[Objectives]This study aimed to establish HPLC chromatograms of decoction pieces,standard decoction and formula granules of Spica Prunellae.[Methods]The chromatographic conditions were as follows:column,SHISEIDO CAPCE...[Objectives]This study aimed to establish HPLC chromatograms of decoction pieces,standard decoction and formula granules of Spica Prunellae.[Methods]The chromatographic conditions were as follows:column,SHISEIDO CAPCELL PAK C18 MGII column(4.6 mm×250 mm,5μm);mobile phase,acetonitrile-0.1%phosphoric acid;gradient elution;detection wavelength,280 nm;flow rate,1.0 mL/min;and column temperature,30℃.The correlation between the decoction pieces,standard decoction and formula granules of Spica Prunellae was analyzed by specific chromatograms.[Results]The fingerprint chromatograms of decoction pieces,standard decoction and formula granules of Spica Prunellae showed five common peaks,with good correlation.Among the five common peaks,four of them were tanshinol,protocatechuic acid,caffeic acid and rosmarinic acid.[Conclusions]The main chemical constituents of decoction pieces,standard decoction and formula granules of Spica Prunellae are basically the same.The HPLC specific chromatogram established can be used for the quality control of Spica Prunellae formula granules.展开更多
[Objectives] The study aimed to optimize the extraction process of Prunellae Spica compound to provide an experimental basis for the development and utilization of the compound. [Methods]Through orthogonal test,the op...[Objectives] The study aimed to optimize the extraction process of Prunellae Spica compound to provide an experimental basis for the development and utilization of the compound. [Methods]Through orthogonal test,the optimal extraction process of Prunellae Spica compound was chosen by using the yield of dry paste and ursolic acid content as evaluation indicators. [Results]The optimal extraction process of Prunellae Spica compound is as follows: solvent dosage was 16 times,and extraction frequency was 3 times,while extraction time was 1 h.[Conclusions]The extraction process of Prunellae Spica compound was stable and feasible,and this study can provide a theoretical basis for further preparation of the compound.展开更多
As important drugs for the treatment of nodular goiter(NG),Prunella Spica preparations are widely used clinically,and have a significant effect on NG.Various active ingredients in the preparations intervene in the for...As important drugs for the treatment of nodular goiter(NG),Prunella Spica preparations are widely used clinically,and have a significant effect on NG.Various active ingredients in the preparations intervene in the formation of NG by inhibiting the proliferation of thyroid follicular cells,promoting cell apoptosis,regulating immunity,improving the microcirculation of thyroid tissue and other mechanisms,and can reduce the postoperative recurrence of NG.展开更多
目的筛选夏枯草的质量标志物,并建立其含量测定的方法。方法利用UPLC Q Exactive Plus Orbitrap高分辨液质联用仪进行全成分分析,采用高效液相色谱(HPLC)法建立夏枯草的指纹图谱,对20批次夏枯草药材进行化学计量学分析,筛选夏枯草的质...目的筛选夏枯草的质量标志物,并建立其含量测定的方法。方法利用UPLC Q Exactive Plus Orbitrap高分辨液质联用仪进行全成分分析,采用高效液相色谱(HPLC)法建立夏枯草的指纹图谱,对20批次夏枯草药材进行化学计量学分析,筛选夏枯草的质量标志物并测定含量。综合分析相关的文献数据,研究不同产地夏枯草的迷迭香酸含量变化,进而评价药材质量。结果从夏枯草中共鉴定出72个化合物,其中有机酸类化合物36个,占总数的50%;20批次夏枯草的指纹图谱中共标记了8个共有峰,指认出咖啡酸、芦丁、金丝桃苷和迷迭香酸4个成分,各批次之间的相似度大于0.900。化学计量学分析结果表明,迷迭香酸可能是夏枯草的潜在质量标志物。测定20批次夏枯草中迷迭香酸含量,发现有5个批次不合格。综合文献分析了158批次夏枯草,发现迷迭香酸含量的整体合格率为59.77%。结论迷迭香酸是夏枯草的潜在质量标志物,其含量能有效地评价不同产地夏枯草药材的质量。展开更多
基金Supported by Scientific Research and Technology Development Project of Nanning City(20173158-5).
文摘[Objectives]This study aimed to establish HPLC chromatograms of decoction pieces,standard decoction and formula granules of Spica Prunellae.[Methods]The chromatographic conditions were as follows:column,SHISEIDO CAPCELL PAK C18 MGII column(4.6 mm×250 mm,5μm);mobile phase,acetonitrile-0.1%phosphoric acid;gradient elution;detection wavelength,280 nm;flow rate,1.0 mL/min;and column temperature,30℃.The correlation between the decoction pieces,standard decoction and formula granules of Spica Prunellae was analyzed by specific chromatograms.[Results]The fingerprint chromatograms of decoction pieces,standard decoction and formula granules of Spica Prunellae showed five common peaks,with good correlation.Among the five common peaks,four of them were tanshinol,protocatechuic acid,caffeic acid and rosmarinic acid.[Conclusions]The main chemical constituents of decoction pieces,standard decoction and formula granules of Spica Prunellae are basically the same.The HPLC specific chromatogram established can be used for the quality control of Spica Prunellae formula granules.
基金Supported by Talent Training and Construction Project of Professor Yang Shilin’s Team in 2017(YSL17010)
文摘[Objectives] The study aimed to optimize the extraction process of Prunellae Spica compound to provide an experimental basis for the development and utilization of the compound. [Methods]Through orthogonal test,the optimal extraction process of Prunellae Spica compound was chosen by using the yield of dry paste and ursolic acid content as evaluation indicators. [Results]The optimal extraction process of Prunellae Spica compound is as follows: solvent dosage was 16 times,and extraction frequency was 3 times,while extraction time was 1 h.[Conclusions]The extraction process of Prunellae Spica compound was stable and feasible,and this study can provide a theoretical basis for further preparation of the compound.
基金Supported by the Key Project of Teaching Reform Research of Taishan Vocational College of Nursing(202307).
文摘As important drugs for the treatment of nodular goiter(NG),Prunella Spica preparations are widely used clinically,and have a significant effect on NG.Various active ingredients in the preparations intervene in the formation of NG by inhibiting the proliferation of thyroid follicular cells,promoting cell apoptosis,regulating immunity,improving the microcirculation of thyroid tissue and other mechanisms,and can reduce the postoperative recurrence of NG.
文摘目的筛选夏枯草的质量标志物,并建立其含量测定的方法。方法利用UPLC Q Exactive Plus Orbitrap高分辨液质联用仪进行全成分分析,采用高效液相色谱(HPLC)法建立夏枯草的指纹图谱,对20批次夏枯草药材进行化学计量学分析,筛选夏枯草的质量标志物并测定含量。综合分析相关的文献数据,研究不同产地夏枯草的迷迭香酸含量变化,进而评价药材质量。结果从夏枯草中共鉴定出72个化合物,其中有机酸类化合物36个,占总数的50%;20批次夏枯草的指纹图谱中共标记了8个共有峰,指认出咖啡酸、芦丁、金丝桃苷和迷迭香酸4个成分,各批次之间的相似度大于0.900。化学计量学分析结果表明,迷迭香酸可能是夏枯草的潜在质量标志物。测定20批次夏枯草中迷迭香酸含量,发现有5个批次不合格。综合文献分析了158批次夏枯草,发现迷迭香酸含量的整体合格率为59.77%。结论迷迭香酸是夏枯草的潜在质量标志物,其含量能有效地评价不同产地夏枯草药材的质量。
