A new phenol-degrading bacterium with high biodegradation activity and high tolerance of phenol, strain PD 12, was isolated from the activated sludge of Tianjin Jizhuangzi Wastewater Treatment Facility in China. This ...A new phenol-degrading bacterium with high biodegradation activity and high tolerance of phenol, strain PD 12, was isolated from the activated sludge of Tianjin Jizhuangzi Wastewater Treatment Facility in China. This strain was capable of removing 500 mg phenol/L in liquid minimal medium by 99.6% within 9 h and metabolizing phenol at concentrations up to 1100 mg/L. DNA sequencing and homologous analysis of 16S rRNA gene identified PD12 to be an Acinetobacter sp. Polyvinyl alcohol （PVA） was used as a gel matrix to immobilize Acinetobacter sp. strain PDI2 by repeated freezing and thawing. The factors affecting phenol degradation of immobilized cells were investigated, and the results showed that the immobilized cells could tolerate a high phenol level and protected the bacteria against changes in temperature and pH. Storage stability and reusability tests revealed that the phenol degradation functions of immobilized cells were stable after reuse for 50 times or storing at 4℃ for 50 d. These results indicate that immobilized Acinetobacter sp. strain PD 12 possesses a good application potential in the treatment of phenol-containing wastewater.展开更多
A new species of small tree frog from a primary montane tropical forest of central Vietnam, Tay Nguyen Plateau, is described based on morphological, molecular, and acoustic evidence. The Golden Bug-Eyed Frog, Theloder...A new species of small tree frog from a primary montane tropical forest of central Vietnam, Tay Nguyen Plateau, is described based on morphological, molecular, and acoustic evidence. The Golden Bug-Eyed Frog, Theloderma auratum sp. nov., is distinguishable from its congeners and other small rhacophorid species based on a combination of the following morphological attributes： （1） bony ridges on head absent; （2） smooth skin completely lacking calcified warts or asperities; （3） pointed elongated tapering snout; （4） vocal opening in males absent; （5） vomerine teeth absent; （6） males of small body size （SVL 21.8-26.4 mm）; （7） head longer than wide; ED/SVL ratio 13%-15%; ESL/SVL ratio 16%-20%; （8） small tympanum （TD/EL ratio 50%-60%） with few tiny tubercles; （9） supratympanic fold absent; （10） ventral surfaces completely smooth; （11 ） webbing between fingers absent; （12） outer and inner metacarpal tubercles present, supernumerary metacarpal tubercle single, medial, oval in shape; （13） toes half-webbed： I 2-21/4 II 11/^-23/~ III 2-31/4 IV 3-11/2 V; （14） inner metatarsal tubercle present, oval; outer metatarsal tubercle absent; （15） iris bicolored; （16） dorsal surfaces golden-yellow with sparse golden-orange speckling or reticulations and few small dark-brown spots; （17） lateral sides of head and body with wide dark reddish-brown to black lateral stripes, clearlyseparated from lighter dorsal coloration by straight contrasting edge; （18） ventral surfaces of body, throat, and chest greyish-blue with indistinct brown confluent blotches; （19） upper eyelids with few （3-5） very small flat reddish superciliary tubercles; （20） limbs dorsally reddish-brown, ventrally brown with small bluish-white speckles. The new species is also distinct from all congeners in 12S rRNA to 16S rRNA mitochondrial DNA fragment sequences （uncorrected genetic distance P〉8.9%）. Advertisement call and tadpole morphology of the new species are described. Our molecular data showed Theloderma auratum sp. nov. to be a sister species of Th. palliatum from Langbian Plateau in southern Vietnam.展开更多
基金Project supported by the Undergraduate Research Foundation of Nankai University (2004).
文摘A new phenol-degrading bacterium with high biodegradation activity and high tolerance of phenol, strain PD 12, was isolated from the activated sludge of Tianjin Jizhuangzi Wastewater Treatment Facility in China. This strain was capable of removing 500 mg phenol/L in liquid minimal medium by 99.6% within 9 h and metabolizing phenol at concentrations up to 1100 mg/L. DNA sequencing and homologous analysis of 16S rRNA gene identified PD12 to be an Acinetobacter sp. Polyvinyl alcohol （PVA） was used as a gel matrix to immobilize Acinetobacter sp. strain PDI2 by repeated freezing and thawing. The factors affecting phenol degradation of immobilized cells were investigated, and the results showed that the immobilized cells could tolerate a high phenol level and protected the bacteria against changes in temperature and pH. Storage stability and reusability tests revealed that the phenol degradation functions of immobilized cells were stable after reuse for 50 times or storing at 4℃ for 50 d. These results indicate that immobilized Acinetobacter sp. strain PD 12 possesses a good application potential in the treatment of phenol-containing wastewater.
基金partially supported by the Russian Foundation of Basic Research(RFBR 15-29-02771RFBR17-54-54002_viet_a)molecular analyses,preservation,and storage of specimens were carried out with financial support from the Russian Science Foundation(RSF 14-50-00029)
文摘A new species of small tree frog from a primary montane tropical forest of central Vietnam, Tay Nguyen Plateau, is described based on morphological, molecular, and acoustic evidence. The Golden Bug-Eyed Frog, Theloderma auratum sp. nov., is distinguishable from its congeners and other small rhacophorid species based on a combination of the following morphological attributes： （1） bony ridges on head absent; （2） smooth skin completely lacking calcified warts or asperities; （3） pointed elongated tapering snout; （4） vocal opening in males absent; （5） vomerine teeth absent; （6） males of small body size （SVL 21.8-26.4 mm）; （7） head longer than wide; ED/SVL ratio 13%-15%; ESL/SVL ratio 16%-20%; （8） small tympanum （TD/EL ratio 50%-60%） with few tiny tubercles; （9） supratympanic fold absent; （10） ventral surfaces completely smooth; （11 ） webbing between fingers absent; （12） outer and inner metacarpal tubercles present, supernumerary metacarpal tubercle single, medial, oval in shape; （13） toes half-webbed： I 2-21/4 II 11/^-23/~ III 2-31/4 IV 3-11/2 V; （14） inner metatarsal tubercle present, oval; outer metatarsal tubercle absent; （15） iris bicolored; （16） dorsal surfaces golden-yellow with sparse golden-orange speckling or reticulations and few small dark-brown spots; （17） lateral sides of head and body with wide dark reddish-brown to black lateral stripes, clearlyseparated from lighter dorsal coloration by straight contrasting edge; （18） ventral surfaces of body, throat, and chest greyish-blue with indistinct brown confluent blotches; （19） upper eyelids with few （3-5） very small flat reddish superciliary tubercles; （20） limbs dorsally reddish-brown, ventrally brown with small bluish-white speckles. The new species is also distinct from all congeners in 12S rRNA to 16S rRNA mitochondrial DNA fragment sequences （uncorrected genetic distance P〉8.9%）. Advertisement call and tadpole morphology of the new species are described. Our molecular data showed Theloderma auratum sp. nov. to be a sister species of Th. palliatum from Langbian Plateau in southern Vietnam.
