Chondroitinase ABC combined with Schwann cell transplantation enhances restoration of neural connection and functional recovery following acute and chronic spinal cord injury

Schwann cell transplantation is considered one of the most promising cell-based therapy to repair injured spinal cord due to its unique growth-promoting and myelin-forming properties.A the Food and Drug Administration-approved Phase I clinical trial has been conducted to evaluate the safety of transplanted human autologous Schwann cells to treat patients with spinal cord injury.A major challenge for Schwann cell transplantation is that grafted Schwann cells are confined within the lesion cavity,and they do not migrate into the host environment due to the inhibitory barrier formed by injury-induced glial scar,thus limiting axonal reentry into the host spinal cord.Here we introduce a combinatorial strategy by suppressing the inhibitory extracellular environment with injection of lentivirus-mediated transfection of chondroitinase ABC gene at the rostral and caudal borders of the lesion site and simultaneously leveraging the repair capacity of transplanted Schwann cells in adult rats following a mid-thoracic contusive spinal cord injury.We report that when the glial scar was degraded by chondroitinase ABC at the rostral and caudal lesion borders,Schwann cells migrated for considerable distances in both rostral and caudal directions.Such Schwann cell migration led to enhanced axonal regrowth,including the serotonergic and dopaminergic axons originating from supraspinal regions,and promoted recovery of locomotor and urinary bladder functions.Importantly,the Schwann cell survival and axonal regrowth persisted up to 6 months after the injury,even when treatment was delayed for 3 months to mimic chronic spinal cord injury.These findings collectively show promising evidence for a combinatorial strategy with chondroitinase ABC and Schwann cells in promoting remodeling and recovery of function following spinal cord injury.

Pharmacological targeting cGAS/STING/NF-κB axis by tryptanthrin induces microglia polarization toward M2 phenotype and promotes functional recovery in a mouse model of spinal cord injury

The M1/M2 phenotypic shift of microglia after spinal cord injury plays an important role in the regulation of neuroinflammation during the secondary injury phase of spinal cord injury.Regulation of shifting microglia polarization from M1(neurotoxic and proinflammatory type)to M2(neuroprotective and anti-inflammatory type)after spinal cord injury appears to be crucial.Tryptanthrin possesses an anti-inflammatory biological function.However,its roles and the underlying molecular mechanisms in spinal cord injury remain unknown.In this study,we found that tryptanthrin inhibited microglia-derived inflammation by promoting polarization to the M2 phenotype in vitro.Tryptanthrin promoted M2 polarization through inactivating the cGAS/STING/NF-κB pathway.Additionally,we found that targeting the cGAS/STING/NF-κB pathway with tryptanthrin shifted microglia from the M1 to M2 phenotype after spinal cord injury,inhibited neuronal loss,and promoted tissue repair and functional recovery in a mouse model of spinal cord injury.Finally,using a conditional co-culture system,we found that microglia treated with tryptanthrin suppressed endoplasmic reticulum stress-related neuronal apoptosis.Taken together,these results suggest that by targeting the cGAS/STING/NF-κB axis,tryptanthrin attenuates microglia-derived neuroinflammation and promotes functional recovery after spinal cord injury through shifting microglia polarization to the M2 phenotype.

Optimizing the key parameter to accelerate the recovery of AMOC under a rapid increase of greenhouse gas forcing

Atlantic Meridional Overturning Circulation(AMOC)plays a central role in long-term climate variations through its heat and freshwater transports,which can collapse under a rapid increase of greenhouse gas forcing in climate models.Previous studies have suggested that the deviation of model parameters is one of the major factors in inducing inaccurate AMOC simulations.In this work,with a low-resolution earth system model,the authors try to explore whether a reasonable adjustment of the key model parameter can help to re-establish the AMOC after its collapse.Through a new optimization strategy,the extra freshwater flux(FWF)parameter is determined to be the dominant one affecting the AMOC’s variability.The traditional ensemble optimal interpolation(EnOI)data assimilation and new machine learning methods are adopted to optimize the FWF parameter in an abrupt 4×CO_(2) forcing experiment to improve the adaptability of model parameters and accelerate the recovery of AMOC.The results show that,under an abrupt 4×CO_(2) forcing in millennial simulations,the AMOC will first collapse and then re-establish by the default FWF parameter slowly.However,during the parameter adjustment process,the saltier and colder sea water over the North Atlantic region are the dominant factors in usefully improving the adaptability of the FWF parameter and accelerating the recovery of AMOC,according to their physical relationship with FWF on the interdecadal timescale.

Activation of adult endogenous neurogenesis by a hyaluronic acid collagen gel containing basic fibroblast growth factor promotes remodeling and functional recovery of the injured cerebral cortex

The presence of endogenous neural stem/progenitor cells in the adult mammalian brain suggests that the central nervous system can be repaired and regenerated after injury.However,whether it is possible to stimulate neurogenesis and reconstruct cortical layers II to VI in non-neurogenic regions,such as the cortex,remains unknown.In this study,we implanted a hyaluronic acid collagen gel loaded with basic fibroblast growth factor into the motor cortex immediately following traumatic injury.Our findings reveal that this gel effectively stimulated the proliferation and migration of endogenous neural stem/progenitor cells,as well as their differentiation into mature and functionally integrated neurons.Importantly,these new neurons reconstructed the architecture of cortical layers II to VI,integrated into the existing neural circuitry,and ultimately led to improved brain function.These findings offer novel insight into potential clinical treatments for traumatic cerebral cortex injuries.

Clinical significance of perioperative probiotic intervention on recovery following intestinal surgery

Restoring the balance of gut microbiota has emerged as a critical strategy in treating intestinal disorders,with probiotics playing a pivotal role in maintaining bacterial equilibrium.Surgical preparations,trauma,and digestive tract reconstruction associated with intestinal surgeries often disrupt the intestinal flora,prompting interest in the potential role of probiotics in postoperative recovery.Lan et al conducted a prospective randomized study on 60 patients with acute appendicitis,revealing that postoperative administration of Bacillus licheniformis capsules facilitated early resolution of inflammation and restoration of gastrointestinal motility,offering a novel therapeutic avenue for accelerated postoperative recovery.This editorial delves into the effects of perioperative probiotic supplementation on physical and intestinal recovery following surgery.Within the framework of enhanced recovery after surgery,the exploration of new probiotic supplementation strategies to mitigate surgical complications and reshape gut microbiota is particularly intriguing.

The Dantzig Selector:Sparse Signals Recovery via l_(1-q)Minimization Model

We propose the Dantzig selector based on the l_(1-q)(1<q≤2)minimization model for the sparse signal recovery.First,we discuss some properties of l_(1-q)minimization model and give some useful inequalities.Then,we give a sufficient condition based on the restricted isometry property for the stable recovery of signals.The l_(1-2)minimization model of Yin-Lou-He is extended to the l_(1-q)minimization model.

Review on strategies of space-based optical space situational awareness

Space-based optical(SBO)space surveillance has attracted widespread interest in the last two decades due to its considerable value in space situation awareness(SSA).SBO observation strategy,which is related to the performance of space surveillance,is the top-level design in SSA missions reviewed.The recognized real programs about SBO SAA proposed by the institutions in the U.S.,Canada,Europe,etc.,are summarized firstly,from which an insight of the development trend of SBO SAA can be obtained.According to the aim of the SBO SSA,the missions can be divided into general surveillance and space object tracking.Thus,there are two major categories for SBO SSA strategies.Existing general surveillance strategies for observing low earth orbit(LEO)objects and beyond-LEO objects are summarized and compared in terms of coverage rate,revisit time,visibility period,and image processing.Then,the SBO space object tracking strategies,which has experienced from tracking an object with a single satellite to tracking an object with multiple satellites cooperatively,are also summarized.Finally,this paper looks into the development trend in the future and points out several problems that challenges the SBO SSA.

Comparison of Precipitation Observations from a Prototype Space-based Cloud Radar and Ground-based Radars

A prototype space-based cloud radar has been a precipitation system over Tianjin, China in July developed and was installed on an airplane to observe 2010. Ground-based S-band and Ka-band radars were used to examine the observational capability of the prototype. A cross-comparison algorithm between different wavelengths, spatial resolutions and platform radars is presented. The reflectivity biases, correlation coefficients and standard deviations between the radars are analyzed. The equivalent reflectivity bias between the S- and Ka-band radars were simulated with a given raindrop size distribution. The results indicated that reflectivity bias between the S- and Ka-band radars due to scattering properties was less than 5 dB, and for weak precipitation the bias was negligible. The prototype space-based cloud radar was able to measure a reasonable vertical profile of reflectivity, but the reflectivity below an altitude of 1.5 km above ground level was obscured by ground clutter. The measured refiectivity by the prototype space-based cloud radar was approximately 10.9 dB stronger than that by the S-band Doppler radar （SA radar）, and 13.7 dB stronger than that by the ground-based cloud radar. The reflectivity measured by the SA radar was 0.4 dB stronger than that by the ground-based cloud radar. This study could provide a method for the quantitative examination of the observation ability for space-based radars.

Timeliness evaluation of task-oriented networked space-based information system

Task-oriented networked information system is an integrated information system which builds on multi-satellite networking to accomplish one or more tasks. In the background of emer- gency relief for applications, system working flow and response process are analyzed, and a timeliness effectiveness evaluation index system is constructed at multi-task level. The effectiveness is a measurement of promptness of information return. In evalua- tion process, system performance and tasks are associated, then an evaluation model based on efficacy function is established, and different evaluation criteria are selected for different tasks. A distributed simulation system is constructed, and the execution of task is decomposed. The simulation platform provides a comprehensive data source for evaluation. The results are easy to compare with each other, which reflects system time efficiency in different satellites networks and provides actual systems with basis and reference for design and application.

Development of long-wavelength infrared detector and its space-based application requirements

Infrared detection technology has greatly expanded the ability of mankind to study the earth and the universe. In recent years, the demand for long-wavelength infrared detectors is increasing for their advantages in exploring the earth and the universe. A variety of long-wavelength infrared detectors have been made based on thermal resistive effect, photoelectric effect, etc., in the past few decades. Remarkable achievements have been made in infrared materials, device fabrication,readout circuit, and device package. However, high performance long-wavelength infrared detectors, especially those for large format long-wavelength infrared detector focus plane array, are still unsatisfactory. Low noise, high detectivity, and large format long-wavelength infrared detector is necessary to satisfy space-based application requirements.

DDoS Attack Detection Method for Space-Based Network Based on SDN Architecture

With the development of satellite communications,the number of satellite nodes is constantly increasing,which undoubtedly increases the difficulty of maintaining network security.Combining software defined network(SDN) with traditional space-based networks provides a new class of ideas for solving this problem.However,because of the highly centralized network management of the SDN controller,once the SDN controller is destroyed by network attacks,the network it manages will be paralyzed due to loss of control.One of the main security threats to SDN controllers is Distributed Denial of Service(DDoS) attacks,so how to detect DDoS attacks scientifically has become a hot topic among SDN security management.This paper proposes a DDoS attack detection method for space-based networks based on SDN architecture.This attack detection method combines the optimized Long Short-Term Memory(LSTM) deep learning model and Support Vector Machine(SVM),which can not only make classification judgments on the time series,but also achieve the purpose of detecting and judging through the flow characteristics of a period of time.In addition,it can reduce the detection time as well as the system burden.

Understory Recovery in Coast Redwood Communities: A Case Study Comparing a Naturally Recovering and an Actively Managed Forest

Restoration of late seral features in second growth Sequoia sempervirens (coast redwood) forests is increasingly important, as so little of the original old-growth remains. Natural recovery is an effective method restoring many late seral features, and does not require the additional disturbance of active management. In order to better understand management impacts on redwood understory abundance and composition, data were collected in naturally recovering stands and in stands that were actively managed with the explicit intent of promoting old-growth characteristics. Ten 10 m diameter plots with three 2 m diameter nested sub-plots were randomly sampled in two sites within each management type. Results indicate that tree canopy cover, native species cover and richness, richness of coast redwood associated species, and the cover of Trillium ovatum (western wake robin) were significantly higher in naturally recovering versus actively managed stands. In addition, several coast redwood associated understory species were exclusively recorded in the naturally recovering stands including: Asuram caudatum (wild ginger), Prosartes hookeri (hooker’s fairybells), Maianthemum racemosum (false solomon seal), Scoliopus bigelovii (fetid adder’s tongue), Viola sempervirens (redwood violet);while only one such species was recorded exclusively in the actively managed stands: Trientalis latifolia (pacific star flower). Natural recovery appeared to support understory recovery more effectively than active forest management in this case.

Effective Methods and Performance Analysis on Data Transmission Security with Blind Source Separation in Space-Based AIS

In space-based Automatic Identification Systems(AIS), due to high satellite orbits, several Ad Hoc cells within the observation range of the satellite are vulnerable to interference by an external signal.To increase efficiency in target detection and improve system security, a blind source separation method is adopted for processing the conflicting signals received by satellites. Compared to traditional methods, we formulate the separation problem as a clustering problem. Since our algorithm is affected by the sparseness of source signals, to get satisfactory results, our algorithm assumes that the distance between two arbitrary mixed-signal vectors is less than the doubled sum of variances of distribution of the corresponding mixtures. Signal sparsity is overcome by computing the Short-Time Fourier Transform, and the mixed source signals are separated using the improved PSO clustering. We evaluated the performance and the robustness of the proposed network architecture by several simulations. The experimental results demonstrate the effectiveness of the proposed method in not only improving satellite signal receiving ability but also in enhancing space-based AIS security.

Some Fundamental Problems of Geodynamics and Application of Earth Observations by Space-Based Measurements

Although great-progress has been made in the earth sciences,some fundamental problems of geodynamics remain unsolved.They are concerned with the whole earth as well as regional tectonics,such as the west Pacific and Qinghai-Xizhang plateau.The new generation of earth observation by space-based measurement will contribute to solving these problems of geodynamics.In this regard,some specific plans about application of these techniques are suggested in this paper.

Activation of cerebral Ras-related C3 botulinum toxin substrate(Rac) 1 promotes post-ischemic stroke functional recovery in aged mice

Brain functional impairment after stroke is common;however,the molecular mechanisms of post-stroke recovery remain unclear.It is well-recognized that age is the most important independent predictor of poor outcomes after stroke as older patients show poorer functional outcomes following stroke.Mounting evidence suggests that axonal regeneration and angiogenesis,the major forms of brain plasticity responsible for post-stroke recovery,diminished with advanced age.Previous studies suggest that Ras-related C3 botulinum toxin substrate(Rac)1 enhances stroke recovery as activation of Rac1 improved behavior recovery in a young mice stroke model.Here,we investigated the role of Rac1 signaling in long-term functional recovery and brain plasticity in an aged(male,18 to 22 months old C57BL/6J)brain after ischemic stroke.We found that as mice aged,Rac1 expression declined in the brain.Delayed overexpression of Rac1,using lentivirus encoding Rac1 injected day 1 after ischemic stroke,promoted cognitive(assessed using novel object recognition test)and sensorimotor(assessed using adhesive removal tests)recovery on days 14–28.This was accompanied by the increase of neurite and proliferative endothelial cells in the periinfarct zone assessed by immunostaining.In a reverse approach,pharmacological inhibition of Rac1 by intraperitoneal injection of Rac1 inhibitor NSC23766 for 14 successive days after ischemic stroke worsened the outcome with the reduction of neurite and proliferative endothelial cells.Furthermore,Rac1 inhibition reduced the activation of p21-activated kinase 1,the protein level of brain-derived neurotrophic factor,and increased the protein level of glial fibrillary acidic protein in the ischemic brain on day 28 after stroke.Our work provided insight into the mechanisms behind the diminished plasticity after cerebral ischemia in aged brains and identified Rac1 as a potential therapeutic target for improving functional recovery in the older adults after stroke.

Relic Gravity Waves Investigation by Advanced Space-Based Gravitational Waves Detector

This paper focuses on the relic gravity waves produced during the transition from a radiation-dominated inflationary phase to a dust-dominated Friedman-Robertson-Walker-type expansion. We discuss how to investigate the spectral energy density by the latest space-based CWs detectors at f =0.1 Hz （i.e. DECICO）. In the case of power-law and exponential inflation, we apply the cross-correlation method to the latest detector and get the time dependence of the very early Hubble pararneter.

Is recovery enhancement after gastric cancer surgery really a safe approach for elderly patients?

BACKGROUND This study aimed to evaluate the safety of enhanced recovery after surgery(ERAS)in elderly patients with gastric cancer(GC).AIM To evaluate the safety of ERAS in elderly patients with GC.METHODS The PubMed,EMBASE,and Cochrane Library databases were used to search for eligible studies from inception to April 1,2023.The mean difference(MD),odds ratio(OR)and 95%confidence interval(95%CI)were pooled for analysis.The quality of the included studies was evaluated using the Newcastle-Ottawa Scale scores.We used Stata(V.16.0)software for data analysis.RESULTS This study consists of six studies involving 878 elderly patients.By analyzing the clinical outcomes,we found that the ERAS group had shorter postoperative hospital stays(MD=-0.51,I2=0.00%,95%CI=-0.72 to-0.30,P=0.00);earlier times to first flatus(defecation;MD=-0.30,I²=0.00%,95%CI=-0.55 to-0.06,P=0.02);less intestinal obstruction(OR=3.24,I2=0.00%,95%CI=1.07 to 9.78,P=0.04);less nausea and vomiting(OR=4.07,I2=0.00%,95%CI=1.29 to 12.84,P=0.02);and less gastric retention(OR=5.69,I2=2.46%,95%CI=2.00 to 16.20,P=0.00).Our results showed that the conventional group had a greater mortality rate than the ERAS group(OR=0.24,I2=0.00%,95%CI=0.07 to 0.84,P=0.03).However,there was no statistically significant difference in major complications between the ERAS group and the conventional group(OR=0.67,I2=0.00%,95%CI=0.38 to 1.18,P=0.16).CONCLUSION Compared to those with conventional recovery,elderly GC patients who received the ERAS protocol after surgery had a lower risk of mortality.

Multidisciplinary approach toward enhanced recovery after surgery for total knee arthroplasty improves outcomes

Knee osteoarthritis is a degenerative disorder of the knee,which leads to joint pain,stiffness,and inactivity and significantly affects the quality of life.With an increased prevalence of obesity and greater life expectancies,total knee arthroplasty(TKA)is now one of the major arthroplasty surgeries performed for knee osteoarthritis.When enhanced recovery after surgery(ERAS)was introduced in TKA,clinical outcomes were enhanced and the economic burden on the healthcare system was reduced.ERAS is an evidence-based scientific protocol aimed at ameliorating the surgical stress response.ERAS aims to enhance the recovery phase,which encompasses multidisciplinary strategies at every step of perioperative care,including the rehabilitation phase.Implementation of ERAS in TKA aids in reducing the length of hospital stay,improving pain management,reducing perioperative complications,and enhancing patient satisfaction.Multidisciplinary collaboration,integrating the expertise of anesthesiologists,orthopedic surgeons,nursing personnel,and other healthcare professionals,is the cornerstone of ERAS in patients undergoing TKA.

Transplantation of fibrin-thrombin encapsulated human induced neural stem cells promotes functional recovery of spinal cord injury rats through modulation of the microenvironment

Recent studies have mostly focused on engraftment of cells at the lesioned spinal cord,with the expectation that differentiated neurons facilitate recovery.Only a few studies have attempted to use transplanted cells and/or biomaterials as major modulators of the spinal cord injury microenvironment.Here,we aimed to investigate the role of microenvironment modulation by cell graft on functional recovery after spinal cord injury.Induced neural stem cells reprogrammed from human peripheral blood mononuclear cells,and/or thrombin plus fibrinogen,were transplanted into the lesion site of an immunosuppressed rat spinal cord injury model.Basso,Beattie and Bresnahan score,electrophysiological function,and immunofluorescence/histological analyses showed that transplantation facilitates motor and electrophysiological function,reduces lesion volume,and promotes axonal neurofilament expression at the lesion core.Examination of the graft and niche components revealed that although the graft only survived for a relatively short period(up to 15 days),it still had a crucial impact on the microenvironment.Altogether,induced neural stem cells and human fibrin reduced the number of infiltrated immune cells,biased microglia towards a regenerative M2 phenotype,and changed the cytokine expression profile at the lesion site.Graft-induced changes of the microenvironment during the acute and subacute stages might have disrupted the inflammatory cascade chain reactions,which may have exerted a long-term impact on the functional recovery of spinal cord injury rats.

Synergistic anionic/zwitterionic mixed surfactant system with high emulsification efficiency for enhanced oil recovery in low permeability reservoirs

Emulsification is one of the important mechanisms of surfactant flooding. To improve oil recovery for low permeability reservoirs, a highly efficient emulsification oil flooding system consisting of anionic surfactant sodium alkyl glucosyl hydroxypropyl sulfonate(APGSHS) and zwitterionic surfactant octadecyl betaine(BS-18) is proposed. The performance of APGSHS/BS-18 mixed surfactant system was evaluated in terms of interfacial tension, emulsification capability, emulsion size and distribution, wettability alteration, temperature-resistance and salt-resistance. The emulsification speed was used to evaluate the emulsification ability of surfactant systems, and the results show that mixed surfactant systems can completely emulsify the crude oil into emulsions droplets even under low energy conditions. Meanwhile,the system exhibits good temperature and salt resistance. Finally, the best oil recovery of 25.45% is achieved for low permeability core by the mixed surfactant system with a total concentration of 0.3 wt%while the molar ratio of APGSHS:BS-18 is 4:6. The current study indicates that the anionic/zwitterionic mixed surfactant system can improve the oil flooding efficiency and is potential candidate for application in low permeability reservoirs.