In rice production,the prevention and management of pests and diseases have always received special attention.Traditional methods require human experts,which is costly and time-consuming.Due to the complexity of the s...In rice production,the prevention and management of pests and diseases have always received special attention.Traditional methods require human experts,which is costly and time-consuming.Due to the complexity of the structure of rice diseases and pests,quickly and reliably recognizing and locating them is difficult.Recently,deep learning technology has been employed to detect and identify rice diseases and pests.This paper introduces common publicly available datasets;summarizes the applications on rice diseases and pests from the aspects of image recognition,object detection,image segmentation,attention mechanism,and few-shot learning methods according to the network structure differences;and compares the performances of existing studies.Finally,the current issues and challenges are explored fromthe perspective of data acquisition,data processing,and application,providing possible solutions and suggestions.This study aims to review various DL models and provide improved insight into DL techniques and their cutting-edge progress in the prevention and management of rice diseases and pests.展开更多
[Objectives]The paper was to ascertain the prevalence of diseases and pests in a range of citrus nurseries situated in Guangdong Province and its neighboring provinces.[Methods]Citrus diseases and pests were systemati...[Objectives]The paper was to ascertain the prevalence of diseases and pests in a range of citrus nurseries situated in Guangdong Province and its neighboring provinces.[Methods]Citrus diseases and pests were systematically investigated,and citrus leaf samples were randomly collected from 15 citrus nurseries across 8 regions in Guangdong Province and its neighboring provinces.Quantitative polymerase chain reaction(qPCR)and reverse transcription polymerase chain reaction(RT-PCR)techniques were employed to detect diseases in the collected samples.Additionally,root and substrate samples were obtained,and root-knot nematodes were isolated using the Baermann funnel method.[Results]The positive detection rate of citrus huanglongbing(HLB)was recorded at 3%,indicating an increase in attention towards this disease compared to 2013.Additionally,the positive detection rate for citrus bacterial canker disease(CBCD)was found to be 16.5%.It was observed that the majority of nurseries with positive samples employed open field rearing practices without the use of mesh chambers,and the primary source of scions was self-propagation.The detection rate of citrus tristeza virus(CTV)was found to be the highest,with a positive detection rate of 63%,and the prevalence in disease-bearing nurseries reached as high as 90%.In comparison to 2013,there had been no improvement in the condition of seedlings affected by CTV.The positive detection rate of citrus yellow vein clearing virus(CYVCV)was found to be 38%,with 70%of the surveyed nurseries exhibiting the disease.The citrus varieties identified as carriers of the disease included‘Qicheng’,‘Shatangju’,‘Wogan’,and‘Gonggan’.Nematodes were isolated from the matrix and roots of seedlings grown in both container and open field environments.The susceptibility of container seedlings to nematodes was found to be 36.4%,while the susceptibility of open field seedlings was 38.6%.Statistical analysis indicated no significant difference in susceptibility between the two groups.[Conclusions]The disease detection rates associated with various seedling rearing methods and citrus varieties exhibited notable variability.Open field seedlings without the protection of mesh chambers demonstrated a higher susceptibility to disease.Additionally,the types of infectious diseases varied among the different citrus varieties.展开更多
[Objectives]This study was conducted to establish a rapid quantitative method for detecting antibody against Peste des Petits Ruminants Virus(PPR V)in sheep serum.[Methods]Soluble N protein and NH fusion protein were ...[Objectives]This study was conducted to establish a rapid quantitative method for detecting antibody against Peste des Petits Ruminants Virus(PPR V)in sheep serum.[Methods]Soluble N protein and NH fusion protein were obtained in Escherichia coli prokaryotic expression system by optimizing codons and expression conditions of E.coli.Furthermore,based on the purified soluble N protein and NH fusion protein,a high-sensitivity fluorescence immunoassay kit for detecting the antibody against PPR V was established.[Results]The method could quickly and quantitatively detect PPR V antibody in sheep serum,with high sensitivity and specificity,without any cross reaction to other related sheep pathogens.The intra-batch and inter-batch coefficients of variation were less than 10%and 15%,respectively,and the method had good repeatability.Through detection on 292 clinical serum samples,it was compared with the French IDVET competitive ELISA kit,and the coincidence rate of the two methods reached 93.84%.Compared with the serum neutralization test,the detected titer value of the high-sensitivity rapid fluorescence quantitative detection method was basically consistent with the tilter value obtained by the neutralization test on the standard positive serum(provided by the WOAH Brucellosis Reference Laboratory of France).[Conclusions]This method can realize rapid quantitative detection of PPR V antibody on site,and has high practical value and popularization value.展开更多
Image processing,agricultural production,andfield monitoring are essential studies in the researchfield.Plant diseases have an impact on agricultural production and quality.Agricultural disease detection at a preliminar...Image processing,agricultural production,andfield monitoring are essential studies in the researchfield.Plant diseases have an impact on agricultural production and quality.Agricultural disease detection at a preliminary phase reduces economic losses and improves the quality of crops.Manually identifying the agricultural pests is usually evident in plants;also,it takes more time and is an expensive technique.A drone system has been developed to gather photographs over enormous regions such as farm areas and plantations.An atmosphere generates vast amounts of data as it is monitored closely;the evaluation of this big data would increase the production of agricultural production.This paper aims to identify pests in mango trees such as hoppers,mealybugs,inflorescence midges,fruitflies,and stem borers.Because of the massive volumes of large-scale high-dimensional big data collected,it is necessary to reduce the dimensionality of the input for classify-ing images.The community-based cumulative algorithm was used to classify the pests in the existing system.The proposed method uses the Entropy-ELM method with Whale Optimization to improve the classification in detecting pests in agricul-ture.The Entropy-ELM method with the Whale Optimization Algorithm(WOA)is used for feature selection,enhancing mango pests’classification accuracy.Support Vector Machines(SVMs)are especially effective for classifying while users get var-ious classes in which they are interested.They are created as suitable classifiers to categorize any dataset in Big Data effectively.The proposed Entropy-ELM-WOA is more capable compared to the existing systems.展开更多
Pests detecting is an important research subject in grain storage field.In the past decades,many edge detection methods have been applied to the edge detection of stored grain pests.Although some of them can realize t...Pests detecting is an important research subject in grain storage field.In the past decades,many edge detection methods have been applied to the edge detection of stored grain pests.Although some of them can realize the stored grain pests detecting,precision and robustness are not good enough.Spectral residual(SR)saliency edge detection defines the logarithmic spectrumof image as novelty part of the image information.The remaining spectrumis converted to the airspace to obtain edge detection results.SR algorithm is completely based on frequency domain processing.It not only can effectively simplify the target detection algorithm,but also can improve the effectiveness of target recognition.The experimental results show that the edge results of stored grain pests detected by SR method are effective and stable.展开更多
A novel method for pest detection is proposed based on the theory of multi-fractal spectrum to extract pests on plant leaves.Both local and global information of the image regularity were obtained by multi-fractal ana...A novel method for pest detection is proposed based on the theory of multi-fractal spectrum to extract pests on plant leaves.Both local and global information of the image regularity were obtained by multi-fractal analysis.By applying fractal dimension,the spots on leaf images can be extracted without loosing or introducing any information.The different parts of images are re-analysis by morphology operations to determine the candidate regions of pests.The performance of multi-fractal analysis of whitefly detection is investigated through greenhouse experiments.The experimental results show that the proposed method is robust to noise from light and is not sensitive to the complex environment.展开更多
为提高自然环境下生姜叶片病虫害的识别精确率,提出一种基于改进YOLOv5s的生姜叶片病虫害识别模型。建立了田间不同自然环境条件下的生姜叶片病虫害数据集,为保证模型在田间移动设备上流畅运行,实现网络模型的轻量化,在YOLOv5s中引入Gho...为提高自然环境下生姜叶片病虫害的识别精确率,提出一种基于改进YOLOv5s的生姜叶片病虫害识别模型。建立了田间不同自然环境条件下的生姜叶片病虫害数据集,为保证模型在田间移动设备上流畅运行,实现网络模型的轻量化,在YOLOv5s中引入GhostNet网络中的Ghost模块和Ghost BottleNeck结构。同时,为避免生姜叶片病虫害图像小目标特征丢失的情况,增强图像特征提取,加入CA注意力机制模块,提升生姜叶片病虫害的识别准确率和定位精确度。改进后的模型参数量、计算量和权重文件大小分别为YOLOv5s模型的52.0%、50.6%和55.2%,对生姜叶片病虫害识别平均精度均值达到了83.8%。与Faster-RCNN、SSD、YOLOv4、YOLOv5s、Tea-YOLOv5s等算法相比,平均精度均值分别提高37.6、39.1、22.5、1.5、0.7个百分点,将改进后的目标检测模型部署在Jetson Orin NX开发板上,并使用TensorRT、Int8量化和CUDA等方法对检测模型加速,加速后的模型检测速度为74.3帧/s,满足实时检测的要求,测试结果显示,改进后的模型减少了漏检、误检的情况,并且对目标定位更加精准,适用于自然环境下生姜叶片病虫害的精准识别,为后续生姜机械自动化施药作业提供技术理论支持。展开更多
基金funded by Hunan Provincial Natural Science Foundation of China with Grant Numbers(2022JJ50016,2023JJ50096)Innovation Platform Open Fund of Hengyang Normal University Grant 2021HSKFJJ039Hengyang Science and Technology Plan Guiding Project with Number 202222025902.
文摘In rice production,the prevention and management of pests and diseases have always received special attention.Traditional methods require human experts,which is costly and time-consuming.Due to the complexity of the structure of rice diseases and pests,quickly and reliably recognizing and locating them is difficult.Recently,deep learning technology has been employed to detect and identify rice diseases and pests.This paper introduces common publicly available datasets;summarizes the applications on rice diseases and pests from the aspects of image recognition,object detection,image segmentation,attention mechanism,and few-shot learning methods according to the network structure differences;and compares the performances of existing studies.Finally,the current issues and challenges are explored fromthe perspective of data acquisition,data processing,and application,providing possible solutions and suggestions.This study aims to review various DL models and provide improved insight into DL techniques and their cutting-edge progress in the prevention and management of rice diseases and pests.
基金Supported by Earmarked Fund for China Agriculture Research System(CARS-26)Science and Technology Innovation Guidance Project of Zhaoqing City(2023040308008)+1 种基金Undergraduate Innovation and Entrepreneurship Training Program of Guangdong Province(S202310580050)Project of High-quality Development in Hundred Counties,Thousands Towns and Ten Thousand Villages.
文摘[Objectives]The paper was to ascertain the prevalence of diseases and pests in a range of citrus nurseries situated in Guangdong Province and its neighboring provinces.[Methods]Citrus diseases and pests were systematically investigated,and citrus leaf samples were randomly collected from 15 citrus nurseries across 8 regions in Guangdong Province and its neighboring provinces.Quantitative polymerase chain reaction(qPCR)and reverse transcription polymerase chain reaction(RT-PCR)techniques were employed to detect diseases in the collected samples.Additionally,root and substrate samples were obtained,and root-knot nematodes were isolated using the Baermann funnel method.[Results]The positive detection rate of citrus huanglongbing(HLB)was recorded at 3%,indicating an increase in attention towards this disease compared to 2013.Additionally,the positive detection rate for citrus bacterial canker disease(CBCD)was found to be 16.5%.It was observed that the majority of nurseries with positive samples employed open field rearing practices without the use of mesh chambers,and the primary source of scions was self-propagation.The detection rate of citrus tristeza virus(CTV)was found to be the highest,with a positive detection rate of 63%,and the prevalence in disease-bearing nurseries reached as high as 90%.In comparison to 2013,there had been no improvement in the condition of seedlings affected by CTV.The positive detection rate of citrus yellow vein clearing virus(CYVCV)was found to be 38%,with 70%of the surveyed nurseries exhibiting the disease.The citrus varieties identified as carriers of the disease included‘Qicheng’,‘Shatangju’,‘Wogan’,and‘Gonggan’.Nematodes were isolated from the matrix and roots of seedlings grown in both container and open field environments.The susceptibility of container seedlings to nematodes was found to be 36.4%,while the susceptibility of open field seedlings was 38.6%.Statistical analysis indicated no significant difference in susceptibility between the two groups.[Conclusions]The disease detection rates associated with various seedling rearing methods and citrus varieties exhibited notable variability.Open field seedlings without the protection of mesh chambers demonstrated a higher susceptibility to disease.Additionally,the types of infectious diseases varied among the different citrus varieties.
基金Supported by The National Project for the Prevention and Control of Major Exotic Animal Diseases(2022YFD1800500)National Mutton Sheep Industrial Technology System(CARS39).
文摘[Objectives]This study was conducted to establish a rapid quantitative method for detecting antibody against Peste des Petits Ruminants Virus(PPR V)in sheep serum.[Methods]Soluble N protein and NH fusion protein were obtained in Escherichia coli prokaryotic expression system by optimizing codons and expression conditions of E.coli.Furthermore,based on the purified soluble N protein and NH fusion protein,a high-sensitivity fluorescence immunoassay kit for detecting the antibody against PPR V was established.[Results]The method could quickly and quantitatively detect PPR V antibody in sheep serum,with high sensitivity and specificity,without any cross reaction to other related sheep pathogens.The intra-batch and inter-batch coefficients of variation were less than 10%and 15%,respectively,and the method had good repeatability.Through detection on 292 clinical serum samples,it was compared with the French IDVET competitive ELISA kit,and the coincidence rate of the two methods reached 93.84%.Compared with the serum neutralization test,the detected titer value of the high-sensitivity rapid fluorescence quantitative detection method was basically consistent with the tilter value obtained by the neutralization test on the standard positive serum(provided by the WOAH Brucellosis Reference Laboratory of France).[Conclusions]This method can realize rapid quantitative detection of PPR V antibody on site,and has high practical value and popularization value.
文摘Image processing,agricultural production,andfield monitoring are essential studies in the researchfield.Plant diseases have an impact on agricultural production and quality.Agricultural disease detection at a preliminary phase reduces economic losses and improves the quality of crops.Manually identifying the agricultural pests is usually evident in plants;also,it takes more time and is an expensive technique.A drone system has been developed to gather photographs over enormous regions such as farm areas and plantations.An atmosphere generates vast amounts of data as it is monitored closely;the evaluation of this big data would increase the production of agricultural production.This paper aims to identify pests in mango trees such as hoppers,mealybugs,inflorescence midges,fruitflies,and stem borers.Because of the massive volumes of large-scale high-dimensional big data collected,it is necessary to reduce the dimensionality of the input for classify-ing images.The community-based cumulative algorithm was used to classify the pests in the existing system.The proposed method uses the Entropy-ELM method with Whale Optimization to improve the classification in detecting pests in agricul-ture.The Entropy-ELM method with the Whale Optimization Algorithm(WOA)is used for feature selection,enhancing mango pests’classification accuracy.Support Vector Machines(SVMs)are especially effective for classifying while users get var-ious classes in which they are interested.They are created as suitable classifiers to categorize any dataset in Big Data effectively.The proposed Entropy-ELM-WOA is more capable compared to the existing systems.
基金financially supported by National Natural Science Foundation of China(No.61871176)Key Scientific and Technological Project of Science and Technology Department of Henan Province(No.172102210030,182102110099)+2 种基金Key Scientific Research Project Program of Universities of Henan Province(No.18B520025)Open Fund of Key Laboratory of Grain Information Processing and Control(No.KFJJ-2018-102)supported by Collaborative Innovation Center of Grain Storage and Security of Henan Province
文摘Pests detecting is an important research subject in grain storage field.In the past decades,many edge detection methods have been applied to the edge detection of stored grain pests.Although some of them can realize the stored grain pests detecting,precision and robustness are not good enough.Spectral residual(SR)saliency edge detection defines the logarithmic spectrumof image as novelty part of the image information.The remaining spectrumis converted to the airspace to obtain edge detection results.SR algorithm is completely based on frequency domain processing.It not only can effectively simplify the target detection algorithm,but also can improve the effectiveness of target recognition.The experimental results show that the edge results of stored grain pests detected by SR method are effective and stable.
基金supported by the MKE(The Ministry of Knowledge Economy),Korea,under the ITRC(Infor mationTechnology Research Center)support programsupervised by the NIPA(National IT Industry Promotion Agency)(NIPA-2010-C1090-1021-0010)
文摘A novel method for pest detection is proposed based on the theory of multi-fractal spectrum to extract pests on plant leaves.Both local and global information of the image regularity were obtained by multi-fractal analysis.By applying fractal dimension,the spots on leaf images can be extracted without loosing or introducing any information.The different parts of images are re-analysis by morphology operations to determine the candidate regions of pests.The performance of multi-fractal analysis of whitefly detection is investigated through greenhouse experiments.The experimental results show that the proposed method is robust to noise from light and is not sensitive to the complex environment.
文摘为提高自然环境下生姜叶片病虫害的识别精确率,提出一种基于改进YOLOv5s的生姜叶片病虫害识别模型。建立了田间不同自然环境条件下的生姜叶片病虫害数据集,为保证模型在田间移动设备上流畅运行,实现网络模型的轻量化,在YOLOv5s中引入GhostNet网络中的Ghost模块和Ghost BottleNeck结构。同时,为避免生姜叶片病虫害图像小目标特征丢失的情况,增强图像特征提取,加入CA注意力机制模块,提升生姜叶片病虫害的识别准确率和定位精确度。改进后的模型参数量、计算量和权重文件大小分别为YOLOv5s模型的52.0%、50.6%和55.2%,对生姜叶片病虫害识别平均精度均值达到了83.8%。与Faster-RCNN、SSD、YOLOv4、YOLOv5s、Tea-YOLOv5s等算法相比,平均精度均值分别提高37.6、39.1、22.5、1.5、0.7个百分点,将改进后的目标检测模型部署在Jetson Orin NX开发板上,并使用TensorRT、Int8量化和CUDA等方法对检测模型加速,加速后的模型检测速度为74.3帧/s,满足实时检测的要求,测试结果显示,改进后的模型减少了漏检、误检的情况,并且对目标定位更加精准,适用于自然环境下生姜叶片病虫害的精准识别,为后续生姜机械自动化施药作业提供技术理论支持。