Investigation of lacrimal sac space-occupying lesions using color doppler ultrasound, computed tomography, and computed tomography dacryocystography

AIM:To observe the imaging features of color Doppler ultrasound(CDU)and computed tomography(CT)or computed tomography dacryocystography(CT-DCG)in different types of lacrimal sac space-occupying lesions(SOLs).METHODS:This retrospective case series study included 21 patients with lacrimal sac SOLs who underwent lacrimal sac surgery between January 2018 and March 2022.The imaging features of CDU and CT or CT-DCG in these patients were extracted from the examination cloud system.The images were observed and analyzed.RESULTS:The detection rate of lacrimal SOLs between CDU(21/21,100%)and CT or CT-DCG(20/21,95.2%)had no statistically significant difference(P=1.0).CDU could detect the blood flow signals in all SOLs except mucocele and mucopeptide concretion.Among them,polyps had characteristic imaging changes on CDU and CT-DCG.The mucoceles and mucopeptide concretions had characteristic imaging changes on CDU,which could provide more information for differential diagnosis.CONCLUSION:The morphology and internal blood flow signals of lacrimal sac SOLs can be observed using CDU.CT or CT-DCG has advantages in observing structural damage around the lacrimal sac mass.Therefore,CDU may be used as a routine examination to exclude lacrimal sac SOLs before dacryocystorhinostomy in the absence of preoperative CT or CT-DCG.

Pneumocephalus Following Combined Spinal-Epidural Anesthesia: A Case Report Analysis

Background: Combined spinal-epidural anesthesia (CSEA) is widely used in clinical anesthesia due to its rapid onset, reliable anesthetic effect, and strong controllability. Although advancements in technique have reduced the frequency and severity of common complications, reports of rare and serious complications such as pneumocephalus, remain scarce. Case Report: This article presents a case of pneumocephalus following CSEA in a middle-aged female patient undergoing surgery for an intrauterine space-occupying lesion. The patient experienced severe headache postoperatively, and imaging confirmed the presence of intracranial air. After receiving active symptomatic treatment, the patient recovered and was discharged. Conclusion: This case underscores the importance of adhering to standard anesthesia protocols and increasing awareness of rare CSEA complications, particularly the risk of pneumocephalus. Early recognition and timely management are crucial. There is a need to further enhance training and research in anesthetic procedures to improve clinical anesthesia quality and ensure patient safety.

Assessment of optic nerve and optic tract alterations in patients with orbital space-occupying lesions using probabilistic diffusion tractography

AIM: To investigate the diffusion changes in both the optic nerve and optic tract in orbital space-occupying lesion patients with decreased visual acuity, and its clinical significance using probabilistic diffusion tractography(PDT). METHODS: Twenty patients with orbital space-occupying lesions and 25 age-and gender-matched healthy persons were included. All patients and controls underwent routine orbital magnetic resonance imaging and diffusion tensor imaging(DTI), using a 3.0 T magnetic resonance scanner(Trio Tim Siemens). After the image data were preprocessed, each DTI parameters of the optic nerve and optic tract was obtained by PDT, including fractional anisotropy(FA), mean diffusivity(MD), axial diffusivity(AD) and radial diffusivity(RD). The asymmetry index(AI) of each parameter was calculated. Compared the parameters of the affected side optic nerve and ipsilateral optic tract with the contralateral side by paired sample t-test;compared AI of parameters of optic nerve and optic tract between the patient group and the control group by independent sample t-test. Patients were divided into threesubgroups according to the low vision grade standard of WHO, compared the FA and AI of FA between the three subgroups by single factor variance analysis. RESULTS: The affected side optic nerve presented significantly decreased FA, increased MD, AD, and RD values compared to the unaffected side(P<0.05). The AI of FA, MD, AD, and RD of optic nerve in the patients was significantly higher than that of the controls(P<0.05). The comparison results of the optic tract showed that there was no significant difference between the patient group and control group in terms of the bilateral optic tracts in patients(P>0.05). The AIs of the FA value of the optic nerve in the eyesight <0.1 subgroup was significantly higher than that in the other groups(P<0.05). CONCLUSION: FA, MD, AD, and RD of the affected side optic nerve of the orbital space-occupying lesions have significantly changed, the FA value is the most sensitive. The PDT could be a useful tool to provide valid quantitative markers of optic nerve injuries and evaluate the severity of orbital diseases, which other examinations cannot be acquired.

Application of endoscopic submucosal dissection in duodenal space-occupying lesions

BACKGROUNDEndoscopic submucosal dissection (ESD) has been advocated by digestiveendoscopists because of its comparable therapeutic effect to surgery, reducedtrauma, faster recovery, and fewer complications. However, ESD for lesions of theduodenum is more challenging than those occurring at other levels of thegastrointestinal tract due to the thin intestinal wall of the duodenum, narrowintestinal space, rich peripheral blood flow, proximity to vital organs, and highrisks of critical adverse events including intraoperative and delayed bleeding andperforation. Because of the low prevalence of the disease and the high risks ofsevere adverse events, successful ESD for lesions of the duodenum has rarelybeen reported in recent years.AIM To investigate the efficacy and safety of ESD in the treatment of duodenal spaceoccupyinglesions.METHODS Clinical data of 24 cases of duodenal lesions treated by ESD at the DigestiveEndoscopy Center of the Affiliated Hospital of Qingdao University from January2016 to December 2019 were retrospectively analyzed.RESULTS All of the 24 cases from 23 patients underwent ESD treatment for duodenal spaceoccupyinglesions under general anesthesia, including 15 male and 8 femalepatients, with a mean age of 58.5 (32.0-74.0) years. There were 12 lesions (50%) inthe duodenal bulb, 9 (37.5%) in the descending part, and 3 (12.5%) in the ball descending junction. The mean diameter of the lesion was 12.75 (range, 11-22)mm. Thirteen lesions originated from the mucosa, of which 4 were low-gradeintraepithelial neoplasia, 3 were hyperplastic polyps, 2 were chronic mucositis, 2were adenomatous hyperplasia, 1 was high-grade intraepithelial neoplasia, and 1was tubular adenoma. Eleven lesions were in the submucosa, including 5neuroendocrine neoplasms, 2 cases of ectopic pancreas, 1 stromal tumor, 1leiomyoma, 1 submucosal duodenal adenoma, and 1 case of submucosal lymphfollicular hyperplasia. The intraoperative perforation rate was 20.8% (5/24),including 4 submucosal protuberant lesions and 1 depressed lesion. The meanlength of hospital stay was 5.7 (range, 3-10) d, and the average follow-up time was25.8 (range, 3.0–50.0) mo. No residual disease or recurrence was found in allpatients, and no complications, such as infection and stenosis, were found duringthe follow-up period.CONCLUSION ESD is safe and effective in the treatment of duodenal lesions;however, theendoscopists should pay more attention to the preoperative preparation,intraoperative skills, and postoperative treatment.

Recurrent orbital space-occupying lesions:a clinicopathologic study of 253 cases

Objective：To analyze the clinical features,histopathologic classification and frequencies of various types of recurrent orbital space-occupying lesions.Methods：A retrospective study was carried out in 253 consecutive patients with recurrent orbital spaceoccupying lesions treated by surgical excision in the Institute of Orbital Diseases,the General Hospital of the Armed Police Force from January 2009 to December 2010.Results：The patients included 123 males and 130 females aged 2 to 78 years（mean,36.2 years）,and the last recurrence interval after operation ranged from 1 month to 40 years（median,4.75 years）.Of all the cases,159（62.8%）,65（25.7%）,20（7.9%）,8（3.2%） and 1（0.4%） had previously experienced once,twice,three,four and six times of surgeries,respectively.Among them,29（11.5%） cases had recurred 3 times or over,and 37（14.6%） cases got recurrence in 10 or more years postoperatively.Most of the patients with local recurrence presented with various clinical manifestations,while 31（12.3%） cases were symptom-free.Two hundred and thirty-one（91.3%） cases underwent surgical removal of the recurrent orbital lesions,and another 22（8.7%） cases had to receive the exenteration of orbit.Categories of these recurrent orbital lesions after operation were as follows：lacrimal gland tumors,65（25.7%） cases;vasogenic diseases,54（21.3%） cases;neurogenic tumors,42（16.6%） cases;secondary tumors,24（9.5%） cases;orbital inflammation,21（8.3%） cases;myogenic tumors,14（5.5%） cases;fibrous and adipose tumors,12（4.7%） cases;lympho-hematopoietic tumors,7（2.8%） cases;bone or cartilage tumors,7（2.8%） cases;orbital cysts,6（2.4%） cases;and indefinitely differentiated tumor,1（0.4%） case.The 10 top histopathologic diagnoses were lacrimal gland pleomorphic adenoma,hemangiolymphangioma,lacrimal gland adenoid cystic carcinoma,meningioma,inflammatory pseudotumor,neurofibroma,sebaceous gland carcinoma,vascular malformation,rhabdomyosarcoma and hemangioma.Conclusions：The variety of recurrent orbital lesions after operation includes mainly of tumors except for vascular malformation and orbital inflammatory lesions.The lacrimal gland epithelial tumor is most prone to relapse after resection,and early and longer-term postoperative follow-up is needed.

Association between the fMRI manifestations of activated brain areas and muscle strength in patients with space-occupying lesions in motor cortex

BACKGROUND ： Functional magnetic resonance imaging （fMRI） studies have disclosed the changes of the motor function in the motor cortex of ipsilateral and contralateral hemispheres of tumor, which have special significance for making the surgical planning and most greatly minimizing the postoperative functional damages. OBJECTIVE： To analyze the association between the manifestation characteristics of hand functional area and motor dysfunction using fMRI in patients with space-occupying lesions of tumor in motor cortex. DESIGN ： A case-controlled observation SETTING： Department of Radiology, Second Affiliated Hospital, Shantou University Medical College .PARTICIPANTS： Twenty-three patients （13 males and 10 females） with space-occupying lesions of central sulcus area, aged 21-53 years with a mean age of （47±1） years were selected from the Second Affiliated Hospital of Shantou University Medical College. All the patients were diagnosed by MR scanning as space-occupying lesions of motor area, and pathologically confirmed that the lesions involved central sulcus and central Iobule; Lesions occurred in left and right hemispheres in 13 and 10 cases respectively. The tumor types were astrocytoma （n =8）, metastatic tumours （n =7）, meningiomas （n =5） and oligodendroglioma （n =3）. The muscle strength was normal in 11 cases （grade 5） and obviously decreased in 12 cases （grade 2-3 in 3 cases and grade 4 in 9 cases）; muscle strengths of both upper and lower limbs were decreased in 7 cases, and only that of upper limbs was decreased in 5 cases. Informed consents were obtained from all the subjects. Meanwhile, 9 healthy physical examinees （5 males and 4 females） of 20-56 years old with an average of （34±1） years were taken as controls. All the patients and healthy subjects were right-handed. METHODS： All the enrolled subjects were examined with MR scanning and functional imaging. Twenty cases whose clinical symptoms were mild in the patient group and 9 healthy volunteers adopted simple active finger-tapping movements, and for the 3 cases whose clinical symptoms were severe in the patient group, the simple passive finger-tapping movements were used. The manifestations in the activated brain areas were analyzed in the patients with brain tumor of different muscle strength and the controls. The motor deficit and activation of contralateral primary motor cortex （M1） in simple finger-tapping movements were observed in the patient group. MAIN OUTCOME MEASURES： （1） Brain areas activated by finger-tapping movements in each group： （2） Activated volumes in hemisphere by finger-tapping movements between groups. RESULTS： The contralateral M1 area could not be activated in 1 case in the patient group,, all the other 22 patients and 9 healthy subjects were involved in the analysis of results. （1） In the control group, unilateral finger tapping movement activated the contralateral primary motor cortex （M1）, bilateral SMA and bilateral PMC. The activation volume was the largest in contralateral primary motor cortex （M1）, smaller in the SMA, and the smallest in PMC. The finger tapping movement in healthy subjects could activate contralateral primary motor cortex （M1）, bilateral SMA and bilateral PMC, which had no obvious differences from the manifestations of brain functional area activated by active finger tapping. There was no significant difference in the volume of activated functional areas between right and left hands. In the patient group, the central sulcos around the tumor in the activated M1 area displaced towards dorsal or ventral side, also extended. The distance of displacement in the functional area was determined as compared with the contralateral central sulcus, and the results suggested the M1 displacement, including that there were 10 cases with the M1 displacement larger than 10 mm in the patients with motor deficit, which were obviously more than in those without motor deficit （n =1, P 〈 0.01）, and the activated volume in contralateral M1 area was obvious smaller in the patients with motor deficit than in those without motor deficit （P 〈 0.01）. （2） The M1 activation and changes were observed in contralateral hemisphere in the patient group, and the activated volume was obviously larger than that in the control group （P 〈 0.01）. The activated volumes of M1 and PMC in ipsilateral hemisphere were obviously larger than those in the control group （P 〈 0.05）, but that of SMA had no obvious difference between the two groups （P 〉 0.05）. CONCLUSION： fMRI can be used to observe the activation of the brain motor functional areas of patient with space-occupying lesions in motor area, and evaluate the state of their motor function. The larger the distance of displacement of M1 compressed by tumor, the more obviously the muscle strength decreases in the patients.

脑实质内小占位病灶的手术治疗

报告手术治疗脑内小占位病灶４５例，其中经颅骨钻孔ＣＴ立体定向下摘除病灶１９例，而３例是以显微手术完成的。在ＣＴ立体定向下用显微手术摘除脑实质内小占位病灶具有定位准确，更易保护脑组织及血管，使术后患者脑组织功能保持或优于术前水平。

椎管内占位性病变的手术治疗

本文报告６１例椎管内占位性病变的手术治疗方法，其部位有：颈椎行“单开门术”暴露椎管，胸椎用电磨行全椎板切除，腰椎则行保留棘上韧带、棘间韧带和棘突的次全椎板切除，并对椎管内外哑铃型肿瘤的手术方法及其它注意事项进行了讨论。

结直肠锯齿状息肉的临床病理及7种抗体表达

目的探讨结直肠锯齿状息肉的临床病理特征及MLH1、MGMT、RUNX3、CDX2、β-catenin、p16和Ki-67的表达。方法收集增生性息肉(HP)52例,其中微小泡状型增生性息肉(MVHP)38例,杯状细胞型增生性息肉(GCHP)12例,黏液缺乏型增生性息肉(MPHP)2例,观察其临床病理特征,同时进行免疫组化染色;并以广基锯齿状息肉(SSA)41例、传统锯齿状腺瘤(TSA)23例、正常结直肠黏膜组织24例及结直肠腺癌(CRC)24例作为对照。结果 HP多发生于左半结肠,可分为MVHP、GCHP和MPHP 3种亚型。主要表现为大部分腺腔有锯齿状结构,隐窝拉长,多呈管状,隐窝上部腔缘呈锯齿状,增生部分位于基底及下1/3,隐窝下1/3狭窄,间隔被覆未分化细胞及神经内分泌细胞。RUNX3、β-catenin、p16和Ki-67的表达阳性率,HP与其他各组比较差异显著(P<0.05)。结论 HP为最常见锯齿状病变,大部分腺腔呈锯齿状改变,伴/不伴杯状细胞。RUNX3、β-catenin与p16在HP的发生、发展过程中可能起重要作用。MPHP增殖活性较高可能有一定的恶性潜能。

课程教学目标设计中的概念表述问题与教师的备课实践选择

20世纪以来,随着教育心理学的发展,我国课程教学目标设计领域出现了许多新理论,比如有以苏联教育模式来设计课程教学目标,也有按照美国的教学理论来设计,或者有些学者和教师侧重全面发展教育理论。我们认为,课程标准制定和教师课堂教学方案设计应从认知目标、情感目标、行为目标和人格目标几方面来实现学生全面发展和素质教育理念。

IABP预处理在合并左室收缩功能不全CTO病变介入治疗中的价值

【目的】探讨在合并左室收缩功能不全慢性闭塞病变(CTO)PCI过程中主动脉内球囊反搏(IABP)预处理的临床意义。【方法】回顾性分析2004年到2011年南京市第一医院心内科,因合并左室收缩功能不全CTO患者而行PCI及IABP治疗的患者63例,按照IABP置入时间分组为PCI术前IABP预处理组和PCI术后IABP组。记录两组患者的基线临床资料,比较两组患者术中靶病变成功血运重建率,无复流发生率,PCI相关心肌梗死发生率,术后一年内全因死亡率。【结果】两组的靶病变血运重建率IABP预处理组为75.2%,术后IABP组为63.2%(P>0.05);IABP预处理组无复流发生率为16.3%,术后IABP组无复流发生率为42.1%(P<0.05);PCI相关心肌梗死发生率IABP预处理组为75.6%,术后IABP组为52.6%(P<0.05);术后一年内全因死亡率分别为:IABP预处理组为18.6%,术后IABP组为31.6%(P>0.05)。【结论】在合并左室收缩功能不全CTO病变介入治疗中,IABP预处理较术后IABP能显著减少术中无复流发生率,显著减轻术后PCI相关心肌梗死发生率,对靶病变血运重建率无显著影响,对一年内全因死亡率无显著影响。

开放性立体定向锁眼手术切除颅内小病灶

目的 探讨开放性立体定向“锁眼”手术切除颅内小病灶的手术方法和优越性。方法 2 0例颅内小病灶 (直径在 0 .6～ 3cm)在CRW立体定向仪及手术计划系统指导下 ,采用小切口 ,环钻开颅 ,显微外科手术 ,表浅病灶直接沿脑沟分离完整切除 ,功能区皮层下病灶 ,在导针引导下沿非功能区皮层下斜形径路分块切除。结果 在CRW定向系统精确定位下 ,病灶全切率达 10 0 %,5例功能区病灶术后神经功能保护良好 ,无手术并发症及死亡。结论 立体定向引导的“锁眼”显微手术切除颅内小病灶 ,是一种定位精确、微创、安全、有效的手术方法。

宫颈锥切范围的临床研究

目的 探讨生育年龄女性因宫颈高级别鳞状上皮内病变行宫颈锥形切除术的适宜范围。方法 将98例因宫颈高级别鳞状上皮内病变行宫颈锥切术的有生育要求的生育年龄女性患者分成A、B组,A组51例,B组47例,两组各有2例阴道镜示不满意,转化区为3型。两组锥底宽度为碘不着色区外3~5 mm;A、B组锥切高度为10~15、16~25 mm。对两组手术时间、术中出血量、住院时间、切缘阳性率、术后晚期出血及宫颈粘连、治愈时间及术后3、6个月随访结果统计分析。结果 A、B组手术时间分别为（15±5.5）、（18±5.2）min,术中出血量分别为（20±9.5）、（28±10.5）ml（P〈0.001）,差异有统计学意义（P〈0.05）。A、B组住院时间分别为（2.0±0.64）、（2.0±0.73）d,差异无统计学意义（P〉0.05）。A组切缘阳性率3.9%（2例）,B组切缘阳性率2.1%（1例）,均为3型转化区患者,1个月后此3例再次宫颈补切后切缘转为阴性。术后2周左右,B组发生宫颈创面大出血1例,行缝扎止血;A组无大出血病例。两组无1例宫颈粘连或狭窄发生。术后4~5周随访结果显示：两组宫颈伤口均已痊愈,宫颈形状均恢复良好。术后3、6个月复诊,两组TCT均阴性,A组有2例（3.9%）HPV-DNA阳性,B组1例（2.1%）HPV-DNA阳性,差异均无统计学意义（P〉0.05）结论 患宫颈高级别鳞状上皮内病变的生育年龄女性,如宫颈转化区为1和2型,高度仅需10~15 mm的宫颈锥切术将有利于今后的妊娠结局,而3型转化区患者则需切除更深更多的组织方能保证切净病变组织。

原发性高血压患者微量清蛋白尿与冠脉病变的相关分析

目的探讨原发性高血压患者微量清蛋白尿水平与冠脉病变程度的关系。方法选取行冠脉造影的原发性高血压患者80例,根据冠脉造影结果分为非多支病变组(n=34)和多支病变组(n=46),测定并比较两组患者尿清蛋白/肌酐值(ACR)水平。结果 ACR水平在多支病变组高于非多支病变组(P<0.05)。结论原发性高血压患者中,微量清蛋白尿对冠脉病变的严重程度有一定的预测价值。

微课翻转课堂引入普通高校体育业余训练的实证研究

为适应信息化教学方式改革,优化现有教学环境,提高学生自主性和参与性,探索微课翻转课堂在普通高校业余训练教学的理论与方法。采用文献法、实验分、逻辑分析法等进行研究,得出结论:(1)翻转课堂引入普通高校体育业余训练教学中具有可行性;(2)翻转课堂引入普通高校体育业余训练中提高课堂实效性;(3)翻转课堂对学生的自觉性具有较高要求;(4)翻转课堂对教师信息技术能力提出了挑战。

大白鼠实验性胃癌的形态学及ConA、PNA受体的观察

用MNNG复制Wistar大白鼠腺胃癌。选用31只难性大白鼠,体重为110～120g,给25只大白鼠白山饮服MNNG100 mg/L为实验组,6只大白鼠给饮自来水作为对照组。实验组分别于实验后24、44、64、68周处死或自然死亡;对照组于44、64、68周各处死2只。于68周时处死解剖之12只大鼠小,有6只产生腺胃高分化腺癌并伴癌前病变。ConA、PNA受体的标志,发现对照组及无癌形成实验组大鼠腺胃ConA受体呈膜性分布,PNA受体除壁细胞、主细胞外,均呈阴性,于癌灶及不典型增生区显示膜性分布及浆性分布,表示糖基有改变。

浦东新区贫困妇女子宫颈病变状况分析

目的了解分析浦东新区贫困妇女子宫颈病变状况。方法采用液基细胞学(LCT)结合阴道镜及组织学检查对1036例浦东新区贫困妇女(贫困组)进行宫颈病变筛查,并以同期1019例非贫困妇女的妇科普查资料为对照(对照组),分析其细胞学及病理结果。结果贫困组LCT结果为未明确意义的不典型鳞状上皮细胞(ASCUS)96例、不典型腺上皮细胞(AGC)1例、轻度鳞状上皮内病变(LSIL)5例、重度鳞状上皮内病变(HSIL)2例;对照组ASCUS65、AGC4例、LSIL2例,无HSIL;两组细胞学异常率分别为10.04%(104/1036)和6.87%(70/1019),具有显著差异(P<0.01)。以45岁为界,≤45岁的妇女中贫困较非贫困LCT异常细胞检出率高,分别为10.55%(54/512)和6.88%(63/916),具有统计学差异(P<0.05),而>45岁,二者无显著差异。LCT结果为ACG、LSIL、HSIL及贫困组46例ASCUS和对照组48例ASCUS的妇女进行了阴道镜下活检。贫困组共有54例进行了阴道镜下活检,病理结果为CINⅠ、Ⅱ、Ⅲ分别有5、9、6例,1例为宫颈癌;对照组共有54例进行阴道镜下活检,病理结果为CINⅠ、Ⅱ、Ⅲ分别有3、2、1例,未发现宫颈癌;CIN发生率两组分别为37.04%和11.11%,差异显著(P<0.005)。结论贫困妇女子宫颈疾病的发生率高,在扶贫力度有限的情况下,首先应考虑育龄贫困妇女子宫颈疾病筛查。对于从未参加过妇科普查的老年妇女,也应列入筛查范围。

面部肉芽肿性感染合并颅内占位病变1例

患者女,61岁。面部浸润性红斑2年,头晕、右侧肢体乏力半个月。皮肤科情况:鼻部、眶周、面颊以鼻梁为中心的大片蝶状暗红色浸润性皮损。组织病理结合免疫组化示:感染性肉芽肿改变,主要为浆细胞、淋巴和组织细胞浸润,考虑炎症性病变,伴浆细胞浸润。头颅MRI示:左侧顶叶占位,两侧额窦、筛窦、上颌窦炎症。诊断:面部肉芽肿性感染合并颅内占位病变。手术切除占位病变后,给予药物联合治疗,1个月后皮损基本消退。

细胞因子的变化与宫颈癌、宫颈上皮增生病变临床关系的探讨

应用双抗体夹心ELISA法测定110例宫颈上皮增生病变和30例宫颈癌患者TNFα、sIL-2R、IL-6和IL-8的含量。结果:宫颈上皮增生病变各组患者TNFα、sIL-2R、IL-6和IL-8水平均明显高于对照组(均P<0.01),且随宫颈上皮病变程度的加重而相应地升高。宫颈癌组细胞因子变化的特点:(1)TNFα和sIL-2R水平增高显著,与宫颈上皮内瘤样病变(CIN)各病变组有明显差异(均P<0.01)。(2)IL-6和IL-8水平未见明显改变。表明细胞因子的变化与宫颈上皮增生病变的进展以及宫颈癌患者的免疫抑制状态相关。因此,细胞因子的测定对于宫颈癌的早期辅助诊断和预后的判断有重要的临床意义。

巴仙送子汤对生精障碍小鼠睾丸组织及其精子影响的实验研究

目的:研究巴仙送子汤对生精障碍模型小鼠的影响,为临床应用和进一步研究巴仙送子汤提供客观的实验和理论依据。方法:取健康成年雄性小鼠,麻醉成功后将其睾丸浸没于43℃温水浴20 min造模,随机分成三组,模型对照组(简称模型组)常规喂养14 d,不予药物干预,另外两组分别灌服巴仙送子汤(简称巴仙组)和五子衍宗汤(简称五子组)连续14 d。另设正常组,常规喂养相同时间。处死后,检测附睾精子密度、活动率,并做睾丸组织光镜病理切片观察。结果:巴仙送子汤能明显促进损伤睾丸病理恢复正常,优于五子组及模型组;巴仙送子汤能明显提高小鼠精子活动率,优于五子组(P<0.01)及正常组(P<0.05);增加小鼠精子密度优于五子组(P<0.01)。结论:巴仙送子汤有明显的促生精、恢复睾丸病理的作用,且优于五子组与模型组。