Some haplotypes of the sucrose synthase gene TaSus1 are associated with thousand-grain weight(TGW)in wheat(Triticum aestivum L.).However,no mutations have been identified within the gene to test this association.The e...Some haplotypes of the sucrose synthase gene TaSus1 are associated with thousand-grain weight(TGW)in wheat(Triticum aestivum L.).However,no mutations have been identified within the gene to test this association.The effects of TaSus1 on grain number per spike(GNS)also are largely unknown.Our previous genome-wide association study identified TaSus-A1 as a candidate gene controlling fertile spikelet number per spike(FSN).In the present study,we generated two independent mutants for the three TaSus1 homoeologs by CRISPR/Cas9-mediated genome editing.The triple mutants displayed lower FSN,GNS,grain number per spikelet(GNST),and TGW than wild-type plants.In 306 hexaploid wheat accessions,two single-nucleotide polymorphisms in TaSus-A1 contributed differently to GNS.Introgression of the two alleles into a wheat genetic background confirmed their effects.The alleles differed in geographical distribution among the accessions.展开更多
Grain size is one of the determinants of grain yield,and identifying the genetic loci that control grain size will be helpful for increasing grain yield.In our previous study,a quantitative trait locus(QTL)for grain l...Grain size is one of the determinants of grain yield,and identifying the genetic loci that control grain size will be helpful for increasing grain yield.In our previous study,a quantitative trait locus(QTL)for grain length(GL),QGl.cau-2D.1,was identified from an F2 population developed from the cross between the natural(TAA10)and synthetic(XX329)allohexaploid wheat.In the present study,we mainly fine mapped and validated its genetic effects.To this end,multiple near-isogenic lines(NILs)were obtained through marker-assisted selection with TAA10 as the recurrent parent.The secondary populations derived from 25 heterozygous recombinants were used for fine mapping of QGl.cau-2D.1,and the allele from XX329 significantly increased GL,thousand-grain weight(TGW),total spikelet number per spike(TSN)and spike compactness(SC).Using NILs for XX329(2D+)and TAA10(2D−),we determined the genetic and pleiotropic effects of QGl.cau-2D.1.The target sequences were aligned with the wheat reference genome RefSeq v2.1 and spanned an~0.9 Mb genomic region.TraesCS2D03G0114900(ortholog of Os03g0594700)was predicted as the candidate gene based on whole-genome re-sequencing and expression analyses.In summary,the map-based cloning of QGl.cau-2D.1 will be useful for improving grain weight with enhanced GL and TSN.展开更多
qSsnps-5D为一个控制不育小穗数的主效稳定QTL,其优异等位基因来自小麦骨干亲本京411。本研究利用科农9204×京411衍生的包含187个家系的重组自交系群体(KJ-RIL,recombinant inbred lines derived from the cross of Kenong 9204 an...qSsnps-5D为一个控制不育小穗数的主效稳定QTL,其优异等位基因来自小麦骨干亲本京411。本研究利用科农9204×京411衍生的包含187个家系的重组自交系群体(KJ-RIL,recombinant inbred lines derived from the cross of Kenong 9204 and Jing 411)及314份育成品种(系)组成的自然群体对其进行遗传及育种选择效应解析,明确其对产量性状的遗传效应,分析其在育种过程中的选择应用情况,评价其未来育种应用潜力。试验结果表明,qSsnps-5D在8套数据集中被定位于5D染色体上0.72~4.13 Mb之间,跨度约3.41 Mb。基于KJ-RIL群体及自然群体分析结果均表明,来自京411的优异等位基因可增加单株穗数,但对千粒重表现为极显著负向效应;其对穗粒数、单株产量的影响在两套群体的分析结果不一致。在qSsnps-5D靶区间内选择2个紧密连锁的SNP标记AX-110565536和AX-86170796对314份自然群体进行目标QTL单倍型分析;结果显示,国外品种对qSsnps-5D优异单倍型(Hap-GG-CC)的选择利用率最高;中国品种中青海省、四川省和河南省3个省份优异单倍型品种占比较高,而山东、北京、陕西和河北4地对qSsnps-5D优异单倍型选择利用率较低。时间跨度显示,qSsnps-5D优异单倍型Hap-GG-CC选择利用效率随时间推移在我国呈下降趋势。为便于qSsnps-5D后期分子育种应用,本研究开发了一个基于PCR检测技术的InDel分子标记,命名为5D-1620921,其带型扩增清晰,可重复性好,为qSsnps-5D分子育种应用提供理论支撑。展开更多
基金This work was supported by the Strategic Priority Research Program of Chinese Academy of Sciences(XDA24010104-2).
文摘Some haplotypes of the sucrose synthase gene TaSus1 are associated with thousand-grain weight(TGW)in wheat(Triticum aestivum L.).However,no mutations have been identified within the gene to test this association.The effects of TaSus1 on grain number per spike(GNS)also are largely unknown.Our previous genome-wide association study identified TaSus-A1 as a candidate gene controlling fertile spikelet number per spike(FSN).In the present study,we generated two independent mutants for the three TaSus1 homoeologs by CRISPR/Cas9-mediated genome editing.The triple mutants displayed lower FSN,GNS,grain number per spikelet(GNST),and TGW than wild-type plants.In 306 hexaploid wheat accessions,two single-nucleotide polymorphisms in TaSus-A1 contributed differently to GNS.Introgression of the two alleles into a wheat genetic background confirmed their effects.The alleles differed in geographical distribution among the accessions.
基金supported by the National Key Research and Development Program of China(32172069).
文摘Grain size is one of the determinants of grain yield,and identifying the genetic loci that control grain size will be helpful for increasing grain yield.In our previous study,a quantitative trait locus(QTL)for grain length(GL),QGl.cau-2D.1,was identified from an F2 population developed from the cross between the natural(TAA10)and synthetic(XX329)allohexaploid wheat.In the present study,we mainly fine mapped and validated its genetic effects.To this end,multiple near-isogenic lines(NILs)were obtained through marker-assisted selection with TAA10 as the recurrent parent.The secondary populations derived from 25 heterozygous recombinants were used for fine mapping of QGl.cau-2D.1,and the allele from XX329 significantly increased GL,thousand-grain weight(TGW),total spikelet number per spike(TSN)and spike compactness(SC).Using NILs for XX329(2D+)and TAA10(2D−),we determined the genetic and pleiotropic effects of QGl.cau-2D.1.The target sequences were aligned with the wheat reference genome RefSeq v2.1 and spanned an~0.9 Mb genomic region.TraesCS2D03G0114900(ortholog of Os03g0594700)was predicted as the candidate gene based on whole-genome re-sequencing and expression analyses.In summary,the map-based cloning of QGl.cau-2D.1 will be useful for improving grain weight with enhanced GL and TSN.
文摘qSsnps-5D为一个控制不育小穗数的主效稳定QTL,其优异等位基因来自小麦骨干亲本京411。本研究利用科农9204×京411衍生的包含187个家系的重组自交系群体(KJ-RIL,recombinant inbred lines derived from the cross of Kenong 9204 and Jing 411)及314份育成品种(系)组成的自然群体对其进行遗传及育种选择效应解析,明确其对产量性状的遗传效应,分析其在育种过程中的选择应用情况,评价其未来育种应用潜力。试验结果表明,qSsnps-5D在8套数据集中被定位于5D染色体上0.72~4.13 Mb之间,跨度约3.41 Mb。基于KJ-RIL群体及自然群体分析结果均表明,来自京411的优异等位基因可增加单株穗数,但对千粒重表现为极显著负向效应;其对穗粒数、单株产量的影响在两套群体的分析结果不一致。在qSsnps-5D靶区间内选择2个紧密连锁的SNP标记AX-110565536和AX-86170796对314份自然群体进行目标QTL单倍型分析;结果显示,国外品种对qSsnps-5D优异单倍型(Hap-GG-CC)的选择利用率最高;中国品种中青海省、四川省和河南省3个省份优异单倍型品种占比较高,而山东、北京、陕西和河北4地对qSsnps-5D优异单倍型选择利用率较低。时间跨度显示,qSsnps-5D优异单倍型Hap-GG-CC选择利用效率随时间推移在我国呈下降趋势。为便于qSsnps-5D后期分子育种应用,本研究开发了一个基于PCR检测技术的InDel分子标记,命名为5D-1620921,其带型扩增清晰,可重复性好,为qSsnps-5D分子育种应用提供理论支撑。
