目的:采用color-pilot颜色校正软件校正比色片数码照片并分析其在色度空间中的位置变化。方法:采用Vita 3D Master比色板作为试件,在诊室内拍摄比色片数码照片,并运用color-pilot颜色校正软件进行校正,最后与暗箱内拍摄的相应比色片数...目的:采用color-pilot颜色校正软件校正比色片数码照片并分析其在色度空间中的位置变化。方法:采用Vita 3D Master比色板作为试件,在诊室内拍摄比色片数码照片,并运用color-pilot颜色校正软件进行校正,最后与暗箱内拍摄的相应比色片数码照片进行对比。结果:比较诊室内与暗箱内拍摄的比色片数码照片,发现两组照片L*值之间、a*值之间差异明显(P<0.05),b*值之间则无明显差异(P>0.05);运用color-pilot颜色校正软件校正诊室内拍摄的数码照片后,发现两组照片L*值之间的差异仍然存在(P<0.05),但差异缩小(较诊室内拍摄的数码照片,校正后的数码照片L*值更接近于暗箱内拍摄的数码照片L*值),而a*值之间无明显差异(P>0.05),b*值之间依然无明显差异(P>0.05)。二者在色度空间中的位置也较校正前更为靠近。计算暗箱内、诊室内、校正后3组照片的ΔE值发现,校正后与暗箱内数码照片此值最小。结论:color-pilot颜色校正软件校正比色片数码照片后,可以在一定程度上减小数码照片的色彩偏差。展开更多
This study aimed to identify the optimal neural progenitor cell transplantation time for spinal cord injury in rats via the subarachnoid space. Cultured neural progenitor cells from 14-day embryonic rats, constitutive...This study aimed to identify the optimal neural progenitor cell transplantation time for spinal cord injury in rats via the subarachnoid space. Cultured neural progenitor cells from 14-day embryonic rats, constitutively expressing enhanced green fluorescence protein, or media alone, were injected into the subarachnoid space of adult rats at 1 hour （acute stage）, 7 days （subacute stage） and 28 days （chronic stage） after contusive spinal cord injury. Results showed that grafted neural progenitor cells migrated and aggregated around the blood vessels of the injured region, and infiltrated the spinal cord parenchyma along the tissue spaces in the acute stage transplantation group. However, this was not observed in subacute and chronic stage transplantation groups. 04- and glial fibrillary acidic protein-positive cells, representing oligodendrocytes and astrocytes respectively, were detected in the core of the grafted cluster attached to the cauda equina pia surface in the chronic stage transplantation group 8 weeks after transplantation. Both acute and subacute stage transplantation groups were negative for 04 and glial fibrillary acidic protein cells. Basso, Beattie and Bresnahan scale score comparisons indicated that rat hind limb locomotor activity showed better recovery after acute stage transplantation than after subacute and chronic transplantation. Our experimental findings suggest that the subarachnoid route could be useful for transplantation of neural progenitor cells at the acute stage of spinal cord injury. Although grafted cells survived only for a short time and did not differentiate into astrocytes or neurons, they were able to reach the parenchyma of the injured spinal cord and improve neurological function in rats. Transplantation efficacy was enhanced at the acute stage in comparison with subacute and chronic stages.展开更多
文摘目的:采用color-pilot颜色校正软件校正比色片数码照片并分析其在色度空间中的位置变化。方法:采用Vita 3D Master比色板作为试件,在诊室内拍摄比色片数码照片,并运用color-pilot颜色校正软件进行校正,最后与暗箱内拍摄的相应比色片数码照片进行对比。结果:比较诊室内与暗箱内拍摄的比色片数码照片,发现两组照片L*值之间、a*值之间差异明显(P<0.05),b*值之间则无明显差异(P>0.05);运用color-pilot颜色校正软件校正诊室内拍摄的数码照片后,发现两组照片L*值之间的差异仍然存在(P<0.05),但差异缩小(较诊室内拍摄的数码照片,校正后的数码照片L*值更接近于暗箱内拍摄的数码照片L*值),而a*值之间无明显差异(P>0.05),b*值之间依然无明显差异(P>0.05)。二者在色度空间中的位置也较校正前更为靠近。计算暗箱内、诊室内、校正后3组照片的ΔE值发现,校正后与暗箱内数码照片此值最小。结论:color-pilot颜色校正软件校正比色片数码照片后,可以在一定程度上减小数码照片的色彩偏差。
基金supported by the People’s Liberation Army Fund for Special Projects of Medical Sciences, No. 06G114
文摘This study aimed to identify the optimal neural progenitor cell transplantation time for spinal cord injury in rats via the subarachnoid space. Cultured neural progenitor cells from 14-day embryonic rats, constitutively expressing enhanced green fluorescence protein, or media alone, were injected into the subarachnoid space of adult rats at 1 hour （acute stage）, 7 days （subacute stage） and 28 days （chronic stage） after contusive spinal cord injury. Results showed that grafted neural progenitor cells migrated and aggregated around the blood vessels of the injured region, and infiltrated the spinal cord parenchyma along the tissue spaces in the acute stage transplantation group. However, this was not observed in subacute and chronic stage transplantation groups. 04- and glial fibrillary acidic protein-positive cells, representing oligodendrocytes and astrocytes respectively, were detected in the core of the grafted cluster attached to the cauda equina pia surface in the chronic stage transplantation group 8 weeks after transplantation. Both acute and subacute stage transplantation groups were negative for 04 and glial fibrillary acidic protein cells. Basso, Beattie and Bresnahan scale score comparisons indicated that rat hind limb locomotor activity showed better recovery after acute stage transplantation than after subacute and chronic transplantation. Our experimental findings suggest that the subarachnoid route could be useful for transplantation of neural progenitor cells at the acute stage of spinal cord injury. Although grafted cells survived only for a short time and did not differentiate into astrocytes or neurons, they were able to reach the parenchyma of the injured spinal cord and improve neurological function in rats. Transplantation efficacy was enhanced at the acute stage in comparison with subacute and chronic stages.
