Proteus mirabilis, a motile Gram-negative bacterium, represents a common cause of complicated urinary tract infections. Autotransporters are a family of secreted proteins from Gram-negative bacteria that direct their ...Proteus mirabilis, a motile Gram-negative bacterium, represents a common cause of complicated urinary tract infections. Autotransporters are a family of secreted proteins from Gram-negative bacteria that direct their own secretion across the outer membrane (type V autotransporter secretion mechanism). Serine protease autotransporters of Enterobacteriaceae (SPATEs) include adhesins, toxins, and proteases that can contribute to the virulence. Plasmid-encoded toxin (Pet) is the predominant protein in culture supernatants of enteroaggregative E. coli prototype strain 042 and has been extensively studied. Pet toxin is encoded on the 65-MDa adherence-related plasmid of EAEC 042 strain. In this work, Pet protein was found in the supernatant obtained from Proteus mirabilis RTX339 strain isolated from a psychiatric patient suffering complicated urinary tract infections (UTIs). The nucleotide sequence of pet gene was obtained using primers designed from E. coli 042 pet gene reported. The alignment of the sequence showed 100% identity with the pet gene reported. Is important to note that Proteus mirabilis RTX339 pet gene has chromosomal location. The chromosomal location of the gene was established since no plasmids were harbored by this strain.展开更多
Climate change impacts on water resources are expected to be significant in Yemen. Efforts have been made to understand the expected changes and develop mitigation possibilities for the expected scenarios for a future...Climate change impacts on water resources are expected to be significant in Yemen. Efforts have been made to understand the expected changes and develop mitigation possibilities for the expected scenarios for a future sustainable use of resources and mitigation of expected impacts. The paper describes the development of a detailed baseline database and the assessment of climate change and variability impacts on water resources over the 2030, 2050 and 2080 time horizon on a Yemen-wide scale. Based on downscaled Global Climate Model data, a range of scenarios were established, representing potential Mean, Warm ＆ Wet and Hot ＆ Dry conditions as derived by evaluating worst case scenarios from the ensemble of the global models for the specified years. The results of the model include an estimated runoff coefficient, monthly rainfall, runoff, infiltration and evaporation representing the water balance in the different catchments. Analysis of the different evaluated scenarios shows that in the Mid, Warm and Wet scenario the hydrological components are generally higher than in the baseline scenario. For the Hot ＆ Dry scenario, runoff, infiltration and evapotranspiration are decreasing due to the decreasing precipitation and increase in temperature. The relative changes in runoff are strongest.展开更多
The spate of deadly mass shootings in the United States have caused concern both domestically and in the international community. According to a report by the China Society for Human Rights Studies, a member of the Un...The spate of deadly mass shootings in the United States have caused concern both domestically and in the international community. According to a report by the China Society for Human Rights Studies, a member of the United Nations Conference of Non-Governmental Organizations, the rampant gun violence reflects the profound crisis in the U.S. political and social systems and highlights the U.S. hypocrisy on human rights.展开更多
文摘Proteus mirabilis, a motile Gram-negative bacterium, represents a common cause of complicated urinary tract infections. Autotransporters are a family of secreted proteins from Gram-negative bacteria that direct their own secretion across the outer membrane (type V autotransporter secretion mechanism). Serine protease autotransporters of Enterobacteriaceae (SPATEs) include adhesins, toxins, and proteases that can contribute to the virulence. Plasmid-encoded toxin (Pet) is the predominant protein in culture supernatants of enteroaggregative E. coli prototype strain 042 and has been extensively studied. Pet toxin is encoded on the 65-MDa adherence-related plasmid of EAEC 042 strain. In this work, Pet protein was found in the supernatant obtained from Proteus mirabilis RTX339 strain isolated from a psychiatric patient suffering complicated urinary tract infections (UTIs). The nucleotide sequence of pet gene was obtained using primers designed from E. coli 042 pet gene reported. The alignment of the sequence showed 100% identity with the pet gene reported. Is important to note that Proteus mirabilis RTX339 pet gene has chromosomal location. The chromosomal location of the gene was established since no plasmids were harbored by this strain.
文摘Climate change impacts on water resources are expected to be significant in Yemen. Efforts have been made to understand the expected changes and develop mitigation possibilities for the expected scenarios for a future sustainable use of resources and mitigation of expected impacts. The paper describes the development of a detailed baseline database and the assessment of climate change and variability impacts on water resources over the 2030, 2050 and 2080 time horizon on a Yemen-wide scale. Based on downscaled Global Climate Model data, a range of scenarios were established, representing potential Mean, Warm ＆ Wet and Hot ＆ Dry conditions as derived by evaluating worst case scenarios from the ensemble of the global models for the specified years. The results of the model include an estimated runoff coefficient, monthly rainfall, runoff, infiltration and evaporation representing the water balance in the different catchments. Analysis of the different evaluated scenarios shows that in the Mid, Warm and Wet scenario the hydrological components are generally higher than in the baseline scenario. For the Hot ＆ Dry scenario, runoff, infiltration and evapotranspiration are decreasing due to the decreasing precipitation and increase in temperature. The relative changes in runoff are strongest.
文摘The spate of deadly mass shootings in the United States have caused concern both domestically and in the international community. According to a report by the China Society for Human Rights Studies, a member of the United Nations Conference of Non-Governmental Organizations, the rampant gun violence reflects the profound crisis in the U.S. political and social systems and highlights the U.S. hypocrisy on human rights.
