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A review on rules for examination of licensing criteria for producing foods for special medical purpose in China 被引量:9
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作者 Lei Wu Lantian Zhang Yan Zhang 《Food Science and Human Wellness》 SCIE 2019年第2期106-114,共9页
In this paper,the background,principles,and main contents of the rules for the examination of production licenses of foods for special medical purposes are introduced.The contents including general rules,production si... In this paper,the background,principles,and main contents of the rules for the examination of production licenses of foods for special medical purposes are introduced.The contents including general rules,production sites,equipment facilities,equipment layout,technological process,personnel management,management system and supplementary rules are interpreted item by item.This study has important value and historical significance for promoting the development of Chinese enterprises and industries of foods for special medical purposes. 展开更多
关键词 Review foods for special medical purposes Criteria of production Examination of licensing
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Using the Delphi method to propose foods for special medical purposes health effect evaluation indicators
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作者 Peng Ren Hai-Yue Wang Zeng-Ning Li 《Journal of Nutritional Oncology》 2023年第1期47-52,共6页
Objective:The Delphi method was used to propose health effect evaluation indicators to assess foods for special medical purposes(FSMPs).This lays the foundation for the formation of a big data model for human health t... Objective:The Delphi method was used to propose health effect evaluation indicators to assess foods for special medical purposes(FSMPs).This lays the foundation for the formation of a big data model for human health testing,as well as a big data platform for the health and safety evaluation of special medical foods.Methods:The Delphi method was used to conduct two rounds of expert consultation on the constructed FSMP health effect evaluation indicators.Results:Ten major items were identified after two rounds of expert consultation.Among these,there were 10 primary entries,32 secondary entries,50 tertiary entries,and 28 quaternary entries.Conclusion:The complete list of evaluation indicators contains 10 entries,which can comprehensively and systematically monitor adverse reactions to the use of FSMPs.The present findings lay the foundation for a big data platform to evaluate the health and safety of special foods. 展开更多
关键词 food for special medical purpose(FSMP) Delphi Health effect Expert consultation Evaluation indicators
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Mathematical rules for synergistic,additive,and antagonistic effects of multi-drug combinations and their application in research and development of combinatorial drugs and special medical food combinations 被引量:3
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作者 Shoujun Yuan Haoyu Chen 《Food Science and Human Wellness》 SCIE 2019年第2期136-141,共6页
Multi-drug(or multi-element)combinations are often prescribed in the practice of clinical medicine and as foods for special medical purposes.The main motivations for these combinations are that most diseases contain m... Multi-drug(or multi-element)combinations are often prescribed in the practice of clinical medicine and as foods for special medical purposes.The main motivations for these combinations are that most diseases contain multiple related targets and an appropriate combination can maximize benefits while minimizing adverse reactions.As such,it is especially important to derive mathematical models for their quantitative calculation.In this paper,we introduce mathematical rules for the synergistic,additive,and antagonistic effects of multi-drug combinations developed in our laboratory.We have established a“onebelt,one-line”model and provide examples of the quantitative calculation of the synergistic,additive,and antagonistic effects of a combination of multiple components.We also explain how to scientifically and precisely determine the intensity of these synergies,additions and antagonisms,as well as their corresponding dose ranges,thereby laying a solid theoretical foundation for market listing combinatorial drugs and foods for special medical purposes. 展开更多
关键词 Multi-drug combination Dose-effect belt One-belt one-line model foods for special medical purposes Synergy Addition ANTAGONISM
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New Food Safety Law of China and the special issue on food safety in China 被引量:1
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作者 Shu Geng LIU Xu Roger Beachy 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2015年第11期2136-2141,共6页
1. IntroductionThere have been a number of serious incidents of compro- mised food safety in China in recent years. To list a few, these include: a Salmonella outbreak, the use of horse- meat in burgers, the illegal ... 1. IntroductionThere have been a number of serious incidents of compro- mised food safety in China in recent years. To list a few, these include: a Salmonella outbreak, the use of horse- meat in burgers, the illegal use of phthalates as a clouding agent, the contamination of formula milk by melamine, the meat scandal of Shanghai Husi Food Company which sold reprocessed stale meat to many fast food chains includ- ing McDonald's, Burger King, and KFC across theworld, 展开更多
关键词 New food Safety Law of China and the special issue on food safety in China FSMA
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Impact of Africa Regional Food Security Program on Nigerian Farmers: The Case of National Special Program for Food Security 被引量:4
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作者 P. O. Agbola S. A.Yusuf +1 位作者 A. A. Adubi D. O. Awotide 《Journal of Agricultural Science and Technology》 2010年第5期51-60,共10页
This paper examined the impact of National Special Program for Food Security (NSPFS) on the beneficiaries through a qualitative measurement of the food security status of the beneficiaries. A food insecurity index w... This paper examined the impact of National Special Program for Food Security (NSPFS) on the beneficiaries through a qualitative measurement of the food security status of the beneficiaries. A food insecurity index was estimated to measure the well-being of the beneficiaries as affected by the impact of the program. Beneficiaries were classified into four categories: food secure (41%), food insecure without hunger (46%), food insecure with moderate hunger (11%), and food insecure with severe hunger (2%). Perceptions of beneficiaries were measured. In conclusion, the program has had a positive impact and enhanced food security among the Nigerian beneficiaries. It is not without its short comings which include delayed and untimely disbursement of funds and lack of output markets, irrigation facilities, production inputs and adequate training on technology know-how. The default rate and number of debtors remain high among beneficiaries. Efforts should be made to correct the short comings to improve the effectiveness of the program and justify the huge financial resources that have been committed. 展开更多
关键词 National special program for food security BENEFICIARIES Nigeria.
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The Preparation Technology Research and Composition Analysis of Anti-fatigue Yikangshu Granules
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作者 Kun TANG Tong LIN +2 位作者 Chundan ZHANG Yanxia LU Shunxing GUO 《Medicinal Plant》 CAS 2021年第5期46-50,共5页
[Objectives]To optimize the preparation technology of Yikangshu granule,an anti-fatigue health food.[Methods]The process was studied from three aspects(raw material extraction,excipient selection and forming process),... [Objectives]To optimize the preparation technology of Yikangshu granule,an anti-fatigue health food.[Methods]The process was studied from three aspects(raw material extraction,excipient selection and forming process),and then it was verified in pilot production,and the content of the three components of the product was determined.[Results]The optimum production process of the formula granules was as follows:raw materials were extracted(adding 1∶9 water,extracting twice,2.5 h each time);vacuum concentration;adding excipients and mixing after vacuum drying,crushing and sieving;granulation of 75%ethanol sifted by a 20-mesh sieve;drying at 60℃,sifting through 14-mesh,and packaging.The average content of products obtained was as follows:crude polysaccharides(3204 mg/100 g),total saponins(2295 mg/100 g),astragaloside A(21 mg/100 g).[Conclusions]The optimized preparation technology is stable and feasible,and has a quality that can be controlled,which can provide a reference for the development of health food in food industry. 展开更多
关键词 anti-fatigue Health food Formula granules Technology research Composition analysis
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Food Safety Detection Methods Applied to National Special Rectification of Product Quality and Food Safety
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《China Standardization》 2007年第6期35-,共1页
  Afour-month period of national special rectification for product quality and food safety officially started on August 25, and was focused on eight fields, including those of agricultural products and processed foo...   Afour-month period of national special rectification for product quality and food safety officially started on August 25, and was focused on eight fields, including those of agricultural products and processed foods.…… 展开更多
关键词 food Safety Detection Methods Applied to National special Rectification of Product Quality and food Safety
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Housing pattern and food habit of the Mro-tribe community in Bang-ladesh: A forest dependence perspective 被引量:1
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作者 Mohammad Shaheed Hossain Chowdhury Md.Danesh Miah 《Journal of Forestry Research》 SCIE CAS CSCD 2003年第3期253-258,共6页
An exploratory study was conducted on the housing pattern and food habit of the Mro tribe in Bandarban region, Bangladesh, highlighting their indigenous knowledge. The study was carried out with respect to three incom... An exploratory study was conducted on the housing pattern and food habit of the Mro tribe in Bandarban region, Bangladesh, highlighting their indigenous knowledge. The study was carried out with respect to three income groups. A total of 36 households (12 from each income groups) were assessed using different participatory appraisals through semi-structured questionnaires. A special type of indigenous knowledge on housing pattern and food habit was explored in the Mro community, which correspond to the severe dependence on forest resources. 展开更多
关键词 Housing pattern special and Favorite food Cooking and drinking procedure MRO Bangladesh
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Direct Marketing and Consumer Trust in Organic Food Products: Vilnius (Lithuania) Case
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作者 Virgilijus Skulskis Vilija Girgzdiene 《Journal of Agricultural Science and Technology(B)》 2013年第4期272-283,共12页
The market of organic food products has a continuous upward trend though the growth rate and sale channels in individual countries are rather different. This research aims to identify the key factors that influence co... The market of organic food products has a continuous upward trend though the growth rate and sale channels in individual countries are rather different. This research aims to identify the key factors that influence consumers' choice to buy organic food products in Lithuania focusing on the consumer trust in the quality and the most acceptable marketing channels. In order to get the necessary information, the residents of Vilnius (the capital of Lithuania), as having the greatest potential for the organic food market in the country, were interviewed. The analysis of collected data was done using the methods of mathematical statistics and comparison of relative frequencies. The results of this research prove the increasing consumers' interest in organic food products. The majority of consumers consider these products to be healthier, better-tasting and fresher. Only about a third of the purchasers (usually buying frequently) can see differences between organic and non-organic products, therefore, there is a problem to distinguish organic food from conventional products and, consequently, remains a possibility to cheat the consumer. An additional quality control and direct sales from known and familiar farmers might increase trust in the quality of organic food products. So far, supermarkets are the most important place to buy organic foods, however, the greater consumer confidence in the quality of organic products bought at farmers' markets and the acceptance of the majority of purchasers to buy organic food products directly from the farmers suppose the need to develop a direct marketing system. 展开更多
关键词 Organic food products CONSUMERS consumer trust direct marketing specialized farmers' markets.
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中国特殊医学用途配方食品发展历程 被引量:1
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作者 冯一 蔡威 《临床儿科杂志》 CAS CSCD 北大核心 2024年第2期93-95,共3页
特殊医学用途配方食品(FSMP)又称特医食品,在中国的发展历程中,经历了从无到有,从引进到自主研发的转变。本文梳理了中国特医食品的发展脉络,探讨其在临床应用、产品种类、研发能力以及法规标准管理等方面的进展,我们也可以看到中国特... 特殊医学用途配方食品(FSMP)又称特医食品,在中国的发展历程中,经历了从无到有,从引进到自主研发的转变。本文梳理了中国特医食品的发展脉络,探讨其在临床应用、产品种类、研发能力以及法规标准管理等方面的进展,我们也可以看到中国特医食品行业面临的挑战和机遇。随着国内外市场的不断扩大和技术的不断进步,特医食品将在临床营养治疗领域发挥越来越重要的作用。 展开更多
关键词 特殊医学用途配方食品 临床营养 法规标准
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农业生产性服务与粮食生产韧性:影响机制与实证检验
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作者 朱满德 张青 《湖南农业大学学报(社会科学版)》 CSSCI 2024年第6期1-11,共11页
以抵抗力、适应力、变革力三个维度为基础构建粮食生产韧性指标体系测度中国及各省域的粮食生产韧性,并利用2011—2022年省级面板数据和双向固定效应模型实证检验农业生产性服务发展对粮食生产韧性的影响效应及其作用机制,结果表明:中... 以抵抗力、适应力、变革力三个维度为基础构建粮食生产韧性指标体系测度中国及各省域的粮食生产韧性,并利用2011—2022年省级面板数据和双向固定效应模型实证检验农业生产性服务发展对粮食生产韧性的影响效应及其作用机制,结果表明:中国粮食生产韧性呈逐年提升态势,但省际间分异明显;农业生产性服务可以显著促进粮食生产韧性,其中规模化效应和专业化效应是主要的作用机制;分维度实证表明,农业生产性服务能显著提升粮食生产韧性的抵抗力和变革力,但对适应力起到一定程度的抑制作用;异质性分析显示,农业生产性服务在北方省份的促进作用要大于南方省份,在东部地区的促进作用比中西部地区更加显著。 展开更多
关键词 农业生产性服务 粮食生产韧性 规模化效应 专业化效应
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案例教学在专业学位研究生“食品加工与贮运专题”课程中的应用
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作者 朱明明 李慧杰 +3 位作者 焦凌霞 高晗 师玉忠 马长明 《农产品加工》 2024年第18期138-141,145,共5页
“食品加工与贮运专题”是食品加工与安全专业硕士的一门核心课程,其教学改革对于培养应用型食品硕士人才至关重要。在“食品加工与贮运专题”课程教学过程中,适宜地引入师生熟悉的、与食品生产和贮藏相关的、关注学科发展前沿的代表性... “食品加工与贮运专题”是食品加工与安全专业硕士的一门核心课程,其教学改革对于培养应用型食品硕士人才至关重要。在“食品加工与贮运专题”课程教学过程中,适宜地引入师生熟悉的、与食品生产和贮藏相关的、关注学科发展前沿的代表性案例,能最大限度地激发学生学习热情,鼓励其探索并解决实际问题。这种教学模式有利于研究生深入思考与食品加工及贮藏工艺相关的问题,并通过讨论获取解决办法,掌握重难点,真正实现理论知识的扩展和迁移。同时,任课教师应从兼顾传统教学、考虑学生适应性、注重总结归纳等方面,关注案例教学融入课堂的可行性,对教学模式、内容及考核方式进行改革,以达到课堂教学与食品生产过程的有机结合,提高“食品加工与贮运专题”课堂教学效果,引导学生树立正确的责任观和价值观。 展开更多
关键词 食品加工与贮运专题 案例教学 应用型人才 实践 教学改革
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百合鳞茎营养素与植物化学成分研究进展 被引量:1
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作者 米璐 林玉红 +1 位作者 廖小军 徐贞贞 《食品工业科技》 CAS 北大核心 2024年第14期1-14,共14页
百合是多年生草本植物,兼具观赏、食用和药用价值,其可食用或药用部位一般为鳞茎。近年来,百合鳞茎作为一种特色蔬菜,受到消费者青睐。本文重点从不同品种视角对百合鳞茎进行了分析,首先归纳了碳水化合物(包括活性多糖)、蛋白质及氨基... 百合是多年生草本植物,兼具观赏、食用和药用价值,其可食用或药用部位一般为鳞茎。近年来,百合鳞茎作为一种特色蔬菜,受到消费者青睐。本文重点从不同品种视角对百合鳞茎进行了分析,首先归纳了碳水化合物(包括活性多糖)、蛋白质及氨基酸、脂类、矿物质和维生素等营养素的详细构成,进一步总结了其甾体皂苷(包括螺甾烷醇、异螺甾烷醇、变形螺甾烷醇、呋甾烷醇类)、酚酸类(包括单聚酚酸、酚酸甘油酯和苷类)、黄酮类(包括黄酮醇、黄烷醇、查尔酮、黄烷酮、黄烷酮醇、花色苷类等)和生物碱类(包括异喹啉、吡咯啉、哌啶和甾体糖苷类)等已知植物化学成分的构成及其生物活性,最后对百合在保健食品中的应用进行概括,以期为百合研究及非药用加工利用提供理论依据和研究思路。 展开更多
关键词 百合 百合鳞茎 特色蔬菜 植物化学成分 生物活性 功能性食品
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特殊医学用途配方食品产业的发展及治理 被引量:1
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作者 吴怡 朱强 《中国食物与营养》 2024年第5期24-28,共5页
目的:特殊医学用途配方食品(FSMP)为全年龄段人群提供针对性营养支持,作为现代医疗保健体系中不可或缺的一部分,其弥补了传统饮食在特殊医疗需求前的不足,对整个医疗公共卫生领域产生了深远影响,针对FSMP产业发展现状不断完善其治理意... 目的:特殊医学用途配方食品(FSMP)为全年龄段人群提供针对性营养支持,作为现代医疗保健体系中不可或缺的一部分,其弥补了传统饮食在特殊医疗需求前的不足,对整个医疗公共卫生领域产生了深远影响,针对FSMP产业发展现状不断完善其治理意义重大。方法:通过文献分析,数据调研等方法,深入分析我国FSMP产业发展及治理现状。结果:尽管FSMP行业展现出强劲的增长势头,但仍面临标准体系不完善、监管衔接不畅和临床应用不充分的问题。结论:通过规范FSMP标准体系、畅通监管环节和提升临床应用认知度,可有效推动FSMP行业稳健发展,为实现“健康中国”提供持续稳定的可靠保障。 展开更多
关键词 特殊医学用途配方食品 产业态势 治理方式
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我国特种食品产业现状与发展趋势
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作者 梁莉 郝利民 +2 位作者 赵金山 张玉玉 孙宝国 《中国食品学报》 EI CAS CSCD 北大核心 2024年第5期58-74,共17页
特种食品能够满足特殊环境和特殊岗位作业人群的营养健康需求,同时可以快速响应各类突发紧急事件,其产业发展关系到安全应急、国家稳定和人民健康事业。近年来,我国特种食品产业发展势头强劲,创新能力不断增强,产业规模持续扩大,正逐步... 特种食品能够满足特殊环境和特殊岗位作业人群的营养健康需求,同时可以快速响应各类突发紧急事件,其产业发展关系到安全应急、国家稳定和人民健康事业。近年来,我国特种食品产业发展势头强劲,创新能力不断增强,产业规模持续扩大,正逐步成为新时期食品产业发展的新动向。本文回顾国内外特种食品的发展历程及产品特点,探讨其对保障特殊环境下人体营养健康的重要作用。通过文献调研系统梳理当前特种食品产业基础研究及技术发展现状。在此基础上,总结并展望特种食品产业面临的挑战以及未来发展趋势,旨在为我国特种食品研发及产业发展提供参考。 展开更多
关键词 特种食品 营养 功能 产业现状 未来趋势
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不同蛋白来源全营养特医食品乳剂的制备及其体外胃肠消化特性比较
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作者 郑键欣 杨韵仪 +3 位作者 陈文荣 万芝力 方素琼 杨晓泉 《现代食品科技》 CAS 北大核心 2024年第8期1-13,共13页
为了扩展不同类型蛋白质配料在液态全营养特医食品乳剂中的应用,探究动植物蛋白在特医乳液制备及消化特性方面的差异,该研究分别选取了酪蛋白(CS)、乳清蛋白(WPI)、大豆蛋白(SPI)、豌豆蛋白(PPI)和绿豆蛋白(MPI)作为界面稳定剂制备乳液... 为了扩展不同类型蛋白质配料在液态全营养特医食品乳剂中的应用,探究动植物蛋白在特医乳液制备及消化特性方面的差异,该研究分别选取了酪蛋白(CS)、乳清蛋白(WPI)、大豆蛋白(SPI)、豌豆蛋白(PPI)和绿豆蛋白(MPI)作为界面稳定剂制备乳液,系统探究灭菌工艺和营养成分添加对乳液性质的影响,并筛选其中热稳定的模板乳液(未添加维生素和矿物质)和特医乳液(添加维生素和矿物质)进行体外胃肠消化特性研究。CS、WPI、SPI和PPI制备的特医乳液状态良好,MPI特医乳液出现部分絮凝。灭菌后,WPI、MPI特医乳液分别发生凝胶和絮凝现象;CS、SPI和PPI特医乳液外观均匀,且制备的乳液具有较良好的稳定性,能在30 d内保持粒径和电位稳定。进一步选取CS、SPI和PPI制备的模板乳液和特医乳液进行体外消化性质分析,消化产物测试显示CS与SPI、PPI特医乳液的胃肠消化情况无明显差异,SPI和PPI的游离氨基释放量分别为21.095 mmol/L和19.524 mmol/L。该研究有助于理解不同类型蛋白配料在液态全营养特医乳剂中的应用表现,为液态高蛋白特医食品开发提供一定的技术支持。 展开更多
关键词 植物蛋白 豆球蛋白 特医食品 全营养乳液 胃肠消化性
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特殊人群保健食品食用适应性研究分析
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作者 李晓朋 董雨桐 +2 位作者 陈巧 武紫静 王玉伟 《食品工业科技》 CAS 北大核心 2024年第20期361-368,共8页
目的:儿童和孕期哺乳期妇女作为两类特殊人群,盲目摄入各类食品将会存在一定隐患,探究这两类人群在保健食品适宜性标识的占比差异情况,分析原因并从多角度为提高特殊人群食用保健食品安全性提出建议。方法:选取国家市场监督管理总局特... 目的:儿童和孕期哺乳期妇女作为两类特殊人群,盲目摄入各类食品将会存在一定隐患,探究这两类人群在保健食品适宜性标识的占比差异情况,分析原因并从多角度为提高特殊人群食用保健食品安全性提出建议。方法:选取国家市场监督管理总局特殊食品信息查询平台1996年6月~2022年12月功能声称注册数前十位的保健食品数据,从功能声称、获批时间及原料组成进行研究,利用SPSS Statistic 26软件对特殊人群在不适宜标识中的构成进行卡方检验和回归。结果:特殊人群在十类产品不适宜标识占比例均大于30%;特殊人群在同类产品的不适宜标识中的构成比存在显著差异(P<0.05);同类产品中不同适宜性标识所用高频原料具有显著差异(P<0.05)。结论:特殊的生理阶段和健康问题、产品原料的组成、逐步完善的监管制度是构成差异的主要因素。管理部门、生产销售企业和消费者三主体应形成合力,逐步加强顶层监管、完善内部规范、提高产品认知,从而促进保健食品的安全性不断提升。 展开更多
关键词 保健食品 特殊人群 适应性 卡方检验
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唾液酸对一种特殊医学用途全营养配方食品体外消化特性的影响
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作者 程园梦 丰珂珏 +5 位作者 左摇 胡雄 熊文 邓乾春 陈亚淑 李翔宇 《食品工业科技》 CAS 北大核心 2024年第18期42-48,共7页
本文探究了添加唾液酸(SA)对1~10岁全营养特医食品在体外模拟消化过程中脂肪及脂溶性营养素(如维生素E)消化特性的影响。结果表明,SA的添加促进了特医食品在水溶液中的分散,液滴尺寸从0.84±0.09μm下降到0.63±0.04μm,电位没... 本文探究了添加唾液酸(SA)对1~10岁全营养特医食品在体外模拟消化过程中脂肪及脂溶性营养素(如维生素E)消化特性的影响。结果表明,SA的添加促进了特医食品在水溶液中的分散,液滴尺寸从0.84±0.09μm下降到0.63±0.04μm,电位没有出现显著性变化(P>0.05)。SA的添加对特医食品中的脂肪酸在消化过程中的释放率与维生素E的生物可及性均有正向提高效果,脂肪酸释放率从76.65%提高到93.10%,维生素E生物可及率从79.75%提高到91.64%。此外,SA的加入对特医食品中脂肪消化过程中氧化稳定性无显著性影响(P>0.05)。本研究为后续SA应用于特医食品领域提供了参考依据。 展开更多
关键词 唾液酸 特医食品 体外消化 氧化稳定性
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可印刷绿色食品包装使用特种纸张综合性能研究
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作者 刘菁 陈卓 《造纸科学与技术》 2024年第7期118-121,126,共5页
食品所使用的包装纸除需要具备良好的物理、化学性能外,还需要具备一定的抗菌性能。为开发一种可印刷的绿色食品包装用特种纸张,以天然植物淀粉为基础材料,通过改性,添加增强纸张综合性能的纤维和添加剂等工艺,制备了一种食品包装用特... 食品所使用的包装纸除需要具备良好的物理、化学性能外,还需要具备一定的抗菌性能。为开发一种可印刷的绿色食品包装用特种纸张,以天然植物淀粉为基础材料,通过改性,添加增强纸张综合性能的纤维和添加剂等工艺,制备了一种食品包装用特种纸张。对该纸张的综合性能进行测试,结果表明:该纸张充分克服了一般淀粉食品包装纸韧性差、难印刷、长时间保存容易变脆等问题,是一种综合性能较为优异且可自然降解的绿色食品包装用特殊纸张。 展开更多
关键词 绿色食品包装 可印刷纸张 特种纸 综合性能
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利用新型红酵母苯丙氨酸解氨酶制备低苯丙氨酸酪蛋白
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作者 张阿娜 韩雪 +2 位作者 谷天一 辛凤姣 王钰璐 《生物技术通报》 CAS CSCD 北大核心 2024年第8期309-319,共11页
【目的】挖掘高活性、高稳定性的苯丙氨酸解氨酶(EC 4.3.1.24;penylalanine ammonia-lyase,PAL),为后续在制备无(低)苯丙氨酸特膳食品的应用奠定基础。【方法】从胶红酵母(Rhodotorula mucilaginosa)和双倒卵形红酵母(Rhodotorula diobo... 【目的】挖掘高活性、高稳定性的苯丙氨酸解氨酶(EC 4.3.1.24;penylalanine ammonia-lyase,PAL),为后续在制备无(低)苯丙氨酸特膳食品的应用奠定基础。【方法】从胶红酵母(Rhodotorula mucilaginosa)和双倒卵形红酵母(Rhodotorula diobovata)中克隆到基因RmPAL和RdPAL,并通过生物信息学分析两个酶的序列和结构特征;在大肠杆菌中异源表达纯化RmPAL和RdPAL蛋白,测定最适反应条件和底物特异性;通过高效液相色谱和苯丙氨酸试剂盒测定了RmPAL和RdPAL转化酸解酪蛋白(casein acid hydrolysate,CAH)中苯丙氨酸(L-phenylalanine,L-Phe)的能力。【结果】RmPAL和RdPAL是真菌来源的PAL,分别由3个结构域组成:MIO结构域(MIO domain)、核心结构域(core domain)和屏蔽结构域(shielding domain),活性中心具有催化氨基酸Tyr和底物特异性特征氨基酸His;RmPAL和RdPAL在溶液中均以四聚体形式存在,两个酶的最适pH和最适温度均为8.9和50℃,且具有较宽泛的pH和温度稳定性,优于黏红酵母来源的商用PAL酶;此外,两种酶均能催化L-Phe和酪氨酸(L-tyrosine,L-Tyr)反应,且对L-Phe的催化效率较高,约为L-Tyr的5倍,脱除酸解酪蛋白中L-Phe的转化率分别为88%和93%。【结论】RmPAL和RdPAL具有较强稳定性和L-Phe水解偏好性,可从食源蛋白中有效去除L-Phe。 展开更多
关键词 苯丙酮尿症 苯丙氨酸解氨酶 低L-Phe蛋白 催化活性 热稳定性 L-Phe转化率 特医食品
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