Tau protein, a microtubule-associated protein, has a high specific expression in neurons and axons. Because traumatic spinal cord injury mainly affects neurons and axons, we speculated that tau protein may be a promis...Tau protein, a microtubule-associated protein, has a high specific expression in neurons and axons. Because traumatic spinal cord injury mainly affects neurons and axons, we speculated that tau protein may be a promising biomarker to reflect the degree of spinal cord injury and prognosis of motor function. In this study, 160 female Sprague-Dawley rats were randomly divided into a sham group, and mild, moderate, and severe spinal cord injury groups. A laminectomy was performed at the T8 level to expose the spinal cord in all groups. A contusion lesion was made with the NYU-MASCIS impactor by dropping a 10 g rod from heights of 12.5 mm(mild), 25 mm(moderate) and 50 mm(severe) upon the exposed dorsal surface of the spinal cord. Tau protein levels were measured in serum and cerebrospinal fluid samples at 1, 6, 12, 24 hours, 3, 7, 14 and 28 days after operation. Locomotor function of all rats was assessed using the Basso, Beattie and Bresnahan locomotor rating scale. Tau protein concentration in the three spinal cord injury groups(both in serum and cerebrospinal fluid) rapidly increased and peaked at 12 hours after spinal cord injury. Statistically significant positive linear correlations were found between tau protein level and spinal cord injury severity in the three spinal cord injury groups, and between the tau protein level and Basso, Beattie, and Bresnahan locomotor rating scale scores. The tau protein level at 12 hours in the three spinal cord injury groups was negatively correlated with Basso, Beattie, and Bresnahan locomotor rating scale scores at 28 days(serum: r =-0.94; cerebrospinal fluid: r =-0.95). Our data suggest that tau protein levels in serum and cerebrospinal fluid might be a promising biomarker for predicting the severity and functional outcome of traumatic spinal cord injury.展开更多
To elucidate the correlation between excitatory amino acids (EAA) and spinal cord injury, we investigat-ed the dynamic changes in excitatory amino acids, aspartate (Asp) and glutamate (Gin ) contents in cerebrospinalf...To elucidate the correlation between excitatory amino acids (EAA) and spinal cord injury, we investigat-ed the dynamic changes in excitatory amino acids, aspartate (Asp) and glutamate (Gin ) contents in cerebrospinalfluid (CSF) of 26 patients with acute spinal cord injury by amino acids autoanalyzer. The results showed that con-tent of glutanlate and aspartate was renlarkably elevated in 24 h after trauma and was related to the seventy of injury. The more severe the spinal cord injured, the more remarkable the content of Asp and Gin in CSF increased.The more pronounced the content of EAA in CSF increased, the worse the patient’s prognosis was. Content of EAA in CSF after spinal cord injury may be an indicator to judge injury extent and prognosis. and provide further support for a potential pathophysiological role of EAA in spinal cord injury.展开更多
The object of this review is to examine the role of TEVAR in causing SCI. The anatomy and physiology of blood flow to the spinal cord is examined. The role of auto regulation of blood flow within the spinal cord is al...The object of this review is to examine the role of TEVAR in causing SCI. The anatomy and physiology of blood flow to the spinal cord is examined. The role of auto regulation of blood flow within the spinal cord is also examined. This review examines the reported results from the scientific literature of the effect of thoracic aortic aneurysm repair on spinal cord blood flow. In the light of the-se findings several conclusions can reasonably be reached. These conclusions are that the development of SCI can reasonably be predicted based on complexity and extent of the TEVAR procedure performed and BP augmentation and CSF drainage can significantly reduce the impact of SCI.展开更多
The evaluation of such novel therapies for acute spinal cord injury in clinical trials is extremely challenging.Our current dependence upon the clinical assessment of neurologic impairment renders many acute SCI patie...The evaluation of such novel therapies for acute spinal cord injury in clinical trials is extremely challenging.Our current dependence upon the clinical assessment of neurologic impairment renders many acute SCI patients ineligible for trials because they are not examinable.Furthermore,the difficulty in predicting neurologic recovery based on the early clinical assessment forces investigators to recruit large cohorts to have sufficient power.Biomarkers that objectively classify injury severity and better predict neurologic outcome would be valuable tools for translational research.As such,the objective of the present review was to describe some of the translational challenges in acute spinal cord injury research and examine the potential utility of neurochemical biomarkers found within cerebrospinal fluid and blood.We focus on published efforts to establish biological markers for accurately classifying injury severity and precisely predict neurological outcome.展开更多
Background Decreasing the intracranial pressure has been advocated as one of the major protective strategies to prevent spinal cord ischemia after endovascular aortic repair. However, the actual changes of cerebrospin...Background Decreasing the intracranial pressure has been advocated as one of the major protective strategies to prevent spinal cord ischemia after endovascular aortic repair. However, the actual changes of cerebrospinal fluid （CSF） pressure and its relation with spinal cord ischemia have been poorly understood. We performed CSF pressure measurements and provisional CSF withdrawal after thoracic endovascular aortic repair, and compared the changes of CSF pressure in high risk patients and in patients with new onset paraplegia and paraparesis.展开更多
背景:同位素标记相对和绝对定量(Isobaric tags for relative and absolute quantitation,i TRAQ)质谱分析技术依据串联质谱中信号离子表达质荷比峰值的不同来研究相关对应蛋白质的信息。目的:建立急性脊髓损大鼠模型,观察其脑脊液差异...背景:同位素标记相对和绝对定量(Isobaric tags for relative and absolute quantitation,i TRAQ)质谱分析技术依据串联质谱中信号离子表达质荷比峰值的不同来研究相关对应蛋白质的信息。目的:建立急性脊髓损大鼠模型,观察其脑脊液差异蛋白谱,从微观分子水平研究急性脊髓损伤后继发性损伤的机制及有效治疗方法。方法:建立SD大鼠急性脊髓损伤模型,取脑脊液应用i TRAQ技术鉴定SD大鼠急性脊髓损伤后脑脊液的差异蛋白质。结果与结论:共鉴定蛋白数722个,差异表达蛋白107个:下调的差异蛋白有63个,上调的差异蛋白数是44个。其中相关神经再生的差异蛋白19个:上调14个,下调5个;调节神经再生的差异蛋白7个。实验中检测到的多种差异蛋白及表达明显的神经再生因子可能作为急性脊髓损伤的生物标记物或可能作为临床管理监测急性脊髓损伤的损伤进程、靶向治疗及评估疗效的强有力证据。展开更多
基金supported by the National Natural Science Foundation of China,No.81671211,81672251(both to HLL)
文摘Tau protein, a microtubule-associated protein, has a high specific expression in neurons and axons. Because traumatic spinal cord injury mainly affects neurons and axons, we speculated that tau protein may be a promising biomarker to reflect the degree of spinal cord injury and prognosis of motor function. In this study, 160 female Sprague-Dawley rats were randomly divided into a sham group, and mild, moderate, and severe spinal cord injury groups. A laminectomy was performed at the T8 level to expose the spinal cord in all groups. A contusion lesion was made with the NYU-MASCIS impactor by dropping a 10 g rod from heights of 12.5 mm(mild), 25 mm(moderate) and 50 mm(severe) upon the exposed dorsal surface of the spinal cord. Tau protein levels were measured in serum and cerebrospinal fluid samples at 1, 6, 12, 24 hours, 3, 7, 14 and 28 days after operation. Locomotor function of all rats was assessed using the Basso, Beattie and Bresnahan locomotor rating scale. Tau protein concentration in the three spinal cord injury groups(both in serum and cerebrospinal fluid) rapidly increased and peaked at 12 hours after spinal cord injury. Statistically significant positive linear correlations were found between tau protein level and spinal cord injury severity in the three spinal cord injury groups, and between the tau protein level and Basso, Beattie, and Bresnahan locomotor rating scale scores. The tau protein level at 12 hours in the three spinal cord injury groups was negatively correlated with Basso, Beattie, and Bresnahan locomotor rating scale scores at 28 days(serum: r =-0.94; cerebrospinal fluid: r =-0.95). Our data suggest that tau protein levels in serum and cerebrospinal fluid might be a promising biomarker for predicting the severity and functional outcome of traumatic spinal cord injury.
文摘To elucidate the correlation between excitatory amino acids (EAA) and spinal cord injury, we investigat-ed the dynamic changes in excitatory amino acids, aspartate (Asp) and glutamate (Gin ) contents in cerebrospinalfluid (CSF) of 26 patients with acute spinal cord injury by amino acids autoanalyzer. The results showed that con-tent of glutanlate and aspartate was renlarkably elevated in 24 h after trauma and was related to the seventy of injury. The more severe the spinal cord injured, the more remarkable the content of Asp and Gin in CSF increased.The more pronounced the content of EAA in CSF increased, the worse the patient’s prognosis was. Content of EAA in CSF after spinal cord injury may be an indicator to judge injury extent and prognosis. and provide further support for a potential pathophysiological role of EAA in spinal cord injury.
文摘The object of this review is to examine the role of TEVAR in causing SCI. The anatomy and physiology of blood flow to the spinal cord is examined. The role of auto regulation of blood flow within the spinal cord is also examined. This review examines the reported results from the scientific literature of the effect of thoracic aortic aneurysm repair on spinal cord blood flow. In the light of the-se findings several conclusions can reasonably be reached. These conclusions are that the development of SCI can reasonably be predicted based on complexity and extent of the TEVAR procedure performed and BP augmentation and CSF drainage can significantly reduce the impact of SCI.
基金provided by the Rick Hansen InstituteMichael Smith Foundation for Health Research+1 种基金Craig Neilsen Foundationsupported by MITACS
文摘The evaluation of such novel therapies for acute spinal cord injury in clinical trials is extremely challenging.Our current dependence upon the clinical assessment of neurologic impairment renders many acute SCI patients ineligible for trials because they are not examinable.Furthermore,the difficulty in predicting neurologic recovery based on the early clinical assessment forces investigators to recruit large cohorts to have sufficient power.Biomarkers that objectively classify injury severity and better predict neurologic outcome would be valuable tools for translational research.As such,the objective of the present review was to describe some of the translational challenges in acute spinal cord injury research and examine the potential utility of neurochemical biomarkers found within cerebrospinal fluid and blood.We focus on published efforts to establish biological markers for accurately classifying injury severity and precisely predict neurological outcome.
文摘Background Decreasing the intracranial pressure has been advocated as one of the major protective strategies to prevent spinal cord ischemia after endovascular aortic repair. However, the actual changes of cerebrospinal fluid （CSF） pressure and its relation with spinal cord ischemia have been poorly understood. We performed CSF pressure measurements and provisional CSF withdrawal after thoracic endovascular aortic repair, and compared the changes of CSF pressure in high risk patients and in patients with new onset paraplegia and paraparesis.
文摘背景:同位素标记相对和绝对定量(Isobaric tags for relative and absolute quantitation,i TRAQ)质谱分析技术依据串联质谱中信号离子表达质荷比峰值的不同来研究相关对应蛋白质的信息。目的:建立急性脊髓损大鼠模型,观察其脑脊液差异蛋白谱,从微观分子水平研究急性脊髓损伤后继发性损伤的机制及有效治疗方法。方法:建立SD大鼠急性脊髓损伤模型,取脑脊液应用i TRAQ技术鉴定SD大鼠急性脊髓损伤后脑脊液的差异蛋白质。结果与结论:共鉴定蛋白数722个,差异表达蛋白107个:下调的差异蛋白有63个,上调的差异蛋白数是44个。其中相关神经再生的差异蛋白19个:上调14个,下调5个;调节神经再生的差异蛋白7个。实验中检测到的多种差异蛋白及表达明显的神经再生因子可能作为急性脊髓损伤的生物标记物或可能作为临床管理监测急性脊髓损伤的损伤进程、靶向治疗及评估疗效的强有力证据。
