The interaction mechanism between coal and rock masses with supporting materials is significant in roadway control, especially in deep underground mining situations where dynamic hazards frequently happened due to hig...The interaction mechanism between coal and rock masses with supporting materials is significant in roadway control, especially in deep underground mining situations where dynamic hazards frequently happened due to high geo-stress and strong disturbed effects. This paper is to investigate the strain energy evolution in the interaction between coal and rock masses with self-designed energy-absorbing props and rock bolts by numerical modeling with the finite difference method. The interaction between rock and rock bolt/prop is accomplished by the cables element and the interface between the inner and outer props. Roadway excavation and coal extraction conditions in deep mining are numerically employed to investigate deformation, plastic zone ranges, strain energy input, accumulation, dissipation,and release. The effect on strain energy input, accumulation, dissipation, and release with rock deformation, and the plastic zone is addressed. A ratio of strain energy accumulation, dissipation, and release with energy input a, β, γ is to assess the dynamic hazards. The effects on roadway excavation and coal extraction steps of a, β, γ are discussed. The results show that:(1) In deep high geo-stress roadways, the energyabsorbing support system plays a dual role in resisting deformation and reducing the scope of plastic zones in surrounding rock, as well as absorbing energy release in the surrounding rock, especially in the coal extraction state to mitigate disturbed effects.(2) The strain energy input, accumulation is dependent on roadway deformation, the strain energy dissipation is relied on plastic zone area and disturbed effects, and strain energy release density is the difference among the three. The function of energyabsorbing rock bolts and props play a key role to mitigate strain energy release density and amount, especially in coal extraction condition, with a peak density value from 4×10^(4) to 1×10^(4)J/m^(3), and amount value from 3.57×10^(8) to 1.90×10^(6)J.(3) When mining is advanced in small steps, the strain energy accumulation is dominated. While in a large step, the released energy is dominant, thus a more dynamic hazards proneness. The energy-absorbing rock bolt and prop can reduce three times strain energy release amount, thus reducing the dynamic hazards. The results suggest that energy-absorbing props and rock bolts can effectively reduce the strain energy in the coal and rock masses, and prevent rock bursts and other hazards.The numerical model developed in this study can also be used to optimize the design of energyabsorbing props and rock bolts for specific mining conditions.展开更多
An approach for estimating ground surface rupture caused by strong earthquakes is presented in this paper, where the finite element (FE) method of continuous and discontinuous coalescent displacement fields is adopt...An approach for estimating ground surface rupture caused by strong earthquakes is presented in this paper, where the finite element (FE) method of continuous and discontinuous coalescent displacement fields is adopted. The onset condition of strain localization is introduced to detect the formation of the slippage line. In the analysis, the Drucker-Prager constitutive model is used for soils and the rate- and state-dependent friction law is used on the slippage line to simulate the evolution of the sliding. A simple application to evaluate the ground surface rupture induced by a reverse fault movement is provided, and the numerical simulation shows good agreement with failure characteristics observed in the field after strong earthquakes.展开更多
The engineering of microbial cell factories for the production of high-value chemicals from renewable resources presents several challenges,including the optimization of key enzymes,pathway fluxes and metabolic networ...The engineering of microbial cell factories for the production of high-value chemicals from renewable resources presents several challenges,including the optimization of key enzymes,pathway fluxes and metabolic networks.Addressing these challenges involves the development of synthetic auxotrophs,a strategy that links cell growth with enzyme properties or biosynthetic pathways.This linkage allows for the improvement of enzyme properties by in vivo directed enzyme evolution,the enhancement of metabolic pathway fluxes under growth pressure,and remodeling of metabolic networks through directed strain evolution.The advantage of employing synthetic auxotrophs lies in the power of growth-coupled selection,which is not only high-throughput but also labor-saving,greatly simplifying the development of both strains and enzymes.Synthetic auxotrophs play a pivotal role in advancing microbial cell factories,offering benefits from enzyme optimization to the manipulation of metabolic networks within single microbes.Furthermore,this strategy extends to coculture systems,enabling collaboration within microbial communities.This review highlights the recently developed applications of synthetic auxotrophs as microbial cell factories,and discusses future perspectives,aiming to provide a practical guide for growth-coupled models to produce value-added chemicals as part of a sustainable biorefinery.展开更多
基金the National Natural Science Foundation of China(Nos.52204114,52274145,U22A20165,and 52174089)the Natural Science Foundation of Jiangsu Province(No.BK20210522)+2 种基金the National Key Research and Development Program of China(No.2022YFE0128300)the China Postdoctoral Science Foundation(No.2023M733758)the Shandong Postdoctoral Science Foundation(No.SDCX-ZG-202302037).
文摘The interaction mechanism between coal and rock masses with supporting materials is significant in roadway control, especially in deep underground mining situations where dynamic hazards frequently happened due to high geo-stress and strong disturbed effects. This paper is to investigate the strain energy evolution in the interaction between coal and rock masses with self-designed energy-absorbing props and rock bolts by numerical modeling with the finite difference method. The interaction between rock and rock bolt/prop is accomplished by the cables element and the interface between the inner and outer props. Roadway excavation and coal extraction conditions in deep mining are numerically employed to investigate deformation, plastic zone ranges, strain energy input, accumulation, dissipation,and release. The effect on strain energy input, accumulation, dissipation, and release with rock deformation, and the plastic zone is addressed. A ratio of strain energy accumulation, dissipation, and release with energy input a, β, γ is to assess the dynamic hazards. The effects on roadway excavation and coal extraction steps of a, β, γ are discussed. The results show that:(1) In deep high geo-stress roadways, the energyabsorbing support system plays a dual role in resisting deformation and reducing the scope of plastic zones in surrounding rock, as well as absorbing energy release in the surrounding rock, especially in the coal extraction state to mitigate disturbed effects.(2) The strain energy input, accumulation is dependent on roadway deformation, the strain energy dissipation is relied on plastic zone area and disturbed effects, and strain energy release density is the difference among the three. The function of energyabsorbing rock bolts and props play a key role to mitigate strain energy release density and amount, especially in coal extraction condition, with a peak density value from 4×10^(4) to 1×10^(4)J/m^(3), and amount value from 3.57×10^(8) to 1.90×10^(6)J.(3) When mining is advanced in small steps, the strain energy accumulation is dominated. While in a large step, the released energy is dominant, thus a more dynamic hazards proneness. The energy-absorbing rock bolt and prop can reduce three times strain energy release amount, thus reducing the dynamic hazards. The results suggest that energy-absorbing props and rock bolts can effectively reduce the strain energy in the coal and rock masses, and prevent rock bursts and other hazards.The numerical model developed in this study can also be used to optimize the design of energyabsorbing props and rock bolts for specific mining conditions.
基金National Science Foundation Council State KeyLaboratory of Frozen Soil Engineering (SKLFSE200504)State Commonweal Research Project (2002DIB30076)
文摘An approach for estimating ground surface rupture caused by strong earthquakes is presented in this paper, where the finite element (FE) method of continuous and discontinuous coalescent displacement fields is adopted. The onset condition of strain localization is introduced to detect the formation of the slippage line. In the analysis, the Drucker-Prager constitutive model is used for soils and the rate- and state-dependent friction law is used on the slippage line to simulate the evolution of the sliding. A simple application to evaluate the ground surface rupture induced by a reverse fault movement is provided, and the numerical simulation shows good agreement with failure characteristics observed in the field after strong earthquakes.
基金supported by the National Key R&D Program of China(Grant No.2022YFC2106100)the National Natural Science Foundation of China(Grant Nos.22078011,22378016,and 22238001)Guangdong Key Area Research and Development Program(Grant No.2022B1111080003).
文摘The engineering of microbial cell factories for the production of high-value chemicals from renewable resources presents several challenges,including the optimization of key enzymes,pathway fluxes and metabolic networks.Addressing these challenges involves the development of synthetic auxotrophs,a strategy that links cell growth with enzyme properties or biosynthetic pathways.This linkage allows for the improvement of enzyme properties by in vivo directed enzyme evolution,the enhancement of metabolic pathway fluxes under growth pressure,and remodeling of metabolic networks through directed strain evolution.The advantage of employing synthetic auxotrophs lies in the power of growth-coupled selection,which is not only high-throughput but also labor-saving,greatly simplifying the development of both strains and enzymes.Synthetic auxotrophs play a pivotal role in advancing microbial cell factories,offering benefits from enzyme optimization to the manipulation of metabolic networks within single microbes.Furthermore,this strategy extends to coculture systems,enabling collaboration within microbial communities.This review highlights the recently developed applications of synthetic auxotrophs as microbial cell factories,and discusses future perspectives,aiming to provide a practical guide for growth-coupled models to produce value-added chemicals as part of a sustainable biorefinery.