The objective of this study was to measure protein quality parameters (PPV, NPU and NPUstd) of chicken diets with complete substitution of soybean meal (SBM) by Spirulina meal (SM) or partly defatted Hermetia meal (HM...The objective of this study was to measure protein quality parameters (PPV, NPU and NPUstd) of chicken diets with complete substitution of soybean meal (SBM) by Spirulina meal (SM) or partly defatted Hermetia meal (HM). N balance experiments were based on the quantitative excreta collection method, divided into starter period (10 - 20 d) and grower period (25 - 35 d). The study utilized 70 all male meat type chicken (Ross 308). Data assessment applied the exponential N utilization model of the “Goettingen approach”. The control diet was based on wheat, corn and SBM. In four experimental diets SBM was completely substituted by SM or HM, but fortified with feed amino acids (AA) both on a basic level of supplementation (Lys and Met added equal to the control diet) and on an extended level (Lys, Met, Thr, Arg, Val, Ile, His added). At a basic level of AA supplementation, complete replacement of SBM by SM or HM in chicken diets depressed dietary protein quality significantly (p 0.05). However, the extended level of AA supplementation improved protein quality parameters of the diets with both of the alternative proteins significantly (p < 0.05), but still generally not on par with the control diet. The observed responses were accentuated when the well-known effect of N intake on protein utilization was eliminated through the standardization of N intake by application of the “Goettingen approach”.展开更多
蛋白-多糖乳液凝胶作为活性物的包载体系,具有载量高、生物相容性高、稳定性好等优点。该文以螺旋藻分离蛋白(Spirulina protein isolate,SPI)和透明质酸(hyaluronic acid,HA)为乳化剂和凝胶基质,制备了不同油相体积分数的SPI乳液、SPI...蛋白-多糖乳液凝胶作为活性物的包载体系,具有载量高、生物相容性高、稳定性好等优点。该文以螺旋藻分离蛋白(Spirulina protein isolate,SPI)和透明质酸(hyaluronic acid,HA)为乳化剂和凝胶基质,制备了不同油相体积分数的SPI乳液、SPI-HA乳液和乳液凝胶,表征了乳液的微观结构和粒径,分析了不同油相体积分数对乳液凝胶形成的影响,并探究了乳液凝胶对β-胡萝卜素的高温保护效果。结果表明,SPI乳液随着HA的引入,乳液粒径明显降低;乳液凝胶结构则随着油相体积分数的增加越来越致密;当油相体积分数为60%时,乳液凝胶的屈服应力、储能模量以及表观黏度均高于SPI乳液和SPI-HA乳液;乳液凝胶对70℃,8 h下β-胡萝卜素的保留率高达(78.83±2.75)%。综上所述,SPI-HA乳液凝胶在食品领域中具有良好的应用前景。展开更多
In this paper we report that CeCl3 could accelerate the synthesis of chlorophyll a and protein in spirulina platensis. and enhance the activity of oxygen evolution. We also found that the accelerations of CeCl3 in spi...In this paper we report that CeCl3 could accelerate the synthesis of chlorophyll a and protein in spirulina platensis. and enhance the activity of oxygen evolution. We also found that the accelerations of CeCl3 in spirulina platensis culture solution of low NaHCO3 were more than in culture solution of high NaHCO3, therefore it was related to the concentration of NaHCO3 The absorption spectrum and low temperature (77 K) fluorescence emission spectrum of spirulina platensis with CeCl3 treated were significantly different from their controls,i.e.its absorption peak of phycocyanin at 620 nm was higher than that of the control.its absorption peak at the long wave region migrated from 676 to 680 nm, and its fluorecence emission ratios (730 nm/685～695 nm) of photosystem I(PSI) to photosystem Ⅱ(PS Ⅱ) were also higher than those of the control; the pitch of the spiral algal body became shorter, and induced structural changes too.展开更多
实验以异育银鲫"中科3号"幼鱼(3.2±0.5)g为实验对象,研究螺旋藻粉蛋白替代饲料鱼粉蛋白(0、20%、40%、60%、80%和100%)对鱼摄食、生长、饲料利用和蛋白沉积的影响。实验设计了6种等氮(38%)等脂(10%)等能(16.50 k J)的饲...实验以异育银鲫"中科3号"幼鱼(3.2±0.5)g为实验对象,研究螺旋藻粉蛋白替代饲料鱼粉蛋白(0、20%、40%、60%、80%和100%)对鱼摄食、生长、饲料利用和蛋白沉积的影响。实验设计了6种等氮(38%)等脂(10%)等能(16.50 k J)的饲料,每处理3个重复。采用室内循环水养殖系统,表观饱食投喂。实验水温为(28±3)℃,实验共持续60d。结果发现:随藻粉替代比例的增加,鱼摄食率显著上升;饲料效率则呈显著下降趋势;特定生长率在20%替代组与鱼粉组无显著差异(P>0.05),其他各替代组均显著低于鱼粉组(P<0.05);蛋白沉积率在20%、60%替代时与鱼粉组无显著差异(P>0.05),其他替代组显著低于鱼粉组(P<0.05);肥满度在各替代组均显著低于鱼粉组(P<0.05);鱼体蛋白随藻粉替代比例的增加呈现先持平后降低的趋势,鱼体脂肪则呈先降低后升高的变化;20%、40%和60%替代组全鱼灰分均高于全鱼粉组;肠道消化酶(胰蛋白酶、脂肪酶和α-淀粉酶)在替代组和对照组间均无显著差异(P>0.05);干物质表观消化率随藻粉蛋白替代比例的增加而呈显著上升,蛋白的表观消化率则呈显著下降趋势,总磷表观消化率在20%替代时最低,在100%替代时最高。综合特定生长率和蛋白沉积率情况,当鲫幼鱼饲料中鱼粉含量约为50%,且鱼粉为唯一蛋白源时,螺旋藻粉蛋白可替代饲料中20%鱼粉蛋白。展开更多
文摘The objective of this study was to measure protein quality parameters (PPV, NPU and NPUstd) of chicken diets with complete substitution of soybean meal (SBM) by Spirulina meal (SM) or partly defatted Hermetia meal (HM). N balance experiments were based on the quantitative excreta collection method, divided into starter period (10 - 20 d) and grower period (25 - 35 d). The study utilized 70 all male meat type chicken (Ross 308). Data assessment applied the exponential N utilization model of the “Goettingen approach”. The control diet was based on wheat, corn and SBM. In four experimental diets SBM was completely substituted by SM or HM, but fortified with feed amino acids (AA) both on a basic level of supplementation (Lys and Met added equal to the control diet) and on an extended level (Lys, Met, Thr, Arg, Val, Ile, His added). At a basic level of AA supplementation, complete replacement of SBM by SM or HM in chicken diets depressed dietary protein quality significantly (p 0.05). However, the extended level of AA supplementation improved protein quality parameters of the diets with both of the alternative proteins significantly (p < 0.05), but still generally not on par with the control diet. The observed responses were accentuated when the well-known effect of N intake on protein utilization was eliminated through the standardization of N intake by application of the “Goettingen approach”.
文摘蛋白-多糖乳液凝胶作为活性物的包载体系,具有载量高、生物相容性高、稳定性好等优点。该文以螺旋藻分离蛋白(Spirulina protein isolate,SPI)和透明质酸(hyaluronic acid,HA)为乳化剂和凝胶基质,制备了不同油相体积分数的SPI乳液、SPI-HA乳液和乳液凝胶,表征了乳液的微观结构和粒径,分析了不同油相体积分数对乳液凝胶形成的影响,并探究了乳液凝胶对β-胡萝卜素的高温保护效果。结果表明,SPI乳液随着HA的引入,乳液粒径明显降低;乳液凝胶结构则随着油相体积分数的增加越来越致密;当油相体积分数为60%时,乳液凝胶的屈服应力、储能模量以及表观黏度均高于SPI乳液和SPI-HA乳液;乳液凝胶对70℃,8 h下β-胡萝卜素的保留率高达(78.83±2.75)%。综上所述,SPI-HA乳液凝胶在食品领域中具有良好的应用前景。
文摘In this paper we report that CeCl3 could accelerate the synthesis of chlorophyll a and protein in spirulina platensis. and enhance the activity of oxygen evolution. We also found that the accelerations of CeCl3 in spirulina platensis culture solution of low NaHCO3 were more than in culture solution of high NaHCO3, therefore it was related to the concentration of NaHCO3 The absorption spectrum and low temperature (77 K) fluorescence emission spectrum of spirulina platensis with CeCl3 treated were significantly different from their controls,i.e.its absorption peak of phycocyanin at 620 nm was higher than that of the control.its absorption peak at the long wave region migrated from 676 to 680 nm, and its fluorecence emission ratios (730 nm/685～695 nm) of photosystem I(PSI) to photosystem Ⅱ(PS Ⅱ) were also higher than those of the control; the pitch of the spiral algal body became shorter, and induced structural changes too.
文摘实验以异育银鲫"中科3号"幼鱼(3.2±0.5)g为实验对象,研究螺旋藻粉蛋白替代饲料鱼粉蛋白(0、20%、40%、60%、80%和100%)对鱼摄食、生长、饲料利用和蛋白沉积的影响。实验设计了6种等氮(38%)等脂(10%)等能(16.50 k J)的饲料,每处理3个重复。采用室内循环水养殖系统,表观饱食投喂。实验水温为(28±3)℃,实验共持续60d。结果发现:随藻粉替代比例的增加,鱼摄食率显著上升;饲料效率则呈显著下降趋势;特定生长率在20%替代组与鱼粉组无显著差异(P>0.05),其他各替代组均显著低于鱼粉组(P<0.05);蛋白沉积率在20%、60%替代时与鱼粉组无显著差异(P>0.05),其他替代组显著低于鱼粉组(P<0.05);肥满度在各替代组均显著低于鱼粉组(P<0.05);鱼体蛋白随藻粉替代比例的增加呈现先持平后降低的趋势,鱼体脂肪则呈先降低后升高的变化;20%、40%和60%替代组全鱼灰分均高于全鱼粉组;肠道消化酶(胰蛋白酶、脂肪酶和α-淀粉酶)在替代组和对照组间均无显著差异(P>0.05);干物质表观消化率随藻粉蛋白替代比例的增加而呈显著上升,蛋白的表观消化率则呈显著下降趋势,总磷表观消化率在20%替代时最低,在100%替代时最高。综合特定生长率和蛋白沉积率情况,当鲫幼鱼饲料中鱼粉含量约为50%,且鱼粉为唯一蛋白源时,螺旋藻粉蛋白可替代饲料中20%鱼粉蛋白。
