胃肠道调理专用奶粉对便秘小鼠肠道动力和菌群组成的影响

目的:研究胃肠道调理专用奶粉润肠通便作用及对肠道菌群的影响。方法:自制胃肠道调理专用奶粉后,以KM小鼠为实验对象,采用盐酸咯哌丁胺建立小鼠便秘模型,以小鼠一般状态、小肠推进率、排便情况、小肠组织观察为考察指标结合16S rRNA基因测序分析小鼠粪便,考察其对小鼠的润肠通便作用及肠道菌群的影响。结果:胃肠道调理专用奶粉各剂量能够显著(P<0.05)缩短小鼠首粒排便时间、显著(P<0.05)提高小鼠小肠推进率,但未对小鼠小肠杯状细胞结构造成破坏,且随着剂量的提升,奶粉的胃肠调理效果越好。胃肠道调理专用奶粉虽然没有明显改变肠道微生物的丰富度和均匀度,但是能够明显改变小鼠的肠道微生态结构组成,显著(P<0.05)增加某些益生菌Lactobacillus、Bifidobacterium的相对丰度。结论:胃肠道调理专用奶粉能够影响肠道运动,调节肠道菌群结构从而改善洛哌丁胺引起的便秘。

婴幼儿胃肠道食物消化研究进展

母乳和配方乳粉是婴幼儿的主要食物来源,婴幼儿胃肠道食物消化与其早期生长发育及长期健康状况密切相关.新生儿胃肠道发育不完善,常出现蛋白质过敏、乳糖不耐受和脂肪吸收不良等轻度消化问题,可通过饮食调节来改善.近年来,已有不同类型调理配方乳粉用于改善婴幼儿的胃肠道消化状况.本文介绍了婴幼儿胃肠道消化环境,阐述了婴幼儿对蛋白质、乳糖和脂肪等营养素的消化研究进展以及益生菌和益生元对婴幼儿肠道菌群的调节,重点介绍了婴幼儿对水解蛋白配方乳粉、无乳糖配方乳粉、1,3-二油酸-2-棕榈酸(oleic-palmitic-oleic,OPO)结构脂配方乳粉和益生元强化型配方乳粉的消化特性,对婴幼儿食品的未来研究进行了展望,提出了婴幼儿配方乳粉今后的3个发展方向.

Development of milk powder containing Lactobacillus plantarum NCIMB 8826 immobilized with prebiotic hi-maize starch and survival under simulated gastric and intestinal conditions

The objectives of this study were to develop a probiotic milk powder containing Lactobacillus plantarum NCIMB 8826 immobilized with prebiotic Hi-maize starch and to analyze cell viability after spray drying and exposure to simulated gastric and intestinal conditions.Milk powders containing free L.plantarum and cells immobilized with Hi-maize starch were assessed.Powders were evaluated during storage at 4°C for 15 days.After spray drying,at 0 and 15 days of storage both treatments had over 8 log CFU/g of viable cells and there were higher viable counts found for immobilized cells compared to free cells after 120 min in simulated gastric fluid.At 15 days of storage,immobilized cells had higher viable counts than free cells after exposure to simulated intestinal fluid for 120 min.The combined probiotic and prebiotic milk powder had stable viable cell counts at refrigerated storage conditions and under simulated gastric and intestinal transit.