Nerve growth factor（NGF） binds to TrkA and forms a NGF/TrkA complex at the cell surface,which is then internalized into signaling endosomes and promotes neuronal survival and neurite outgrowth.The small GTPase Rab5 ...Nerve growth factor（NGF） binds to TrkA and forms a NGF/TrkA complex at the cell surface,which is then internalized into signaling endosomes and promotes neuronal survival and neurite outgrowth.The small GTPase Rab5 is reported to localize on the plasma membrane and early endosomes,regulating endosome fusion.It was reported that endogenous Rab5 function may need to be suppressed during NGF-induced neurite outgrowth and cell differentiation.Two Rab5 homologs（MoRab5A：MGG_06241 and MoRab5B：MGG_01185） were characterized from the rice blast fungus Magnaporthe oryzae,and MoRab5 B was identified as the Rab5 ortholog promoting early endosomal fusion,while MoRab5 A specialized to perform a non-redundant function in endosomal sorting.In this study,we examined whether MoRab5 A and MoRab5 B play different roles in NGF-induced neurite outgrowth and cell differentiation in PC12 cells（a rat pheochromocytoma cell line）.Our data showed that MoRab5 B is a negative regulator of NGF signaling and neurite outgrowth in PC12 cells,similar to human Rab5（hRab5）.MoRab5B：WT inhibits NGF signaling-dependent neurite outgrowth while the dominant-negative MoRab5 B mutant（MoRab5B：DN） enhances NGF signaling and neurite outgrowth.In contrast,MoRab5A：WT and MoRab5A：DN both significantly promote NGF-induced neurite outgrowth,indicating that MoRab5 B is more similar to hRab5 than MoRab5 A in the regulation of NGF signal transduction.展开更多
The principle and structure of hydro-viscous drive(HVD) set is introduced. Through theoretical analysis of the HVD set start-up steadiness, the conclusions were obtained that the non-linearity of the pressure performa...The principle and structure of hydro-viscous drive(HVD) set is introduced. Through theoretical analysis of the HVD set start-up steadiness, the conclusions were obtained that the non-linearity of the pressure performance curve and dynamic pressure instability of the pilot-operated electro-hydraulic pressure relief valve, which is used for the HVD speed regulating purpose and usually works within micro flow-rate and low oil pressure state, are the main causes of the poor start-up steadiness. So a special speed regulating valve for HVD set is developed, with which the start-up steadiness of HVD set is much improved.展开更多
基金supported,in whole or in part,by the National Institutes of Health Grant,USA(R01GM074692)the National Nature Sciences Foundation of China(31328002and 31070124)a scholarship from the China Scholarship Council
文摘Nerve growth factor（NGF） binds to TrkA and forms a NGF/TrkA complex at the cell surface,which is then internalized into signaling endosomes and promotes neuronal survival and neurite outgrowth.The small GTPase Rab5 is reported to localize on the plasma membrane and early endosomes,regulating endosome fusion.It was reported that endogenous Rab5 function may need to be suppressed during NGF-induced neurite outgrowth and cell differentiation.Two Rab5 homologs（MoRab5A：MGG_06241 and MoRab5B：MGG_01185） were characterized from the rice blast fungus Magnaporthe oryzae,and MoRab5 B was identified as the Rab5 ortholog promoting early endosomal fusion,while MoRab5 A specialized to perform a non-redundant function in endosomal sorting.In this study,we examined whether MoRab5 A and MoRab5 B play different roles in NGF-induced neurite outgrowth and cell differentiation in PC12 cells（a rat pheochromocytoma cell line）.Our data showed that MoRab5 B is a negative regulator of NGF signaling and neurite outgrowth in PC12 cells,similar to human Rab5（hRab5）.MoRab5B：WT inhibits NGF signaling-dependent neurite outgrowth while the dominant-negative MoRab5 B mutant（MoRab5B：DN） enhances NGF signaling and neurite outgrowth.In contrast,MoRab5A：WT and MoRab5A：DN both significantly promote NGF-induced neurite outgrowth,indicating that MoRab5 B is more similar to hRab5 than MoRab5 A in the regulation of NGF signal transduction.
文摘The principle and structure of hydro-viscous drive(HVD) set is introduced. Through theoretical analysis of the HVD set start-up steadiness, the conclusions were obtained that the non-linearity of the pressure performance curve and dynamic pressure instability of the pilot-operated electro-hydraulic pressure relief valve, which is used for the HVD speed regulating purpose and usually works within micro flow-rate and low oil pressure state, are the main causes of the poor start-up steadiness. So a special speed regulating valve for HVD set is developed, with which the start-up steadiness of HVD set is much improved.
