Neutrophil extracellular traps mediate neuro-immunothrombosis

Neutrophil extracellular traps are primarily composed of DNA and histones and are released by neutrophils to promote inflammation and thrombosis when stimulated by various inflammato ry reactions.Neutrophil extracellular trap formation occurs through lytic and non-lytic pathways that can be further classified by formation mechanisms.Histones,von Willebrand factor,fibrin,and many other factors participate in the interplay between inflammation and thrombosis.Neuroimmunothrombosis summarizes the intricate interplay between inflammation and thrombosis during neural development and the pathogenesis of neurological diseases,providing cutting-edge insights into post-neurotrauma thrombotic events.The blood-brain barrier defends the brain and spinal cord against external assaults,and neutrophil extracellular trap involvement in blood-brain barrier disruption and immunothrombosis contributes substantially to secondary injuries in neurological diseases.Further research is needed to understand how neutrophil extracellular traps promote blood-brain barrier disruption and immunothrombosis,but recent studies have demonstrated that neutrophil extracellular traps play a crucial role in immunothrombosis,and identified modulators of neuro-immunothrombosis.However,these neurological diseases occur in blood vessels,and the mechanisms are unclear by which neutrophil extracellular traps penetrate the blood-brain barrier to participate in immunothrombosis in traumatic brain injury.This review discusses the role of neutrophil extracellular traps in neuro-immunothrombosis and explores potential therapeutic interventions to modulate neutrophil extracellular traps that may reduce immunothrombosis and improve traumatic brain injury outcomes.

Crossroads:Pathogenic role and therapeutic targets of neutrophil extracellular traps in rheumatoid arthritis

Rheumatoid arthritis(RA)is a prevalent autoimmune disease whose main features include chronic synovial inflammation,bone destruction,and joint degeneration.Neutrophils are often considered to be the first responders to inflammation and are a key presence in the inflammatory milieu of RA.Neutrophil extracellular traps(NETs),a meshwork of DNA-histone complexes and proteins released by activated neutrophils,are widely involved in the pathophysiology of autoimmune diseases,especially RA,in addition to playing a key role in the neutrophil innate immune response.NETs have been found to be an important source of citrullinated autoantigen antibodies and inflammatory factor release,which can activate RA synovial fibroblasts(FLS)and cause joint damage.This article reviews the role of NETs in the pathophysiology of RA,demonstrating the application of multiple molecules with various therapies,with a view to informing the discovery and development of novel biomarkers and therapeutic targets for RA.

Compact magneto-optical traps using planar optics

Magneto-optical traps (MOTs) composed of magnetic fields and light fields have been widely utilized to cool andconfine microscopic particles. Practical technology applications require miniaturized MOTs. The advancement of planaroptics has promoted the development of compact MOTs. In this article, we review the development of compact MOTs basedon planar optics. First, we introduce the standardMOTs. We then introduce the gratingMOTs with micron structures, whichhave been used to build cold atomic clocks, cold atomic interferometers, and ultra-cold sources. Further, we introducethe integrated MOTs based on nano-scale metasurfaces. These new compact MOTs greatly reduce volume and powerconsumption, and provide new opportunities for fundamental research and practical applications.

New Forward Gated-Diode Technique for Separating Front Gate Interface- from Oxide-Traps Induced by Hot-Carrier-Stress in SOI-NMOSFETs

The front gate interface and oxide traps induced by hot carrier stress in SOI NMOSFETs are studied.Based on a new forward gated diode technique,the R G current originating from the front interface traps is measured,and then the densities of the interface and oxide traps are separated independently.The experimental results show that the hot carrier stress of front channel not only results in the strong generation of the front interface traps,but also in the significant oxide traps.These two kinds of traps have similar characteristic in increasing with the hot carrier stress time.This analysis allows one to obtain a clear physical picture of the effects of the hot carrier stress on the generating of interface and oxide traps,which help to understand the degradation and reliability of the SOI MOSFETs.

Carbon-Induced Deep Traps Responsible for Current Collapse in AlGaN/GaN HEMTs

Although outstanding microwave power performance of AlGaN/GaN HEMTs has been reported,drain current collapse is still a problem. In this paper,an experiment was carried out to demonstrate one factor causing the collapse. Two AlGaN/GaN samples were annealed under N2-atmosphere with and without carbon incorporation, and the XPS measurement technique was used to determine that the concentration of carbon impurity in the latter sample was far higher than in the former. From the comparison of two Id- Vds characteristics,we conclude that carbon impurity incorporation is responsible for the severe current collapse. The carbon impurity-induced deep traps under negative gate bias stress can capture the channel carriers, which release slowly from these traps under positive bias stress,thus causing the current collapse.

Dependence of R-G Currenton Bulk Traps Characteristics and Silicon Film Structure in SOI Gated-Diode

The dependence of the Recombination- Generation( R- G) current on the bulk trap characteristics and sili- con film structure in SOI lateral p+ p- n+ diode has been analyzed num erically by using the simulation tool,DESSIS- ISE.By varying the bulk trap characteristics such as the trap density and energy level spectrum systematically,the dependence of the R- G current on both of them has been dem onstrated in details.Moreover,the silicon film doping concentration and thickness are changed to make silicon body varies from the fully- depletion m ode into the partial- ly- depletion one.The influence of the transfer of silicon body characteristics on the R- G currenthas also been care- fully examined.A better understanding is obtained of the behavior of bulk trap R- G current in the SOI lateral gat- ed- diode.

BG-Trap法与双层叠帐法对登革热媒介白纹伊蚊监测效果的比较

目的比较BG-trap法与双层叠帐法对登革热媒介白纹伊蚊的监测效果,进一步探讨BG-trap法用于登革热媒介伊蚊监测的可能性,为制定更有效的登革热防控策略提供参考依据。方法实验选取江苏省3个设区市的6个户外监测点,于8—9月的3天时间中,分别于每日16:00-16:30、17:00-17:30、18:00-18:30三个时段同步开展BG-Trap法和双层叠帐法监测。结果BG-Trap法和双层叠帐法均能够有效地捕获白纹伊蚊的雌蚊,双层叠账法捕获的白纹伊蚊占比高于BG-Trap法(χ^(2)=16.034,P<0.01),两种方法捕获的白纹伊蚊雌蚊构成比之间差异无统计学意义(χ^(2)=0.162,P=0.687)。BG-Trap法捕获的白纹伊蚊平均密度指数为7.85±5.23只/(台·h),低于双层叠帐法的27.04±13.86只/(顶·h),差异有统计学意义(t=6.73,P<0.001)。结论BG-Trap法对登革热媒介白纹伊蚊监测效果不及双层叠帐法,其能否应用于登革热媒介伊蚊监测有待于进一步研究。

BG-Trap法在伊蚊成蚊监测中的应用研究

目的采用BG-Trap法进行伊蚊监测,探讨该方法应用于登革热蚊媒常规监测和应急监测的可行性。方法在伊蚊监测场所用BG-Trap法分时段进行监测,用非参数统计分析的Chi-Square过程和t检验对监测结果进行统计和分析。结果应用BG-Trap法对伊蚊进行监测,15:00—17:00监测效果明显高于9:00—11:00(雌:χ^(2)=18963,df=1,P<0001;雄:χ^(2)=18667,df=1,P<0001;总体:χ^(2)=37500,df=1,P<0001);从12:00开始,按每小时一段统计诱蚊效果,以15:00—19:00时段对伊蚊引诱效果最好,平均捕蚊量为(618±21)只/h,平均小时诱蚊量与12:00—15:00有极显著的差异(雌:t=275,df=146,P<0001;雄:t=678,df=156,P<0001;总体:t=1016,df=304,P<0001)。结论可将BG-Trap法引入伊蚊的常规监测和应急监测。

Do neutrophil extracellular traps contribute to the heightened risk of thrombosis in inflammatory diseases?

Thrombotic events,both arterial and venous,are a major health concern worldwide. Further,autoimmune diseases,such as systemic lupus erythematosus,anti-neutrophil cytoplasmic antibody(ANCA)-associated vasculitis,and antiphospholipid syndrome,predispose to thrombosis,and thereby push the risk for these morbid events even higher. In recent years,neutrophils have been identified as important players in both arterial and venous thrombosis. Specifically,chromatin-based structures called neutrophil extracellular traps(NETs) play a key role in activating the coagulation cascade,recruiting platelets,and serving as scaffolding upon which the thrombus can be assembled. At the same time,neutrophils and NETs are emerging as important mediators of pathogenic inflammation in the aforementioned autoimmune diseases. Here,we first review the general role of NETs in thrombosis. We then posit that exaggerated NET release contributes to the prothrombotic diatheses of systemic lupus erythematosus,ANCA-associated vasculitis,and antiphospholipid syndrome.

2D study of AlGaN/AlN/GaN/AlGaN HEMTs' response to traps

In this work, the effects of GaN channel traps and temperature on the performance of AlGaN/AlN/GaN/AlGaN high electron mobility transistors(HEMTs) on Si(111) substrate, were investigated. 2 D simulations carried out using the Silvaco TCAD simulator tool for different drain and gate voltages showed that acceptor-like traps in the channel have a significant influence on the DC and RF characteristics. It was found that deeper acceptors below the conduction band with larger concentration have a more pronounced effect on the transistor performance. Meanwhile, the donor-like traps show no influence. Pulsing the device with different pulse widths and bias conditions, as well as increasing temperature, showed that the traps are more ionized when the pulse is wider or the temperature is higher, which can degrade the drain current and thus the DC characteristics of the transistor. Passivation of the transistor has also a beneficial effect on performance.

Genetic models of structural traps related to normal faults in the Putaohua Oilfield,Songliao Basin

The Putaohua Oilfield is a fault-prolific area and the faults have close relation with structural traps. The genetic models of the structural traps in the Putaohua Oilfield can be divided into two types： individual fault model and multi-fault interaction model. This is based on the description of displacement distribution of typical individual normal faults, the geometry of the footwall and hanging wall, and the analysis of the interaction between faults and the corresponding change in geometry when the faults grow. The individual fault model is that the displacement reaches a maximum at or near the center of fault and decreases toward the fault tips, so a half-graben is formed on the hanging wall of the fault and a half- anticline is formed on the footwall because of the isostatic process. The multi-fault interaction model is that during the growth of faults, they overlap and interact with each other, and accommodation zones are formed in the overlapping segments. The accommodation zones are favorable targets for hydrocarbon exploration, and the trap characteristics are dependent The multi-fault interaction model can be subdivided on the extent of overlap and occurrence of faults. into three types： synthetic accommodation zone, convergent accommodation zone and divergent accommodation zone. Hydrocarbon migration and accumulation models of each type have been developed. The hydrocarbon migration and accumulation models of the traps with different genetic models have their own characteristics in the different stages of fault growth.

Performance characteristics of tunnel boring machine in basalt and pyroclastic rocks of Deccan traps–A case study

A12.24km long tunnel between Maroshi and Ruparel College is being excavated by tunnel boring machine(TBM)to improve the water supply system of Greater Mumbai,India.In this paper,attempt has been made to establish the relationship between various litho-units of Deccan traps,stability of tunnel and TBM performances during the construction of5.83km long tunnel between Maroshi and Vakola.The Maroshi–Vakola tunnel passes under the Mumbai Airport and crosses both runways with an overburden cover of around70m.The tunneling work was carried out without disturbance to the ground.The rock types encountered during excavation arefine compacted basalt,porphyritic basalt,amygdaloidal basalt pyroclastic rocks with layers of red boles and intertrappean beds consisting of various types of shales Relations between rock mass properties,physico-mechanical properties,TBM specifications and the cor responding TBM performance were established.A number of support systems installed in the tunne during excavation were also discussed.The aim of this paper is to establish,with appropriate accuracy the nature of subsurface rock mass condition and to study how it will react to or behave during under ground excavation by TBM.The experiences gained from this project will increase the ability to cope with unexpected ground conditions during tunneling using TBM.

Structural Traps in Detachment Folds:a Case Study from the 'Comband Trough-like' Deformation Zone/s,East Sichuan,China

Detachment structures occur widely in the crust, and it is the commonest and most important deformation type developed in the region between orogenic belts and basins. The ＇comb-like＇ and ＇toughlike＇ fold belts in eastern Sichuan are caused by multi-layer detachment. The duplex structure is the most important deformation style in the region, exhibiting different characteristics from typical detachment structures. Different deformation styles, scales, and shortenings resulting from independent deformations of various detachment systems would lead to the phenomenon whereby most of the topographical heights in the region do not correspond to the structural heights in depth. Based on systematic structural analysis and combined with practical oil/gas prospecting, four types of structural traps are described from eastern Sichuan Province, which are： detachment and thrust trap; detachment folding trap; fault-flat blocking trap; and detachment layer trap. Meticulous studies on the deformation and distribution of detachment layers in the eastern Sichuan Province will contribute to oil/gas prospecting and selection of potential regions of marine-origin oil/gas prospecting in South China.

基于UPLC-Q-TRAP-MS分析养心安神药对酸枣仁-五味子配伍煎煮前后10个指标成分含量变化

目的分析养心安神药对酸枣仁-五味子配伍煎煮前后主要指标成分差异变化,并建立含量测定方法。方法采用Welch Ultimate XB-C_(18)色谱柱(2.1 mm×50 mm,5μm),以0.1%甲酸水溶液(A)-乙腈(B)为流动相,流速0.4 mL/min柱温40℃,梯度洗脱;质谱采用电喷雾离子源(ESI),多反应监测离子扫描模式(MRM)。分别对该药对单煎液、单煎合并液与合煎液中主要指标成分酸枣仁皂苷A、斯皮诺素、五味子醇甲、五味子酯甲、五味子甲素、五味子乙素、芦丁、槲皮素、山柰酚、山柰酚-3-O-云香糖苷进行含量测定,并分析药对配伍前后主要指标成分的含量变化差异。结果以上10种成分在一定浓度范围内均呈现良好的线性关系;精密度、重复性和稳定性RSD均良好;平均加样回收率在95.0%~102.1%,RSD为0.03%~3.7%。本次实验结果与前期试验认为酸枣仁与五味子在合煎后,主要指标成分降低保持一致性。结论多指标成分同时测定为该药对配伍质量全面控制提供新的方法并为其药效物质基础的深入研究提供依据。

Neutrophil extracellular traps mediate the crosstalk between glioma progression and the tumor microenvironment via the HMGB1/RAGE/IL-8 axis

Objective:Neutrophil extracellular traps(NETs)produced by tumor-infiltrating neutrophils(TINs)are associated with poor prognosis in patients with several types of cancer.However,the mechanisms underlying the involvement of NETs in glioma progression remain largely unknown.This study aimed to elucidate the roles of NETs in biological processes that drive the crosstalk between glioma progression and the tumor microenvironment.Methods:Neutrophil infiltration and NETs formation were investigated in glioma tissue through immunohistochemistry,and their relationships with clinicopathological features and outcomes were statistically evaluated.The effects of NETs on glioma cell progression were studied in a co-culture system.In vivo and in vitro experiments validated the reactive oxygen species activity and cytokine production of TINs,as well as the ERK signaling pathway activation and the metastasis of gliomas.Results:Neutrophil infiltration and NETs formation were induced in high-grade glioma compared with low-grade glioma.NETs induced by TINs were determined to be an oncogenic marker of high-grade gliomas and to be involved in cell proliferation and invasion.NETs overproduction promoted glioma cell proliferation,migration,and invasion.Furthermore,HMGB1 was found to bind to RAGE and activate the NF-κB signaling pathway in vitro.In addition,NETs stimulated the NF-κB signaling pathway,thus promoting IL-8 secretion in glioblastoma.Subsequently,IL-8 recruited neutrophils which in turn mediated NETs formation via the PI3 K/AKT/ROS axis in TINs.Conclusions:Our results suggest that NETs produced by TINs mediate the crosstalk between glioma progression and the tumor microenvironment by regulating the HMGB1/RAGE/IL-8 axis.Targeting NETs formation or IL-8 secretion may be an effective approach to inhibit glioma progression.

Distribution and controls of petroliferous plays in subtle traps within a Paleogene lacustrine sequence stratigraphic framework, Dongying Depression, Bohai Bay Basin, Eastern China

The characteristics of petroliferous plays in subtle traps within a sequence stratigraphic framework in the Dongying Depression are investigated in this study.Sand bodies within lowstand systems tracts(LSTs)of sequences,comprising incisedchannel fills,sublacustrine fans,deltas in LSTs,controlled by syndepositional normal faults,and sand bodies within transgressive systems tracts(TSTs)to early highstand systems tracts(HSTs),consisting of beach bars,and turbidites,controlled by the prodelta slope,paleorelief,and syndepositional normal faults,are good subtle reservoirs.Mudstones and shale of deep lake subfacies in TSTs to early HSTs of sequences are source and cap rocks.Abnormal overpressure is the dominant dynamic factor for hydrocarbon migration from source rock to the subtle traps.Normal faults,sand bodies,and unconformities function as conduit systems.Sand bodies distributed in the abnormal overpressure source rocks within LSTs to early HSTs are petroliferous plays in lithologic traps.The petroliferous plays in stratigraphic traps are controlled by unconformities at margins of the Depression.

High-mobility SiC MOSFET with low density of interface traps using high pressure microwave plasma oxidation

The microwave plasma oxidation under the relatively high pressure(6 kPa)region is introduced into the fabrication process of SiO2/4 H-SiC stack.By controlling the oxidation pressure,species,and temperature,the record low density of interface traps(~4×10^(10)cm^(-2)·eV^(-1)@Ec-0.2 eV)is demonstrated on SiO2/SiC stack formed by microwave plasma oxidation.And high quality SiO2 with very flat interface(0.27-nm root-mean-square roughness)is obtained.High performance Si C metal–oxide–semiconductor field-effect transistors(MOSFETs)with peak field effect mobility of 44 cm^(-2)·eV^(-1)is realized without additional treatment.These results show the potential of a high-pressure plasma oxidation step for improving the channel mobility in SiC MOSFETs.

Neutrophil extracellular traps participate in the development of cancer-associated thrombosis in patients with gastric cancer

BACKGROUND The development of venous thromboembolism(VTE) is associated with high mortality among gastric cancer(GC) patients. Neutrophil extracellular traps(NETs) have been reported to correlate with the prothrombotic state in some diseases, but are rarely reported in GC patients.AIM To investigate the effect of NETs on the development of cancer-associated thrombosis in GC patients.METHODS The levels of NETs in blood and tissue samples of patients were analyzed by ELISA, flow cytometry, and immunofluorescence staining. NET generation and hypercoagulation of platelets and endothelial cells(ECs) in vitro were observed by immunofluorescence staining. NET procoagulant activity(PCA) was determined by fibrin formation and thrombin–antithrombin complex(TAT) assays.Thrombosis in vivo was measured in a murine model induced by flow stenosis in the inferior vena cava(IVC).RESULTS NETs were likely to form in blood and tissue samples of GC patients compared with healthy individuals. In vitro studies showed that GC cells and their conditioned medium, but not gastric mucosal epithelial cells, stimulated NET release from neutrophils. In addition, NETs induced a hypercoagulable state of platelets by upregulating the expression of phosphatidylserine and Pselectin on the cells. Furthermore, NETs stimulated the adhesion of normal platelets on glass surfaces. Similarly, NETs triggered the conversion of ECs to hypercoagulable phenotypes by downregulating the expression of their intercellular tight junctions but upregulating that of tissue factor. Treatment of normal platelets or ECs with NETs augmented the level of plasma fibrin formation and the TAT complex. In the models of IVC stenosis, tumor-bearing mice showed a stronger ability to form thrombi, and NETs abundantly accumulated in the thrombi of tumorbearing mice compared with control mice. Notably, the combination of deoxyribonuclease I,activated protein C, and sivelestat markedly abolished the PCA of NETs.CONCLUSION GC-induced NETs strongly increased the risk of VTE development both in vitro and in vivo. NETs are potential therapeutic targets in the prevention and treatment of VTE in GC patients.

Surface traps-related nonvolatile resistive switching memory effect in a single SnO2:Sm nanowire

For nanostructure SnO2,it is very difficult for its electric properties to accurately control due to the presence of abundant surface states.The introduction of Sm can improve the traps in surface space charge region of SnO2 nanowires,resulting in a controllable storage charge effect.For the single nanowire-based two-terminal device,two surface state-related back-to-back diodes are formed.At a relatively large voltage,electrons can be injected into the traps in surface space charge region from negative electrode,resulting in a decrease of surface barrier connected with negative electrode,and contrarily electrons can be extracted from the traps in surface space charge region into positive electrode,resulting in an increase of surface barrier connected with positive electrode.The reversible injection and extraction induce a nonvolatile resistive switching memory effect.

Tectonic significance of dykes in the Sarnu-Dandali alkaline complex,Rajasthan, northwestern Deccan Traps

Whether swarms of preferentially oriented dykes are controlled by regional stress fields, or passively exploit basement structural fabric, is a much debated question, with support for either scenario in individual case studies. The Sarnu-Dandali alkaline complex, near the northwestern limit of the Deccan Traps continental flood basalt province, contains mafic to felsic alkaline volcano-plutonic rocks and carbonatites. The complex is situated near the northern end of the 600 km long, NNWe SSE-trending Barmer-Cambay rift. Mafic enclave swarms in the syenites suggest synplutonic mafic dykes injected into a largely liquid felsic magma chamber. Later coherent dykes in the complex, of all compositions and sizes,dominantly strike NNWe SSE, parallel to the Barmer-Cambay rift. The rift formed during two distinct episodes of extension, NWe SE in the early Cretaceous and NEe SW in the late Cretaceous. Control of the southern Indian Dharwar structural fabric on the rift trend, as speculated previously, is untenable,whereas the regional Precambrian basement trends（Aravalli and Malani） run NEe SW and NNEe SSW.We therefore suggest that the small-scale Sarnu-Dandali dykes and the much larger-scale BarmerCambay rift were not controlled by basement structure, but related to contemporaneous, late Cretaceous regional ENEe WSW extension, for which there is varied independent evidence.