We consider the conserved charge of static black holes with squashed horizons in the Einstein-Maxwell-dilaton theory via both the Abbott-Deser-Tekin (ADT) method and its off-shell generalization. We first make use o...We consider the conserved charge of static black holes with squashed horizons in the Einstein-Maxwell-dilaton theory via both the Abbott-Deser-Tekin (ADT) method and its off-shell generalization. We first make use of the original ADT method to compute the mass of the dilaton squashed black holes in terms of three different reference spacetimes, which are the asymptotic geometry, the flat background and the spacetime of the Kaluza- Klein monopole with boundary matched to the original metric, respectively. Each mass satisfies the first law of black hole thermodynamics, although the mass computed on the basis of the boundary matching the Kaluza- Klein monopole is different from that of the other two reference spacetimes. Then the mass of the black holes is evaluated through the off-shell generalized ADT method.展开更多
This paper is devoted to the investigation the fermion tunneling radiation of squashed black holes in the G6del universe and charged Kaluza-Klein space-time. For black holes with different dimensions, establishing a s...This paper is devoted to the investigation the fermion tunneling radiation of squashed black holes in the G6del universe and charged Kaluza-Klein space-time. For black holes with different dimensions, establishing a set of appropriate matrices γμ for the general covariant Dirac equation plays an important role in the semi-classical tunneling method. By constructing two sets of γμ matrices, we have successfully derived the tunneling probability and Hawking temperature of the black holes.展开更多
Research shows that producing fermented camel milk is hard because of the milk’s inability to form a firm coagulum, attributed to low levels of κ-casein and ꞵ-lactoglobulin and the large casein micelle size, leading...Research shows that producing fermented camel milk is hard because of the milk’s inability to form a firm coagulum, attributed to low levels of κ-casein and ꞵ-lactoglobulin and the large casein micelle size, leading to a weak network of casein formation. In an effort to address this issue, researchers turned to corn starch as a thickening agent, discovering that a concentration of 2.0% effectively improved the viscosity and significantly reduced syneresis in stirred camel milk yoghurt and cultured camel milk. This study explores alternatives to corn starch, focusing on butternut squash seeds as a promising substitute due to their hydrocolloid composition. By incorporating butternut squash (Cucurbita moschata) seed powder (BSSP) as a thickening agent, this study aimed at enhancing the chemical and rheological properties of stirred camel milk yoghurt and cultured camel milk. Fermented camel milk was prepared using 4 litres of camel milk, 2% starter cultures (thermophilic culture for yoghurt and mesophilic aromatic culture for stirred cultured camel milk) and BSSP 0.0% (negative control), 0.4%, 0.8%, 1.2%, 1.6%, 2.0% mixed with 0.4% gelatin. 2.0% corn starch mixed with 0.4% gelatin was used as a standard for comparison. Results showed that increasing the BSSP level significantly (p < 0.05) decreased the moisture content while increasing the total solid content of stirred fermented camel milk products. There was an increase in ash content with an increase in BSSP levels. There was a significant (p < 0.05) reduction in the pH, with an increase in BSSP levels in stirred fermented camel milk samples. Increasing the concentration of BSSP from 0.4% to 2.0% resulted in a significant (p < 0.05) increase in viscosity and a reduction in syneresis of stirred camel milk yoghurt and stirred cultured camel milk samples. This study demonstrated that BSSP effectively enhances the viscosity, reduces syneresis and increases acidity in stirred fermented camel milk products during storage.展开更多
Acorn squash(Cucurbita pepo)is an iconic fall vegetable in the United States,known for its unique fruit shape and also prized for its culinary properties.Little is known about the metabolism that underlies the develop...Acorn squash(Cucurbita pepo)is an iconic fall vegetable in the United States,known for its unique fruit shape and also prized for its culinary properties.Little is known about the metabolism that underlies the development of fruit quality attributes such as color,sweetness,texture and nutritional qualities in acorn squash,or any other winter squash grown worldwide.To provide insight into winter squash fruit and seed development and add to the genomic resources in the Cucurbita genus,RNA sequencing was used to generate an acorn squash fruit and seed transcriptome from the cultivar Sweet REBA at critical points throughout fruit development.141838600 high-quality paired-end Illumina reads were assembled into 55949 unigenes.85%of unigenes with predicted open reading frames had homology with previously identified genes and over 62%could be functionally annotated.Comparison with the watermelon and cucumber genomes provided confirmation that the unigenes are full-length and comprehensive,covering an average of 90%of the coding sequence of their homologs and 72%of the cucumber and watermelon exomes.Key candidate genes associated with carotenoid and carbohydrate metabolism were identified toward a resource for winter squash fruit quality trait dissection.This transcriptome represents a major advance in C.pepo genomics,providing significant new sequence information and revealing the repertoire of genes expressed throughout winter squash fruit and seed development.Future studies on the genetic basis of fruit quality and future breeding efforts will be enhanced by tools and insights developed from this resource.展开更多
Both the fruit mesocarp and the seeds of winter squash can be used for consumption,although the focus of breeding efforts varies by cultivar.Cultivars bred for fruit consumption are selected for fruit mesocarp quality...Both the fruit mesocarp and the seeds of winter squash can be used for consumption,although the focus of breeding efforts varies by cultivar.Cultivars bred for fruit consumption are selected for fruit mesocarp quality traits such as carotenoid content,percent dry matter,and percent soluble solids,while these traits are essentially ignored in oilseed pumpkins.To compare fruit development in these two types of squash,we sequenced the fruit transcriptome of two cultivars bred for different purposes:an acorn squash,‘Sweet REBA’,and an oilseed pumpkin,‘Lady Godiva’.Putative metabolic pathways were developed for carotenoid,starch,and sucrose synthesis in winter squash fruit and squash homologs were identified for each of the structural genes in the pathways.Gene expression,especially of known rate-limiting and branch point genes,corresponded with metabolite accumulation both across development and between the two cultivars.Thus,developmental regulation of metabolite genes is an important factor in winter squash fruit quality.展开更多
Fruit yield, yield components, fruit mineral content, total phenolic content, antioxidant activity and irrigation water use efifciency (IWUE) of summer squash responses to different irrigation quantities were evalua...Fruit yield, yield components, fruit mineral content, total phenolic content, antioxidant activity and irrigation water use efifciency (IWUE) of summer squash responses to different irrigation quantities were evaluated with a ifeld study. Irrigations were done when the total evaporated water from a Class A pan was about 30 mm. Different irrigation quantities were adjusted using three different plant-pan coefifcients (Kcp, 100% (Kcp1), 85% (Kcp2) and 70% (Kcp3)). Results indicated that lower irrigation quantities provided statistically lower yield and yield components. The highest seasonal fruit yield (80.0 t ha-1) was determined in the Kcp1 treatment, which applied the highest volume of irrigation water (452.9 mm). The highest early fruit yield, average fruit weight and fruit diameter, length and number per plant were also determined in the Kcp1 treatment, with values of 7.25 t ha-1, 264.1 g, 5.49 cm, 19.95 cm and 10.92, respectively. Although the IWUE value was the highest in the Kcp1 treatment (176.6 kg ha-1 mm-1), it was statistically similar to the value for Kcp3 treatment (157.1 kg ha-1 mm-1). Total phenolic content and antioxidant activity of fruits was higher in the Kcp1 (44.27 μg gallic acid equivalents (GAE) mg-1 fresh sample) and in the Kcp2 (84.75%) treatments, respectively. Major (Na, N, P, K, Ca, Mg and S) and trace (Fe, Cu, Mn, Zn and B) mineral contents of squash fruits were the highest in the Kcp2 treatment, with the exception of P, Ca and Cu. Mineral contents and total phenolic content were signiifcantly affected by irrigation quantities, but antioxidant activity was not affected. It can be concluded that the Kcp1 treatment was the most suitable for achieving higher yield and IWUE. However, the Kcp2 treatment will be the most suitable due to the high fruit quality and relatively high yield in water shortage conditions.展开更多
It has been reported that squash leaf curl China virus(SLCCNV)infects some Cucurbitaceae crops except for melon(Cucumis melo L.).A new disease of melon exhibiting severe leaf curl and dwarfing was observed in Hainan P...It has been reported that squash leaf curl China virus(SLCCNV)infects some Cucurbitaceae crops except for melon(Cucumis melo L.).A new disease of melon exhibiting severe leaf curl and dwarfing was observed in Hainan Province of China.In this study,the pathogen was identified as SLCCNV through biological and molecular characterization.The isolate(SLCCNV-HN)possess a bipartite genome,DNA-A(HM566112.1)with the highest nucleotide identity(99%)to SLCCNV-Hn(MF062251.1)pumpkin and SLCCNV-Hn61(AM260205.1)squash isolates from China,whereas DNA-B(HM566113.1)with the highest nucleotide identity(99%)to SLCCNV-Hn(MF062252.1).Phylogenetic analyses based on the full-length SLCCNV-HN DNA-A and-B sequences indicated that SLCCNV-HN melon isolate is clustered with SLCCNV-Hn pumpkin,SLCCNV-Hn61 and SLCCNV-SY squash isolates from southern China,forming an independent cluster.Infectious clone of SLCCNV-HN was constructed and the melon plants were inoculated and the infection rate is 100%,the systemic symptoms in melon showed identical to those of melon plants infected in fields.Additionally,melon plants transmission of this virus by Bemisia tabaci with a transmission rate of 95%(19/20)showed leaf curl and dwarf symptoms 15 days post transmission,thereby fulfilling Koch’s postulates.Analysis of genomic organization and phylogenetic trees indicated that SLCCNV-HN melon isolate belongs to the Begomovirus genus.To the best of our knowledge,this is the first characterization of meloninfecting SLCCNV through its genome,infectious clone and transmission.展开更多
In this note we show that for a given controllable pair (A, B) and any λ > 0, a gain matrix K can be chosen so that the transition matrix e {(A+BK)t} of the system x = (A + BK) x decays at the exponential rate e ?...In this note we show that for a given controllable pair (A, B) and any λ > 0, a gain matrix K can be chosen so that the transition matrix e {(A+BK)t} of the system x = (A + BK) x decays at the exponential rate e ?λt and the overshoot of the transition matrix can be bounded by Mλ L for some constants M and L that are independent of λ. As a consequence, for any h > 0, a gain matrix K can be chosen so that the magnitude of the transition matrix e (A+BK)t can be reduced by 1/2 (or by any given portion) over [0, h]. An interesting application of the result is in the stabilization of switched linear systems with any given switching rate.展开更多
Previous studies reported that some plants, including butternut squash, exert positive effects on the brain. However, few studies have examined the effects of butternut squash on learning, memory, and neurogenesis. Th...Previous studies reported that some plants, including butternut squash, exert positive effects on the brain. However, few studies have examined the effects of butternut squash on learning, memory, and neurogenesis. This study studied the effects of butternut squash extract on spatial learning and cell proliferation in the dentate gyrus of healthy male rats. Thirty-five male Wistar rats were intraperitoneally injected with 0, 50, 100, 200 and 400 mg/kg butternut squash extract once daily for 2 months. After the last administration, rat's spatial memory was studied using the Morris water maze. Finally, rats were sacrificed and hippocampal sections were prepared for light microscopy and bromodeoxyuridine immunohistochemistry studies. The results revealed that escape latency and swim distance decreased in all treatment groups compared with the control rats, and that the number of bromodeoxyuridine-positive cells in the dentate gyrus was significantly increased in the treatment groups compared with the controls. These findings suggest that butternut squash extract improves the learning and memory abilities of male rats, and increases the proliferation of dentate gyrus cells.展开更多
[Objectives]In 2019,virus diseases occurred widely on zucchini planted in Shandong Province.The disease symptom was different from previous reports.This study aimed to identify the pathogen causing the zucchini virus ...[Objectives]In 2019,virus diseases occurred widely on zucchini planted in Shandong Province.The disease symptom was different from previous reports.This study aimed to identify the pathogen causing the zucchini virus disease.[Methods]Ten diseased zucchini leaves were collected in the field and used as materials for PCR and sequencing.[Results]PCR detection and sequencing showed that the nucleotide sequence of the amplified fragment had the highest identity with the squash isolate of squash leaf curl China virus(SLCCNV)(MW389919.1)in Guangdong Province.Primers were further designed for amplifying the full-length SLCCNV.The full-length DNA-A was 2730 bp(OM692270.1),and the full-length DNA-B was 2711 bp(OM692269.1).Through sequence alignment,it was found that the DNA-A sequence shared identity of 89.65%-99.42%with registered SLCCNV,and the identity with the SLCCNV-GDHY pumpkin isolate(MW389919.1)in Guangdong was the highest,at 99.42%.The DNA-B sequence was identical with registered SLCCNV in the range of 81.82%-97.29%,and the identity with the SLCCNV-GDHY pumpkin isolate(MW389918.1)in Guangdong,was the highest,at 97.29%.Therefore,it was speculated that SLCCNV is the pathogen of zucchini virus disease.Since the virus was first found on zucchini in Shandong,it was named SLCCNV-SD.[Conclusions]This study provides materials for the research on the spread of SLCCNV in China and the analysis of population genetic characteristics,as well as a reference for the prevention and control of the virus in zucchini.展开更多
By treatment with oxalate muskmelon cultivar “Wangwenxiang”, which is sensitive to squash mosaic virus (SqMV), develops systemic resistance to SqMV significantly. The challenge inoculation experiments indicate that ...By treatment with oxalate muskmelon cultivar “Wangwenxiang”, which is sensitive to squash mosaic virus (SqMV), develops systemic resistance to SqMV significantly. The challenge inoculation experiments indicate that the symptom of oxalate treated plants gets much slighter, the virus content is only 4% reduced to. The peroxidase activity increases by five times, three new isoperoxidases are induced, and lignin content increases by 82.9%. These results indicate that oxalate induces systemic resistance of muskmelon to SqMV while it induces increase of peroxidase activity, new isoperoxidases and content of lignin in the plants treated with oxalate.展开更多
By Using ADAM4000 series data acquisition module, squash hall monitoring system was developed based on the LabVIEW platform. The system consists of master control, signal channel, file operations, digital filtering, s...By Using ADAM4000 series data acquisition module, squash hall monitoring system was developed based on the LabVIEW platform. The system consists of master control, signal channel, file operations, digital filtering, spectral analysis, statistic analysis, system monitoring and other modules. The system will alarm at the real time when the result of the average temperature of the squash hall divided by ten is no less than the monitor threshold of 4.3 while athletes are playing squash, by which the temperature and pressure data acquisition, processing and monitoring could be achieved. Application shows that the changes of human exposure temperature between 20°C and 43°C can achieve the comfort level of athletes’ movement, proving that the monitoring system effectively improves the security of squash hall indoor temperature environment.展开更多
Greenhouse summer squash is a major type of greenhouse vegetable in Shandong Province.In summer,there is a three-month idle period of greenhouse summer squash.Volvariella volvacea is a high temperature resistant high-...Greenhouse summer squash is a major type of greenhouse vegetable in Shandong Province.In summer,there is a three-month idle period of greenhouse summer squash.Volvariella volvacea is a high temperature resistant high-quality edible fungus.In practice,it is able to use the spent mushroom substrate of Flammulina velutipes to cultivate V.volvacea.In the idle period of greenhouse summer squash,cultivating V.volvacea using the spent mushroom substrate of F.velutipes has high economic and environmental benefits.The cultivation techniques mainly include the preparation of cultivation materials,preliminary preparation for the greenhouse,planting management of V.volvacea,and returning the spent mushroom substrate to the field.By comparison with the conventional summer squash planting,this technique has higher economic benefits and its application prospects will be broad.展开更多
Winter squash (var. Ajijimang) washed in 100 mg.L-1 chlorine water and cut into 4 and 8 parts was packed in different packaging materials, 35 μm micro-perforated P-Plus (MPP), 50 μm polyethylene (PE), 80 μm nylon/P...Winter squash (var. Ajijimang) washed in 100 mg.L-1 chlorine water and cut into 4 and 8 parts was packed in different packaging materials, 35 μm micro-perforated P-Plus (MPP), 50 μm polyethylene (PE), 80 μm nylon/PE (Ny/PE), and 90 μm PE films separately. Fresh-cut samples were then stored at 10℃ for up to 17 days. The 35 μm MPP having a high oxygen transmission rate (OTR) has the most stable gas exchange ratio while the 80 μm Ny/PE was the most unstable. Firmness of the mesocarp remained acceptable until the end of storage regardless of the cutting size. Soluble solids content (SSC) remained stable and even slightly increased to a certain extent within 6 days. An increase in pH after 10-day storage suggested the activity of microorganisms within the packaging films. Strong off-odor was detected in samples packaged in 80 μm Ny/PE film after 13 days of storage. Mold was observed in all 1/4 cut samples on day 13 except the sample packaged with 80 μm Ny/PE film. However, no mold was found in all 1/8 cut samples except 35 μm MPP film on day 13. Severe mold invasion was apparent on the onset of the 17-day storage in 35 μm MPP film and 50 μm PE film. Samples cut into 8 parts and packed in 80 μm Ny/PE and 90 μm PE films had less mold incidence compared with other two packaging films. Fresh-cut winter squash packaged in 90 μm PE film maintained quality with shelf-life of 10 days at 10℃. Winter squash as fresh-cut commodity, with the right packaging material has the potential for longer period of storage in retail stores.展开更多
Coat protein (CP) gene of the Chinese squash !eaf curl virus (SqLCV-C) was amplified through PC’R, cloned and completely sequenced. Based on the comparisons at the levels of both CP gene nucleotide and CP -deduced am...Coat protein (CP) gene of the Chinese squash !eaf curl virus (SqLCV-C) was amplified through PC’R, cloned and completely sequenced. Based on the comparisons at the levels of both CP gene nucleotide and CP -deduced amino acid sequences with other geminiviruses, SqLCV-C is confirmed to be distinct from the American squash leaf curl virus (SqLCV-E). It is a new geminivirus transmitted by whitefly Bemisia tabaci, which infects dicotyledonous plants and is more closely related to the Indian cassava mosaic virus (ICMV).展开更多
Bitter gourd (Momordica charantia L.) seeds contain several squash-type serine proteinase inhibitors (PIs), which inhibit the digestive proteinases of the polyphagous insect pest Helicoverpa armigera. In the prese...Bitter gourd (Momordica charantia L.) seeds contain several squash-type serine proteinase inhibitors (PIs), which inhibit the digestive proteinases of the polyphagous insect pest Helicoverpa armigera. In the present work isolation of a DNA sequence encoding the mature peptide of a trypsin inhibitor McTI-Ⅱ, its cloning and expression as a recombinant protein using Pichia pastoris have been reported. Recombinant McTI-Ⅱ inhibited bovine trypsin at 1 : 1 molar ratio, as expected, but did not inhibit chymotrypsin or elastase. McTI-Ⅱ also strongly inhibited trypsin-like proteinases (81% inhibition) as well as the total proteolytic activity of digestive proteinases (70% inhibition) from the midgut of H. armigera larvae. The insect larvae fed with McTI-Ⅱ-incorporated artificial diet suffered over 70% reduction in the average larval weight after 12 days of feeding. Moreover, ingestion of McTI-Ⅱ resulted in 23% mortality in the larval population. The strong antimetabolic activity of McTI-Ⅱ toward H. armigera indicates its probable use in developing insect tolerance in susceptible plants.展开更多
Trichosanthes trypsin inhibitor (TTI) is a peptide consisting of 27 amino acid residues with three pairs of disulfide bonds. This paper reports the total synthesis and disulfide bond refolding of this inhibitor and it...Trichosanthes trypsin inhibitor (TTI) is a peptide consisting of 27 amino acid residues with three pairs of disulfide bonds. This paper reports the total synthesis and disulfide bond refolding of this inhibitor and its analogue. After purification, the amino acid sequence and stoichiometrical inhibitory activity against trypsin of the synthetic inhibitor were compatible with those of the natural inhibitor. The analogue of this inhibitor in which residue Met in position 6 was replaced by Ala was also synthesized. The antitrypsin activity of this synthetic analogue was also approximate to that of the natural inhibitor.展开更多
基金Supported by the National Natural Science Foundation of China under Grant Nos 11275157 and 11505036the Doctoral Research Fund of Guizhou Normal University in 2014+1 种基金the Technology Department of Guizhou Province Fund under Grant No[2015]2114the Science and Technology Innovation Talent Team of Guizhou Province under Grant No(2015)4015
文摘We consider the conserved charge of static black holes with squashed horizons in the Einstein-Maxwell-dilaton theory via both the Abbott-Deser-Tekin (ADT) method and its off-shell generalization. We first make use of the original ADT method to compute the mass of the dilaton squashed black holes in terms of three different reference spacetimes, which are the asymptotic geometry, the flat background and the spacetime of the Kaluza- Klein monopole with boundary matched to the original metric, respectively. Each mass satisfies the first law of black hole thermodynamics, although the mass computed on the basis of the boundary matching the Kaluza- Klein monopole is different from that of the other two reference spacetimes. Then the mass of the black holes is evaluated through the off-shell generalized ADT method.
文摘This paper is devoted to the investigation the fermion tunneling radiation of squashed black holes in the G6del universe and charged Kaluza-Klein space-time. For black holes with different dimensions, establishing a set of appropriate matrices γμ for the general covariant Dirac equation plays an important role in the semi-classical tunneling method. By constructing two sets of γμ matrices, we have successfully derived the tunneling probability and Hawking temperature of the black holes.
文摘Research shows that producing fermented camel milk is hard because of the milk’s inability to form a firm coagulum, attributed to low levels of κ-casein and ꞵ-lactoglobulin and the large casein micelle size, leading to a weak network of casein formation. In an effort to address this issue, researchers turned to corn starch as a thickening agent, discovering that a concentration of 2.0% effectively improved the viscosity and significantly reduced syneresis in stirred camel milk yoghurt and cultured camel milk. This study explores alternatives to corn starch, focusing on butternut squash seeds as a promising substitute due to their hydrocolloid composition. By incorporating butternut squash (Cucurbita moschata) seed powder (BSSP) as a thickening agent, this study aimed at enhancing the chemical and rheological properties of stirred camel milk yoghurt and cultured camel milk. Fermented camel milk was prepared using 4 litres of camel milk, 2% starter cultures (thermophilic culture for yoghurt and mesophilic aromatic culture for stirred cultured camel milk) and BSSP 0.0% (negative control), 0.4%, 0.8%, 1.2%, 1.6%, 2.0% mixed with 0.4% gelatin. 2.0% corn starch mixed with 0.4% gelatin was used as a standard for comparison. Results showed that increasing the BSSP level significantly (p < 0.05) decreased the moisture content while increasing the total solid content of stirred fermented camel milk products. There was an increase in ash content with an increase in BSSP levels. There was a significant (p < 0.05) reduction in the pH, with an increase in BSSP levels in stirred fermented camel milk samples. Increasing the concentration of BSSP from 0.4% to 2.0% resulted in a significant (p < 0.05) increase in viscosity and a reduction in syneresis of stirred camel milk yoghurt and stirred cultured camel milk samples. This study demonstrated that BSSP effectively enhances the viscosity, reduces syneresis and increases acidity in stirred fermented camel milk products during storage.
基金This research was supported through funds from Cornell University and the Vegetable Breeding InstituteSupport for Lindsay Wyatt was provided by a Cornell University Presidential Life Sciences Fellowship,USDA National Needs Graduate Fellowship Competitive Grant No.2008-38420-04755 from the National Institute of Food and Agriculturethe Agriculture and Food Research Initiative Competitive Grant No.2013-67011-21122 from the USDA National Institute of Food and Agriculture.
文摘Acorn squash(Cucurbita pepo)is an iconic fall vegetable in the United States,known for its unique fruit shape and also prized for its culinary properties.Little is known about the metabolism that underlies the development of fruit quality attributes such as color,sweetness,texture and nutritional qualities in acorn squash,or any other winter squash grown worldwide.To provide insight into winter squash fruit and seed development and add to the genomic resources in the Cucurbita genus,RNA sequencing was used to generate an acorn squash fruit and seed transcriptome from the cultivar Sweet REBA at critical points throughout fruit development.141838600 high-quality paired-end Illumina reads were assembled into 55949 unigenes.85%of unigenes with predicted open reading frames had homology with previously identified genes and over 62%could be functionally annotated.Comparison with the watermelon and cucumber genomes provided confirmation that the unigenes are full-length and comprehensive,covering an average of 90%of the coding sequence of their homologs and 72%of the cucumber and watermelon exomes.Key candidate genes associated with carotenoid and carbohydrate metabolism were identified toward a resource for winter squash fruit quality trait dissection.This transcriptome represents a major advance in C.pepo genomics,providing significant new sequence information and revealing the repertoire of genes expressed throughout winter squash fruit and seed development.Future studies on the genetic basis of fruit quality and future breeding efforts will be enhanced by tools and insights developed from this resource.
基金This research was supported through startup funds from Cornell UniversitySupport for Lindsay Wyatt was provided by a Cornell University Presidential Life Sciences Fellowship,USDA National Needs Graduate Fellowship Competitive Grant No.2008-38420-04755 from the National Institute of Food and Agriculturethe Agriculture and Food Research Initiative Competitive Grant No.2013-67011-21122 from the USDA National Institute of Food and Agriculture.
文摘Both the fruit mesocarp and the seeds of winter squash can be used for consumption,although the focus of breeding efforts varies by cultivar.Cultivars bred for fruit consumption are selected for fruit mesocarp quality traits such as carotenoid content,percent dry matter,and percent soluble solids,while these traits are essentially ignored in oilseed pumpkins.To compare fruit development in these two types of squash,we sequenced the fruit transcriptome of two cultivars bred for different purposes:an acorn squash,‘Sweet REBA’,and an oilseed pumpkin,‘Lady Godiva’.Putative metabolic pathways were developed for carotenoid,starch,and sucrose synthesis in winter squash fruit and squash homologs were identified for each of the structural genes in the pathways.Gene expression,especially of known rate-limiting and branch point genes,corresponded with metabolite accumulation both across development and between the two cultivars.Thus,developmental regulation of metabolite genes is an important factor in winter squash fruit quality.
文摘Fruit yield, yield components, fruit mineral content, total phenolic content, antioxidant activity and irrigation water use efifciency (IWUE) of summer squash responses to different irrigation quantities were evaluated with a ifeld study. Irrigations were done when the total evaporated water from a Class A pan was about 30 mm. Different irrigation quantities were adjusted using three different plant-pan coefifcients (Kcp, 100% (Kcp1), 85% (Kcp2) and 70% (Kcp3)). Results indicated that lower irrigation quantities provided statistically lower yield and yield components. The highest seasonal fruit yield (80.0 t ha-1) was determined in the Kcp1 treatment, which applied the highest volume of irrigation water (452.9 mm). The highest early fruit yield, average fruit weight and fruit diameter, length and number per plant were also determined in the Kcp1 treatment, with values of 7.25 t ha-1, 264.1 g, 5.49 cm, 19.95 cm and 10.92, respectively. Although the IWUE value was the highest in the Kcp1 treatment (176.6 kg ha-1 mm-1), it was statistically similar to the value for Kcp3 treatment (157.1 kg ha-1 mm-1). Total phenolic content and antioxidant activity of fruits was higher in the Kcp1 (44.27 μg gallic acid equivalents (GAE) mg-1 fresh sample) and in the Kcp2 (84.75%) treatments, respectively. Major (Na, N, P, K, Ca, Mg and S) and trace (Fe, Cu, Mn, Zn and B) mineral contents of squash fruits were the highest in the Kcp2 treatment, with the exception of P, Ca and Cu. Mineral contents and total phenolic content were signiifcantly affected by irrigation quantities, but antioxidant activity was not affected. It can be concluded that the Kcp1 treatment was the most suitable for achieving higher yield and IWUE. However, the Kcp2 treatment will be the most suitable due to the high fruit quality and relatively high yield in water shortage conditions.
基金supported by the National Natural Science Foundation of China (31701941 and 31401810)the grants from the earmarked fund for China Agriculture Research System (CARS-26-13)the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences (ASTIP) (CAAS-ASTIP-2018-ZFRI-08)
文摘It has been reported that squash leaf curl China virus(SLCCNV)infects some Cucurbitaceae crops except for melon(Cucumis melo L.).A new disease of melon exhibiting severe leaf curl and dwarfing was observed in Hainan Province of China.In this study,the pathogen was identified as SLCCNV through biological and molecular characterization.The isolate(SLCCNV-HN)possess a bipartite genome,DNA-A(HM566112.1)with the highest nucleotide identity(99%)to SLCCNV-Hn(MF062251.1)pumpkin and SLCCNV-Hn61(AM260205.1)squash isolates from China,whereas DNA-B(HM566113.1)with the highest nucleotide identity(99%)to SLCCNV-Hn(MF062252.1).Phylogenetic analyses based on the full-length SLCCNV-HN DNA-A and-B sequences indicated that SLCCNV-HN melon isolate is clustered with SLCCNV-Hn pumpkin,SLCCNV-Hn61 and SLCCNV-SY squash isolates from southern China,forming an independent cluster.Infectious clone of SLCCNV-HN was constructed and the melon plants were inoculated and the infection rate is 100%,the systemic symptoms in melon showed identical to those of melon plants infected in fields.Additionally,melon plants transmission of this virus by Bemisia tabaci with a transmission rate of 95%(19/20)showed leaf curl and dwarf symptoms 15 days post transmission,thereby fulfilling Koch’s postulates.Analysis of genomic organization and phylogenetic trees indicated that SLCCNV-HN melon isolate belongs to the Begomovirus genus.To the best of our knowledge,this is the first characterization of meloninfecting SLCCNV through its genome,infectious clone and transmission.
基金This work was supported partly by the Chinese National Natural Science Foundation. The work of Wang was also supported partly by the US National Science Foundation (No. DMS - 0072620).
文摘In this note we show that for a given controllable pair (A, B) and any λ > 0, a gain matrix K can be chosen so that the transition matrix e {(A+BK)t} of the system x = (A + BK) x decays at the exponential rate e ?λt and the overshoot of the transition matrix can be bounded by Mλ L for some constants M and L that are independent of λ. As a consequence, for any h > 0, a gain matrix K can be chosen so that the magnitude of the transition matrix e (A+BK)t can be reduced by 1/2 (or by any given portion) over [0, h]. An interesting application of the result is in the stabilization of switched linear systems with any given switching rate.
基金a grant from the Research Institute for Islamic and Complementary Medicine (RICM),Tehran University of Medical Sciences,No. p26/m/t/1088
文摘Previous studies reported that some plants, including butternut squash, exert positive effects on the brain. However, few studies have examined the effects of butternut squash on learning, memory, and neurogenesis. This study studied the effects of butternut squash extract on spatial learning and cell proliferation in the dentate gyrus of healthy male rats. Thirty-five male Wistar rats were intraperitoneally injected with 0, 50, 100, 200 and 400 mg/kg butternut squash extract once daily for 2 months. After the last administration, rat's spatial memory was studied using the Morris water maze. Finally, rats were sacrificed and hippocampal sections were prepared for light microscopy and bromodeoxyuridine immunohistochemistry studies. The results revealed that escape latency and swim distance decreased in all treatment groups compared with the control rats, and that the number of bromodeoxyuridine-positive cells in the dentate gyrus was significantly increased in the treatment groups compared with the controls. These findings suggest that butternut squash extract improves the learning and memory abilities of male rats, and increases the proliferation of dentate gyrus cells.
基金Supported by Shandong Province Key R&D Program(2021LZGC015)Taishan Industry Leading Talent Project(LJNY201812)。
文摘[Objectives]In 2019,virus diseases occurred widely on zucchini planted in Shandong Province.The disease symptom was different from previous reports.This study aimed to identify the pathogen causing the zucchini virus disease.[Methods]Ten diseased zucchini leaves were collected in the field and used as materials for PCR and sequencing.[Results]PCR detection and sequencing showed that the nucleotide sequence of the amplified fragment had the highest identity with the squash isolate of squash leaf curl China virus(SLCCNV)(MW389919.1)in Guangdong Province.Primers were further designed for amplifying the full-length SLCCNV.The full-length DNA-A was 2730 bp(OM692270.1),and the full-length DNA-B was 2711 bp(OM692269.1).Through sequence alignment,it was found that the DNA-A sequence shared identity of 89.65%-99.42%with registered SLCCNV,and the identity with the SLCCNV-GDHY pumpkin isolate(MW389919.1)in Guangdong was the highest,at 99.42%.The DNA-B sequence was identical with registered SLCCNV in the range of 81.82%-97.29%,and the identity with the SLCCNV-GDHY pumpkin isolate(MW389918.1)in Guangdong,was the highest,at 97.29%.Therefore,it was speculated that SLCCNV is the pathogen of zucchini virus disease.Since the virus was first found on zucchini in Shandong,it was named SLCCNV-SD.[Conclusions]This study provides materials for the research on the spread of SLCCNV in China and the analysis of population genetic characteristics,as well as a reference for the prevention and control of the virus in zucchini.
文摘By treatment with oxalate muskmelon cultivar “Wangwenxiang”, which is sensitive to squash mosaic virus (SqMV), develops systemic resistance to SqMV significantly. The challenge inoculation experiments indicate that the symptom of oxalate treated plants gets much slighter, the virus content is only 4% reduced to. The peroxidase activity increases by five times, three new isoperoxidases are induced, and lignin content increases by 82.9%. These results indicate that oxalate induces systemic resistance of muskmelon to SqMV while it induces increase of peroxidase activity, new isoperoxidases and content of lignin in the plants treated with oxalate.
文摘By Using ADAM4000 series data acquisition module, squash hall monitoring system was developed based on the LabVIEW platform. The system consists of master control, signal channel, file operations, digital filtering, spectral analysis, statistic analysis, system monitoring and other modules. The system will alarm at the real time when the result of the average temperature of the squash hall divided by ten is no less than the monitor threshold of 4.3 while athletes are playing squash, by which the temperature and pressure data acquisition, processing and monitoring could be achieved. Application shows that the changes of human exposure temperature between 20°C and 43°C can achieve the comfort level of athletes’ movement, proving that the monitoring system effectively improves the security of squash hall indoor temperature environment.
基金Sub-topics of National Key R&D program(2016YFD201306-03)Science and Technology Innovation Project of Shandong Academy of Agricultural Sciences(CXGC2016B09).
文摘Greenhouse summer squash is a major type of greenhouse vegetable in Shandong Province.In summer,there is a three-month idle period of greenhouse summer squash.Volvariella volvacea is a high temperature resistant high-quality edible fungus.In practice,it is able to use the spent mushroom substrate of Flammulina velutipes to cultivate V.volvacea.In the idle period of greenhouse summer squash,cultivating V.volvacea using the spent mushroom substrate of F.velutipes has high economic and environmental benefits.The cultivation techniques mainly include the preparation of cultivation materials,preliminary preparation for the greenhouse,planting management of V.volvacea,and returning the spent mushroom substrate to the field.By comparison with the conventional summer squash planting,this technique has higher economic benefits and its application prospects will be broad.
文摘Winter squash (var. Ajijimang) washed in 100 mg.L-1 chlorine water and cut into 4 and 8 parts was packed in different packaging materials, 35 μm micro-perforated P-Plus (MPP), 50 μm polyethylene (PE), 80 μm nylon/PE (Ny/PE), and 90 μm PE films separately. Fresh-cut samples were then stored at 10℃ for up to 17 days. The 35 μm MPP having a high oxygen transmission rate (OTR) has the most stable gas exchange ratio while the 80 μm Ny/PE was the most unstable. Firmness of the mesocarp remained acceptable until the end of storage regardless of the cutting size. Soluble solids content (SSC) remained stable and even slightly increased to a certain extent within 6 days. An increase in pH after 10-day storage suggested the activity of microorganisms within the packaging films. Strong off-odor was detected in samples packaged in 80 μm Ny/PE film after 13 days of storage. Mold was observed in all 1/4 cut samples on day 13 except the sample packaged with 80 μm Ny/PE film. However, no mold was found in all 1/8 cut samples except 35 μm MPP film on day 13. Severe mold invasion was apparent on the onset of the 17-day storage in 35 μm MPP film and 50 μm PE film. Samples cut into 8 parts and packed in 80 μm Ny/PE and 90 μm PE films had less mold incidence compared with other two packaging films. Fresh-cut winter squash packaged in 90 μm PE film maintained quality with shelf-life of 10 days at 10℃. Winter squash as fresh-cut commodity, with the right packaging material has the potential for longer period of storage in retail stores.
文摘Coat protein (CP) gene of the Chinese squash !eaf curl virus (SqLCV-C) was amplified through PC’R, cloned and completely sequenced. Based on the comparisons at the levels of both CP gene nucleotide and CP -deduced amino acid sequences with other geminiviruses, SqLCV-C is confirmed to be distinct from the American squash leaf curl virus (SqLCV-E). It is a new geminivirus transmitted by whitefly Bemisia tabaci, which infects dicotyledonous plants and is more closely related to the Indian cassava mosaic virus (ICMV).
文摘Bitter gourd (Momordica charantia L.) seeds contain several squash-type serine proteinase inhibitors (PIs), which inhibit the digestive proteinases of the polyphagous insect pest Helicoverpa armigera. In the present work isolation of a DNA sequence encoding the mature peptide of a trypsin inhibitor McTI-Ⅱ, its cloning and expression as a recombinant protein using Pichia pastoris have been reported. Recombinant McTI-Ⅱ inhibited bovine trypsin at 1 : 1 molar ratio, as expected, but did not inhibit chymotrypsin or elastase. McTI-Ⅱ also strongly inhibited trypsin-like proteinases (81% inhibition) as well as the total proteolytic activity of digestive proteinases (70% inhibition) from the midgut of H. armigera larvae. The insect larvae fed with McTI-Ⅱ-incorporated artificial diet suffered over 70% reduction in the average larval weight after 12 days of feeding. Moreover, ingestion of McTI-Ⅱ resulted in 23% mortality in the larval population. The strong antimetabolic activity of McTI-Ⅱ toward H. armigera indicates its probable use in developing insect tolerance in susceptible plants.
基金supported by the State Biological High Technology Research Grant.
文摘Trichosanthes trypsin inhibitor (TTI) is a peptide consisting of 27 amino acid residues with three pairs of disulfide bonds. This paper reports the total synthesis and disulfide bond refolding of this inhibitor and its analogue. After purification, the amino acid sequence and stoichiometrical inhibitory activity against trypsin of the synthetic inhibitor were compatible with those of the natural inhibitor. The analogue of this inhibitor in which residue Met in position 6 was replaced by Ala was also synthesized. The antitrypsin activity of this synthetic analogue was also approximate to that of the natural inhibitor.