Biofilm forming bacteria are omnipresent in the marine environment.Pseudoalteromonas is one of the largest within the γ-proteobacteria class,and a member of marine bacteria.Species of Pseudoalteromonas are generally ...Biofilm forming bacteria are omnipresent in the marine environment.Pseudoalteromonas is one of the largest within the γ-proteobacteria class,and a member of marine bacteria.Species of Pseudoalteromonas are generally found in association with marine eukaryotes.In this work,we present the isolation and characterization of two strains forming biofilm on rock surface and associated with marine sponge.They were identified using 16SrDNA as Pseudoalteromonas prydzensis alex,and Pseudoalteromonas sp.alex.They showed the highest titer in biofilm formation quantified using the test tube method using crystal violet.展开更多
Surfaces submerged in seawater are colonized by various microorganisms,resulting in the formation of heterogenic marine biofilms.This work aims to evaluate the biofilm formation by Cobetia marina alex and doing a comp...Surfaces submerged in seawater are colonized by various microorganisms,resulting in the formation of heterogenic marine biofilms.This work aims to evaluate the biofilm formation by Cobetia marina alex and doing a comparative study between this promising strain with the two bacterial strains isolated previously from the Mediterranean seawater,Alexandria,Egypt.Three strains;Cobetia marina alex,Pseudoalteromonas sp.alex,and Pseudoalteromonas prydzensis alex were screened for biofilm formation using the crystal violet(CV)quantification method in a single culture.The values of biofilm formed were OD600=3.0,2.7,and 2.6,respectively leading to their selection for further evaluation.However,factors affecting biofilm formation by C.marina alex were investigated.Biofilm formation was evaluated in single and multispecies consortia.Synergistic and antagonistic interactions proved in this work lead to the belief that these bacteria have the capability to produce some interesting signal molecules N-acyl Homoserine Lactones(AHLs).展开更多
文摘Biofilm forming bacteria are omnipresent in the marine environment.Pseudoalteromonas is one of the largest within the γ-proteobacteria class,and a member of marine bacteria.Species of Pseudoalteromonas are generally found in association with marine eukaryotes.In this work,we present the isolation and characterization of two strains forming biofilm on rock surface and associated with marine sponge.They were identified using 16SrDNA as Pseudoalteromonas prydzensis alex,and Pseudoalteromonas sp.alex.They showed the highest titer in biofilm formation quantified using the test tube method using crystal violet.
文摘Surfaces submerged in seawater are colonized by various microorganisms,resulting in the formation of heterogenic marine biofilms.This work aims to evaluate the biofilm formation by Cobetia marina alex and doing a comparative study between this promising strain with the two bacterial strains isolated previously from the Mediterranean seawater,Alexandria,Egypt.Three strains;Cobetia marina alex,Pseudoalteromonas sp.alex,and Pseudoalteromonas prydzensis alex were screened for biofilm formation using the crystal violet(CV)quantification method in a single culture.The values of biofilm formed were OD600=3.0,2.7,and 2.6,respectively leading to their selection for further evaluation.However,factors affecting biofilm formation by C.marina alex were investigated.Biofilm formation was evaluated in single and multispecies consortia.Synergistic and antagonistic interactions proved in this work lead to the belief that these bacteria have the capability to produce some interesting signal molecules N-acyl Homoserine Lactones(AHLs).