Layer chicken microbiota:a comprehensive analysis of spatial and temporal dynamics across all major gut sections

Background The gut microbiota influences chicken health,welfare,and productivity.A diverse and balanced microbiota has been associated with improved growth,efficient feed utilisation,a well-developed immune system,disease resistance,and stress tolerance in chickens.Previous studies on chicken gut microbiota have predominantly focused on broiler chickens and have usually been limited to one or two sections of the digestive system,under con-trolled research environments,and often sampled at a single time point.To extend these studies,this investigation examined the microbiota of commercially raised layer chickens across all major gut sections of the digestive system and with regular sampling from rearing to the end of production at 80 weeks.The aim was to build a detailed picture of microbiota development across the entire digestive system of layer chickens and study spatial and temporal dynamics.Results The taxonomic composition of gut microbiota differed significantly between birds in the rearing and pro-duction stages,indicating a shift after laying onset.Similar microbiota compositions were observed between proven-triculus and gizzard,as well as between jejunum and ileum,likely due to their anatomical proximity.Lactobacil-lus dominated the upper gut in pullets and the lower gut in older birds.The oesophagus had a high proportion of Proteobacteria,including opportunistic pathogens such as Gallibacterium.Relative abundance of Gallibacterium increased after peak production in multiple gut sections.Aeriscardovia was enriched in the late-lay phase compared to younger birds in multiple gut sections.Age influenced microbial richness and diversity in different organs.The upper gut showed decreased diversity over time,possibly influenced by dietary changes,while the lower gut,specifi-cally cecum and colon,displayed increased richness as birds matured.However,age-related changes were inconsist-ent across all organs,suggesting the influence of organ-specific factors in microbiota maturation.Conclusion Addressing a gap in previous research,this study explored the microbiota across all major gut sections and tracked their dynamics from rearing to the end of the production cycle in commercially raised layer chickens.This study provides a comprehensive understanding of microbiota structure and development which help to develop targeted strategies to optimise gut health and overall productivity in poultry production.

Spatial compartmentalization and temporal stability of associated microbiota in Pacific oyster Crassostrea gigas

The Pacific oyster Crassostrea gigas,one of the most exploited molluscs in the world,has suffered from massive mortality in recent decades,and the occurrence mechanisms have not been well characterized.In this study,to reveal the relationship of associated microbiota to the fitness of oysters,temporal dynamics of microbiota in the gill,hemolymph,and hepatopancreas of C.gigas during April 2018-January 2019 were investigated by 16 S rRNA gene sequencing.The microbiota in C.gigas exhibited tissue heterogeneity,of which Spirochaetaceae was dominant in the gill and hemolymph while Mycoplasmataceae enriched in the hepatopancreas.Co-occurrence network demonstrated that the gill microbiota exhibited higher inter-taxon connectivity while the hemolymph microbiota had more modules.The richness(Chao 1 index)and diversity(Shannon index)of microbial community in each tissue showed no significant seasonal variations,except for the hepatopancreas having a higher richness in the autumn.Similarly,beta diversity analysis indicated a relatively stable microbiota in each tissue during the sampling period,showing relative abundance of the dominant taxa exhibiting temporal dynamics.Results indicate that the microbial community in C.gigas showed a tissue-specific stability with temporal dynamics in the composition,which might be essential for the tissue functioning and environmental adaption in oysters.This work provides a baseline microbiota in C.gigas and is helpful for the understanding of host-microbiota interaction in oysters.

Spatial and temporal variation of water clarity in typical reservoirs in the Beijing-Tianjin-Hebei region observed by GF 1-WFV satellite data

Rapidly monitoring regional water quality and the changing trend is of great practical and scientific significance,especially for the Beijing-Tianjin-Hebei(BTH)region of China where water resources are relatively scarce and inland water bodies are generally small.The remote sensing data of the GF 1 satellite launched in 2013 have characteristics of high spatial and temporal resolution,which can be used for the dynamic monitoring of the water environment in small lakes and reservoirs.However,the water quality remote sensing monitoring model based on the GF 1 satellite data for lakes and reservoirs in BTH is still lacking because of the considerable differences in the optical characteristics of the lakes and reservoirs.In this paper,the typical reservoirs in BTH-Guanting Reservoir,Yuqiao Reservoir,Panjiakou Reservoir,and Daheiting Reservoir are taken as the study areas.In the atmospheric correction of GF 1-WFV,the relative radiation normalized atmospheric correction was adopted after comparing it with other methods,such as 6 S and FLAASH.In the water clarity retrieval,a water color hue angle based model was proposed and outperformed other available published models,with the R 2 of 0.74 and MRE of 31.7%.The clarity products of the four typical reservoirs in the BTH region in 2013-2019 were produced using the GF 1-WFV data.Based on the products,temporal and spatial changes in clarity were analyzed,and the main influencing factors for each water body were discussed.It was found that the clarity of Guanting,Daheiting,and Panjiakou reservoirs showed an upward trend during this period,while that of Yuqiao Reservoir showed a downward trend.In the influencing factors,the water level of the water bodies can be an important factor related to the water clarity changes in this region.

Spatial-temporal heterogeneity and controlling factors of evapotranspiration in Nujiang River Basin based on Variable Infiltration Capacity(VIC)model

Land surface evapotranspiration(ET)is a critical component in the hydrological cycle but has not well been understood in data-scarce areas especially in river basins,like Nujiang River(NRB)which is characterized by large elevation gradient and different vegetation zones with complex processes of water and energy exchange.The quality of ET from optical remote sensing is constrained by cloud cover which is common in the NRB in the monsoon seasons.To understand factors controlling the spatial-temporal heterogeneity of ET in NRB,we employed the Variable Infiltration Capacity(VIC)hydrological model by parameter optimization with support of quality controlled remote sensing ET product and observed river runoff series in the river.The modeled ET has increased during 1984-2018,which might be one of the reasons for the runoff decrease but precipitation increase in the same period.ET increase and runoff decrease tended to be quicker within altitudinal band of 2000-4000 m than in other areas in NRB.We observed that ET variation in different climatic zones were controlled by different factors.ET is generally positively correlated with precipitation,temperature,and shortwave radiation but negatively with relative humidity.In the Tundra Climate(Et)zone in the upper reach of NRB,ET is controlled by precipitation,while it is controlled by shortwave radiation in the snow climate with dry winter(Dw)zone.ET increase is influenced by the increase of temperature,wind speed,and shortwave radiation in the middle and downstream of NRB with warm temperate climate,fully humid(Cf)and warm temperate climate with dry winter(Cw).

Temporal and spatial differences in human activities performed in Urban Green Spaces of Vilnius(Lithuania)

Urban green spaces(UGS)are relevant to city well-being,as recognized by the United Nations’Sustainable Development Goals(SDGs).However,few studies have studied the temporal use of UGS.This work assessed the seasonal,weekly,and daily use of three urban green spaces(Vingis Park,Bernardino Garden,and Jomantas Park)in Vilnius(Lithuania).The study is based on an on-site observation-based survey,which recorded users’characteristics,activities,and weather conditions during summer and winter.The results showed that UGS’s seasonal,weekly,and daily use differed according to park and users’characteristics.Parks with a higher diversity of facilities had a high seasonal difference in the number of observed activities.User numbers were higher in the summer for activities with children,social activities,sports,and water activities than in the winter.Jomantas Park had the lowest variability in user characteristics.Weather variables were linked to changes in users’activities.Higher precipitation and lower temperature were associated with reducing the number of users and the diversity of registered activities.Most of the stationary activities were observed during summer.The diversity of the observed activities was associated with the available facilities rather than the park size.The distribution of stationary activities was spatially correlated with facility/equipment(benches,playgrounds,sports,and fitness equipment)and proximity to water features.The results of this study are relevant for UGS design,planning,and management.

Temporal and Spatial Dynamics of Vaccine-Derived Poliovirus (VDPV) in Democratic Republic of Congo from 2018 to 2023

Background: The Democratic Republic of Congo (DRC) has been facing outbreaks of VDPV since 2017. These wild poliovirus variants are responsible for poliomyelitis, which is in the process of eradication.. In the following lines, we try to show the evolution of VDPV cases across the country in order to understand their chronological dynamics and seasonal influence. Methods: We conducted a cross-sectional study of of VDPV notified in the DRC from 2018 to 2023. Maps of the spatial dynamics of VDPV cases were produced from attack rates with QGIS® (3.22.8). As for temporal dynamics, time series were decomposed and presented in the form of graphs showing the chronological evolution of VDPV cases and their seasonal trend, using R.4.0 software package. Results: A total of 1196 Cases of VDPV types 1, 2 and 3 were recorded in the biological confirmation databases of the INRB and the Expanded Program of Immunization during the study period across25 provinces. The eastern part of the country reporting the most cases. The general trend is upwards, with a peak in 2022 of 527 cases, whereas in 2021 there was a notable drop of 31 cases. Analysis of the temporal breakdown suggests a seasonal pattern, with peaks between the months of September and December, considered being rainy periods in some provinces. Conclusion: During the 6 years of our study (2018 - 2023) almost all the Health Zones were hit by VDPV epidemics. The eastern part was the most impacted. The seasonal component is well marked suggesting a rise in detection in the rainy season and during pivotal periods of climate change.

Spatial and Temporal Distribution Characteristics of Snowstorm in Ulanqab City in Recent 30 Years

Based on the data of daily snowfall and weather phenomena of 11 national meteorological stations in Ulanqab City from 1991 to 2020,the spatial and temporal distribution characteristics of snowstorm were analyzed.The results show that the snowstorm in Ulanqab had obvious seasonal distribution characteristics,mainly happening in spring(March-May)and autumn(September-November).It also had obvious regional distribution in space,and the snowstorm center appeared in Chahar Right Wing Middle Banner and Jining District,namely the east side of the Yinshan Mountain.In the past 30 years,the amount of snowstorm in the whole year in Ulanqab showed a certain fluctuation trend,and the number of snowstorm days had shown an obvious upward trend since 2011.

Study on the Spatial and Temporal Distribution of Blue Algae in Lake Dianshan in Summer

To understand the spatial and temporal variation characteristics of blue algae in summer in Lake Dianshan,the phytoplankton in Lake Dianshan from June to September in 2009 was surveyed. It found 11 genera and 28 species blue algae in total. Microcystis,Oscillatoria and Chroococcus were the main composition communities of blue algae in Lake Dianshan in summer. In the survey period,the average density of blue algae in Lake Dianshan was 16.48×106 cells/L which changed during 1.01×106-59.76×106 cells/L. The characteristics were:September > July > August > June. The mass propagation and aggregation of Microcystis in September caused that the water blooms phenomenon in the partial water areas was serious. In the space,the average density of blue algae in the west and southwest parts of Lake Dianshan was bigger than in the east and southeast. When the nutritive matter was sufficient,the temperature was the main factor which affected the generation and disappearance of blue algae water blooms. The wind direction was also an important factor which affected the distribution of blue algae.

Analysis of the Spatial and Temporal Characteristics of Extreme Precipitation Events in Liaoning Province

Daily precipitation amounts from 1961 to 2005 in 35 observation stations in Liaoning Province were selected in order to study the temporal and spatial distribution of extreme precipitation events.By dint of EOF,REOF,mean-square-error and other ways,the changes in different regions of extreme precipitation and distribution were reflected.The analysis showed that,extreme precipitation in Liaoning Province could be divided into three areas,which were western Liaoning mountains and parts of northern areas,eastern Liaoning mountainous,near-coastal areas of Liaohe River Plain.In the relatively large precipitation areas,extreme precipitation threshold was also higher,and vice versa.The lower frequency of extreme precipitation events had a greater contribution to total precipitation;extreme precipitation,total precipitation and total rain days had the greatest changes in the summer,and the least changes in the winter;number of days of extreme precipitation changes in each season were not great;the change of extreme precipitation was not obvious in the long term.

Temporal and Spatial Variation of Surface Albedo in Tibetan Plateau during 1982-2000

[Objective] Temporal and spatial variation of surface albedo in Tibetan Plateau were studied in our paper.[Method] Based on NOAA/AVHRR data,different algorithms were used to retrieve surface albedo in Tibetan Plateau,and it showed that the result of Stroeve was mostly close to observed data.Based on retrieval algorithm from Stroeve,the spatial distribution of surface albedo in Tibetan Plateau was obtained by means of NOAA/AVHRR data in 1982-2000.[Result] The distribution of annual mean surface albedo in Tibetan Plateau was identical with that of geographical zone in plateau area;annual mean surface albedo in plateau area showed slight decrease trend which was different in various regions;monthly surface albedo in plateau area had obviously zonal distribution and changed with time evidently.[Conclusion] Our study will be helpful to improving the parameterization scheme of surface albedo in climate model,revealing the internal mechanism of local and regional climate change and enhancing the level of long-term climate forecast.

Analysis on the Temporal and Spatial Change of Grain Production of China in Recent Years and Its Adaptation Strategies

A comprehensive analysis on the change of the total grain production and the temporal and spatial change of three main crops production（including wheat,maize and rice）,as well as the transfer trace of the center gravity of grain production in China were analyzed to reveal the overall developing trend of the grain production,explore the reasons and finally propose the corresponding suggestions and strategies to cope with the situation.

Temporal and Spatial Variation Characteristics of Precipitation during Crop Growing Season in Northeast China

[Objective] The aims were to understand variation characteristics of water resources and provide theoretical guidance for the formulation of agricultural irrigation methods.[Method] Taking the precipitation records during crop growing season（from April to September）observed by 177 weather stations from 1971 to 2008 in the three provinces of Northeast China（Heilongjiang,Jilin and Liaoning）as research data,annual change and spatial distribution characteristics of precipitation during crop growing season were analyzed by means of small grid interpolation and climatic trend rate.[Result] The precipitation during crop growing season general exhibited the decreasing trend and the precipitation trend rate was-8.6 mm/10a in Northeast China.In addition,there was lack of rain from 1971 to 1980 and relatively abundant of rain during 1981 and 1990 respectively.Moreover,the precipitation obviously exhibited decreasing trend from 1991 to 2008.But the decreasing trend was inconsistent in spatial distributions,that was,the precipitation slightly increased in relatively rainless areas and obviously decreased in relatively rainy areas.[Conclusion] The areas with obvious decreasing trend of precipitation during crop growing season are the main grain producing zones in Northeast China,so the problem of food production security caused by the precipitation changes should be paid enough attention.

Spatial-temporal Evolvement Characteristics of Climate Productivity for the Plants on Inner Mongolia Desert Steppe

Thornthwaite Memorial model and other statistic methods were used to calculate the climate-productivity of plants with the meteorological data from 1961 to 2007 at 9 stations distributed on Inner Mongolia desert steppe.The spatial and temporal variation characteristics of climate-productivity were analyzed by using the methods of the tendency rate of the climate trend,accumulative anomaly,and spatial difference and so on.The results showed that the climate-productivity kept linear increased trend over Inner Mongolia desert steppe in recent 47 years,but not significant.In spatial distribution,the climate-productivity reduced with the increased latitude.The climate-productivity in southwest part of Inner Mongolia desert steppe was growing while that in the southeast was reducing.The variation rate of the climate-productivity increased from the northwest part to the southeast part of Inner Mongolia desert steppe.In recent 47 years,the climate-productivity in southeast Jurh underwent the greatest decreasing extent,and the region was the sensitive area of the climate-productivity variation.

Temporal and Spatial Characteristics of Rainstorm during the Crops Growth Period in North China Region

By using the daily precipitation data from 1961 to 2005 in North China region,the temporal and spatial distribution characteristics of rainstorm process occurrence and the rainstorm intensity during the crops growth period were studied.The results showed that the rainstorm intensity and the rainstorm process during the crops growth period in North China region both had the obvious annual fluctuations and era variation characteristics.Although the rainstorm and heavy rainstorm occurred in North China region every year,the annual variations were great,and the variation coefficients respectively reached 36.9% and 53.1%.The torrential rain occurred once in every 4-5 years,and the rainstorm process occurred once in every 11 years.Although the torrential rain and rainstorm process occurred in fewer years,their annual fluctuations were more obvious.The peak value zones of rainstorm intensity which was greater and the rainstorm process which occurred frequently were in the 1960s.After 1999,the rainstorm intensity and the rainstorm process were in low value zone of historical stage from 1961 to 2005.Moreover,the 1970s-1990s was between high value and low value,and the rainstorms in different intensities which weren't synchronous happened in the period.In addition,the spatial distribution of annual average rainstorm days presented the tendency which increased obviously from northwest to southeast in Northern China,and the variation coefficient of rainstorm days presented the tendency which increased gradually from southeast to northwest.Generally,the more the annual average rainstorm days are,the smaller the variation coefficient is,and vice versa.The statistics results also showed that precipitation in North China had obvious positive correlation relationship with the rainstorm days.

Analysis on Temporal and Spatial Characteristic and Variation of Precipitation in Ningxia Hui Autonomous Region

Based on seasonal and annual observed precipitation data at 19 meteorological stations in Ningxia Hui Autonomous Region,the temporal and spatial distribution characteristic is analyzed by EOF method.And spatial characteristics and temporal changes trends of EOF of seasonal and annual precipitation have been showed.The main period and sudden changes point of EOF of annual precipitation have been studied by Mann-Kendall and spectrum analysis method.

Temporal and Spatial Variation and Distribution Characteristics of Maximum and Minimum Temperature from 1971 to 2008 in Tibet

Based on temperature data of meteorological stations from 1971 to 2008 in Tibet,the temporal and spatial variation of maximum andminimum temperature in Tibet was analyzed.The results showed that both maximum temperature andminimum temperature increased distinctly,the warming amplitude of winter was the highest among the four seasons,and next came spring.The increment ofminimum temperature was visibly over that of maximum temperature,particularlyminimum temperature in winter with significant increment.For spatial variation,maximum temperature in most stations increased except particular stations,while theminimum temperature in all stations rose.In addition,the space variation law ofminimum temperature,being more obvious thanminimum temperature,increased from southeast to northwest with different spatial changes in various seasons.From decadal variation,both maximum andminimum temperature appeared increase from 1970s to the first eight years in the 21st century,and the rise ofminimum temperature was significant greater than maximum temperature.The increase of maximum andminimum temperature was the highest from 2001 to 2008,whereas the lowest in 1970s.

Spatial and Temporal Differences of Urban Land Comprehensive Carrying Capacity in Guangxi

The comprehensive carrying capacity of urban land can reflect the re- source level, economic scale, social development and environmental pressure of ur- ban land carrying. The assessment indicator system of urban land comprehensive carrying capacity was constructed from the 4 aspects of resource, economy, society, environment, and principal component analysis and cluster analysis were used to evaluate the urban land comprehensive carrying capacity of Guangxi and the 14 cities in 2005-2014, and analyzed its spatial and temporal characteristics as well as the driving forces, with the aim to provide references for improving the urban land comprehensive carrying capacity. The results showed that, the overall urban land comprehensive carrying capacity in Guangxi increased in 2005-2014, and there were significant differences in the land comprehensive carrying capacities among the cities in Guangxi in 2005-2014, in which Liuzhou, Guilin, Nanning belonged to the regions with the highest carrying capacity, while Beihai, Yulin, Wutong belonged to the regions with high carrying capacity, and the carrying capacities of the other cities changed with the changes of time. The economic development degree was an important factor influencing urban land comprehensive carrying capacity, but could not directly represent the urban land comprehensive carrying capacity level.

The Temporal and Spatial Evolution Characteristics Analysis of Jiangsu Tourism Economy

[Objective] The paper aimed to reflect the spatial pattern and temporal and spatial evolution characteristics, the differences between inter-regional tourism economy was measured from the quality aspect, which provided a reference for the local governments in the future tourism development. [Method] Using the location entropy methods, three time periods side of tourism-related date of 2000, 2005, 2007 were selected, from the angle of the spatial pattern and the evolution of the differences within the different scales, the spatial and temporal evolution characteristics of the economic development level of Jiangsu were analysed. [Result] The results showed that from the aspect of spatial evolution pattern, as time goes on, the economic development of Jiangsu tourism has experienced morphological evolution of concentration- dispersion decrease-stability; when it comes to the development of the tourism economy, in recent years, the overall gap between the tourism economy in Jiangsu did not widen, the gap mainly led by the region one after another. According to their volatility, it will be divided into four categories: A Stable type (Wuxi, Xuzhou, Lianyungang and Taizhou), B Increasing type (Huai’an), C Fluctuations type (Nanjing, Changzhou, Suzhou and Yangzhou) and D Depression type (Nantong, Yancheng, Zhenjiang and Suqian). [Conclusion] Location entropy was quoted into tourism economic analysis, the method was simple and easy to understand, the result was accurate and convincing, which provided a reference for travel economic development and investment decision-making of Jiangsu.

Analysis on Features of Spatial and Temporal Distribution of Thunderstorm in Shanghai

Based in 11 daily weather observation station data in Shanghai from 1971 to 2008,a careful research and analysis on the features of thunderstorms spatial and temporal distribution and thunderstorm movement in Shanghai was carried out by using the statistical software of SAS,the method of Mann-Kendall test and wavelets. The results showed that the average annual numbers of thunderstorms days were 26.1,and inter-annual thunderstorm variability was obvious,the annual number of thunderstorm days had a decreasing trend,its value of decreasing days was about-0.418 5 d/10 a. Mann-Kendall test showed that there was an abrupt change in 2000. The seasonal variation of thunderstorm in Shanghai was explicit. The period from March to September was the season when thunderstorm occurred most frequently,about 64.9% of the thunderstorms in a year took place in summer. The results from wavelets analysis showed that the variation cycle period of the annual number of thunderstorms days was about 3,5,12 and 20 years.

Study on the Temporal and Spatial Distribution Characteristic of Acid Rain in Guangxi Based on EOF Analysis

Based on acid rain data from ten monitoring sites in Guangxi from 2003 to 2009,the temporal and spatial distribution characteristic of acid rain in Guangxi were analyzed by means of empirical orthogonal function resolution(EOF).The results showed that there was fluctuating change of acid rain frequency in Guangxi,and acid rain pollution became severer in 2004-2008;acid rain frequency changed conformably in the whole region and it was obviously higher in eastern and northwestern Guangxi,while acid rain pollution became severe in western Guangxi;acid rain frequency varied out of phase between northeastern and southwestern Guangxi in an individual year.