Quality control and screening of active substances in traditional Chinese medicines have been performed using fingerprint analysis. The spectrum-effect relationship between chromatography fingerprints and efficacy of ...Quality control and screening of active substances in traditional Chinese medicines have been performed using fingerprint analysis. The spectrum-effect relationship between chromatography fingerprints and efficacy of herbal drugs is considered as a potentially useful method for determining active ingredients in complex mixtures. The study was designed to develop a method for determining the bioactive components of a compound Chinese medicine called Tiefeng based on spectrum-effect relationships between high-performance liquid chromatography （HPLC） fingerprints and anticoccidial activities. Four peaks of the established HPLC fingerprint indicate the main bioactive components of this medicine. In addition, pharrnacodynamic atlas was defined and used to assess the anticoccidial activity of Tiefeng from different sources for the first time. We found that the level of anticoccidial activity of Tiefeng was consistent with the degree of similarity between the pharmacodynamic atlas and chromatogram of any sample. Furthermore, effect of this medicine was related with the main active constituents, along with the origin and the harvesting time.展开更多
The traditional Chinese medicine of Radix Hedysari plays an important role in invigorating gas for ascending, benefiting blood for promoting production of fluid, and promoting circulation for removing obstruction in c...The traditional Chinese medicine of Radix Hedysari plays an important role in invigorating gas for ascending, benefiting blood for promoting production of fluid, and promoting circulation for removing obstruction in collaterals, which is consistent with the principle of treatment for osteoporosis. This study is designed to investigate the bioactive components on increasing peak bone mass (PBM) by exploring the spectrum-effect relationship between chromatography fingerprints and effect. Multiple indicators are selected to evaluate the pharmacological activity. In fingerprints, 21 common peaks are obtained, five of which are identified. Furthermore, gray relational analysis (GRA) is a quantitative method of gray system theory and is used to describe the correlation degree of common peaks and pharmacological activities with relational value. 21 components are then divided into three different regions, of which ononin and calycosin play an extremely significant role in increasing PBM. In addition, factor analysis and hierarchical cluster analysis (HCA) are used to screen the optimal producing area for Radix Hedysari. This provides a comprehensive and efficient method to improve the quality evaluation of Radix Hedysari, confirming the bioactive components for PBM-enhancement and further develop its medicinal value.展开更多
为探讨菊茎叶总黄酮(total flavonoids from stems and leaves of Chrysanthemum morifolium,TFCSL)抗氧化应激的活性成分,阐明其药效物质基础和作用机制。采用HPLC建立不同批次TFCSL指纹图谱;以高浓度葡萄糖诱导人脐静脉内皮细胞建立...为探讨菊茎叶总黄酮(total flavonoids from stems and leaves of Chrysanthemum morifolium,TFCSL)抗氧化应激的活性成分,阐明其药效物质基础和作用机制。采用HPLC建立不同批次TFCSL指纹图谱;以高浓度葡萄糖诱导人脐静脉内皮细胞建立氧化损伤模型,将丙二醛含量、乳酸脱氢酶含量和超氧化物歧化酶活性作为药效指标;采用灰色关联度和偏最小二乘法分析其谱-效关系确定抗氧化药效物质基础;基于网络药理学结合分子对接探究核心靶点及作用通路。从12批次TFCSL指纹图谱中确定12个共有峰,指认其中9个化学成分;各批次总黄酮样品均可减少细胞凋亡、降低丙二醛及乳酸脱氢酶含量、提高超氧化物歧化酶活性;综合2种数学模型确定峰5(芹菜素-7-O-β-D-葡萄糖苷)、峰6(异绿原酸C)、峰7(香叶木素-7-O-β-D-葡萄糖苷)为抗氧化物质基础;筛选出的3个活性成分作用于抗氧化应激的33个靶点;关键靶点为TNF、CASP3、EDNRA、XDH、PTGS2、MMP2,主要涉及脂质和动脉粥样硬化信号通路、IL-17信号通路、糖尿病并发症AGE-RAGE信号通路、TNF通路、MPKA通路等信号通路;分子对接结果显示活性成分与关键靶点之间均有较好的结合力。表明TFCSL抗氧化应激的物质基础可能为芹菜素-7-O-β-D-葡萄糖苷、异绿原酸C、香叶木素-7-O-β-D-葡萄糖苷,推测通过TNF、CASP3等靶点作用动脉粥样硬化信号通路、IL17信号通路发挥作用,体现菊茎叶多成分、多靶点抗氧化的作用特点。展开更多
基金funded by the National Key Technology R&D Program, China (2008 BADB4B05-02)
文摘Quality control and screening of active substances in traditional Chinese medicines have been performed using fingerprint analysis. The spectrum-effect relationship between chromatography fingerprints and efficacy of herbal drugs is considered as a potentially useful method for determining active ingredients in complex mixtures. The study was designed to develop a method for determining the bioactive components of a compound Chinese medicine called Tiefeng based on spectrum-effect relationships between high-performance liquid chromatography （HPLC） fingerprints and anticoccidial activities. Four peaks of the established HPLC fingerprint indicate the main bioactive components of this medicine. In addition, pharrnacodynamic atlas was defined and used to assess the anticoccidial activity of Tiefeng from different sources for the first time. We found that the level of anticoccidial activity of Tiefeng was consistent with the degree of similarity between the pharmacodynamic atlas and chromatogram of any sample. Furthermore, effect of this medicine was related with the main active constituents, along with the origin and the harvesting time.
基金supported by the National Natural Science Funds of China(Grant No.81703664)Science and Technology Funds of Lanzhou,China(Grant No.201603111)
文摘The traditional Chinese medicine of Radix Hedysari plays an important role in invigorating gas for ascending, benefiting blood for promoting production of fluid, and promoting circulation for removing obstruction in collaterals, which is consistent with the principle of treatment for osteoporosis. This study is designed to investigate the bioactive components on increasing peak bone mass (PBM) by exploring the spectrum-effect relationship between chromatography fingerprints and effect. Multiple indicators are selected to evaluate the pharmacological activity. In fingerprints, 21 common peaks are obtained, five of which are identified. Furthermore, gray relational analysis (GRA) is a quantitative method of gray system theory and is used to describe the correlation degree of common peaks and pharmacological activities with relational value. 21 components are then divided into three different regions, of which ononin and calycosin play an extremely significant role in increasing PBM. In addition, factor analysis and hierarchical cluster analysis (HCA) are used to screen the optimal producing area for Radix Hedysari. This provides a comprehensive and efficient method to improve the quality evaluation of Radix Hedysari, confirming the bioactive components for PBM-enhancement and further develop its medicinal value.
文摘为探讨菊茎叶总黄酮(total flavonoids from stems and leaves of Chrysanthemum morifolium,TFCSL)抗氧化应激的活性成分,阐明其药效物质基础和作用机制。采用HPLC建立不同批次TFCSL指纹图谱;以高浓度葡萄糖诱导人脐静脉内皮细胞建立氧化损伤模型,将丙二醛含量、乳酸脱氢酶含量和超氧化物歧化酶活性作为药效指标;采用灰色关联度和偏最小二乘法分析其谱-效关系确定抗氧化药效物质基础;基于网络药理学结合分子对接探究核心靶点及作用通路。从12批次TFCSL指纹图谱中确定12个共有峰,指认其中9个化学成分;各批次总黄酮样品均可减少细胞凋亡、降低丙二醛及乳酸脱氢酶含量、提高超氧化物歧化酶活性;综合2种数学模型确定峰5(芹菜素-7-O-β-D-葡萄糖苷)、峰6(异绿原酸C)、峰7(香叶木素-7-O-β-D-葡萄糖苷)为抗氧化物质基础;筛选出的3个活性成分作用于抗氧化应激的33个靶点;关键靶点为TNF、CASP3、EDNRA、XDH、PTGS2、MMP2,主要涉及脂质和动脉粥样硬化信号通路、IL-17信号通路、糖尿病并发症AGE-RAGE信号通路、TNF通路、MPKA通路等信号通路;分子对接结果显示活性成分与关键靶点之间均有较好的结合力。表明TFCSL抗氧化应激的物质基础可能为芹菜素-7-O-β-D-葡萄糖苷、异绿原酸C、香叶木素-7-O-β-D-葡萄糖苷,推测通过TNF、CASP3等靶点作用动脉粥样硬化信号通路、IL17信号通路发挥作用,体现菊茎叶多成分、多靶点抗氧化的作用特点。
