[ Objective] This study aimed to investigate the content and antioxidant activity of natural peptides in various soybean sprouts during the germination process. [Method] By using black soybean sprouts, yellow soybean ...[ Objective] This study aimed to investigate the content and antioxidant activity of natural peptides in various soybean sprouts during the germination process. [Method] By using black soybean sprouts, yellow soybean sprouts and green soybean sprouts as raw materials, the content, DPPH ·clearance rate, OH · clearance rate and reduction capacity of natural peptides in various soybean sprouts at different germination time were determined, to evaluate the antioxidant capacity of natural peptides and compare it with that of Vc. [ Result] The results showed that the contents of natural peptides in black soybean sprouts, yellow soybean sprouts and green soybean sprouts varied at different germination time. To be specific, the content of natural peptides in black soybean sprouts reached the maximmn of 0. 194 mg/g at 2 d post-germination; the content of natural pepfides in yellow soybean sprouts reached the maximum of 0.231 mg/g at 2 d post-germination; the content of natural peptides in green soybean sprouts exhibited a negative correlation with the germination time, which reached the maximum of 0. 164 mg/g at 1 d post-germination. [ Conclusion] According to the determination of DPPH ·clearance rate, OH ·clearance rate and reduction capacity of natural peptides in three varieties of soybean sprouts, natural peptides in yellow soybean sprouts possess the highest antioxidant activity, followed by those in black soybean展开更多
以黑暗培养(D)为对照,阐明了蓝光(B)连续光照对大豆(Glycine max L.Merr.)芽苗菜品种东农690生长和类黄酮合成的影响。结果表明:与对照组相比,蓝光连续光照36 h显著提高了大豆芽苗菜子叶中山奈酚(kaempferol)和黄豆苷元(daidzein)的含量...以黑暗培养(D)为对照,阐明了蓝光(B)连续光照对大豆(Glycine max L.Merr.)芽苗菜品种东农690生长和类黄酮合成的影响。结果表明:与对照组相比,蓝光连续光照36 h显著提高了大豆芽苗菜子叶中山奈酚(kaempferol)和黄豆苷元(daidzein)的含量,同时子叶中蓝光光受体基因CRY1、CRY2,类黄酮合成相关基因IFS的表达量也显著提高。处理组中,大豆芽苗菜下胚轴中黄豆苷(daidzin)含量逐渐增加,黄豆苷元(daidzein)、染料木素(genistein)、染料木苷(genistin)和山奈酚(kaempferol)的含量先升高后下降,但在36 h时均显著高于黑暗处理。在蓝光光照6和24 h时,大豆芽苗菜下胚轴中苯丙氨酸解氨酶(PAL)与查尔酮异构酶(CHI)活性与对照组相比均显著提高。类黄酮合成途径相关基因PAL、CHS、ANS、IFS的表达量在蓝光处理下均是先升高后下降,而蓝光光受体基因CRY1与CRY2的表达量随着光照时间延长逐渐升高。展开更多
文摘[ Objective] This study aimed to investigate the content and antioxidant activity of natural peptides in various soybean sprouts during the germination process. [Method] By using black soybean sprouts, yellow soybean sprouts and green soybean sprouts as raw materials, the content, DPPH ·clearance rate, OH · clearance rate and reduction capacity of natural peptides in various soybean sprouts at different germination time were determined, to evaluate the antioxidant capacity of natural peptides and compare it with that of Vc. [ Result] The results showed that the contents of natural peptides in black soybean sprouts, yellow soybean sprouts and green soybean sprouts varied at different germination time. To be specific, the content of natural peptides in black soybean sprouts reached the maximmn of 0. 194 mg/g at 2 d post-germination; the content of natural pepfides in yellow soybean sprouts reached the maximum of 0.231 mg/g at 2 d post-germination; the content of natural peptides in green soybean sprouts exhibited a negative correlation with the germination time, which reached the maximum of 0. 164 mg/g at 1 d post-germination. [ Conclusion] According to the determination of DPPH ·clearance rate, OH ·clearance rate and reduction capacity of natural peptides in three varieties of soybean sprouts, natural peptides in yellow soybean sprouts possess the highest antioxidant activity, followed by those in black soybean
