TaSC(Triticum asetivum L.salt-tolerance related gene,Gen Bank登录号为AY956330)是从小麦耐盐突变体RH8706-49中克隆的高盐诱导表达的耐盐相关基因。以该基因编码的蛋白质TaSC为诱饵,运用分裂泛素酵母双杂交技术从小麦cDNA表达文库...TaSC(Triticum asetivum L.salt-tolerance related gene,Gen Bank登录号为AY956330)是从小麦耐盐突变体RH8706-49中克隆的高盐诱导表达的耐盐相关基因。以该基因编码的蛋白质TaSC为诱饵,运用分裂泛素酵母双杂交技术从小麦cDNA表达文库中钓取互作蛋白质,筛选到一个编码小麦未知功能蛋白质的基因(GenBank登录号为AK336035),命名为TaSCIP1(TaSC interaction protein 1)。双分子荧光互补(BiFC)实验证实TaSCIP1与TaSC存在互作。该互作蛋白的分离有利于进一步研究TaSC基因的耐盐机制。展开更多
MAP kinase-interacting kinase-2 (Mnk2) is one of the downstream kinasesactivated by MAP kinases. It phosphorylates the eukaryotic initiation factor 4E (elF4E), althoughthe role of elF4E phosphorylation and the role of...MAP kinase-interacting kinase-2 (Mnk2) is one of the downstream kinasesactivated by MAP kinases. It phosphorylates the eukaryotic initiation factor 4E (elF4E), althoughthe role of elF4E phosphorylation and the role of Mnk2 in the process of proteintranslation are notwell understood. Except for elF4E, other physiological substrates of Mnk2 are still unidentified. Tolook for these unidentified substrates and to reveal the physiological function of Mnk2, weperformed a yeast two-hybrid screening with Mnk2 as the bait. The results demonstrated Mnk2 couldinteract with VHL (von Hippel-Lindau tumor suppressor), Rbx1 (ring-box1) and Cul2 (Cullin2) proteinsin yeast cells. Furthermore, we validated the interaction between Mnk2 and VHL proteins inmammalian cells by co-immunoprecipitation analysis. Because the three proteins VHL, Rbx1 and Cul2are all components of the CBC^(VHL) ubiquitin ligase E3 complex, it has been shown that Mnk2 caninteract with CBC^(VHL) complex, and is probably one of the newsubstrates of the CBC^(VHL) complex.Furthermore, during the interaction of Mnk2 with von Hippel-Lindau (VHL) tumor suppressor- bindingprotein 1 (VBP1), it appears that Mnk2 also joins to modulate cell shape as VBP1 plays an importantrole in the process of the maturation of the cytoskeleton and in the process of morphogenesis.展开更多
文摘TaSC(Triticum asetivum L.salt-tolerance related gene,Gen Bank登录号为AY956330)是从小麦耐盐突变体RH8706-49中克隆的高盐诱导表达的耐盐相关基因。以该基因编码的蛋白质TaSC为诱饵,运用分裂泛素酵母双杂交技术从小麦cDNA表达文库中钓取互作蛋白质,筛选到一个编码小麦未知功能蛋白质的基因(GenBank登录号为AK336035),命名为TaSCIP1(TaSC interaction protein 1)。双分子荧光互补(BiFC)实验证实TaSCIP1与TaSC存在互作。该互作蛋白的分离有利于进一步研究TaSC基因的耐盐机制。
文摘MAP kinase-interacting kinase-2 (Mnk2) is one of the downstream kinasesactivated by MAP kinases. It phosphorylates the eukaryotic initiation factor 4E (elF4E), althoughthe role of elF4E phosphorylation and the role of Mnk2 in the process of proteintranslation are notwell understood. Except for elF4E, other physiological substrates of Mnk2 are still unidentified. Tolook for these unidentified substrates and to reveal the physiological function of Mnk2, weperformed a yeast two-hybrid screening with Mnk2 as the bait. The results demonstrated Mnk2 couldinteract with VHL (von Hippel-Lindau tumor suppressor), Rbx1 (ring-box1) and Cul2 (Cullin2) proteinsin yeast cells. Furthermore, we validated the interaction between Mnk2 and VHL proteins inmammalian cells by co-immunoprecipitation analysis. Because the three proteins VHL, Rbx1 and Cul2are all components of the CBC^(VHL) ubiquitin ligase E3 complex, it has been shown that Mnk2 caninteract with CBC^(VHL) complex, and is probably one of the newsubstrates of the CBC^(VHL) complex.Furthermore, during the interaction of Mnk2 with von Hippel-Lindau (VHL) tumor suppressor- bindingprotein 1 (VBP1), it appears that Mnk2 also joins to modulate cell shape as VBP1 plays an importantrole in the process of the maturation of the cytoskeleton and in the process of morphogenesis.
