Hydrangea bretschneideri Dipp is a highly popular ornamental plant for garden decoration.Genetic engineering technology has been successfully used in many plant species,but it is limited in Hydrangea.Here we establish...Hydrangea bretschneideri Dipp is a highly popular ornamental plant for garden decoration.Genetic engineering technology has been successfully used in many plant species,but it is limited in Hydrangea.Here we established an efficient regeneration system by using stem segments as explants for the first time.In our study,the plant growth regulators(PGRs)were evaluated at the different regeneration processes,including axillary shoots regeneration and root induction.We found that the optimal concentration for axillary buds’induction was 2.0 mgL^(-1)6-BA and 0.5 mgL^(-1)1 IAA,its highest induction rate was 70%.Moreover,the highest axillary shoots proliferation coefficient was 10.7 on the Murashige and Skoog(MS)medium with 2.0 mgL^(-1)6-benzyladenine(BA),0.2 mgL^(-1)indole-3-butyric acid(IBA),and 1.0 mgL^(-1)gibberellin A3(GA3).The highest frequency of root induction was 80.0±0.06%by culturing the elongated shoots in 1/2 MS medium containing 0.1 mgL^(-1)IBA.In summary,our study will provide an effective technology for large-scale propagation and important pathway for promoting the popularization and application of Hydrangea bretschneideri Dipp.展开更多
Genetic transformation with mature material as the explants could shorten the transgenic period and avoid seed dependence compared with genetic transformation using the epicotyl seedling stem segments as the receptor....Genetic transformation with mature material as the explants could shorten the transgenic period and avoid seed dependence compared with genetic transformation using the epicotyl seedling stem segments as the receptor. Here, we constructed an Agrobacterium tumefaciensmediated transformation for generation of marker-free transgenic plants from navel orange(Citrus sinensis Osbeck) mature stems using a CreloxP recombination system. To efficiently recover the regenerated buds from mature tissues, five recovery methods were compared: in vitro micrografting of 0.1-0.5(1-2 weeks), > 0.5 cm(3-4 weeks) and > 1 cm long lignified bud and in vitro micrografting of explants with a bud and rooting regenerated bud. The data showed that in vitro micrografting of > 1 cm long regenerated bud with expanded leaves after one month of continuous culture for lignification was the optimal solution for plant recovery from mature tissues. Transgenic plants without selectable marker genes were created from navel orange(Citrus sinensis Osbeck) tissue using a transformation vector PLI-35SPR1aCB containing a Cre/loxP system recombination together with genes encoding the selectable marker isopentenyl transferase(IPT) and an anti-bacterial peptide(PR1aCB).Using IPT positive selection, the transformation efficiency determined by PCR was 0.9%, and in total, 20 transgenic plants were obtained.Southern blotting confirmed further their transgenicity. PCR and sequencing analysis demonstrated that both the Cre and IPT genes had been successfully removed from the transgenic plants(deletion efficiency 100%). Over all, using Cre/loxP system recombination together with the IPT positive selection, marker-free transgenic plants can be recovered efficiently from mature tissues of navel orange(Citrus sinensis Osbeck), which provides a potential method for production of transgenic plants from citrus mature tissue.展开更多
为快速准确地提取地面三维激光扫描仪获取林分点云中的单株树木点云,提出一种基于密度的抗噪空间聚类(Density-Based Spatial Clustering of Application with Noise,DBSCAN)的树木分割算法。首先采用高斯滤波对林分点云去噪,在林分点...为快速准确地提取地面三维激光扫描仪获取林分点云中的单株树木点云,提出一种基于密度的抗噪空间聚类(Density-Based Spatial Clustering of Application with Noise,DBSCAN)的树木分割算法。首先采用高斯滤波对林分点云去噪,在林分点云归一化的基础上对林分点云垂直分段,然后采用DBSCAN算法垂直分段聚类,再计算每个垂直分段中每个簇的中心点,根据簇中心点间的距离判定簇间的相邻关系,并由此匹配树干段点云,最后采用RANSAC(Random Sample Consensus)算法对树干段点云拟合直线,并根据点与拟合直线间的距离判定点的归属以实现树木分割。在郁闭度分别为中与高的林分中,所提算法的调和值F范围分别为0.88~0.99与0.72~0.74,基于距离判别的树木分割算法的F范围分别为0.84~0.90与0.73~0.79。所提算法在不同郁闭度的林分点云中均能有效分割单株树木点云,特别是在郁闭度为中的林分中有较好表现,可实现对林分点云的精确树木分割。展开更多
In this study, compatible taper and stem volume equations were developed for Larix kaempferi species of South Korea. The dataset was split into two groups: 80% of the data were used in model fitting and the remaining ...In this study, compatible taper and stem volume equations were developed for Larix kaempferi species of South Korea. The dataset was split into two groups: 80% of the data were used in model fitting and the remaining 20% were used for validation. The compatible MB76 equations were used to predict the diameter outside bark to a specific height, the height to a specific diameter and the stem volume of the species. The result of the stem volume analysis was compared with the existing stem volume model of Larix kaempferi species of South Korea which was developed by the Korea Forest Research Institute and with a simple volume model that was developed with fitting dataset in this study. The compatible model provided accurate prediction of the total stem volume when compared to the existing stem volume model and with a simple volume model. It is concluded that the compatible taper and stem volume equations are more convenient to use and therefore it is recommended to be applied in the Larix kaempferi species of South Korea.展开更多
基金This work is supported by the Grassland Talent Project:The Innovation Team of New Varieties Breeding at the Economic and Ecological Shrub,and the Evaluation of the Economic and Ecological Shrub Resources and New Variety Breeding in Inner Mongolia(No.201702077)。
文摘Hydrangea bretschneideri Dipp is a highly popular ornamental plant for garden decoration.Genetic engineering technology has been successfully used in many plant species,but it is limited in Hydrangea.Here we established an efficient regeneration system by using stem segments as explants for the first time.In our study,the plant growth regulators(PGRs)were evaluated at the different regeneration processes,including axillary shoots regeneration and root induction.We found that the optimal concentration for axillary buds’induction was 2.0 mgL^(-1)6-BA and 0.5 mgL^(-1)1 IAA,its highest induction rate was 70%.Moreover,the highest axillary shoots proliferation coefficient was 10.7 on the Murashige and Skoog(MS)medium with 2.0 mgL^(-1)6-benzyladenine(BA),0.2 mgL^(-1)indole-3-butyric acid(IBA),and 1.0 mgL^(-1)gibberellin A3(GA3).The highest frequency of root induction was 80.0±0.06%by culturing the elongated shoots in 1/2 MS medium containing 0.1 mgL^(-1)IBA.In summary,our study will provide an effective technology for large-scale propagation and important pathway for promoting the popularization and application of Hydrangea bretschneideri Dipp.
基金supported by the Fundamental Research Funds for the Central Universities (Grant No. XDJK 2018B016)the National Natural Sciences Foundation of China (Grant No. 31972393)+1 种基金he earmarked fund for China Agriculture Research System (Grant No. CARS-26)the Natural Science Foundation of Chongqing (Grant No. cstc2020jcyj-msxmX1064)。
文摘Genetic transformation with mature material as the explants could shorten the transgenic period and avoid seed dependence compared with genetic transformation using the epicotyl seedling stem segments as the receptor. Here, we constructed an Agrobacterium tumefaciensmediated transformation for generation of marker-free transgenic plants from navel orange(Citrus sinensis Osbeck) mature stems using a CreloxP recombination system. To efficiently recover the regenerated buds from mature tissues, five recovery methods were compared: in vitro micrografting of 0.1-0.5(1-2 weeks), > 0.5 cm(3-4 weeks) and > 1 cm long lignified bud and in vitro micrografting of explants with a bud and rooting regenerated bud. The data showed that in vitro micrografting of > 1 cm long regenerated bud with expanded leaves after one month of continuous culture for lignification was the optimal solution for plant recovery from mature tissues. Transgenic plants without selectable marker genes were created from navel orange(Citrus sinensis Osbeck) tissue using a transformation vector PLI-35SPR1aCB containing a Cre/loxP system recombination together with genes encoding the selectable marker isopentenyl transferase(IPT) and an anti-bacterial peptide(PR1aCB).Using IPT positive selection, the transformation efficiency determined by PCR was 0.9%, and in total, 20 transgenic plants were obtained.Southern blotting confirmed further their transgenicity. PCR and sequencing analysis demonstrated that both the Cre and IPT genes had been successfully removed from the transgenic plants(deletion efficiency 100%). Over all, using Cre/loxP system recombination together with the IPT positive selection, marker-free transgenic plants can be recovered efficiently from mature tissues of navel orange(Citrus sinensis Osbeck), which provides a potential method for production of transgenic plants from citrus mature tissue.
文摘为快速准确地提取地面三维激光扫描仪获取林分点云中的单株树木点云,提出一种基于密度的抗噪空间聚类(Density-Based Spatial Clustering of Application with Noise,DBSCAN)的树木分割算法。首先采用高斯滤波对林分点云去噪,在林分点云归一化的基础上对林分点云垂直分段,然后采用DBSCAN算法垂直分段聚类,再计算每个垂直分段中每个簇的中心点,根据簇中心点间的距离判定簇间的相邻关系,并由此匹配树干段点云,最后采用RANSAC(Random Sample Consensus)算法对树干段点云拟合直线,并根据点与拟合直线间的距离判定点的归属以实现树木分割。在郁闭度分别为中与高的林分中,所提算法的调和值F范围分别为0.88~0.99与0.72~0.74,基于距离判别的树木分割算法的F范围分别为0.84~0.90与0.73~0.79。所提算法在不同郁闭度的林分点云中均能有效分割单株树木点云,特别是在郁闭度为中的林分中有较好表现,可实现对林分点云的精确树木分割。
基金the Korea Forest Service for funding this research(Project No.S211316L020130)
文摘In this study, compatible taper and stem volume equations were developed for Larix kaempferi species of South Korea. The dataset was split into two groups: 80% of the data were used in model fitting and the remaining 20% were used for validation. The compatible MB76 equations were used to predict the diameter outside bark to a specific height, the height to a specific diameter and the stem volume of the species. The result of the stem volume analysis was compared with the existing stem volume model of Larix kaempferi species of South Korea which was developed by the Korea Forest Research Institute and with a simple volume model that was developed with fitting dataset in this study. The compatible model provided accurate prediction of the total stem volume when compared to the existing stem volume model and with a simple volume model. It is concluded that the compatible taper and stem volume equations are more convenient to use and therefore it is recommended to be applied in the Larix kaempferi species of South Korea.
