Effects of Curing Techniques on Starch Contents of Leaves in Different Tobacco Parts with Varying Maturity Degrees

[Objective] The aim was to explore effects of different curing techniques on starch contents of tobaccos from different growth parts and with varying maturity degrees in order to reduce starch content of Yunnan tobacco. [Method] Compar- isons were conducted on tobaccos from upper, middle and down parts （immature, premature, mature and excessive mature） by low temperature and low humidity cur- ing technique, moderate temperature and moderate humidity curing technique, mod- erate temperature and high humidity curing technique to measure starch contents before and after curing. [Result] The results showed starch content of tobacco was increasing upon growth part. Low temperature and low humidity curing technique has poor effects on reduction of starch content; moderate temperature and moderate humidity curing technique improves starch content; moderate temperature and high humidity curing technique dramatically reduces starch content of tobaccos. [Conclu- sion] With consideration of tobacco appearance, inner quality and characters, moder- ate temperature and moderate humidity curing technique is recommended for tobac- co production.

Identification of QTLs for Starch Content in Sweetpotato(Ipomoea batatas(L.) Lam.)

Sweetpotato （Ipomoea batatas （L.） Lam.） breeding is challenging due to its genetic complexity. In the present study, interval mapping （IM） and multiple quantitative trait locus （QTL） model （MQM） analysis were used to identify QTLs for starch content with a mapping population consisting of 202 F1 individuals of a cross between Xushu 18, a cultivar susceptible to stem nematodes, with high yield and moderate starch, and Xu 781, which is resistant to stem nematodes, has low yield and high starch content. Six QTLs for starch content were mapped on six linkage groups of the Xu 781 map, explaining 9.1-38.8% of the variation. Especially, one of them, DMFN 4, accounted for 38.8% of starch content variation, which is the QTL that explains the highest phenotypic variation detected to date in sweetpotato. All of the six QTLs had a positive effect on the variation of the starch content, which indicated the inheritance derived from the parent Xu 781. Two QTLs for starch content were detected on two linkage groups of the Xushu 18 map, explaining 14.3 and 16.1% of the variation, respectively. They had a negative effect on the variation, indicating the inheritance derived from Xu 781. Seven of eight QTLs were co-localized with a single marker. This is the first report on the development of QTLs co-localized with a single marker in sweetpotato. These QTLs and their co-localized markers may be used in marker-assisted breeding for the starch content of sweetpotato.

Molecular Markers Associated with Starch Content and Implications for Sugarcane Introgression Breeding Using Saccharum spontaneum

Introgression with Saceharum spontaneum intended to broaden the genetic base of sugarcane resulted in an increase in desirable genes for broader adapt ability as well as undesirable genes for high starch content. Markers could provide a quick and efficient method of screening parental genotypes for low starch. The objectives of this study were to identify potential markers associated with starch in S. spontaneum population and evaluate their potential for screening for starch content. Data for starch content and （simple sequence repeats） SSR markers were collected from 51 S. spontaneum clones grown in replicated pots. The mixed procedure of statistical analysis system （SAS） was used to determine markers significantly associated with starch. Thirty-nine out of 357 polymorphic markers were significantly （P 〈 0.05） associated with starch content. Eighteen were positively associated and 21 were negatively associated. The presence of a positive marker produced 39% more starch than absence while the absence of a negative marker produced 57% more starch than presence. Selecting parents using negative markers may be more efficient than selecting using positive markers.

Genome-wide association study for starch content and constitution in sorghum(Sorghum bicolor(L.) Moench)

Starch is the most important component in endosperm of sorghum grain.Usually,two types of starch are present:amylose(AM)and amylopectin(AP).The levels of AM and AP contents play a significant role in the appearance,structure,and quality of sorghum grains and in marketing applications.In the present study,a panel of 634 sorghum(Sorghum bicolor(L.)Moench)accessions were evaluated for starch,AM,and AP contents of grain,which included a mini core collection of 242 accessions from the International Crops Research Institute for the Semi-Arid Tropics(ICRISAT)in India,and 252 landraces and 140 cultivars from China.The average starch content was 67.64%and the average AM and AP contents were 20.19 and 79.81%,respectively.We developed a total of 260000 high-confidence single nucleotide polymorphism(SNP)markers in the panel of 634 accessions of S.bicolor using specific locus amplified fragment sequencing(SLAF-seq).We performed genome-wide association studies(GWAS)of starch,AM,and AM/AP of grain and SNP markers based on a mixed linear model(MLM).In total,70 significant association signals were detected for starch,AM,and AM/AP ratio of grain with P<4.452×10^-7,of which 10 SNPs were identified with significant starch,51 SNPs were associated with AM,and nine SNPs were associated with the AM/AP ratio.The Gene Ontology(GO)analysis identified 12 candidate genes at five QTLs associated with starch metabolism within the 200-kb intervals,located on chromosomes 1,5,6,and 9.Of these genes,Sobic.006G036500.1 encodes peptidyl-prolyl cis-trans-isomerase CYP38 responsible for hexose monophosphate shunt(HMS)and Sobic.009G071800 encodes 6-phospho-fructokinase(PFK),which is involved in the embden-meyerhof pathway(EMP).Kompetitive allele specific PCR(KASP)markers were developed to validate the GWAS results.The C allele is correlated with a high starch content,while the T allele is linked with a low level of starch content,and provides reliable haplotypes for MAS in sorghum quality improvement.

Mapping QTL underlying tuber starch content and plant maturity in tetraploid potato

Tuber starch content and plant maturity are two important agronomic traits of potato. To investigate the complex genetic basis of these traits in the cultivated potato, as well as the relationship between them, we developed a linkage map in a tetraploid population of 192 clones derived from the cross Longshu 8 × Zaodabai and mapped quantitative trait loci(QTL) for tuber starch content and plant maturity using data collected in three diverse environments over two years. We detected eleven QTL for tuber starch content distributed on seven chromosomes, of which four, on chromosomes I, II, and VIII, were expressed in at least three environments. For plant maturity, we identified six QTL on chromosomes II, IV,V, VII, and XI, one of which, on chromosome V, showed LOD peaks ranging from 45.2 to 62.5 cM and explained 21.6%–26.6% of phenotypic variation was expressed in five of the six environments. Because the reproducible QTL for plant maturity and tuber starch content mapped to different chromosomes and neither overlapping QTL, nor any genetic interaction between QTL were detected, we infer that tuber starch content and plant maturity are controlled by independent genetic loci. This inference supports the prospect of breeding potato for both early maturity and high starch content.

Effects of Bacterial Manure from Cassava Alcohol Fermentation Mash on Yield and Starch Content of Cassava

In order to provide a scientific basis for the utilization of cassava alcohol fermentation mash and the high-yield and high-efficiency cultivation of cassava,compound microbial inoculum was inoculated into the cassava alcohol fermentation mash to prepare bacterial manure,which was then applied to two cassava varieties,Nanzhi 199 and SC 205 to explore the optimum application amount,application method and application concentration. The results showed that when the inoculation amount of the compound microbial inoculum was 2‰ of the mass of the mash,the requirements of industrial production of liquid bacterial manure and agricultural application can be met,saving costs. After the emergence of cassava seedlings,if they were drenched with the bacterial manure of cassava alcohol fermentation mash through roots once according to the amount of 67. 5 t/ha and irrigated with the bacterial manure once every 15 d under drought conditions,both the yield and quality of cassava can be improved significantly. The yield and starch content of Nanzhi 199 reached 26 196. 23 kg/ha and 30. 32%,36. 57% and4. 75 percentage points higher than those of CK. The yield and starch content of SC 205 reached 41 126. 06 kg/ha and 23. 74%,99. 25% and3. 19 percentage points higher than those of CK. Under the application modes of foliar spray,root irrigation and foliar spray + root irrigation,the fertilization effect was more obvious. When undiluted bacterial manure of cassava alcohol fermentation mash was applied according to the amount of 52. 5 t/( ha·time),the fertilization effect of the application mode of foliar spray + root irrigation was the best. The bacterial manure from cassava alcohol fermentation mash can be directly applied without dilution,and the fertilizer efficiency will be reduced significantly after dilution.

A plastidic ATP/ADP transporter gene, IbAATP, increases starch and amylose contents and alters starch structure in transgenic sweetpotato

A plastidic adenosine triphosphate（ATP）/adenosine diphosphate（ADP） transporter（AATP） is responsible for importing ATP from the cytosol into plastids. In dicotyledonous plants, increasing ATP supply is a potential way to facilitate anabolic synthesis in heterotrophic plastids. In this study, a gene encoding the AATP protein, named Ib AATP, was isolated from sweetpotato（Ipomoea batatas（L.） Lam.）. Transcripts of Ib AATP were predominantly detected in the storage roots and leaves and were induced by exogenous sucrose and subjected to circadian rhythm. Transient expression of Ib AATP in tobacco and onion epidermal cells revealed the plastidic localization of Ib AATP. The overexpression of Ib AATP in sweetpotato significantly increased the starch and amylose contents and led to enlarged starch granules. The IbA ATP-overexpressing plants showed altered fine structure of amylopectin, which contained an increased proportion of chains with a degree of polymerization（DP） of 10–23 and a reduced number of chains with a DP of 5–9 and 24–40. In addition, starch from the transgenic plants exhibited different pasting properties. The transcript levels of starch biosynthetic genes, including Ib AGP, Ib GBSSI, Ib SSIIV, and Ib SBE, were differentially regulated in the transgenic plants. These results revealed the explicit role of Ib AATP in the starch biosynthesis of sweetpotato and indicated that this gene has the potential to be used to improve starch content and quality in sweetpotato and other plants.

Effects of RNAi Silencing of SSIII Gene on Phosphorus Content and Characteristics of Starch in Potato Tubers

The sense and antisense fragments of the soluble starch synthase （SSII1） gene and the intron fragment of somatic embryogenesis receptor-like kinase （SERK1） gene were cloned from potato using PCR techniques. The RNAi plant expression vectors pBI-SSIII-RNAi and pBIC-SSIII-RNAi were constructed which containing fusion fragment of ＂sense fragment-intron-antisense fragment＂ driven by the constitutive expression promoter CaMV 35S and the tuber-specific expression promoter CIPP, respectively. The putative transgenic plants of potato cultivars Kexin-1 and Kexin-4 were obtained using Agrobacterium-mediated transformation method. PCR assay showed that the interference fragment of SSlll gene was integrated into potato genome. The RT-PCR analysis showed that the expression of SSlll gene was repressed apparently on the transcription level. Starch granules of the transgenic potato plants were different in morphology and became cracked in starch granule centre compared with the non-transgenic control plants. The amylose content of starch was increased by 2.68-29.05%, amylopectin to amylose ratio of starch had declined significantly, and the phosphorus content of the starch of the transgenic plants was reduced 9.94-58.36% compared with control plants. The results could provide certain foundation for improvement of potato starch quality.

Effect of Different Moisture Contents on Crystallized State of Microwave Modified Tapioca Starch

Effect of microwave modification on tapioca starch at different moisture contents was studied with polarized light microscope. It was observed that loss of birefringence was initiated at hilum of granule. The polarization cross of most granules had become somewhat unclear, and reduction in clarity of the polarization cross was proportioned with the moisture contents of native starch as modification by microwave. When the moisture contents reached to 35.0%, the polarization cross of most granules was lost, and the critical point of native starch was 30% in microwave modification.

96份谷子种质资源淀粉多样性及优异资源鉴选利用

采用双波长微量法对国内有代表性的96份谷子种质资源的直链淀粉和支链淀粉含量进行了测定,分析其淀粉多样性与直/支比变异类型。结果表明,各品种直链淀粉含量介于0~24.91%,平均值为15.66%,支链淀粉含量介于4.77%~34.26%,平均值为14.67%;44份育成品种和52份地方品种直链淀粉含量变化幅度分别为8.66%~21.81%和0~24.91%;96份谷子的直/支比变化范围为0~3.60,平均为1.18,其中直/支比<0.05的地方品种仅2个,而直/支比>0.33的品种93个,占总数的96.87%。直链淀粉含量小于3.5%的糯性品种3个,其中完全糯性品种2个;高直链淀粉品种4个,直链淀粉含量介于12.0%~18.0%的种质有60份,占总数的62.50%。筛选出适合加工糍粑、汤圆类糯质食品的地方品种3个,为红毛谷、三爪谷和酒谷;可用于加工米粉等高膳食纤维的地方品种4个,分别为金皇谷、金棒子、茄谷和山西白米。96份谷子种质资源中地方品种直链淀粉变异类型较为丰富,育成品种直链淀粉遗传基础相对单一。

不同热处理条件下橡胶木淀粉含量与抗蠹虫性能关系研究

为克服橡胶木易发生蠹虫危害的缺陷,采用不同热处理温度(90、120、150℃和180℃)和不同热处理时间(2、4 h和6 h)对橡胶木进行热处理,利用分光光度计考察不同温度和时间条件下热处理橡胶木中的淀粉含量变化,并分析蠹虫对橡胶木的蛀蚀程度与淀粉含量之间的关系。结果表明:热处理温度和处理时间对橡胶木中的淀粉含量有显著影响,且随着热处理温度的升高和热处理时间的延长,淀粉含量逐渐下降,从12.79%降至1.25%。在橡胶木抗蠹虫试验中,热改性处理后橡胶木的抗蠹虫效果均优于对照材,且橡胶木淀粉含量越低,其抗蠹虫效果越好。在实际生产过程中,在120℃下处理6 h为生产热处理橡胶木的优化条件,既能刚好维持橡胶木抗蠹虫的最低要求,也能降低热处理过程中木材强度的损失。

紫外辐照及甲基磺酸乙酯诱变对马铃薯早熟品种淀粉含量和产量性状的影响

针对黑龙江省现有马铃薯早熟品种淀粉含量较低的问题,通过紫外辐照和甲基磺酸乙酯浸泡2种方式,对东农303和中龙薯1号的组培苗进行处理,经过3 a的种植、调查和数据采集,确定诱变方式对不同品种马铃薯变异程度和诱变方向的影响,以改良获得早熟且高淀粉含量的马铃薯品种。结果表明,诱变后,整体变化表现为无变化>抑制>促进,其中东农303更易发生变异,紫外辐照的诱变效果更佳。在组培苗阶段,多数处理显示为抑制作用;在原原种阶段,紫外辐照有利于东农303产量的提升和中龙薯1号淀粉含量的增加;在原种阶段,甲基磺酸乙酯浸泡对东农303的商品性有提升作用,而2种诱变方式对中龙薯1号的产量和结薯数量均有明显抑制作用,紫外辐照的影响更为显著。在紫外辐照处理中,2个品种均发现了1个淀粉含量明显提升的单株,2个单株的发现将有利于黑龙江省不同熟期品种的搭配种植,减少完全依赖晚熟品种所带来的种植风险,并可有效提升产业效益。

Natural variation of an autophagy-family gene among rice subspecies affects grain size and weight

Elucidating the genetic basis of natural variation in grain size and weight among rice varieties can help breeders develop high-yielding varieties.We identified a novel gene,GW3a(Grain Weight 3a)(LOC_Os03g27350),that affects rice grain size and weight.gw3a mutants showed higher total starch content and dry matter accumulation than the wild type(WT),Nipponbare,suggesting that GW3a negatively regulates grain size and weight.Moreover,our study found that GW3a interacted with OsATG8 by cleaving it,suggesting that GW3a may be involved in the assembly of autophagosomes and starch degradation in plants.The haplotype analysis of GW3a showed functional differences between indica and japonica rice.Taken together,we conclude that GW3a is expressed in the autophagosome pathway regulating starch metabolism in rice,affecting yield-related traits,such as grain size,grain weight and thousand grain weight(TGW).Our findings also shed new light on autophagy-mediated yield trait regulation,proposing a possible strategy for the genetic improvement of high-yield germplasm in rice.

9个不同使用类型木薯品系在合浦县的适应性研究

为筛选适宜在合浦县推广的木薯品系,本研究以主栽品种‘南植199’为对照,对9个木薯新品系的生物学特性进行了观察,并测定其农艺性状和经济性状,同时进行相关性分析。结果表明,9个品系分枝角度小、块根长度适中和主茎高度中等以上;‘RC4’的块根产量显著高于对照,其余8个品系与对照差异不显著;‘RC4’等8个品系的淀粉产量高于对照;9个品系的淀粉含量高于对照,其中‘RC3’等5个品系的淀粉含量显著或极显著高于对照;9个木薯品系的直链淀粉含量显著或极显著低于对照‘;RC6’等6个品系的氢氰酸含量低于50 mg/kg。相关性分析结果表明:块根产量与生长中期株高、成熟期株高、块根直径呈极显著正相关,与茎粗呈显著正相关;淀粉含量与生长中期株高、成熟期株高、茎粗呈显著正相关;淀粉产量与生长中期株高、成熟期株高、茎粗、块根直径、块根产量呈极显著正相关,与淀粉含量呈显著正相关。综合来看,这9个木薯新品系的综合表现良好,适合在合浦县进行推广。其中‘,RC3’、‘RC4’和‘RC7’可作为加工型品系;‘RC5’和‘RC10’可作为食用品系;‘RC2’、‘RC6’、‘RC11’和‘RC12’可作为鲜食加工兼用品系;‘RC2’和‘RC5’作为食用品系具有很好的推广前景。

不同温度对羊肚菌淀粉代谢的调控研究

研究了不同温度对淀粉培养基中羊肚菌菌丝生长、淀粉酶活[腺苷二磷酸葡萄糖焦磷酸化酶(AGPase)、α-淀粉酶、β-淀粉酶、淀粉分支酶(DBE)、淀粉脱分支酶(SBE)]、淀粉含量(直链淀粉、支链淀粉)和淀粉相关酶基因表达的影响。结果表明:在10—25℃温度条件下,随着温度的增加,淀粉培养基上菌丝生长速度加快,菌丝浓密度和菌丝尖端分叉增加;AGPase、DBE、α-淀粉酶、β-淀粉酶在20℃下活性最高,SBE则在10℃下活性最高。直链淀粉含量和支链淀粉剩余含量在10℃下最高。相关性分析结果显示,温度与α-淀粉酶活性和SBE活性相关性不显著,与β-淀粉酶活性、AGPase活性、DBE活性呈显著负相关,与直链淀粉含量和支链淀粉含量呈极显著负相关。综上,在15—20℃温度条件下,羊肚菌对培养基中的淀粉代谢能力最强,10℃低温或者25℃高温都不利于羊肚菌对培养基质中淀粉的利用。研究可为羊肚菌的菌种制作和人工种植提供理论支撑。

甘薯抗性淀粉研究进展

甘薯是重要的淀粉作物,其块根中抗性淀粉(RS)含量较高,具备良好的产品开发潜力。要进一步促进甘薯RS的应用和推广,不仅需要筛选或培育出RS含量更高的甘薯品种,还需要加大开发甘薯深加工淀粉产品。除调控淀粉合成相关基因的表达外,生产实际中还可通过调整栽培措施等方式提高甘薯RS的含量。通过化学修饰法、物理法和酶解法等方法可制备甘薯RS产品,也能提高产品中RS的含量。本文系统综述了近年来甘薯淀粉的保健效果、RS含量的测定方法、影响甘薯淀粉中RS含量的因素、RS的制备方法及其特性等方面的进展,为未来甘薯中RS的制备、产量提高、产品开发和推广提供参考。

优质蛋白玉米‘荃玉9号’主要营养品质性状的QTL定位

油分、蛋白质和淀粉是玉米籽粒的主要营养成分,同时也是玉米重要的品质性状。为探索更多玉米品质相关的QTL,本研究以优质蛋白玉米‘荃玉9号’构建的F2:3群体为材料,通过方差分析和QTL定位方法,对玉米籽粒品质性状(包括油分、蛋白质和淀粉含量)进行了遗传分析。在新都和简阳2地共检测到24个品质性状QTL,其中仅与油分含量、蛋白质含量和淀粉含量相关的QTL分别为9、9和2个,与多个品质性状相关的QTL为4个,QTL在10条染色体上均有分布,单个QTL可解释的表型贡献率为1.92%~34.43%。染色体上Bin7.01(6399330~8305989)的QTL在2个环境同时被检测到,可以解释12.68%~18.13%的表型变异且有加性效应,是控制玉米油分含量的主效QTL。本研究为玉米品质改良的分子辅助育种工作提供了基础数据资料,为后续的育种实践提供了有力支撑。

中高温大曲发酵中小麦籽粒营养组分的动态变化分析

该研究利用离子色谱和高效液相色谱等方法,研究了中高温大曲不同发酵时期小麦营养成分的变化及其与微生物群落演替间关系。结果表明,曲皮和曲心分别消耗了直链淀粉含量的39.08%和49.12%,支链淀粉含量的84.43%和81.35%,A型淀粉粒的6.41%和26.92%,B型淀粉粒的66.67%和83.33%。麦谷蛋白含量从8 mg/g降为4.73 mg/g(曲皮)和3.53 mg/g(曲心)。黄酮含量从0.08 mg/g增加到0.12 mg/g(曲皮)和0.29 mg/g(曲心)。纤维素含量总体呈下降趋势,但曲心后期有平缓上升;阿拉伯糖和蔗糖含量增加,葡萄糖含量降低。曲心游离氨基酸含量增加幅度高于曲皮,其中谷氨酸含量最高。冗余分析表明18个品质指标与9个细菌属和11个真菌属的相对丰度显著相关(P<0.05),其中直链淀粉含量与叶杆菌属(Phyllobacterium),葡萄糖、果糖、支链淀粉含量与醋酸单胞菌属(Acetobacter)、双杆菌属(Undibacterium)、乳杆菌属(Lactobacillus)均显著正相关。因此,具有高支链淀粉和高葡萄糖含量的小麦品种可能更适用于制曲。该研究结果为探究大曲质量控制、小麦品质与大曲品质间关系,以及选育专用型原料(小麦)品种提供参考。

Effects of Weak Light on Starch Accumulation and Starch Synthesis Enzyme Activities in Rice at the Grain Filling Stage

Dynamic changes of starch, amylose, sucrose contents and the activities of starch synthesis enzymes under shading treatments alter flowering were studied using two rice varieties IR72 （indica） and Nipponbare （japonica） as materials. Under shading treatments, the starch, amylose and sucrose contents decreased, while ADP-glucose pyrophosphorylase （ADPGPPase） activity only changed a little, soluble starch synthase activity and granule bound starch synthase activity decreased, soluble starch branching enzyme （SSBE, Q-enzyme） activity and granule bound starch branching enzyme （GBSBE, Q-enzyme） activity increased, and starch debranching enzyme （DBE, R-enzyme） activity varied with varieties. Correlation analyses showed that the changes of starch content were positively and significantly correlated with the changes of sucrose content in the weak light. Both ADPGPPase activity and SSBE activity were positively and significantly correlated with starch accumulation rate. It was implied that the decline of starch synthase activities was related to the decrease of starch content and the increase of the activity of starch branching enzyme played an important role in the decrease of the ratio of amylose to the total starch under the weak light.

不同微生物菌剂浸种对马铃薯生长发育及薯田土壤养分的影响

【目的】探究不同微生物菌剂浸种对马铃薯生长发育及薯田土壤养分的影响,为内蒙古自治区马铃薯绿色、可持续种植提供参考。【方法】以马铃薯品种V7为试验材料,分别采用蒸馏水(CK)、Bacillus sp.188菌剂(T1)、Pseudomonas sp.BP16菌剂(T2)和Streptomyces rochei D74菌剂(T3)浸种30 min,测定马铃薯种植后不同生长时期产量、块茎淀粉含量、各器官干物质量、单株养分积累量,叶片叶绿素含量、硝酸还原酶活性以及土壤铵态氮、硝态氮、速效磷和速效钾含量。【结果】马铃薯种植后110 d,与CK相比,T2、T3处理马铃薯产量显著提高7.14%、9.16%(P<0.05),块茎淀粉含量显著提高11.84%、16.38%(P<0.05);T3处理马铃薯根、茎、叶、块茎干物质量显著增加26.87%、16.92%、11.77%、13.59%(P<0.05),氮素、磷素、钾素积累量显著提高28.86%、32.78%、30.81%(P<0.05)。种植后70 d,T3处理马铃薯叶片叶绿素含量最高[4.29 mg/(g·FW)],叶片硝酸还原酶活性较CK显著提高15.29%(P<0.05)。马铃薯种植后50 d,T3处理土壤硝态氮含量达到最大值(42.32 mg/kg),速效磷含量较CK显著提高26.17%,(P<0.05),T1、T3处理速效钾含量分别较CK显著提高24.95%、21.65%(P<0.05);种植后70 d,T2处理土壤铵态氮含量较CK显著提高47.03%(P<0.05)。【结论】Streptomyces rochei D74菌剂浸种可提高成熟期马铃薯产量、块茎淀粉含量、各器官干物质量和植株氮素、磷素、钾素积累量以及块茎形成期叶片叶绿素含量,生育前期土壤硝态氮、速效磷和速效钾含量,块茎形成期土壤铵态氮含量,可作为内蒙古自治区马铃薯栽培较适宜施入的微生物菌剂。