The study was conducted to investigate the effect of Rhizobium inoculation and supplementation of phosphorus and potassium on growth and total leaf chlorophyll content to the three released bush bean varieties in nort...The study was conducted to investigate the effect of Rhizobium inoculation and supplementation of phosphorus and potassium on growth and total leaf chlorophyll content to the three released bush bean varieties in northern Tanzania. To achieve this aim, the glasshouse experiment was conducted at Nelson Mandela African Institution of Science and Technology while field experiment were carried out at Tanzania Coffee Research Institute, in Kilimanjaro, Tanzania between April-July 2014. The experiment was laid out in factorial arrangement. Factor I comprised of three bush bean varieties. Factor II involved two inoculation treatments viz 1) inoculation with Rhizobium spp. and 2) without inoculation. Factor III included four fertilizer levels (0 Kg·ha-1 20 Kg K ha-1, 30 Kg P ha-1 and 20 kg·K + 30 Kg P ha-1). Both screen house and field experiments were replicated four times. Plant growth parameters (plant height (cm), number leaves per plant, stem girth (mm)) were measured at 2, 4 and 6 weaks after planting (WAP). The chlorophyll was extracted by using Dimethylsulphoxide (DMSO) and absorbance was determined at 645 and 663nm using UV/Visible spectrophotometer. Results showed that Rhizobium application significantly improved the number of leaves per plant, plant height, pant girth and total leaf chlorophyll content. Furthermore, compared with the zero treatment control, potassium fertilization significantly increased the number of leaves per plant, plant height, pant girth and total leaf chlorophyll content of the three varieties. In general, these parameters were significantly increased with phosphorus supplied at 30 kg/ha. The combination of these supplies at different levels resulted in significant interactions in some parameters and thus indicating need for these inputs in the study area.展开更多
A field and glasshouse experiment was conducted to study the effect of B. japonicum inoculation and phosphorus supplementation on growth and leaf chlorophyll content in soybean. The treatments consisted of B. japonicu...A field and glasshouse experiment was conducted to study the effect of B. japonicum inoculation and phosphorus supplementation on growth and leaf chlorophyll content in soybean. The treatments consisted of B. japonicum inoculation (with & without), phosphorus supplementation at the levels of 0, 20, 40 and 80 kgP·ha-1. Both treatments were replicated four times in a split plot design. The following parameters were measured: plant height (cm), number leaves per plant, number of days to 50% flowering, number of days to 50% pod formation, stem girth and leaf area (LA). Results showed that inoculation with B. japonicum significantly increased plant height, number of leaves, leaf chlorophyll content, stem girth, leaf area (LA) and leaf area index (LAI). However, Phosphorus supplementation had significant effects in some parameters measured. The use of effective strain of B. japonicum and phosphorus supplementation was an efficient way of enhancing the growth of soybean.展开更多
文摘The study was conducted to investigate the effect of Rhizobium inoculation and supplementation of phosphorus and potassium on growth and total leaf chlorophyll content to the three released bush bean varieties in northern Tanzania. To achieve this aim, the glasshouse experiment was conducted at Nelson Mandela African Institution of Science and Technology while field experiment were carried out at Tanzania Coffee Research Institute, in Kilimanjaro, Tanzania between April-July 2014. The experiment was laid out in factorial arrangement. Factor I comprised of three bush bean varieties. Factor II involved two inoculation treatments viz 1) inoculation with Rhizobium spp. and 2) without inoculation. Factor III included four fertilizer levels (0 Kg·ha-1 20 Kg K ha-1, 30 Kg P ha-1 and 20 kg·K + 30 Kg P ha-1). Both screen house and field experiments were replicated four times. Plant growth parameters (plant height (cm), number leaves per plant, stem girth (mm)) were measured at 2, 4 and 6 weaks after planting (WAP). The chlorophyll was extracted by using Dimethylsulphoxide (DMSO) and absorbance was determined at 645 and 663nm using UV/Visible spectrophotometer. Results showed that Rhizobium application significantly improved the number of leaves per plant, plant height, pant girth and total leaf chlorophyll content. Furthermore, compared with the zero treatment control, potassium fertilization significantly increased the number of leaves per plant, plant height, pant girth and total leaf chlorophyll content of the three varieties. In general, these parameters were significantly increased with phosphorus supplied at 30 kg/ha. The combination of these supplies at different levels resulted in significant interactions in some parameters and thus indicating need for these inputs in the study area.
文摘A field and glasshouse experiment was conducted to study the effect of B. japonicum inoculation and phosphorus supplementation on growth and leaf chlorophyll content in soybean. The treatments consisted of B. japonicum inoculation (with & without), phosphorus supplementation at the levels of 0, 20, 40 and 80 kgP·ha-1. Both treatments were replicated four times in a split plot design. The following parameters were measured: plant height (cm), number leaves per plant, number of days to 50% flowering, number of days to 50% pod formation, stem girth and leaf area (LA). Results showed that inoculation with B. japonicum significantly increased plant height, number of leaves, leaf chlorophyll content, stem girth, leaf area (LA) and leaf area index (LAI). However, Phosphorus supplementation had significant effects in some parameters measured. The use of effective strain of B. japonicum and phosphorus supplementation was an efficient way of enhancing the growth of soybean.
