUHK3/CRF/11G)to Prof.Hon-Ming Lam and Prof.Jianhua Zhang
文摘The purpose of this study was to investigate the physiological responses of drought-tolerant and drought-susceptible soybean genotypes to exogenous abscisic acid(ABA) application during progressive soil drying at seedling stages. Five-day old soybean seedlings were transplanted into PVC tubes filled with soil mixture. Seedlings were watered daily with similar water volumes until second trifoliate leaves emerged, and thereafter soil drying with or without exogenous ABA application was imposed. Half of the seedlings of each genotype were left for regular watering as control plants. Soil water status declined significantly over seven days of withholding water supply for both genotypes. Leaf expansion rate, stomatal conductance(g_s), leaf water potential(ψ_w), and relative water content of leaves(%RWC) declined significantly under soil drying as well as soil drying with ABA application, compared to their values for well-watered soybean genotypes. However, a drought-tolerant genotype(C12) responded more rapidly than a drought-susceptible genotype(C08) after imposition of soil drying and soil drying with exogenous ABA. In addition, application of exogenous ABA to water-restricted soybeans resulted in higher %RWC and ψwin the drought-tolerant than in the drought-susceptible genotype. Compared to the drought-susceptible genotype, the drought-tolerant genotype was more responsive to exogenous ABA application, resulting in a higher root-to-shoot ratio.
文摘Nowadays more than 70% of the fresh water available worldwide is used for agriculture. In Argentina, extensive crops are not usually irrigated, so the cropping production depends mostly on rainfall water. In order to know how many liters of water are needed to produce a ton of soybeans, wheat and maize in the Pergamino district, Province of Buenos Aires, its Water Footprint was estimated by the Hoekstra method. Evaporation and rainfall data was obtained from SIGA INTA platform and production data was obtained from the Ministry of Agriculture. The results indicated that the average annual total water footprint values for soybean, corn and wheat crops for the period 2013-2018 in the Pergamino district, province of Buenos Aires, are 1,388 l<span style="white-space:nowrap;">∙</span>kg<sup><span style="white-space:nowrap;">−</span>1</sup>, 693 l<span style="white-space:nowrap;">∙</span>kg<sup><span style="white-space:nowrap;">−</span>1</sup> and 1,249 l<span style="white-space:nowrap;">∙</span>kg<sup><span style="white-space:nowrap;">−</span>1</sup> respectively. These were lower than the global average reference values. The obtained results allowed future analysis advancing in the knowledge of the use of water productivity in grain production.
文摘Soybean hulls, an abundant byproduct of soybean processing, contain rich phytochemicals, fibers, proteins, and minerals. Currently soybean hulls are primarily used as animal feeds. For value-added soybean hull utilization, 25% soybean hulls were substituted for amaranth or whole oat flour (WOF) in novel gluten-free cookies. Composition, nutritional values, water-holding capacities, correlation between properties, and pasting and rheological properties of soybean hulls, amaranth, and WOF were appraised in comparison to wheat flour. Water loss, cookie texture, and geometrical properties of the cookies were examined. The results disclosed that soybean hulls, amaranth and WOF contain higher protein content, minerals, fiber, special amino acids, and critical vitamins (C and K) than wheat flour. Considerably higher total amino acid content was found in soybean hulls (18.33%) than wheat flour (12.77%). Water-holding capacities increased by replacing amaranth and WOF with soybean hulls. Soybean hulls exhibited higher rheological elastic properties than amaranth, WOF and wheat flours. The soybean hulls utilized in amaranth or WOF cookies greatly improved their nutritional value, the water retention and moisture content along with acceptable physical properties when compared to wheat flour cookies. This study explored the feasibility and potential of utilizing soybean hulls with amaranth and WOF in gluten-free bakery products and other food applications.
文摘Two field experiments were carried out during growing seasons 2010 and 2011, it executed in research farm of national research center in Nubaryia region, Egypt to study the effect of pulse drip irrigation and mulching systems for saving water, increasing and improving yield of soybean. The study factors were, pulse drip irrigation technology (adding of daily water requirements on 4 times, 8 times, 12 times compared with adding of daily water requirements on 1 time) and mulching systems (covering the soil with black plastic mulch “BPM”, rice straw mulch “RSM” and the control treatment was soil surface without mulch “WM”). The following parameters were studied to evaluate the effect of pulse drip irrigation and mulching systems: 1) Soil moisture distribution in root zone, 2) Growth characters of soybean plant, 3) Yield of soybean, 4) Irrigation water use efficiency of soybean “IWUE soybean”, and 5) Oil content and oil yield, 6) Protein content and protein yield, 7) Economical parameter. According to the economical view and the results of statistical analysis for effect of pulse drip irrigation and mulching systems on yield, quality traits and IWUE soybean indicated that, applying the irrigation requirements on 8 pulses/day with using BPM is the best conditions because under these conditions was occurred the highest value of yield, quality traits and IWUE soybean and there was significant deference between this case and other treatments. Where, pulse irrigation technique increase from water movement in horizontal direction than vertical direction hence improve from soil moisture distribution and wetted soil volume in root zone and using BPM decrease from evaporation process rate from soil surface hence decreasing of salts accumulation in addition to decreasing of weed growth in the root zone. All traits at AIR on 12 pulses/ day are decreased by increasing of pulses, this may be due to irrigation water was very small with every pulse at AIR on 12 pulses/day in addition increasing the total time of time-off, this mean, un-sufficient application for irrigation water to remove water stress in the root zone.