By a sequentially selective extraction procedure, polysaccharides (PS) from cyanobacterium Spirulina (Arthrospira) maxima that can be bio functionally healthy components were fractionated in four parts including the c...By a sequentially selective extraction procedure, polysaccharides (PS) from cyanobacterium Spirulina (Arthrospira) maxima that can be bio functionally healthy components were fractionated in four parts including the culture medium (PSCM), the external layers of the cell (PSEL), the cell wall (PSCW) and storage granules (PSSG). The four fractionated parts of polysaccharides were characterized by gas liquid chromatography (GLC) equipped with FID and fused silica capillary column (15 m×0.53 mm i. d.). The contents of PSCM, PSEL, PSCW and PSSG were about 2.0%, 2.6%, 10.0% and 52.0% to cell dry matter, respectively. Glucose was almost the only monosaccharide in PSSG and PSCW and most predominant in PSEL, while in PSCM, xylose, rhamnose and glucose were the main ones. Two uronic acids represented by glucuronic acid and galacturoic acid, six neutral monosaccharides including fucose, rhamnose, xylose, mannose, galactose and glucose, and two possible unidentified sugars were found in PSEL and PSCM. These data are valuable for the selective productions of high added value sugars from Spirulina .展开更多
A tubular photobioreactor was constructed with glass tubing of 5 4mm bore. The high density culture of Spirulina platensis was tested and the biomass concentration had gained up to 12 4g/L. The research shows the phot...A tubular photobioreactor was constructed with glass tubing of 5 4mm bore. The high density culture of Spirulina platensis was tested and the biomass concentration had gained up to 12 4g/L. The research shows the photobioreactor productivity can be influenced significantly by incident light intensity and light/dark ratio. Along with the increasing of light/dark ratio, the productivity per culture volume increased at first, then the light/dark ratio saturation phenomenon occurred; and the productivity per illuminated area increased at first, after that it decreased. There is an optimum light/dark ratio, at which the productivity per illuminated area is optimized. The dynamic viscosity measurement of the culture liquid shows non Newtonian behavior and it can be described by power law equation.展开更多
文摘By a sequentially selective extraction procedure, polysaccharides (PS) from cyanobacterium Spirulina (Arthrospira) maxima that can be bio functionally healthy components were fractionated in four parts including the culture medium (PSCM), the external layers of the cell (PSEL), the cell wall (PSCW) and storage granules (PSSG). The four fractionated parts of polysaccharides were characterized by gas liquid chromatography (GLC) equipped with FID and fused silica capillary column (15 m×0.53 mm i. d.). The contents of PSCM, PSEL, PSCW and PSSG were about 2.0%, 2.6%, 10.0% and 52.0% to cell dry matter, respectively. Glucose was almost the only monosaccharide in PSSG and PSCW and most predominant in PSEL, while in PSCM, xylose, rhamnose and glucose were the main ones. Two uronic acids represented by glucuronic acid and galacturoic acid, six neutral monosaccharides including fucose, rhamnose, xylose, mannose, galactose and glucose, and two possible unidentified sugars were found in PSEL and PSCM. These data are valuable for the selective productions of high added value sugars from Spirulina .
文摘A tubular photobioreactor was constructed with glass tubing of 5 4mm bore. The high density culture of Spirulina platensis was tested and the biomass concentration had gained up to 12 4g/L. The research shows the photobioreactor productivity can be influenced significantly by incident light intensity and light/dark ratio. Along with the increasing of light/dark ratio, the productivity per culture volume increased at first, then the light/dark ratio saturation phenomenon occurred; and the productivity per illuminated area increased at first, after that it decreased. There is an optimum light/dark ratio, at which the productivity per illuminated area is optimized. The dynamic viscosity measurement of the culture liquid shows non Newtonian behavior and it can be described by power law equation.
