Association Analysis and Identification of SNP Markers for Stemphylium Leaf Spot (Stemphylium botryosum f. sp. spinacia) Resistance in Spinach (Spinacia oleracea)

Stemphylium leaf spot, caused by Stemphylium botryosum f. sp. spinacia, is an important fungal disease of spinach (Spinacia oleracea L.). The aim of this study was to conduct association analysis to identify single nucleotide polymorphism (SNP) markers associated with Stemphylium leaf spot resistance in spinach. A total of 273 spinach genotypes, including 265 accessions from the USDA spinach germplasm collection and eight commercial cultivars, were used in this study. Phenotyping for Stemphylium leaf spot resistance was evaluated in greenhouse;genotyping was conducted using genotyping by sequencing (GBS) with 787 SNPs;and single marker regression, general linear model, and mixed linear model were used for association analysis of Stemphylium leaf spot. Spinach genotypes showed a skewed distribution for Stemphylium leaf spot resistance, with a range from 0.2% to 23.5% disease severity, suggesting that Stemphylium leaf spot resistance in spinach is a complex, quantitative trait. Association analysis indicated that eight SNP markers, AYZV02052595_115, AYZV02052595_122, AYZV02057770_10404, AYZV02129827_205, AYZV0-2152692_182, AYZV02180153_337, AYZV02225889_197, and AYZV02258563_213 were strongly associated with Stemphylium leaf spot resistance, with a Log of the Odds (LOD) of 2.5 or above. The SNP markers may provide a tool to select for Stemphylium leaf spot resistance in spinach breeding programs through marker-assisted selection (MAS).

Optimization of micropropagation and <i>Agrobacterium</i>-mediated gene transformation to spinach (<i>Spinacia oleracea</i>L.)

Spinach is one of the dioecious plant which is considered as a model plant in genetic and molecular studies of sex determination because of its special characteristics such as low chromosome number and short life cycle. An efficient protocol for Spinacia oleracea Agrobacterium-mediated gene transformation was developed. The leaf disks, roots, hypocotyls and cotyledons of this plant were inoculated with LBA4404. LBA4404 carrying pCAMBIA3301 binary vector with 35SCaMV gusint and 35SCaMV bar cassettes. Effects of two preparation condition (induction of vir genes and noninduction) were considered. Also effects of different number days of co-cultivation and pre-culture of explants were examined. After co-cultivation, the explants were transferred to regeneration medium containing 250 mg·L-1 Carbeniciline. Transient expression efficiency was calculated based on the number of blue spots per explants one week after inoculation. Based on the results of transient expression, stable transformation was carried out. After formation of callus the histochemical GUS assay was carried out on some parts of them and other parts were leaved for being regenerated.

Physiological Response of Halophyte (<i>Suaeda altissima</i>(L.) Pall.) and Glycophyte (<i>Spinacia oleracea</i>L.) to Salinity

We have done a comparative study of ion status, growth and biochemical parameters in shoots and roots of seablite (Suaeda altissima (L.) Pall.) and spinach (Spinacia oleracea L.) grown with different salinity levels in the medium (0.5 - 750 mМ). A distinctive feature of the halophyte was a high Na+ content in tissues at its low concentration in the medium (0.5 mM). In these conditions, Na+ accumulation in seablite roots was four-fold higher than in spinach roots, and Na+ content in seablite leaves was almost 20-fold higher than in spinach. Together with an increase in sodium concentration in the medium, K+ content decreased six-fold in seablite leaves, while in spinach it did not decrease so drastically. We can suppose that in the halophyte, some processes occur only in the presence of sodium, and these functions of sodium cannot be fully fulfilled by potassium. Analysis of protein and total nitrogen content in tissues shows that at high salinity, the ability to synthesize non-protein nitrogen-containing compounds increases in the halophyte and decreases in the glycophyte. Data on proline content dynamics show that its increase in tissues of spinach (salinity levels 150 and 250 mМ) and seablite (salinity levels 0.5 and 750 mМ) is an indicator of plant injury. In seablite and spinach, proline is not a major osmoregulator. Its concentration both in roots and leaves was no more than 2.5 μmol/g fresh weight. The data presented in this work concern the accumulation and distribution of Na+, Cl?, K+ and ions, as well as growth and biochemical parameters. Our data show that the development of adaptation reactions in the whole plants in the conditions of high salinity is determined by morphofunctional systems and their interaction.

Identification of a male-specific region(MSR)in Spinacia oleracea

Dioecy,the presence of male and female individual,has evolved independently from hermaphroditism in multiple flowering plant lineages.Spinach,an important leafy vegetable crop worldwide,is a dioecious species with an XY sex-determination system.Although some markers that are fully linked to male-determination locus were identified,the male-specific region(MSR)remained unclear.In this research,five male individuals and five female lines were resequenced to identify the male-specific region.We identified a region(∼21 kb)on chromosome 4(putative sex chromosome)where the five females had a low reads coverage,while the five males had high coverage.A KASP marker,SponR,developed from a single nucleotide polymorphism(SNP)closely linked to the MSR was co-segregating with the sex determination gene in the population of 958 individuals,suggesting that the MSR might be specifically present in male spinach plants.

Isolation and Expression Profiling of a CONSTANS-Like Gene and Two FLOWERING LOCUS T-Like Genes from Spinacia oleracea L.

Spinach (Spinacia oleracea L.) develops leaf rosettes under short-day conditions, and starts reproductive growth including bolting and flowering under long-day conditions. Japanese people prefer Oriental spinach that bolts easily with a shorter photoperiod than European spinach. This is one of the main reasons that Oriental spinach is difficult to grow year-round. In order to understand spinach flowering mechanisms and obtain knowledge for spinach breeding, we isolated one CONSTANS-like (COL) and two FLOWERING LOCUS T (FT) homologs, which are key components of photoperiodic regulation of flowering time, from a Japanese cultivar. The expression of SoCOL1 showed diurnal rhythm with the highest expression at the end of the dark cycle. This diurnal rhythm is similar to the expression of BvCOL1 from sugar beet (Beta vulgaris), whose flower-promoting effect was observed when overexpressed in Arabidopsis. Phylogenetic analysis showed that SoCOL1 is the closest homolog of BvCOL1, suggesting that SoCOL1 is an ortholog of BvCOL1. SoFT1 and SoFT2 are closely related to BvFT1 and BvFT2, respectively. The expression of SoFT1 and SoFT2 were induced in advance of flower bud formation after changing the photoperiod, but the expression level of SoFT1 was much lower than SoFT2. Currently, we are speculating that SoFT2 is a flower-promoting factor of spinach, and that SoFT1 has a role in light signaling because the expression of SoFT1 showed a diurnal rhythm.

海水胁迫对菠菜(Spinacia olerancea L.)叶绿体活性氧和叶绿素代谢的影响

以海水敏感品种圆叶菠菜和耐海水品种‘荷兰3号’为试材,采用水培方法,研究了海水胁迫对菠菜(Spinacia oleranceaL.)叶绿体活性氧(ROS)和叶绿素(Chl)代谢的影响。结果表明,海水胁迫下,2个菠菜品种叶绿体内超氧阴离子(O2.-)产生速率、过氧化氢(H2O2)和丙二醛(MDA)含量显著升高,并且圆叶菠菜的提高幅度大于‘荷兰3号’;圆叶菠菜叶绿体内的超氧化物歧化酶(SOD)、抗坏血酸过氧化物酶(APX)、谷胱甘肽还原酶(GR)活性和抗坏血酸(AsA)、还原型谷胱甘肽(GSH)含量均低于‘荷兰3号’,而过氧化物酶(POD)活性高于‘荷兰3号’;光氧化剂甲基紫精(MV)进一步使2个菠菜品种叶绿体内O2.-产生速率加快、H2O2含量提高、膜质过氧化加重,活性氧清除剂AsA明显降低了菠菜叶绿体内ROS水平,缓解了由MV造成的严重的膜质过氧化伤害。海水胁迫下,2个菠菜品种叶片叶绿素b(Chlb)、叶绿素a(Chla)及其合成前体物质原叶绿素酸(Pchl)、镁原卟啉Ⅸ(Mg-protoIX)、原卟啉Ⅸ(ProtoⅨ)和尿卟啉原Ⅲ(UroⅢ)含量明显降低,而胆色素原(PBG)和δ-氨基酮戊酸(ALA)积累,Chl合成受到阻碍,并且圆叶菠菜的受阻程度大于‘荷兰3号’;MV进一步加剧了这种受阻程度,而AsA部分缓解了由海水胁迫和MV造成的阻碍作用。海水胁迫明显提高了圆叶菠菜叶片叶绿素酶(Chlase)活性而对‘荷兰3号’没有影响,MV处理对圆叶菠菜Chlase活性的影响程度大于‘荷兰3号’,但AsA对2个品种叶片Chlase活性没有明显影响。上述结果说明,海水胁迫下,菠菜叶绿体内ROS与Chl代谢密切相关,不仅通过叶绿体膜的氧化伤害使Chl降解,而且使Chl合成的PBG向UroⅢ转化步骤受阻。耐海水品种‘荷兰3号’叶绿体清除ROS主要通过SOD和AsA-GSH循环系统,清除能力较强,减轻了ROS对叶绿体膜的氧化损伤和Chl合成的受阻程度,并且海水胁迫对其Chlase活性的影响较小;而海水敏感品种圆叶菠菜叶绿体清除ROS主要依赖于SOD和POD,对ROS的清除能力有限,从而导致了ROS大量积累,叶绿体膜的氧化损伤和Chl合成的受阻程度严重,并且海水胁迫显著提高了Chlase活性,加剧了Chl降解。

负离子处理对菠菜贮藏品质的影响研究

目的研究负离子处理时长对菠菜(Spinacia oleracea L.)贮藏品质的影响。方法本实验利用负离子发生器使之在密闭空间中产生负离子,用于菠菜贮藏前的预处理,通过测定贮藏期间菠菜的菌落总数、呼吸速率、质量损失率、色差(ΔE)、丙二醛、总酚含量等品质指标,研究了4种处理时长(0、30、60和90 min)对菠菜贮藏品质的影响。结果负离子处理能抑制菠菜表面微生物的生长,降低微生物对机体造成的侵害,延缓其氧化衰老,同时降低了菠菜呼吸速率,减少营养物质的消耗和叶绿素的分解,并维持较低的质量损失,随着负离子处理时长的增加,对菠菜贮藏过程中的品质保护效果越明显,负离子预处理90 min对菠菜的贮藏品质维护效果最好。可直观体现在菠菜的外观的变化以及色泽的维持。结论负离子合适的处理时长可以有效延长菠菜的货架期,为负离子预处理应用于果蔬保鲜提供了重要依据。

菠菜HD-ZIP Ⅲ基因家族鉴定与表达分析

植物叶片在生长发育过程中,近-远轴极性的建立与维持是叶片正常行使光合效应、呼吸作用、蒸腾作用的结构基础.HD-ZIPⅢ是重要的叶片近轴极性基因,本研究在菠菜(Spinacia oleracea)基因组中鉴定到3个HD-ZIPⅢ基因家族成员,依次命名为SoHB1,SoHB2和SoHB3,并对其蛋白理化性质、保守结构域和进化关系进行了分析.研究结果表明:3个HD-ZIPⅢ蛋白都具有4个保守结构域.共线性分析表明:菠菜SoHB1,SoHB2和SoHB3分别与拟南芥ATHB8,ATREV和ATHB15存在共线性关系.启动子顺式作用元件预测结果表明:菠菜HD-ZIPⅢ家族基因启动子上游包含光响应、逆境胁迫反应、激素响应等顺式作用元件.在不同叶型菠菜种质中的表达模式分析结果表明:单戟型叶片的SoHBs表达量最高;此外,经干旱胁迫处理后,SoHB1,SoHB2和SoHB3的基因表达量普遍下降;对菠菜包括根、茎、薹叶、功能叶、薹叶柄、功能叶柄等部位进行表达分析,发现SoHB1在根部表达最为丰富,SoHB2和SoHB3在薹叶处表达最为丰富.

崇明小菠菜轮作模式的效益及品质分析

为筛选适合崇明小菠菜(Spinacia oleracea)的特色蔬菜轮作模式,以叶菜为对照,比较了崇明小菠菜与香酥芋、红皮土豆、崇明金瓜、崇明白扁豆、崇明香芋和崇明山药为主要轮作作物的6种轮作模式的经济效益,并分析了轮作对崇明小菠菜产量和品质的影响.结果表明:崇明金瓜+鲜食玉米+崇明小菠菜(T2)、崇明白扁豆+鲜食玉米+崇明小菠菜(T3)轮作模式的经济效益在轮作2年周期内均最高,增幅均在对照的20%以上.崇明小菠菜产量增幅在轮作第2年较高,产量最高的轮作模式是崇明红皮土豆+鲜食玉米+崇明小菠菜(T6).各轮作模式下,崇明小菠菜的维生素C、可溶性蛋白质及草酸含量均无显著差异;崇明香芋+崇明小菠菜(T1)轮作模式的硝态氮含量显著高于其他模式;可溶性糖含量在T6轮作模式下含量最高.综合考量经济效益、产量和品质,T2,T3和T6模式可优先应用于崇明小菠菜高效轮作生产.

An in vitro Anti-urolithiasis Activity of a Herbal Formulation:Spinacia oleracea L.and Coriandrum sativum L.

Background:Hard mineral and acid salt deposits that clump together in concentrated urine are known as kidney stones.A complicated chain of physicochemical events,including hyper saturation,nucleation,growth,aggrega-tion,and retention in the urinary tract,leads to urolithiasis,the third most prevalent urinary tract issue.Numerous traditional medications and therapies are less successful in treating urolithiasis and frequently cause recurrences.Objective:The present study is focused on evaluating the anti-urolithiasis activities of the polyherbal formulation prepared from Spinacia oleracea L.and Coriandrum sativum L.seeds.Methods:To evaluating the anti-urolithiasis activities of the polyherbal formulation prepared from S.oleracea and C.sativum seeds against oxalate crystals by employing an in vitro inhibition,aggregation,and nucleation assay,employing a spectrophotometer followed by microscopic observation,and further monitored by egg semiperme-able membrane model,using cystone as a standard drug.Results:In the present work,the alkaloids,glycosides,flavonoids,glycosides,phenols,saponins,steroids,tannins,terpenoids,and triterpenoids were found in the extracts of S.oleracea and C.sativum.The in vitro assays in the present study clearly indicated that all the mixture extracts readily prevented crystal nucleation,growth and ag-gregation.The aqueous extract of the mixture inhibited significant inhibitory potential against the crystallization process.Conclusion:It can be said that the phyto-constituents enriched homemade preparation possibly will be used as a medication for the management of kidney stones by dissolving the oxalate stone in the kidney.

Characterization of Energy_Transduction in Thermal Pretreated Chloroplast from Spinach

Characterization of energy-transduction on die chloroplast thylakoid membranes from spinach (Spinacia oleracca L.) after thermal pretreatment was investigated. The related reactions of energy-transduction in chloroplasts were seriously affected by thermal pretreatment. The results were obtained as following: (1) The rate of cyclic photophosphorylation declined when the pretreatment temperature increased in the range of 25 to 45 degreesC. (2) The thermal pretreatment led to a decrease of the activity of thylakoid membrane-bounded ATPase. (3) Proton uptake of chloroplasts acid the fluorescence quenching of 9-aminoacridine (9-AA) in thylakoid membrane decreased after the thermal pretreatment, but addition of dicyclohexylcarbodiimide (DCCD) could partially restore the fluorescence quenching of 9-AA. (4) Both the rates of fast phase in electrochroism absorption change at 515 nm and the millisecond delayed light emission (ms-DLE) of chloroplast showed a progressive decrease upon raising the temperature of pretreatment. (5) Immunbloting analysis showed that the thermal pretreatment caused the changes of protein content and the electrophoresis mobility of thylakoid membrane-bound ATPase and its alpha -subunit. (6) If the temperature of pretreatment were higher than 33 degreesC, oxygen uptake of PS I -mediated in the samples was rapidly inhibited, but addition of sinapine into the reaction medium could partially restore the ability of oxygen uptake in the samples. These results are briefly discussed in relation to the change of permeability of thylakoid membranes, the dissociation of coupling factor complex as well as accumulation of the radicals in the thylakoid membranes after thermal pretreatment.

菠菜农药残留风险评估探究

为给农药的合理使用和最大残留限量标准的制定提供依据,随机采集多份菠菜样品,对其中的68种农药残留进行检测并得出详细实检测数据。在此基础上,运用慢性、急性膳食摄入风险评估模型,对农药残留的风险进行了全面分析;运用风险矩阵对LD50值、ADI值、该农药残留的食品占总膳食的比例、农药使用频率、是否存在高暴露人群、检测中值分别赋分的方式进行风险排序。结果显示,急性和慢性膳食摄入风险均在可接受范围内。建议对菠菜种植中对农药使用加强监控和指导,加快农药对高危人员多残留的研究,作好菠菜中农药合理使用的指导。

镉胁迫下钝化剂对菠菜生理特征及镉累积的影响

为了探究不同钝化剂在镉(Cd)胁迫下对蔬菜生理特征及Cd累积的影响,以菠菜为研究对象,设置不施钝化剂(CK)、单施纳米羟基磷灰石(nHAP)、单施巯基生物炭(TMB)、配施纳米羟基磷灰石+巯基生物炭(HPTB)4个处理进行盆栽试验。通过测定分析土壤pH、有效态Cd含量及菠菜抗氧化酶活性,阐明施用不同钝化剂对Cd胁迫下菠菜生长及Cd累积的影响。结果表明:施加钝化剂能有效提高菠菜生物量,降低菠菜Cd含量。与CK相比,nHAP、TMB、HPTB处理下地上部和地下部生物量分别增加了90.27%、7.28%、143.75%和72.50%、4.81%、91.71%,Cd含量分别降低了78.19%、6.94%、65.49%和65.98%、39.34%、52.31%;不同钝化剂均能提高土壤pH,降低土壤有效态Cd含量。nHAP、TMB、HPTB处理下土壤pH较CK分别提高了1.36、0.08个和0.76个单位,有效态Cd含量分别降低了83.22%、22.57%和73.70%。nHAP处理对菠菜的降Cd效果最好;施用nHAP能够有效增加抗氧化酶活性,降低丙二醛含量,缓解脂膜过氧化伤害。综上,nHAP可作为缓解Cd污染对菠菜产生生理影响的钝化剂。

镉胁迫下钝化剂对菠菜镉吸收累积及土壤微生物群落结构的影响

为了探究镉(Cd)胁迫下不同钝化剂对菠菜Cd吸收转运及根际微生物群落结构的影响,设置不施钝化剂(CK)、单施纳米羟基磷灰石(nHAP)、单施巯基生物炭(TMB)、配施纳米羟基磷灰石+巯基生物炭(HPTB)4个处理,比较各处理的土壤微生物群落结构、土壤化学性质与菠菜生物量及Cd含量。结果表明,各钝化剂处理下菠菜生物量均增加,Cd含量均降低;不同钝化剂对土壤主要优势菌群的组成没有显著影响,但会改变细菌和真菌的相对丰度。主成分分析表明,nHAP对细菌群落结构影响较大,对真菌群落结构影响较小。冗余分析发现,pH是影响细菌群落结构的主要因子,全磷含量是影响真菌群落结构的主要因子。综上所述,单施nHAP能够促进菠菜生长,降低Cd含量,且对微生物群落结构的影响较小。

菠菜PSY基因家族的鉴定与表达分析

八氢番茄红素合成酶(phytoene synthase,PSY)是植物类胡萝卜素合成途径中的第一个限速酶,对植物的生长发育具有重要作用。为了解菠菜(Spinacia oleracea L.)中PSY基因家族功能,本研究通过生物信息学方法对菠菜PSY基因家族成员的数目、结构、启动子元件、氨基酸特性和基因进化等进行鉴定分析,并利用实时荧光定量PCR技术(RT-qPCR)分析其在菠菜组织中及响应红蓝光比例变化的表达模式,同时测定R1B3(红光∶蓝光=1∶3)和R3B1(红光∶蓝光=3∶1)处理后菠菜总类胡萝卜素含量,进行SoPSYs基因表达量与总类胡萝卜素含量变化的相关性分析。结果显示,菠菜共有4个PSY基因,含有SQS_PSY保守结构域,SoPSY蛋白为亲水性蛋白。菠菜PSY1、PSY2和苋科的甜菜进化关系较近,PSY3、PSY4与番茄PSY3有较近的亲缘关系。菠菜PSY基因启动子序列上存在多种光响应和植物激素相关元件。SoPSY基因具有组织表达特异性,SoPSY1、SoPSY2、SoPSY3仅在叶片中表达,SoPSY4主要在根中表达。菠菜类胡萝卜素含量在R1B3和R3B1处理后均下降。不同基因型菠菜中,各SoPSY基因响应红蓝光比例变化的表达模式具有差异性,其中SoPSY1的表达在栽培类型菠菜中无显著变化,SoPSY2的表达模式在两种类型菠菜中相反,SoPSY3在野生型与栽培型菠菜中表达模式一致,且这3个基因的相对表达水平与总类胡萝卜素含量变化密切相关。本研究为深入了解PSY基因在菠菜中的生物学功能,以及响应红蓝光比例变化的调控机理奠定了基础。

菠菜种质资源耐抽薹性评价与分析

以487份菠菜材料为研究对象,对5个耐抽薹指标进行相关性分析、主成分分析、隶属函数法和聚类分析,综合评价了菠菜的耐抽薹性.研究结果表明:各指标的频数分布基本符合正态分布.菠菜在上海地区抽薹和开花启动的时间为10~13 h,越耐抽薹,抽薹开花启动的时间越长.相关性分析结果表明:抽薹期、开花期、抽薹昼长和开花昼长存在极显著正相关.主成分分析将5个指标转化为2个主成分因子,累计贡献率达到99.5%.用隶属函数法将487份材料划分为5个等级,并筛选出12份极耐抽薹材料.用聚类分析将487份菠菜材料划分为5个类群,其中第Ⅳ类群为极耐抽薹材料,说明隶属函数法和聚类分析均可以有效地评价菠菜的耐抽薹性.最后筛选出10份极耐抽薹材料US175,CS105,CS120,CS121,CS175,CS232,CS253,CS256,CS257和S14,可应用于菠菜耐抽薹材料创制或新品种选育.

不同氮肥水平对菠菜产量和品质的影响

合理施用氮肥对菠菜产量和品质的形成具有重要作用。为探索菠菜生产最佳的氮肥施用方案,以华菠一号菠菜为试验材料,设置6个氮水平处理,研究不同氮肥水平对菠菜产量和品质的影响。结果表明:在磷钾肥等条件一致的前提下,氮肥用量150 kg/hm^(2)的处理菠菜产量最高,达到37 383.45 kg/hm^(2),较不施氮肥的对照增产37.29%;VC含量、可溶性蛋白含量、可溶性固形物含量均最高,分别达到316.78 mg/kg、16.14 mg/g、7.58%,菠菜品质最好。该氮肥施用水平可在生产中进一步推广。

海水胁迫对菠菜叶绿素代谢的影响

以耐海水品种‘荷兰3号’和海水敏感品种‘圆叶菠菜’为试验材料,采用营养液栽培,研究海水胁迫对菠菜叶绿素代谢的影响。结果表明,海水胁迫下,2个菠菜品种叶片的叶绿素a(Chl a)、叶绿素b(Chl b)和总叶绿素含量以及叶绿素合成前体———原叶绿素酸(Pchl)、镁原卟啉IX(Mg-proto IX)、原卟啉IX(Proto IX)和尿卟啉原III(UroIII)含量均明显降低,而胆色素原(PBG)和δ-氨基酮戊酸(ALA)积累,‘圆叶菠菜’的变化幅度大于‘荷兰3号’。海水胁迫下,‘荷兰3号’叶片的叶绿素酶(Chlase)活性无显著变化,胆色素原脱氨酶(PBGD)和尿卟啉原III合酶(UROS)活性在胁迫第3天显著下降,而‘圆叶菠菜’Chlase活性显著上升,PBGD和UROS活性显著下降。研究发现,在海水胁迫条件下,菠菜叶片的叶绿素合成代谢受阻,受阻位点位于PBG→UroIII的转化过程,其中‘圆叶菠菜’的受阻程度大于‘荷兰3号’;耐海水品种‘荷兰3号’叶片叶绿素含量降低主要由叶绿素合成代谢受阻引起,而海水敏感品种‘圆叶菠菜’叶绿素含量的降低则是由叶绿素合成受阻和叶绿素降解共同作用的结果。

菠菜性别相关EST-SSR标记的开发及应用

为了明确菠菜EST序列中SSR的总体特点,开发菠菜EST-SSR引物;为利用EST-SSR引物进行菠菜性别相关特异序列的克隆奠定基础,本文从NCBI上获得1093条EST,利用在线软件SSRIT检测所含SSR序列,并进行分析。共检索出68条SSR序列,分布于64条EST中,检出率为6.22%,包括22种重复基元。其中二核苷酸重复基元的EST-SSR占主导地位,占总SSR数目的32.3%。利用在线引物设计软件Primer3.0设计了7对EST-SSR引物,在适合的PCR反应体系下,分别以雌、雄菠菜DNA基因组为模板,对设计的EST-SSR引物进行筛选,结果显示以EST序列HS097148设计的一对引物从菠菜雌雄基因组中扩增出一条雄性特异的条带,表明通过菠菜EST-SSR引物获得菠菜性别相关特异序列是可行的。

N-Expert专家系统在无公害菠菜生产中的应用

根据无公害蔬菜生长的要求,通过应用德国的氮素专家系统,在引入保证蔬菜的目标产量所要求的土壤最低Nmin残留量(Nmin缓冲值)概念的基础上,以传统的氮素投入为对照,对秋季露地菠菜的氮素施肥进行推荐。这表明在不断优化参数的基础上,N Expert专家系统不仅能够减少肥料的用量,不影响作物产量,而且对提高菠菜的品质和进行蔬菜可持续生产方面有很好的作用。