Western blot analysis revealed that one IgG<sub>1</sub> monoclonal antibody （mAb） to sp18 family membrane proteins （Mr. 14, 16 and 18 ku） of bovine sperm reacted faintly with protein bands of 14,18, 22...Western blot analysis revealed that one IgG<sub>1</sub> monoclonal antibody （mAb） to sp18 family membrane proteins （Mr. 14, 16 and 18 ku） of bovine sperm reacted faintly with protein bands of 14,18, 22, 30 and 60 ku （reducing） in samples of mouse sperm. The mAb also reacted to protein of egg lysozyme. Using a laser confocal microscope, indirect immunofluorescence （IIF） showed that the sp18 antigens were present in the posterior head of murine sperm. In murine in vitro fertilization （IVF） and embryo development trails, a total of 426 oocytes from C<sub>57</sub>BL/6 and F<sub>1</sub> hybrid strain (CD<sub>1</sub>×C<sub>57</sub>BL/6 cross) of 12, female mice were used in 3 independent trails. After preincubating capacitated sperm with 182 μg/mL of sp18 mAb in the modified TYH IVF medium for 15—20 min, cumulus-oocyte-complexes were introduced. The fertilization rate in sp18 mAb groups was 77.1%, which was not significantly （P】0.05） different from the nonspecific mouse IgG（79.2 %） and non-IgG（80.3%） control groups. Fertilized oocytes had been展开更多
文摘Western blot analysis revealed that one IgG<sub>1</sub> monoclonal antibody （mAb） to sp18 family membrane proteins （Mr. 14, 16 and 18 ku） of bovine sperm reacted faintly with protein bands of 14,18, 22, 30 and 60 ku （reducing） in samples of mouse sperm. The mAb also reacted to protein of egg lysozyme. Using a laser confocal microscope, indirect immunofluorescence （IIF） showed that the sp18 antigens were present in the posterior head of murine sperm. In murine in vitro fertilization （IVF） and embryo development trails, a total of 426 oocytes from C<sub>57</sub>BL/6 and F<sub>1</sub> hybrid strain (CD<sub>1</sub>×C<sub>57</sub>BL/6 cross) of 12, female mice were used in 3 independent trails. After preincubating capacitated sperm with 182 μg/mL of sp18 mAb in the modified TYH IVF medium for 15—20 min, cumulus-oocyte-complexes were introduced. The fertilization rate in sp18 mAb groups was 77.1%, which was not significantly （P】0.05） different from the nonspecific mouse IgG（79.2 %） and non-IgG（80.3%） control groups. Fertilized oocytes had been
