Effects of NGF and TrkA on GAP-43^+ nerve regeneration in rat autotransplanted splenic tissue

Objective:To study the time-course of the regeneration of GAP-43+ nerve, and the effects of NGF and TrkA on this process. Methods: Adult Wistar rats underwent splenectomy and splenic autotransplantation, or sham-operation. On day 7, 14, 30, 60, 90, 120, and 180 after surgery, the density of GAP-43+ nerve fibers in spleen tissues were measured with the immunohistochemistry followed by computer image analysis. The expressions of GAP-43, NGF and TrkA were determined with in situ hybrdization, and their mRNA levels were detected with RT-PCR and image analysis qualification. Results: (1) The GAP-43+ nerve fibers began their regeneration on 30 d after operation and extended from greater omentum into splenic autotransplants. Density of the nerve fibers gradually became greater and almost normal 180 d after operation. (2) In splenic autografts, the mRNA expression of GAP-43, NGF and TrkA appeared on day 30 after the operation, gradually reached the peak on day 90. Conclusion: The renascent GAP-43+ nerve fibers may come from the greater omentum packaging the splenic autografts and NGF and TrkA can promote the nerval regeneration in the autotransplant spleen tissues.

大鼠自体脾组织网膜囊内移植抗肿瘤功能的实验探讨

目的 :为了探讨自体脾组织片移植对荷瘤机体抗肿瘤免疫功能影响。方法 :我们用 S1 80 瘤株制备了荷瘤动物模型 ,以模拟脾切除组和脾切除组为对照 ,以巨噬细胞吞噬功能、外周血 T淋巴细胞亚群测定、血清白细胞介素 - 2含量测定、肿瘤重量测定 ,对自体脾组织移植的抗肿瘤作用进行观察。结果 :自体脾组织移植组的抗肿瘤功能与模拟脾切除组相比 ,无显著差异(P>0 .0 5 ) ;与脾切除组相比差异显著 (P <0 .0 5 )。结论 :自体移植脾组织存活后 ,其抗肿瘤免疫功能与正常脾功能相比无显著差异。自体脾组织移植是脾切除后弥补脾功能的有效办法。