Controlled release of the functional factors is the key to improve clinical therapeutic efficacy during the tissue repair and regeneration. The thrce-dimensional （3D） scaffold can provide not only physical propertie...Controlled release of the functional factors is the key to improve clinical therapeutic efficacy during the tissue repair and regeneration. The thrce-dimensional （3D） scaffold can provide not only physical properties such as high strength and porosity hut also an optimal environment to enhance tissue regeneration. Sphingosine 1-phosphate （SIP）, an angiogenlc factor, was loaded into mesoporous silica nanoparticles （MSNs） and then incorporated into poly （ L-lactic add ） （ PLLA ） nanofibrons scaffold, which was fabricated by thermally induced phase separation （TIPS） method. The prepared scaffolds were examined by attenuated total reflection Fourier transform infrared spectroscopy （ATR-FTIR）, scanning electron microscopy （ SEM）, and transmission electron microscopy （TEM） and compressive mechanical test. The ATR-FTIR result demonstrated the existence of MSNs in the PLLA nanofibrous scaffold. The SEM images showed that PLLA scaffold had regular pore channel, interconnected pores and nanofibrous structure. The addition of MSNs at appropriate content had no visible effect on the structure of scaffold. The compressive modulus of scaffold containing MSNs was higher than that of the scaffold without MSNs. Furthermore, fluorescein isothiocyanate （FTTC） was used as model molecule to investigate the release behavior of SIP from MSNs- incorporated PLLA （MSNs/PLLA） nanofibrons scaffold. The result showed that the composite scaffold largely reduced the initial burst release and exhibited prolonged release of FITC than MSNs. Thus, these results indicated that SIP-loaded composite uanofibrons scaffold has potential applications for bone tissue engneering.展开更多
目的:探讨开玄解毒方对于咪喹莫特诱导银屑病样小鼠模型的症状、体征改善及其对1-磷酸鞘氨醇(sphingosine-1-phosphate,S1P)水平的调节作用。方法:选用BALB/c小鼠25只,随机均分为正常组、模型组、甲氨蝶呤组、开玄解毒中剂量组和高剂量...目的:探讨开玄解毒方对于咪喹莫特诱导银屑病样小鼠模型的症状、体征改善及其对1-磷酸鞘氨醇(sphingosine-1-phosphate,S1P)水平的调节作用。方法:选用BALB/c小鼠25只,随机均分为正常组、模型组、甲氨蝶呤组、开玄解毒中剂量组和高剂量组,除正常组外,其余组采用外涂咪喹莫特法建立银屑病样皮损模型,连续干预7 d后取材。期间对皮损拍照并计算银屑病面积和严重程度指数(psoriasis area and severity index,PASI)评分;HE染色观察皮损病理改变及测量表皮厚度;对脾脏及胸腺拍照并称重;免疫组化法检测表皮Ki67、S1P表达;蛋白质印迹法检测皮损KRT17、Claudin、Occludin表达;qRT-PCR检测IL-17、IL-23、S1PRs mRNA表达水平。结果:与模型组比较,开玄解毒方可缓解咪喹莫特诱导的小鼠银屑病样皮损,降低PASI评分,减轻表皮增生程度(P均<0.01),改善胸腺、脾脏指数及血管增生情况(P<0.01或P<0.05),降低炎症因子IL-17、IL-23 mRNA表达(P<0.01),修复皮肤屏障(P<0.05)。同时,开玄解毒方可下调表皮中S1P及S1PRs mRNA表达(P<0.05)。结论:开玄解毒方能够减轻银屑病局部皮损症状,其药效机制可能与调节S1P水平有关。展开更多
基金National Natural Science Foundations of China(Nos.31271028,31570984)International Cooperation Fund of the Science and Technology Commission of Shanghai Municipality,China(No.15540723400)+2 种基金Open Foundation of State Key Laboratory for Modification of Chemical Fibers,Polymer Materials,China(No.LK1416)the Innovation Funds of Donghua University,China(No.15D310516)“111 Project” Biomedical Textile Materials Science and Technology,China(No.B07024)
文摘Controlled release of the functional factors is the key to improve clinical therapeutic efficacy during the tissue repair and regeneration. The thrce-dimensional （3D） scaffold can provide not only physical properties such as high strength and porosity hut also an optimal environment to enhance tissue regeneration. Sphingosine 1-phosphate （SIP）, an angiogenlc factor, was loaded into mesoporous silica nanoparticles （MSNs） and then incorporated into poly （ L-lactic add ） （ PLLA ） nanofibrons scaffold, which was fabricated by thermally induced phase separation （TIPS） method. The prepared scaffolds were examined by attenuated total reflection Fourier transform infrared spectroscopy （ATR-FTIR）, scanning electron microscopy （ SEM）, and transmission electron microscopy （TEM） and compressive mechanical test. The ATR-FTIR result demonstrated the existence of MSNs in the PLLA nanofibrous scaffold. The SEM images showed that PLLA scaffold had regular pore channel, interconnected pores and nanofibrous structure. The addition of MSNs at appropriate content had no visible effect on the structure of scaffold. The compressive modulus of scaffold containing MSNs was higher than that of the scaffold without MSNs. Furthermore, fluorescein isothiocyanate （FTTC） was used as model molecule to investigate the release behavior of SIP from MSNs- incorporated PLLA （MSNs/PLLA） nanofibrons scaffold. The result showed that the composite scaffold largely reduced the initial burst release and exhibited prolonged release of FITC than MSNs. Thus, these results indicated that SIP-loaded composite uanofibrons scaffold has potential applications for bone tissue engneering.
文摘目的:探讨开玄解毒方对于咪喹莫特诱导银屑病样小鼠模型的症状、体征改善及其对1-磷酸鞘氨醇(sphingosine-1-phosphate,S1P)水平的调节作用。方法:选用BALB/c小鼠25只,随机均分为正常组、模型组、甲氨蝶呤组、开玄解毒中剂量组和高剂量组,除正常组外,其余组采用外涂咪喹莫特法建立银屑病样皮损模型,连续干预7 d后取材。期间对皮损拍照并计算银屑病面积和严重程度指数(psoriasis area and severity index,PASI)评分;HE染色观察皮损病理改变及测量表皮厚度;对脾脏及胸腺拍照并称重;免疫组化法检测表皮Ki67、S1P表达;蛋白质印迹法检测皮损KRT17、Claudin、Occludin表达;qRT-PCR检测IL-17、IL-23、S1PRs mRNA表达水平。结果:与模型组比较,开玄解毒方可缓解咪喹莫特诱导的小鼠银屑病样皮损,降低PASI评分,减轻表皮增生程度(P均<0.01),改善胸腺、脾脏指数及血管增生情况(P<0.01或P<0.05),降低炎症因子IL-17、IL-23 mRNA表达(P<0.01),修复皮肤屏障(P<0.05)。同时,开玄解毒方可下调表皮中S1P及S1PRs mRNA表达(P<0.05)。结论:开玄解毒方能够减轻银屑病局部皮损症状,其药效机制可能与调节S1P水平有关。
