This study investigated the effect of different dielectric barrier discharge cold plasma(DBD-CP) treatment voltages on the microbiological growth and quality characteristics of braised chicken during storage at(4 ...This study investigated the effect of different dielectric barrier discharge cold plasma(DBD-CP) treatment voltages on the microbiological growth and quality characteristics of braised chicken during storage at(4 ± 1) ℃. Argon-packed samples were subjected to DBD-CP treatment for 3 min at voltages of 50, 60 and 70 k V. As a result, there was no significant(P > 0.05) difference in L*, a*, b* and p H of braised chicken between control and treatment groups at the same storage time. However, the development of lipid oxidation in DBD-CP treatment samples was slower than that in air-packed samples. Compared to air-packed samples, the reduction of total viable count, yeasts and molds, lactic acid bacteria and Pseudomonads spp. in 70 k V treated samples on day 15 were 3.21, 2.41, 2.44 and 1.96 lg(CFU/g), respectively. The results indicate that DBD-CP treatment could extend the shelf life of braised chicken without negative impacts on quality characteristics.展开更多
Aim: To confirm the stability of exogenous genes in the generation of transgenic chickens using ejaculated chicken sperm, the deoxyribonuclease (DNase) activity was evaluated in the seminal plasma of ejaculated semen ...Aim: To confirm the stability of exogenous genes in the generation of transgenic chickens using ejaculated chicken sperm, the deoxyribonuclease (DNase) activity was evaluated in the seminal plasma of ejaculated semen and the stability of DNA was examined by adding lipofection reagents. Methods: A PCR fragment (249 bp) of pEGFPN-1 vector was used as the DNA substrate and was incubated with the seminal plasma at 40 ℃ for 30 min. Then, the whole reaction solution was subjected to agarose gel electrophoresis and the DNA size was evaluated under UV light. Results: The DNA substrate was completely diminished after incubation with seminal plasma. However, the substrate was intact after incubation with heat-treated seminal plasma or incubation with seminal plasma in the presence of 0.5 mmol/L -5 mmol/L EDTA. The substrate was stabilized in the seminal plasma by the addition of commercially available lipofection reagents. Conclusion: The DNase activity is present in the seminal plasma of ejaculated chicken semen. However, DNA is stable in the liposomal-DNA complex.展开更多
The chickens used in this study were from a pure line of brown shell egg-type (YAFA) female grandparents of 17-week-old. Plasma very low density lipoprotein (VLDL) concentration was measured with turbidimetric assay. ...The chickens used in this study were from a pure line of brown shell egg-type (YAFA) female grandparents of 17-week-old. Plasma very low density lipoprotein (VLDL) concentration was measured with turbidimetric assay. The experimental results showed that there was a close relationship between plasma VLDL concentration and egg composition traits, and a positive phenotypic correlation trend was found between 29-week plasma VLDL concentration and 44-week yolk weight, and the genetic correlation coefficient was 0.234. Both the phenotypic and genetic correlations between 29-week plasma VLDL concentration and albumen weight were negative. Significant positive phenotypie correlations were, however, observed between yolk ratio, or the yolk/albumen ratio and 29-week plasma VLDL concentration, and their genetic correlation coefficients were 0.250 and 0.223, respectively.展开更多
The chickens studied were from a pure line of brown shell egg-type (YAFA) female grandparents of 17-week-old. Plasima very low density lipoprotein (VLDL) concentration was measured with turbidimetric assay. The experi...The chickens studied were from a pure line of brown shell egg-type (YAFA) female grandparents of 17-week-old. Plasima very low density lipoprotein (VLDL) concentration was measured with turbidimetric assay. The experiment results showed that the phenotypic correlations between 29-week or 50-week plasma VLDL concentration and egg production (EP) were positive in the early stage of laying period, but those in the latter stage were negative. Selection for low plasma VLDL concentration will decrease the EP in the early stage of laying period but increase the one in the latter stage. There was a significant negative phenotypic correlation between the age at first egg (AFE) and 18-week body weight (BW). The phenotypic correlation between 29-week plasma VLDL concentration and the AFE was negative. AFE was influenced by BW and body fatness as well. There was significant positive phenotypic correlation between plasima VLDL concentration and body weights (BWs) at the same stage in laying period and the phenotypic correlation was also positive between 29-week plasma VLDL concentration and the BW at middle stage of laying period, indicating that selection for low plasma VLDL concentration would reduce BWs at various stages to different degree.展开更多
Blood samples were taken from birds,from a commercial line of broiler chicken,being in a fully fed state and in fasting state for 2,4,6 hours respectively at 49 d of age and plasma very low density lipoprotein(VLDL) c...Blood samples were taken from birds,from a commercial line of broiler chicken,being in a fully fed state and in fasting state for 2,4,6 hours respectively at 49 d of age and plasma very low density lipoprotein(VLDL) concentration was measured with turbidimetric assay.The experiment results showed that the variation coefficients of plasma VLDL concentration mersured at four different times were 58.11%,74.39%,69.70%,47.83%,respectively for male broilers,and 41.82%,60.87%.59.26%,50.0%for female broilers respectively.Plasma VLDL concentration decreased with the fasting time.There was a significant positive phenotypic correlation between abdominal fat weight,percentage of abdominal fat and plasma VLDL concentration for the birds being in fully feeding state,and the correlation decreased or no longer existed for the bired being in fasting state.The conclusion was that for practically application of plasma VLDL content selected in broiler chicken breeding,blood samples should be collected from birds being in feeding state.展开更多
Two kinds of TiO2-ZnO nano-compounded powders aggregated by spray-drying process and evaporation method were used to deposit photocatalytic coatings by atmospheric plasma spraying technique. The phase compositions, mo...Two kinds of TiO2-ZnO nano-compounded powders aggregated by spray-drying process and evaporation method were used to deposit photocatalytic coatings by atmospheric plasma spraying technique. The phase compositions, morphologies of the agglomerated TiO2-ZnO powders and the as-sprayed coating were characterized using X-ray diffraction (XRD) and field emission scanning electron microscope (FESEM) respectively. Furthermore, roughness measurements were carried out on their surfaces of the plasma sprayed TiO2-ZnO nano-comPounded coatings. Compared with the TiO2ZnO nano-compounded coating deposited from the spray-dried powder, it was found that the TiO2-ZnO nano-compounded coating deposited from the evaporated powder possesses higher anatase phase. It is ascribed to the existing of partially melted or non-melted microstructure in the TiO2-ZnO coating deposited from the evaporated powder. The partially-meltedor non-melted microstructure was retained from the starting agglomerated powder. This microstructure is beneficial to improve the photocatalytic properties of plasma sprayed TiO2-ZnO nano-compounded coatings.展开更多
基金financially supported by the Key R&D Program(Modern Agriculture)of Jiangsu Province(BE2019308)。
文摘This study investigated the effect of different dielectric barrier discharge cold plasma(DBD-CP) treatment voltages on the microbiological growth and quality characteristics of braised chicken during storage at(4 ± 1) ℃. Argon-packed samples were subjected to DBD-CP treatment for 3 min at voltages of 50, 60 and 70 k V. As a result, there was no significant(P > 0.05) difference in L*, a*, b* and p H of braised chicken between control and treatment groups at the same storage time. However, the development of lipid oxidation in DBD-CP treatment samples was slower than that in air-packed samples. Compared to air-packed samples, the reduction of total viable count, yeasts and molds, lactic acid bacteria and Pseudomonads spp. in 70 k V treated samples on day 15 were 3.21, 2.41, 2.44 and 1.96 lg(CFU/g), respectively. The results indicate that DBD-CP treatment could extend the shelf life of braised chicken without negative impacts on quality characteristics.
文摘Aim: To confirm the stability of exogenous genes in the generation of transgenic chickens using ejaculated chicken sperm, the deoxyribonuclease (DNase) activity was evaluated in the seminal plasma of ejaculated semen and the stability of DNA was examined by adding lipofection reagents. Methods: A PCR fragment (249 bp) of pEGFPN-1 vector was used as the DNA substrate and was incubated with the seminal plasma at 40 ℃ for 30 min. Then, the whole reaction solution was subjected to agarose gel electrophoresis and the DNA size was evaluated under UV light. Results: The DNA substrate was completely diminished after incubation with seminal plasma. However, the substrate was intact after incubation with heat-treated seminal plasma or incubation with seminal plasma in the presence of 0.5 mmol/L -5 mmol/L EDTA. The substrate was stabilized in the seminal plasma by the addition of commercially available lipofection reagents. Conclusion: The DNase activity is present in the seminal plasma of ejaculated chicken semen. However, DNA is stable in the liposomal-DNA complex.
文摘The chickens used in this study were from a pure line of brown shell egg-type (YAFA) female grandparents of 17-week-old. Plasma very low density lipoprotein (VLDL) concentration was measured with turbidimetric assay. The experimental results showed that there was a close relationship between plasma VLDL concentration and egg composition traits, and a positive phenotypic correlation trend was found between 29-week plasma VLDL concentration and 44-week yolk weight, and the genetic correlation coefficient was 0.234. Both the phenotypic and genetic correlations between 29-week plasma VLDL concentration and albumen weight were negative. Significant positive phenotypie correlations were, however, observed between yolk ratio, or the yolk/albumen ratio and 29-week plasma VLDL concentration, and their genetic correlation coefficients were 0.250 and 0.223, respectively.
文摘The chickens studied were from a pure line of brown shell egg-type (YAFA) female grandparents of 17-week-old. Plasima very low density lipoprotein (VLDL) concentration was measured with turbidimetric assay. The experiment results showed that the phenotypic correlations between 29-week or 50-week plasma VLDL concentration and egg production (EP) were positive in the early stage of laying period, but those in the latter stage were negative. Selection for low plasma VLDL concentration will decrease the EP in the early stage of laying period but increase the one in the latter stage. There was a significant negative phenotypic correlation between the age at first egg (AFE) and 18-week body weight (BW). The phenotypic correlation between 29-week plasma VLDL concentration and the AFE was negative. AFE was influenced by BW and body fatness as well. There was significant positive phenotypic correlation between plasima VLDL concentration and body weights (BWs) at the same stage in laying period and the phenotypic correlation was also positive between 29-week plasma VLDL concentration and the BW at middle stage of laying period, indicating that selection for low plasma VLDL concentration would reduce BWs at various stages to different degree.
文摘Blood samples were taken from birds,from a commercial line of broiler chicken,being in a fully fed state and in fasting state for 2,4,6 hours respectively at 49 d of age and plasma very low density lipoprotein(VLDL) concentration was measured with turbidimetric assay.The experiment results showed that the variation coefficients of plasma VLDL concentration mersured at four different times were 58.11%,74.39%,69.70%,47.83%,respectively for male broilers,and 41.82%,60.87%.59.26%,50.0%for female broilers respectively.Plasma VLDL concentration decreased with the fasting time.There was a significant positive phenotypic correlation between abdominal fat weight,percentage of abdominal fat and plasma VLDL concentration for the birds being in fully feeding state,and the correlation decreased or no longer existed for the bired being in fasting state.The conclusion was that for practically application of plasma VLDL content selected in broiler chicken breeding,blood samples should be collected from birds being in feeding state.
文摘Two kinds of TiO2-ZnO nano-compounded powders aggregated by spray-drying process and evaporation method were used to deposit photocatalytic coatings by atmospheric plasma spraying technique. The phase compositions, morphologies of the agglomerated TiO2-ZnO powders and the as-sprayed coating were characterized using X-ray diffraction (XRD) and field emission scanning electron microscope (FESEM) respectively. Furthermore, roughness measurements were carried out on their surfaces of the plasma sprayed TiO2-ZnO nano-comPounded coatings. Compared with the TiO2ZnO nano-compounded coating deposited from the spray-dried powder, it was found that the TiO2-ZnO nano-compounded coating deposited from the evaporated powder possesses higher anatase phase. It is ascribed to the existing of partially melted or non-melted microstructure in the TiO2-ZnO coating deposited from the evaporated powder. The partially-meltedor non-melted microstructure was retained from the starting agglomerated powder. This microstructure is beneficial to improve the photocatalytic properties of plasma sprayed TiO2-ZnO nano-compounded coatings.
