Effects of thinning and understory removal on water use efficiency of Pinus massoniana:evidence from photosynthetic capacity and stable carbon isotope analyses

Understanding the relationship between forest management and water use efficiency(WUE)is important for evaluating forest adaptability to climate change.However,the effects of thinning and understory removal on WUE and its key controlling processes are not well understood,which limits our comprehension of the physiological mechanisms of various management practices.In this study,four forest management measures(no thinning:NT;understory removal:UR;light thinning:LT;and heavy thinning:HT)were carried out in Pinus massoniana plantations in a subtropical region of China.Photosynthetic capacity and needle stable carbon isotope composition(δ^(13)C)were measured to assess instantaneous water use efficiency(WUE_(inst))and long-term water use efficiency(WUE_(i)).Multiple regression models and structural equation modelling(SEM)identified the effects of soil properties and physiological performances on WUE_(inst)and WUE_(i).The results show that WUE_(inst)values among the four treatments were insignificant.However,compared with the NT stand(35.8μmol·mol^(-1)),WUE_(i)values significantly increased to 41.7μmol·mol^(-1)in the UR,50.1μmol·mol^(-1)in the LT and 46.6μmol·mol^(-1)in HT treatments,largely explained by photosynthetic capacity and soil water content.Understory removal did not change physiological performance(needle water potential and photosynthetic capacity).Thinning increased the net photosynthetic rate(A_n)but not stomatal conductance(g_s)or predawn needle water potential(ψ_(pd)),implying that the improvement in water use efficiency for thinned stands was largely driven by radiation interception than by soil water availability.In general,thinning may be an appropriate management measure to promote P.massoniana WUE to cope with seasonal droughts under future extreme climates.

Determination of Atomic Fractions of Isotopes Carbon-13 and Nitrogen-15 Directly in Glicine, L-Leucine, Isoleucine and Alanine

Using compounds modified by the isotopes carbon-13 and nitrogen-15 helps conduct research in various fields of science, such as medicine, pharmacology, pharmacokinetics, metabolism, agriculture, and others. In the case of the availability of reliable, express, and cheap methods, the area of their use will gradually expand. A determination of the atomic fraction of the isotopes carbon-13 and nitrogen-15 directly in glycine, leucine, isoleucine, and alanine is proposed;the modification concerns all centers or one or more identical carbon and nitrogen centers separately, as well as both isotopes at the same time. There are defined mass lines of the mass spectrum of each amino acid, through which the isotopic content of carbon and nitrogen is calculated. The processes that must be taken into account for the determination of the isotopic content are also established. Isotopic analysis of these compounds until now was carried out by transforming them into carbon oxide, dioxide, and molecular nitrogen, and determination of their content in individual centers was impossible.

Application of Stable Isotope Techniques in Studies of Carbon and Nitrogen Biogeochemical Cycles of Ecosystem

Stable isotope techniques have been proved useful as tools for studying the carbon (C) and nitrogen (N) biogeochemical cycles of ecosystem. This paper firstly introduced the basic principles and the distribution characteristics of stable isotope, then reviewed the recent advances and applications of stable isotope in the C and N biogeochemical cycles of ecosystem. By applying the 13 C natural abundance technique, ecologists are able to understand the photosynthetic path and CO 2 fixation of plants, the CO 2 exchange and C balance status of ecosystem, the composition, distribution and turnover of soil organic C and the sources of organic matter in food webs, while by using the 13 C labeled technique, the effects of elevated CO 2 on the C processes of ecosystem and the sources and fate of organic matter in ecosystem can be revealed in detail. Differently, by applying the 15 N natural abundance technique, ecologists are able to analyze the biological N 2 -fixation, the N sources of ecosystem, the N transformation processes of ecosystem and the N trophic status in food webs, while by using the 15 N labeled technique, the sources, transformation and fate of N in ecosystem and the effects of N input on the ecosystem can be investigated in depth. The applications of both C and N isotope natural abundance and labeled techniques, combined with the elemental, other isotope ( 34 S) and molecular biomarker information, will be more propitious to the investigation of C and N cycle mechanisms. Finally, this paper concluded the problems existed in current researches, and put forward the perspective of stable isotope techniques in the studies on C and N biogeochemical cycles of ecosystem in the future.

Carbon and nitrogen isotopes analysis and sources of organic matter in surface sediments from the Sanggou Bay and its adjacent areas, China

Naturally existing stable carbon and nitrogen isotopes are important in the study of sedimentary organic matter sources. To identify the sources of sedimentary organic matter in Sanggou Bay and its adjacent areas, which is characterized by high-density shellfish and seaweed aquaculture, the grain size, organic carbon （OC）, total nitrogen （TN）, carbon and nitrogen isotopic composition （δ13C andδ15N） of organic matter in the surface sediment were determined. The results showed that, in August, sedimentary OC and TN ranged from 0.17% to 0.76% and 0.04% to 0.14%, respectively. In November, OC and TN ranged from 0.23% to 0.87% and 0.05% to 0.14%, respectively. There was a significant positive correlation between OC and TN （R=0.98, P＆lt;0.0001）, indicating that OC and TN were homologous. In August, theδ13C andδ15N of organic matter varied from -23.06‰ to -21.59‰ and 5.10‰ to 6.31‰, respectively. In November,δ13C andδ15N ranged from -22.87‰ to -21.34‰ and 5.13‰ to 7.31‰, respectively. This study found that the major sources of sedimentary organic matter were marine shellfish biodeposition, seaweed farming, and soil organic matter. Using a three-end-member mixed model, we estimated that the dominant source of sedimentary organic matter was shellfish biodeposition, with an average contribution rate of 65.53% in August and 43.00% in November. Thus, shellfish farming had a significant influence on the coastal carbon cycle.

Stable Carbon Isotope Geochemical and Hydrochemical Features in the System of Carbonate-H_2O-CO_2 and Their Implications—Evidence from Several Typical Karst Areas of China

On the basis of hydrochemical observation and experimental calculation, the features of stable carbon isotope geochemistry in the karst dynamic systems of the Guilin Karst Experimental Site, Huanglong Ravine and Wujiangdu Dam Site are summarized in this study. Furthermore, an attempt has been made to solve several geochemical problems, such as the origin of CO2 in the system, kinetic fractionation of carbon isotopes during calcite deposition, hydrochemistry and formation of tufa, and carbon-14 dating of tufa of hydrothermal origin. The results show that three kinds of karst dynamic system can be distinguished: (1) the shallow system, such as the Guilin Karst Experimental Site, in which soil CO2 provides the an active agent for karst processes; (2) the geothermal system, such as the Huanglong Ravine, in which metamorphic or/ and juvenile CO, is the source of activity for karst; (3) the anthropogenic system, such as the Wujiangdu Dam Site, in which the stable carbon isotope geochemical and hydrochemical features have been greatly affected by human activity.

Stable Isotope Studies of Crop Carbon and Water Relations:A Review

Crop carbon and water relations research is important in the studies of water saving agriculture, breeding program, and energy and material cycles in soil plant atmosphere continuum （SPAC）. The purpose of this paper is to review the current state of knowledge on stable isotopes of carbon, oxygen, and hydrogen in the research of crop carbon and water relations, such as carbon isotope discrimination （△^13C） during carbon fixation process by photosynthesis, application of △^13C in crop water use efficiency （WUE） and breeding programs, oxygen isotope enrichment during leaf water transpiration, CO2 fixation by photosynthesis and release by respiration, application of hydrogen isotope composition （619） and oxygen isotope composition （6180） for determination of water source used by a crop, stable isotope coupling Keeling plot for investigating the carbon and water flux in ecosystem, energy and material cycle in SPAC and correlative integrative models on stable isotope. These aspects contain most of the stable isotope researches on crop carbon and water relations which have been widely explored internationally while less referred in China. Based on the reviewed literatures, some needs for future research are suggested.

Stable Carbon Isotope Variations in Cave Percolation Waters and their Implications in Four Caves of Guizhou, China

Monitoring and sampling of main plants, soil CO2, soil water, bedrock, spring water, drip water and its corresponding speleothem were performed at four cave systems of Guizhou, Southwest China, from April 2003 to May 2004, in order to understand stable carbon isotope ratios variations of dissolved inorganic Carbon （DIC） in cave percolation waters （δ13CDIC） and their implications for paleoclimate. Stable carbon isotopic compositions and ions （Ca, Mg, Sr, SO4, CI etc.） were measured for all samples. The results indicate that there are significant differences among the δ13CDIC values from inter-cave, even inter-drip of intra-cave in the four caves. The δ13CDIC values from the Liangfeng Cave （LFC） is lightest among the four caves, where vegetation type overlying the cave is primary forest dominated by tall trees with lighter average δ13C value （-29.9‰）. And there are remarkable differences in δ13CDIC values of different drip waters in the Qixing Cave （QXC） and Jiangjun Cave （JJC）, up to 6.9‰ and 7.8‰, respectively. Further analyses show that the δ13CDIC values in cave drip waters are not only controlled by vegetation biomass overlying the cave, but also hydro-geochemical processes. Therefore, accurate interpreting of δ13C recorded in speleothems cannot be guaranteed if these effects of the above mentioned factors are not taken into consideration.

Effects of pretreatment procedures on fatty acid composition and stable carbon isotopes in the marine microalga Isochrysis zhanjiangenisis

This study aims to quantify the effects of different pretreatment methods on the stable carbon isotope values of fatty acids in marine microalgae（Isochrysis zhanjiangenisis）.To identify the effects of sample preparation on theδ^（13）C value and the fatty acid composition,we examined eight types of pretreatment methods including：（a） drying the sample followed by direct methyl esterification using HCl-CH3OH;（b） drying the sample followed by direct methyl esterification using H2SO4-CH3OH;（c） drying the sample by ultrasonic extraction and methylesterification using HCl-CH3OH;（d） drying the sample by ultrasonic extraction and methyl-esterification using H2SO4-CH3OH;（e） fresh sample followed by direct methyl-esterification using HCl-CH3OH;（f） fresh sample followed by direct methyl-esterification using H2SO4-CH3OH;（g） fresh sample with ultrasonic extraction followed by methyl-esterification using HCl-CH3OH,and（h） fresh sample with ultrasonic extraction followed by methylesterification using H2SO4-CH3 OH.The results show that the δ^（13）C values from Groups a-e,g and h fluctuated within 0.3‰,and the δ^（13）C values of Group f were approximately 0.7‰ lower than the other seven groups.Therefore,the different sample pretreatment methods used towards the extraction of fatty acids from marine microalgae may result in different results regarding the stable carbon isotope ratios,and if necessary a correction should be applied.

Sources of particulate organic matter in the Chukchi and Siberian shelves: clues from carbon and nitrogen isotopes

The stable isotopic composition(δ13C andδ15N)and carbon/nitrogen ratio(C/N)of particulate organic matter(POM)in the Chukchi and East Siberian shelves from July to September,2016 were measured to evaluate the spatial variability and origin of POM.Theδ13CPOC values were in the range of−29.5‰to−17.5‰with an average of−25.9‰±2.0‰,and theδ15NPN values ranged from 3.9‰to 13.1‰with an average of 8.0‰±1.6‰.The C/N ratios in the East Siberian shelf were generally higher than those in the Chukchi shelf,while theδ13C andδ15N values were just the opposite.Abnormally low C/N ratios(<4),lowδ13CPOC(almost−28‰)and highδ15NPN(>10‰)values were observed in the Wrangel Island polynya,which was attributed to the early bloom of small phytoplankton.The contributions of terrestrial POM,bloom-produced POM and non-bloom marine POM were estimated using a three end-member mixing model.The spatial distribution of terrestrial POM showed a high fraction in the East Siberian shelf and decreased eastward,indicating the influence of Russian rivers.The distribution of non-bloom marine POM showed a high fraction in the Chukchi shelf with the highest fraction occurring in the Bering Strait and decreased westward,suggesting the stimulation of biological production by the Pacific inflow in the Chukchi shelf.The fractions of bloom-produced POM were highest in the winter polynya and gradually decreased toward the periphery.A negative relationship between the bloom-produced POM and the sea ice meltwater inventory was observed,indicating that the net sea ice loss promotes early bloom in the polynya.Given the high fraction of bloom-produced POM,the early bloom of phytoplankton in the polynyas may play an important role on marine production and POM export in the Arctic shelves.

Sedimentary Records of Mangrove Evolution During the Past one Hundred Years Based on Stable Carbon Isotope and Pollen Evidences in Maowei, SW China

Mangroves accumulate sedimentary sequences, where cores can provide historical records of mangrove evolution with past climate change and human activity. The study traced the history of mangrove evolution during the past one hundred years in a mangrove swamp of Maowei Sea, SW China. The sedimentation rates(0.38-0.95 cm yr^(-1)) were calculated on the basis of ln(^(210)Pb_(xs)/Al) and mass depth in the core sediments. Chemical tracers, such as δ^(13)C_(org) and C:N values, were utilized to trace the contribution of mangrove-derived organic matter using a ternary mixing model. Because of potential diagenetic alteration and / or overlap in the isotopic signatures of different components, simultaneous use of mangrove pollen diagrams can help to supplement some of these limitations. Combined with mangrove pollen, mangrove evolution was reconstructed and could be divided into three stages: flourishment(1886-1905 AD), slight degradation(1905-1949 AD) and rapid degradation period(1949-2007 AD), which was consistent with previous reports. The reclamation of mangrove swamps to shrimp ponds was the major reason for rapid degradation of mangrove ecosystems in recent years, rather than climate change in the region.

Dynamics of soil organic carbon following land-use change:insights from stable C-isotope analysis in black soil of Northeast China

Intensive soil tillage is a significant factor in soil organic matter decline in cultivated soils. Both cultivation abandonment and foregoing tillage have been encouraged in the past 30 years to reduce greenhouse gas emissions and soil erosion. However, the dynamic processes of soil organic carbon (SOC) in areas of either continuous cultivation or abandonment remain unclear and inconsistent.Our aims were to assess and model the dynamic processes of SOC under continuous tillage and after cultivation abandonment in the black soil of Northeast China. Soil profiles were collected of cultivated or abandoned land with cultivation history of 0–100 years. An isotope mass balance equation was used to calculate the proportion of SOC derived from corn debris (C_4) and from natural vegetation (C_3) to deduce the dynamic process. Approximately 40% of SOC in the natural surface soil (0–10 cm) was eroded in the first 5 years of cultivation, increasing to about 75% within 40 years, before a slow recovery. C_4 above 30 cm soil depth increased by 4.5%–5% or 0.11–0.12 g·kg^(-1) on average per year under continuous cultivation, while it decreased by approximately 0.34% annually in the surface soil after cultivation abandonment.The increase in the percentage of C_4 was fitted to a linear equation with given intercepts in the upper 30 cm of soil in cultivated land. A significant relationship between the change of C_4 and time was found only in the surface soil after abandonment of cultivation. These results demonstrate the loss and accumulation of corn-derived SOC in surface black soil of Northeast China under continuous tillage or cultivation abandonment.

Effects of Long-Term Winter Planted Green Manure on Distribution and Storage of Organic Carbon and Nitrogen in Water-Stable Aggregates of Reddish Paddy Soil Under a Double-Rice Cropping System

In agricultural systems, maintenance of soil organic matter has long been recognized as a strategy to reduce soil degradation. Manure amendments and green manures are management practices that can increase some nutrient contents and improve soil aggregation. We investigated the effects of 28 yr of winter planted green manure on soil aggregate-size distribution and aggregateassociated carbon（C） and nitrogen（N）. The study was a randomized completed block design with three replicates. The treatments included rice-rice-fallow, rice-rice-rape, rice-rice-Chinese milk vetch and rice-rice-ryegrass. The experiment was established in 1982 on a silty light clayey paddy soil derived from Quaternary red clay（classified as Fe-Accumuli-Stagnic Anthrosols） with continuous early and late rice. In 2009, soil samples were collected（0-15 cm depth） from the field treatment plots and separated into water-stable aggregates of different sizes（i.e., 〉5, 2-5, 1-2, 0.5-1, 0.25-0.5 and 〈0.25 mm） by wet sieving. The long-term winter planted green manure significantly increased total C and N, and the formation of the 2-5-mm water-stable aggregate fraction. Compared with rice-rice-rape, rice-rice-Chinese milk vetch and rice-rice-ryegrass, the rice-rice-fallow significantly reduced 2-5-mm water-stable aggregates, with a significant redistribution of aggregates into micro-aggregates. Long-term winter planted green manure obviously improved C/N ratio and macro-aggregate-associated C and N. The highest contribution to soil fertility was from macro-aggregates of 2-5 mm in most cases.

Contents of soil organic carbon and nitrogen in water-stable aggregates in abandoned agricultural lands in an arid ecosystem of Northwest China

Soil organic matter content in water-stable aggregates（WSA） in the arid ecosystems（abandoned agricultural lands especially） of China is poorly understood. In this study, we examined the WSA sizes and stability, and soil organic carbon（OC） and nitrogen（N） contents in agricultural lands with abandonment ages of 0, 3, 12, 20, 30 and 40 years, respectively, in the Minqin Oasis of Northwest China. The total soil OC and N contents at depths of 0–20, 20–40 and 40–60 cm in abandoned agricultural lands were compared to those in cultivated land（the control）. Agricultural land abandonment significantly（P0.25 mm） as the age of agricultural land abandonment increased. The effect of abandonment ages of agricultural lands on MWD was determined by the changes of OC and N accumulation in WSA sizes ＆gt;2 mm. The total OC and N contents presented a stratification phenomenon across soil depths in this arid ecosystem. That is, both of them decreased significantly at depths of 0–20 and 40–60 cm while increased at the depth of 20–40 cm. The WSA sizes ＆lt;0.053 mm had the highest soil OC and N contents（accounting for 51.41%–55.59% and 42.61%–48.94% of their total, respectively）. Soil OC and N contents in microaggregates（sizes 0.053–0.25 mm） were the dominant factors that influenced the variations of total OC and N contents in abandoned agricultural lands. The results of this study suggested that agricultural land abandonment may result in the recovery of WSA stability and the shifting of soil organic matter from the silt＋clay（＆lt;0.053 mm） and microaggregate fractions to the macroaggregate fractions. However, agricultural land abandonment did not increase total soil OC and N contents in the short-term.

Application o f 210 Pb, Carbon and Nitrogen Isotope in Tracing of Changes and Burial Effect of Endogenous and Terrigenous Organic Matter in the Bering Sea over the Past 100 Years

By using the multi-tube sediment samples collected from NB01 station in the Bering Sea during the period of the fourth Chinese Arctic research expedition in 2010,as well as the dating technique of radioisotope ^(210)Pb in sedimentary strata,based on the exponential decay trend of ^(210)Pb exwith the column sample depth,relative stable modern sedimentary environment in the investigation area was reflected. The results show that the deposition rate of modern marine sediment S was 0. 27 cm/a,and correlation coefficient R was 0. 96( n = 17),while time span was 107 a(1903-2010). Meanwhile,carbon,nitrogen and isotopes were used to trace the sources of materials in the Bering Sea. It is found that the content of organic carbon and nitrogen in the column samples from NB01 station in the Bering Sea was 1. 18%-1. 80% and 0. 16%-0. 28% respectively,and C/N ratio ranged from 5. 13 to 8. 31. Total organic carbon( TOC) and total organic nitrogen( TON) were preserved well in sedimentary strata and had good consistency,showing that sources of organic matter were consistent. Moreover,their changes positively correlated with^(210)Pb. Organic carbon isotope( δ13 C) ranged from-22. 45‰ to-21. 82‰,and its changing trend was similar to that of C/N ratio; organic nitrogen isotope(δ15 N) varied from 7. 53‰ to 8. 54‰,indicating that organic matter was mainly from remaining marine organisms after being decomposed,into which a certain quantity of terrigenous materials were mixed. In the 100 years,the overall trend of terrigenous materials became increasingly obvious,showing that the input and burial of organic carbon from seas and land in the Bering Sea were changing. According to the burial rate of surface sediment and content of organic carbon,the apparent burial flux of sedimentary organic carbon in the column sample from NB01 station in the Bering Sea was estimated,about 1 450 mmol C/( m^2·a). It is suggested that the high burial flux of sedimentary organic carbon in the regions was mainly related to the high primary productivity of the water body,the high output efficiency of organic carbon in the photic zone,favorable preservation and metabolic mechanism of organic matter,and high deposition rate.

Effect of dietary stable isotopic ratios of carbon and nitrogen on the extent of their incorporation into tissues of rats

This study was conducted to investigate the effect of different dietary ratios of 13C to 12C or 15 N to 14N on their relative incorporation into tissues. Eighty male rats were used in two 21-day feeding trials in which they were fed diets with either high δ13C levels （δ13C=-13.89%o and δ15N =2.37%o in experiment 1 and δ13C=-19.34%o and δ15N = 4.73%o in experiment 2） or low 613C levels （δ13C =-17.90%o and δ15N = 3.08%o in experiment 1 and δ13C =-21.76%o and δ15N = 0.53‰ in experiment 2）, meanwhile, the dietary δ15N levels were designed to two ranks Blood, liver, adipose and muscle tissues were collected on day 0, 3, 7, 14, and 21 for determination of 13C, 12C, 15N and 14 N isotopes. Rat growth rate, antioxidant capacity and metabolic parameters were also assessed. The results indicate that adipose tissue tend to deplete 13C before the stable isotopic ratios achieved final equilibrium. Therefore, feeds with different isotopic signatures had different incorporation rates into tissues. Low dietary 13C levels decreased tissue δ13C values whereas high dietary 13C levels did not alter tissue δ13C values during the 21-d experiment. Blood δ15N values were a reliable parameter in assessing the relative contribution of dietary nitrogen to tissues. This study revealed a relationship between dietary tissues. However, more studies are needed to illustrate the sotopic signatures and their incorporation rates into rat mechanism through which dietary isotopic ratios nfiuence the extent of isotopic incorporation into the tissues

Analysis of carbon and oxygen stable isotopes in carbonate rocks by the laser micro-sampling technique

The analysis of stable isotopes of carbon and oxygen in different carbonate rocks by the phosphoric acid method is not easier than that by the laser sampling method developed in recent years, which optically focuses laser beams with sufficient energy on a micro area of a thin section in a vacuum sample box via microscope. CO 2 produced by heating decomposition of carbonate was purified by the vacuum system, and the stable isotopic values of carbon and oxygen were calculated and analyzed on a mass spectrometer. This paper adopted the laser micro-sampling technique to analyze the stable isotopes of carbon and oxygen in dolomite, carbonate cement, stromatolite and different forms of dawsonite (donbassite). Results indicated that the laser micro-sampling method is effective in analyzing carbonate composition and could be a convincing proof for justification on carbonate composition analysis.

Assimilation of carbonate country rock by the parent magma of the Panzhihua Fe-Ti-V deposit(SW China):Evidence from stable isotopes

The Panzhihua intrusion in southwest China is part of the Emeishan Large Igneous Province and host of a large Fe-Ti-V ore deposit.During emplacement of the main intrusion,multiple generations of mafic dykes invaded carbonate wall rocks,producing a large contact aureole.We measured the oxygen-isotope composition of the intrusions,their constituent minerals,and samples of the country rock.Magnetite and plagioclase from Panzhihua intrusion haveδ18O values that are consistent with magmatic equilibrium, and formed from magmas withδ18O values that were 1-2‰higher than expected in a mantle-derived magma.The unmetamorphosed country rock has highδ18O values,ranging from 13.2‰（sandstone） to 24.6-28.6‰（dolomite）.The skarns and marbles from the aureole have lowerδ18O andδ13C values than their protolith suggesting interaction with fluids that were in exchange equilibrium with the adjacent mafic magmas and especially the numerous mafic dykes that intruded the aureole.This would explain the alteration ofδ18O of the dykes which have significantly higher values than expected for a mantle-derived magma.Depending on the exactδ18O values assumed for the magma and contaminant, the amount of assimilation required to produce the elevatedδ18O value of the Panzhihua intrusion was between 8 and 13.7 wt.%,assuming simple mixing.The exact mechanism of contamination is unclear but may involve a combination of assimilation of bulk country rock,mixing with a melt of the country rock and exchange with CO2-rich fluid derived from decarbonation of the marls and dolomites.These mechanisms,particularly the latter,were probably involved in the formation of the Fe-Ti-V ores.

Spatial distributions of organic carbon and nitrogen and their isotopic compositions in sediments of the Changjiang Estuary and its adjacent sea area

The spatial distribution patterns of total organic carbon and total nitrogen show significant correlations with currents of the East China Sea Shelf. Corresponding to distributions of these currents, the study area could be divided into four different parts. Total organic carbon, total nitrogen, and organic carbon and nitrogen stable isotopes in sediments show linear correlations with mean grain size, respectively, thus ＂grain size effect＂ is an important factor that influences their distributions. C/N ratios can reflect source information of organic matter to a certain degree. In contrast, nitrogen stable isotope shows different spatial distribution patterns with C/N and organic carbon stable isotope, according to their relationships and regional distributions. The highest contribution （up to 50%） of terrestrial organic carbon appears near the Changjiang Estuary with isolines projecting towards northeast, indicating the influence of the Changjiang dilution water. Terrestrial particulate organic matter suffers from effects of diagenesis, benthos and incessant inputting of dead organic matter of plankton, after depositing in seabed. Therefore, the contribution of terrestrial organic carbon to particulate organic matter is obviously greater than that to organic matter in sediments in the same place.

Applications of Light Stable Isotopes(C, O, H) in the Study of Sandstone Diagenesis: A Review

This article reviews the applications of light stable isotope, including carbon, oxygen and hydrogen, in thestudies on origin and formation temperature of authigenic carbonate, quartz and clay minerals. Theoretical knowledge andanalytical methods for major light stable isotopes are introduced in detail. Negative and positive δ^(13)C values indicatesignificant differences on the origin of carbonate cements. The δ^(18)O value is an effective palaeotemperature scale forauthigenic minerals formation. Various fractionation equations between δ^(18)O and temperature are proposed for carbonatecements, quartz cements and clay minerals, whose merit and demerit, applicable conditions are clarified clearly. Clumpedisotope analysis can reconstruct the temperature of carbonate precipitation with no requirement on the δ^(18)O of initial waters,which makes temperature calculation of carbonate cements formation more convenient and accurate. Hydrogen and oxygenisotopes mainly reflect the origin of diagenetic fluid for clay mineral formation, providing reliable evidence for diageneticenvironment analysis. This work aims at helping researchers for better understanding the applications of light stable isotopein sandstone diagenesis.