The wild Lepista sordida is a kind of precious and rare edible fungus.An excellent strain of it by artificial domestication was obtained,which was high-yield and high in iron content.In this study,high-throughput comp...The wild Lepista sordida is a kind of precious and rare edible fungus.An excellent strain of it by artificial domestication was obtained,which was high-yield and high in iron content.In this study,high-throughput comparative proteomics was used to reveal the regulatory mechanism of its primordium differentiation in the early fruiting body formation.The mycelium before the primordium differentiation mainly expressed high levels of mitochondrial functional proteins and carbon dioxide concentration regulatory proteins.In young mushrooms,the highly expressed proteins were mainly involved in cell component generation,cell proliferation,nitrogen compound metabolism,nucleotide metabolism,glutathione metabolism,and purine metabolism.The differential regulation patterns of pileus and stipe growth to maturity were also revealed.The highly expressed proteins related to transcription,RNA splicing,the production of various organelles,DNA conformational change,nucleosome organization,protein processing,maturation and transport,and cell detoxification regulated the pileus development and maturity.The proteins related to carbohydrate and energy metabolism,large amounts of obsolete cytoplasmic parts,nutrient deprivation,and external stimuli regulated the stipe development and maturity.Multiple CAZymes regulated nutrient absorption,morphogenesis,spore production,stress response,and other life activities at different growth and development stages.展开更多
基金funded by the Shandong Edible Fungus Agricultural Technology System(SDAIT-07-02)the National Natural Science Foundation of China(Grant No.32000041 and 32272789)+2 种基金the Shandong Provincial Natural Science Foundation,China(ZR2020QC005)the Qingdao Agricultural University Scientific Research Foundation(6631120076)horizontal project:Breeding and property protection of new varieties of factory produced Hypsizygus marmoreus(20183702012614).
文摘The wild Lepista sordida is a kind of precious and rare edible fungus.An excellent strain of it by artificial domestication was obtained,which was high-yield and high in iron content.In this study,high-throughput comparative proteomics was used to reveal the regulatory mechanism of its primordium differentiation in the early fruiting body formation.The mycelium before the primordium differentiation mainly expressed high levels of mitochondrial functional proteins and carbon dioxide concentration regulatory proteins.In young mushrooms,the highly expressed proteins were mainly involved in cell component generation,cell proliferation,nitrogen compound metabolism,nucleotide metabolism,glutathione metabolism,and purine metabolism.The differential regulation patterns of pileus and stipe growth to maturity were also revealed.The highly expressed proteins related to transcription,RNA splicing,the production of various organelles,DNA conformational change,nucleosome organization,protein processing,maturation and transport,and cell detoxification regulated the pileus development and maturity.The proteins related to carbohydrate and energy metabolism,large amounts of obsolete cytoplasmic parts,nutrient deprivation,and external stimuli regulated the stipe development and maturity.Multiple CAZymes regulated nutrient absorption,morphogenesis,spore production,stress response,and other life activities at different growth and development stages.
文摘采用纤维素酶(A)和中性蛋白酶(B)复合酶系水解香菇柄,确定香菇(Lentinus edodes)柄中风味成分最佳溶出条件。以可溶性蛋白质溶出率和氨基酸释放率为评价指标,分别考察酶A水解时料液比、p H、添加量、酶解温度及酶解时间和酶B水解时p H、添加量、酶解温度和酶解时间对提取率的影响;以游离氨基酸、呈味核苷酸、多糖的溶出率和酶解液感官品质为评价指标,比较了热水浸提法、纤维素酶单酶解法、复合酶解法3种方法的优劣。结果表明,最适酶解工艺条件为先以0.2%食用菌水解酶A,料液比(m∶V)为1∶6,p H 4.5,温度55℃的条件酶解2 h,在此条件下可溶性蛋白质溶出率为4.12%,然后在酶解液中加入0.15%酶B,于p H 6.5,温度50℃酶解6 h,在此条件下总氨基酸释放率为2.16%;复合酶法水解香菇柄所得游离氨基酸、呈味核苷酸、多糖含量、感官评分分别为2.16%、1.57%、7.25%、82分,均优于另外2种方法。
