A water soluble, (1 -->6)-branched, (1 -->4) linked D-glucan (LB-B1), [alpha](D)(21) = +174.2 degrees (c 0.87, H2O), was obtained from a hot-water extract of the sporoderm-broken spores of Ganoderma lucidun (Fr....A water soluble, (1 -->6)-branched, (1 -->4) linked D-glucan (LB-B1), [alpha](D)(21) = +174.2 degrees (c 0.87, H2O), was obtained from a hot-water extract of the sporoderm-broken spores of Ganoderma lucidun (Fr.) Karst by HPSEC, with 0.001 mol/L sodium hydroxide as the eluant, the molecular weight (Mw) of LB-B1 was estimated to be 9.3 x 10(3). From the results of total hydrolysis, methylation analysis, acetolysis and 1D, 2D-NMR experimentation, it was concluded that LB-B1 was composed of repeating units with the following structure: alpha -D-Glc(p)-(1 -->6)-alpha -D-Glc(p)-(1 -->6)-alpha -D-Glc(p) 1 down arrow 6 (-->4)-alpha -D-Glc(p)-(1 -->4)-alpha -D-Glc(p)-(1 -->4)-alpha -D-Glc(p)-(1 -->4)-alpha -D-Glc(p)-(1 -->4-)-alpha -D-Glc(p)-(1 -->)(n)展开更多
The spores of Ganoderma lucidum were ground and broken to ultrafine particles by high speed centrifugal shearing（HSCS） pulverizer. The characteristics of Ganoderma lucidum spores were analyzed by scanning electron m...The spores of Ganoderma lucidum were ground and broken to ultrafine particles by high speed centrifugal shearing（HSCS） pulverizer. The characteristics of Ganoderma lucidum spores were analyzed by scanning electron microscope （SEM）, Fourier transform infrared spectrophotometry （FTIR）. Ultraviolet-visible pectrophotometer was used to determine the extraction ratio of aqueous solubility polysaccharide between the raw and broken spores. The immunological function on the mice before and after the breaking of spores wan investigated. The experimental results show that after being ground, the sporoderm-broken ratio reachs 100%, the original active ingredients of ganoderma lucidum spores do not change, and the extraction ratio of aqueous solubility polysaccharide is greatly increased by 40.08%. The broken spores show much higher immunological activity comparing with original spores of Ganoderma lucidum.展开更多
文摘A water soluble, (1 -->6)-branched, (1 -->4) linked D-glucan (LB-B1), [alpha](D)(21) = +174.2 degrees (c 0.87, H2O), was obtained from a hot-water extract of the sporoderm-broken spores of Ganoderma lucidun (Fr.) Karst by HPSEC, with 0.001 mol/L sodium hydroxide as the eluant, the molecular weight (Mw) of LB-B1 was estimated to be 9.3 x 10(3). From the results of total hydrolysis, methylation analysis, acetolysis and 1D, 2D-NMR experimentation, it was concluded that LB-B1 was composed of repeating units with the following structure: alpha -D-Glc(p)-(1 -->6)-alpha -D-Glc(p)-(1 -->6)-alpha -D-Glc(p) 1 down arrow 6 (-->4)-alpha -D-Glc(p)-(1 -->4)-alpha -D-Glc(p)-(1 -->4)-alpha -D-Glc(p)-(1 -->4)-alpha -D-Glc(p)-(1 -->4-)-alpha -D-Glc(p)-(1 -->)(n)
基金the National Natural Science Foundation of China(No.50272047)Ministry of Education of China(No.704034)
文摘The spores of Ganoderma lucidum were ground and broken to ultrafine particles by high speed centrifugal shearing（HSCS） pulverizer. The characteristics of Ganoderma lucidum spores were analyzed by scanning electron microscope （SEM）, Fourier transform infrared spectrophotometry （FTIR）. Ultraviolet-visible pectrophotometer was used to determine the extraction ratio of aqueous solubility polysaccharide between the raw and broken spores. The immunological function on the mice before and after the breaking of spores wan investigated. The experimental results show that after being ground, the sporoderm-broken ratio reachs 100%, the original active ingredients of ganoderma lucidum spores do not change, and the extraction ratio of aqueous solubility polysaccharide is greatly increased by 40.08%. The broken spores show much higher immunological activity comparing with original spores of Ganoderma lucidum.
