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金葡菌L型感染大鼠后肿瘤内癌基因、抑癌基因表达的研究 被引量:3
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作者 夏佩莹 刘勇 +1 位作者 汪万英 姚敏 《中国人兽共患病杂志》 CSCD 北大核心 2003年第2期63-66,共4页
目的 探讨细菌L型感染后实验动物肿瘤细胞中癌基因、抑癌基因的表达。方法 用金葡菌及其L型以静脉、阴道方式感染Wistar大鼠 ,观察大鼠自发性肿瘤发生情况 ;并用金葡菌L型、癌基因及细胞凋亡抑制基因DNA及特异性抗体对异常增生组织及... 目的 探讨细菌L型感染后实验动物肿瘤细胞中癌基因、抑癌基因的表达。方法 用金葡菌及其L型以静脉、阴道方式感染Wistar大鼠 ,观察大鼠自发性肿瘤发生情况 ;并用金葡菌L型、癌基因及细胞凋亡抑制基因DNA及特异性抗体对异常增生组织及肿瘤细胞作核酸原位杂交和免疫组化。结果 细菌L型感染组大鼠出现绒癌 3例、子宫平滑肌肉瘤 2例、各感染组中共发现雌鼠胎盘滋养层细胞异常增生 9例 ,肿瘤细胞内有金葡菌DNA及癌基因表达。结论 细菌L型感染与实验动物肿瘤细胞中癌基因的表达可能有一定关系。 展开更多
关键词 金葡菌L型感染 大鼠 肿瘤 癌基因 抑癌基因 表达
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Microbiological assessment of street foods at the point of sale in Maputo(Mozambique) 被引量:1
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作者 Acacio Salamandaneu Ana Carla Silva +1 位作者 Luisa Brito Manuel Malfeito-Ferreira 《Food Quality and Safety》 SCIE CSCD 2021年第1期22-30,共9页
Objectives:The aim of this study was to assess the microbiological quality and safety of street food sold in the main streets and informal markets of Maputo,the capital of Mozambique.Materials and Methods:From 83 diff... Objectives:The aim of this study was to assess the microbiological quality and safety of street food sold in the main streets and informal markets of Maputo,the capital of Mozambique.Materials and Methods:From 83 different vendors selling different types of foods,83 samples of ready-to-eat(RTE)street food were analyzed.Mesophiles,Escherichia coli and total coliforms were used as quality and hygiene indicators.Listeria monocytogenes(L.monocytogenes)Salmonella and coagulase-positive staphylococc were used as food safety indicators.Results:High proportions of unsatisfactory food samples were found in both traditional hot(76.7%)and cold(75%)foods.L.monocytogenes and Salmonella were tested negative in this survey.However,when coagulase-positive staphylococci was used as a food safety indicator,approximately 25%(23/83)of the food samples analyzed were classified as unsatisfactory/potentially hazardous.Conclusions:These results,showing that street food sold in Maputo clearly requires adequate sanitary conditions for its preparation and sale,contribute to the development of good manufacturing practices(GMP)for street food in Maputo,Mozambique.This is the first report on the microbiological quality and safety of street food in Mozambique. 展开更多
关键词 ready-to-eat street food Escherichia coli coagulase-positive staphylococcr good manufacturing practices good hygiene practices
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