High geostress,a typical attribute of tunnels located at significant depths,is crucial in causing stress-induced failure and influencing the stability of the tunnel crown.This study developed an analytical method for ...High geostress,a typical attribute of tunnels located at significant depths,is crucial in causing stress-induced failure and influencing the stability of the tunnel crown.This study developed an analytical method for the failure mechanism that occurs in deep-buried tunnel roofs,taking into account the influence of geostress.The limit analysis theory was utilized for deriving analytical solutions about the geometry of the collapsing surface and the limit supporting pressure.The collapsing surface obtained by the analytical solution was validated by the findings of the physical model test,which shows a high level of agreement with the actual one.An extensive investigation was done to explore the effects of the lateral pressure coefficients,the tunnel buried depth,the geological conditions of the surrounding rock,the long-short axis ratio,and the size of the tunnel profile.The findings indicate that an increase in the lateral pressure coefficient from 0.5 to 1.5 results in a reduction in the height of the collapsing zone by 2.08 m and the width of the collapsing zone by 1.15 m,while simultaneously increases the limit supporting pressure by 18.9%.The proposed upper bound method accurately determines the limit supporting pressure and the geometry of the collapsing surface,which aligns well with the results acquired through numerical modelling and on-site monitoring in actual engineering applications.The proposed analytical method can serve as a reference for similar crown failure issues of deep-buried tunnels.展开更多
Dissolution mechanism and favorable reservoir distribution prediction are the key problems restricting oil and gas exploration in deep-buried layers.In this paper,the Enping Formation and Zhuhai Formation in Baiyun Sa...Dissolution mechanism and favorable reservoir distribution prediction are the key problems restricting oil and gas exploration in deep-buried layers.In this paper,the Enping Formation and Zhuhai Formation in Baiyun Sag of South China Sea was taken as a target.Based on the thin section,scanning electron microscopy,X-ray diffraction,porosity/permeability measurement,and mercury injection,influencing factors of dissolution were examined,and a dissolution model was established.Further,high-quality reservoirs were predicted temporally and spatially.The results show that dissolved pores constituted the main space of the Paleogene sandstone reservoir.Dissolution primarily occurred in the coarse-and medium-grained sandstones in the subaerial and subaqueous distributary channels,while dissolution was limited in fine-grained sandstones and inequigranular sandstones.The main dissolved minerals were feldspar,tuffaceous matrix,and diagenetic cement.Kaolinization of feldspar and illitization of kaolinite are the main dissolution pathways,but they occur at various depths and temperatures with different geothermal gradients.Dissolution is controlled by four factors,in terms of depositional facies,source rock evolution,overpressure,and fault activities,which co-acted at the period of 23.8–13.8 Ma,and resulted into strong dissolution.Additionally,based on these factors,high-quality reservoirs of the Enping and Zhuhai formations are predicted in the northern slope,southwestern step zone,and Liuhua uplift in the Baiyun Sag.展开更多
Background Providing high-quality roughage is crucial for improvement of ruminant production because it is an essential component of their feed.Our previous study showed that feeding bio-fermented rice straw(BF)improv...Background Providing high-quality roughage is crucial for improvement of ruminant production because it is an essential component of their feed.Our previous study showed that feeding bio-fermented rice straw(BF)improved the feed intake and weight gain of sheep.However,it remains unclear why feeding BF to sheep increased their feed intake and weight gain.Therefore,the purposes of this research were to investigate how the rumen micro-biota and serum metabolome are dynamically changing after feeding BF,as well as how their changes influence the feed intake,digestibility,nutrient transport,meat quality and growth performances of sheep.Twelve growing Hu sheep were allocated into 3 groups:alfalfa hay fed group(AH:positive control),rice straw fed group(RS:negative control)and BF fed group(BF:treatment).Samples of rumen content,blood,rumen epithelium,muscle,feed offered and refusals were collected for the subsequent analysis.Results Feeding BF changed the microbial community and rumen fermentation,particularly increasing(P<0.05)relative abundance of Prevotella and propionate production,and decreasing(P<0.05)enteric methane yield.The histomorphology(height,width,area and thickness)of rumen papillae and gene expression for carbohydrate trans-port(MCT1),tight junction(claudin-1,claudin-4),and cell proliferation(CDK4,Cyclin A2,Cyclin E1)were improved(P<0.05)in sheep fed BF.Additionally,serum metabolome was also dynamically changed,which led to up-regulating(P<0.05)the primary bile acid biosynthesis and biosynthesis of unsaturated fatty acid in sheep fed BF.As a result,the higher(P<0.05)feed intake,digestibility,growth rate,feed efficiency,meat quality and mono-unsaturated fatty acid concentration in muscle,and the lower(P<0.05)feed cost per kg of live weight were achieved by feeding BF.Conclusions Feeding BF improved the growth performances and meat quality of sheep and reduced their feed cost.Therefore,bio-fermentation of rice straw could be an innovative way for improving ruminant production with mini-mizing production costs.展开更多
Soil salinity is a major limiting factor for crop production in coastal areas of Bangladesh. Cheap and sustainable management of soil salinity is hence most sought out topics in agricultural research. Conceptualizing ...Soil salinity is a major limiting factor for crop production in coastal areas of Bangladesh. Cheap and sustainable management of soil salinity is hence most sought out topics in agricultural research. Conceptualizing that idea in mind, a pot experiment was conducted in the Department of Soil, Water & Environment, University of Dhaka in order to analyze if common organic amendments (rice straw, saw dust) coupled with reduce photoperiod can mitigate salinity effect on the growth of bean (Phaseolus vulgaris). The experiment was set up following completely randomized design (CRD) with nine treatments and three replications containing Tc (Control), T1 (Ambient photoperiod + 110 mM Salinity treatment + Rice straw), T2 (Reduced photoperiod + 110 mM Salinity treatment + Rice straw), T3 (Ambient photoperiod + 220 mM Salinity treatment + Rice straw), T4 (Reduced photoperiod + 220 mM Salinity treatment + Rice straw), T5 (Ambient photoperiod + 110 mM Salinity treatment + Saw dust), T6 (Reduced photoperiod + 110 mM Salinity treatment + Saw dust), T7 (Ambient photoperiod + 220 mM Salinity treatment + Saw dust) and T8 (Reduced photoperiod + 220 mM Salinity treatment + Saw dust). Organic amendments were used separately at the rate of 12 ton/ha. The highest plant height (98.67 cm), root length (12.5 cm), pod number (10.33), leaf area (13.99 cm2), fresh weight (680 kg/ha), dry weight (316.67 kg/ha) were recorded with the treatment T1 while the second-best treatment was treatment T2 (with highest harvest index 0.040) and these results were statistically significant (p < 0.001). In post-harvest soil, pH, EC, OC, OM;available N, P, K, S;total Ca, Mg, Zn, Mn were increased significantly in treatment T1. The overall results illustrated that the best growth and yield performances were achieved in the treatment T1 and T2.展开更多
Biogasification of coal is important for clean utilization of coal. Experiments on the fermentation of single lignite, single straw and their mixture were performed to explore the variation characteristics of gas prod...Biogasification of coal is important for clean utilization of coal. Experiments on the fermentation of single lignite, single straw and their mixture were performed to explore the variation characteristics of gas production potential, microbial community and methanogenic metabolic pathways of mixture. Research has shown that mixed fermentation of lignite and straw significantly promoted biomethane production. The abundance of hydrolytic acidifying functional bacteria genera (Sphaerochaeta, Lentimicrobium) in mixed fermentation was higher than that in the fermentation of single lignite and single straw. The abundance of some key CAZy metabolic enzyme gene sequences in mixed fermentation group was increased, which was favorable to improve methane production. Aceticlastic methanogenesis was the most critical methanogenic pathway and acetic acid pathway was more competitive in methanogenic mode during peak fermentation. Macrogenomics provided theoretical support for the claim that mixed fermentation of coal and straw promoted biomethane metabolism, which was potentially valuable in expanding methanogenesis from mixed fermentation of lignite with different biomasses.展开更多
Straw return is a promising strategy for managing soil organic carbon(SOC)and improving yield stability.However,the optimal straw return strategy for sustainable crop production in the wheat(Triticum aestivum L.)-cott...Straw return is a promising strategy for managing soil organic carbon(SOC)and improving yield stability.However,the optimal straw return strategy for sustainable crop production in the wheat(Triticum aestivum L.)-cotton(Gossypium hirsutum L.)cropping system remains uncertain.The objective of this study was to quantify the long-term(10 years)impact of carbon(C)input on SOC sequestration,soil aggregation and crop yields in a wheat-cotton cropping system in the Yangtze River Valley,China.Five treatments were arranged with a single-factor randomized design as follows:no straw return(Control),return of wheat straw only(Wt),return of cotton straw only(Ct),return of 50%wheat and 50%cotton straw(Wh-Ch)and return of 100%wheat and 100%cotton straw(Wt-Ct).In comparison to the Control,the SOC content increased by 8.4 to 20.2%under straw return.A significant linear positive correlation between SOC sequestration and C input(1.42-7.19 Mg ha^(−1)yr^(−1))(P<0.05)was detected.The percentages of aggregates of sizes>2 and 1-2 mm at the 0-20 cm soil depth were also significantly elevated under straw return,with the greatest increase of the aggregate stability in the Wt-Ct treatment(28.1%).The average wheat yields increased by 12.4-36.0%and cotton yields increased by 29.4-73.7%,and significantly linear positive correlations were also detected between C input and the yields of wheat and cotton.The average sustainable yield index(SYI)reached a maximum value of 0.69 when the C input was 7.08 Mg ha^(−1)yr^(−1),which was close to the maximum value(SYI of 0.69,C input of 7.19 Mg ha^(−1)yr^(-1))in the Wt-Ct treatment.Overall,the return of both wheat and cotton straw was the best strategy for improving SOC sequestration,soil aggregation,yields and their sustainability in the wheat-cotton rotation system.展开更多
The combined effects of straw incorporation(SI)and polymer-coated urea(PCU)application on soil ammonia(NH_(3))and nitrous oxide(N_(2)O)emissions from agricultural fields have not been comprehensively evaluated in Nort...The combined effects of straw incorporation(SI)and polymer-coated urea(PCU)application on soil ammonia(NH_(3))and nitrous oxide(N_(2)O)emissions from agricultural fields have not been comprehensively evaluated in Northwest China.We conducted a two-year field experiment to assess the effects of combining SI with either uncoated urea(U)or PCU on soil NH_(3)emissions,N_(2)O emissions,winter wheat yields,yield-scaled NH_(3)(/NH_(3)),and yield-scaled N_(2)O(/N_(2)O).Five treatments were investigated,no nitrogen(N)fertilizer(N_(0)),U application at 150 kg N ha-1 with and without SI(SI+U and S_(0)+U),and PCU application at 150 kg N ha^(-1) with and without SI(SI+PCU and S_(0)+PCU).The results showed that the NH_(3);emissions increased by 20.98-34.35%following Sl compared to straw removal,mainly due to increases in soil ammonium(NH_(4)^(+)-N)content and water-flled pore space(WFPS).SI resulted in higher N_(2)O emissions than under the So scenario by 13.31-49.23%due to increases in soil inorganic N(SIN)contents,WFPS,and soil microbial biomass.In contrast,the PCU application reduced the SIN contents compared to the U application,reducing the NH_(3)and N_(2)O emissions by 45.99-58.07 and 18.08-53.04%,respectively.Moreover,no significant positive effects of the SI or PCU applications on the winter wheat yield were observed.The lowest /NH_(3) and /N_(2)O values were observed under the S_(0)+PCU and SI+PCU treatments.Our results suggest that single PCU applications and their combination with straw are the optimal agricultural strategies for mitigating gaseous N emissions and maintaining optimal winter wheat yields in Northwest China.展开更多
The mechanical properties are essentially different when rock material is subjected to loading or unloading conditions. In this study, loading and unloading tests with various confining pressures are conducted to inve...The mechanical properties are essentially different when rock material is subjected to loading or unloading conditions. In this study, loading and unloading tests with various confining pressures are conducted to investigate the mechanical properties of marble material samples taken from the deep diversion tunnels of Jinping II Hydropower Station. The stress-strain relationship, failure characteristics and strength criterion are compared and analyzed based on the experiment results. The results show: in the loading and unloading test, peak strength, lateral strain, axial strain and plastic deformation increase significantly as the confining pressure increases. Lateral strain increased significantly and obvious lateral dilatancy can be observed to the change of confining pressure; The fracture mode is mainly the single shear fracture for the triaxial compression test and post-peak test, angle between the failure surface and the ends of the rock material becomes smaller as the confining pressure increases. Hock-Brown strength criterion reflects the strength characteristics of marble material under two different unloading conditions, and has some supplementary effects to the rock material of mechanical field.展开更多
Fragaria vesca,commonly known as wild or woodland strawberry,is the most widely distributed diploid Fragaria species and is native to Europe and Asia.Because of its small plant size,low heterozygosity,and relative eas...Fragaria vesca,commonly known as wild or woodland strawberry,is the most widely distributed diploid Fragaria species and is native to Europe and Asia.Because of its small plant size,low heterozygosity,and relative ease of genetic transformation,F.vesca has been a model plant for fruit research since the publication of its Illumina-based genome in 2011.However,its genomic contribution to octoploid cultivated strawberry remains a long-standing question.Here,we de novo assembled and annotated a telomere-to-telomere,gap-free genome of F.vesca‘Hawaii 4’,with all seven chromosomes assembled into single contigs,providing the highest completeness and assembly quality to date.The gap-free genome is 220785082 bp in length and encodes 36173 protein-coding gene models,including 1153 newly annotated genes.All 14 telomeres and seven centromeres were annotated within the seven chromosomes.Among the three previously recognized wild diploid strawberry ancestors,F.vesca,F.iinumae,and F.viridis,phylogenomic analysis showed that F.vesca and F.viridis are the ancestors of the cultivated octoploid strawberry F.×ananassa,and F.vesca is its closest relative.Three subgenomes of F.×ananassa belong to the F.vesca group,and one is sister to F.viridis.We anticipate that this high-quality,telomere-to-telomere,gap-free F.vesca genome,combined with our phylogenomic inference of the origin of cultivated strawberry,will provide insight into the genomic evolution of Fragaria and facilitate strawberry genetics and molecular breeding.展开更多
Cultivated strawberry(Fragaria×ananassa),a perennial herb belonging to the family Rosaceae,is a complex octoploid with high heterozygosity at most loci.However,there is no research on the haplotype of the octoplo...Cultivated strawberry(Fragaria×ananassa),a perennial herb belonging to the family Rosaceae,is a complex octoploid with high heterozygosity at most loci.However,there is no research on the haplotype of the octoploid strawberry genome.Here we aimed to obtain a high-quality genome of the cultivated strawberry cultivar,“Yanli”,using single molecule real-time sequencing and high-throughput chromosome conformation capture technology.The“Yanli”genome was 823 Mb in size,with a long terminal repeat assembly index of 14.99.The genome was phased into two haplotypes,Hap1(825 Mb with contig N50 of 26.70 Mb)and Hap2(808 Mb with contig N50 of 27.51 Mb).Using the combination of Hap1 and Hap2,we obtained for the first time a haplotype-resolved genome with 56 chromosomes for the cultivated octoploid strawberry.We identified a∼10 Mb inversion and translocation on chromosome 2-1.104957 and 102356 protein-coding genes were annotated in Hap1 and Hap2,respectively.Analysis of the genes related to the anthocyanin biosynthesis pathway revealed the structural diversity and complexity in the expression of the alleles in the octoploid F.×ananassa genome.In summary,we obtained a high-quality haplotype-resolved genome assembly of F.×ananassa,which will provide the foundation for investigating gene function and evolution of the genome of cultivated octoploid strawberry.展开更多
Straw is a general term for the stem and leaf parts of mature crops,and is a multi-purpose renewable biomass energy resource in the agricultural ecosystem.The prospect of comprehensive utilization of straw has become ...Straw is a general term for the stem and leaf parts of mature crops,and is a multi-purpose renewable biomass energy resource in the agricultural ecosystem.The prospect of comprehensive utilization of straw has become broad with the development of agricultural production,the advancement of science and technology,and the improvement of the level of agricultural mechanization.The comprehensive utilization of straw plays an important role in enhancing the sustainable development ability of agricultural economy and improving the current situation of comprehensive utilization of agricultural resources in my country.This paper briefly combs the development history of straw and the prospect and current situation of comprehensive utilization,and expounds the separation technology of straw components,straw man-made panels,straw concrete,straw returning technology and oyster mushroom cultivation.It focuses on the description of the component separation technology of straw and the manufacturing process of straw-based panels.The different separation methods and separation effects of cellulose,hemicellulose and lignin were introduced in detail,and the static yield strength(MOR),internal bonding strength(IB)and water absorption thickness of several common straw-based panels were compared and studied(TS).Finally,it summarizes the benefit analysis of the comprehensive utilization of straw by scholars from the perspective of economics,and summarizes the corresponding measures based on their own views.展开更多
Straw returning to the field is a technical measure of crop production widely adopted in arid areas. It is unknown whether crop yield can be further increased by improving the eco-physiological characteristics when st...Straw returning to the field is a technical measure of crop production widely adopted in arid areas. It is unknown whether crop yield can be further increased by improving the eco-physiological characteristics when straw returning is applied in the crop production system. So, a three-year field experiment was conducted with various straw returning treatments for wheat production:(i) no-tillage with straw mulching(NTSM),(ii) no-tillage with straw standing(NTSS),(iii) conventional tillage with straw incorporation(CTS), and(iv) conventional tillage with no straw returning(CT, control). The eco-physiological and yield formation indicators were investigated to provide the basis for selecting the appropriate straw returning method to increase wheat yield and clarifying its regulation mechanism on eco-physiology. The results showed that NTSM and NTSS treatments had better regulation of eco-physiological characteristics and had a higher yield increase than CTS and CT. Meanwhile, NTSM had a relatively higher yield than NTSS through better regulation of eco-physiological characteristics. Compared to CT, the leaf area index of NTSM was decreased by 6.1–7.6% before the Feekes 10.0 stage of wheat, but that of NTSM was increased by 38.9–45.1% after the Feekes 10.0 stage. NTSM effectively regulated the dynamics of the photosynthetic source of green leaves during the wheat growth period. NTSM improved net photosynthetic rate by 10.2–21.4% and 11.0–21.6%, raised transpiration rate by 4.4–10.0% and 5.3–6.1%, increased leaf water use efficiency by 5.6–10.4% and 5.4–14.6%, at Feekes 11.0 and 11.2 stages of wheat, compared to CT, respectively. NTSM had higher leaf water potential(LWP) by 7.5–12.0% and soil water potential(SWP) by 8.9–24.0% from Feekes 10.3 to 11.2 stages of wheat than CT. Meanwhile, the absolute value of difference on LWP and SWP with NTSM was less than that with CT, indicating that NTSM was conducive to holding the stability of water demand for wheat plants and water supply of soil at arid conditions. Thus, NTSM had a greater grain yield of wheat by 18.6–27.3% than CT, and the high yield was attributed to the synchronous increase and cooperative development of ear number, grain number per ear, and 1 000-grain weight. NTSM had a positive effect on regulating the eco-physiological characteristics and can be recommended to enhance wheat grain yield in arid conditions.展开更多
Strawberry(Fragaria×ananassa)fruits are an excellent source of L-ascorbic acid(AsA),a powerful antioxidant for plants and humans.Identifying the genetic components underlying AsA accumulation is crucial for enhan...Strawberry(Fragaria×ananassa)fruits are an excellent source of L-ascorbic acid(AsA),a powerful antioxidant for plants and humans.Identifying the genetic components underlying AsA accumulation is crucial for enhancing strawberry nutritional quality.Here,we unravel the genetic architecture of AsA accumulation using an F1 population derived from parental lines‘Candonga’and‘Senga Sengana’,adapted to distinct Southern and Northern European areas.To account for environmental effects,the F1 and parental lines were grown and phenotyped in five locations across Europe(France,Germany,Italy,Poland and Spain).Fruit AsA content displayed normal distribution typical of quantitative traits and ranged five-fold,with significant differences among genotypes and environments.AsA content in each country and the average in all of them was used in combination with 6,974 markers for quantitative trait locus(QTL)analysis.Environmentally stable QTLs for AsA content were detected in linkage group(LG)3A,LG 5A,LG 5B,LG 6B and LG 7C.Candidate genes were identified within stable QTL intervals and expression analysis in lines with contrasting AsA content suggested that GDP-L-Galactose Phosphorylase FaGGP(3A),and the chloroplast-located AsA transporter gene FaPHT4;4(7C)might be the underlying genetic factors for QTLs on LG 3A and 7C,respectively.We show that recessive alleles of FaGGP(3A)inherited from both parental lines increase fruit AsA content.Furthermore,expression of FaGGP(3A)was two-fold higher in lines with high AsA.Markers here identified represent a useful resource for efficient selection of new strawberry cultivars with increased AsA content.展开更多
Inversion tillage with straw amendment is widely applied in northeastern China, and it can substantially increase the storage of carbon and improve multiple subsoil functions. Soil microorganisms are believed to be th...Inversion tillage with straw amendment is widely applied in northeastern China, and it can substantially increase the storage of carbon and improve multiple subsoil functions. Soil microorganisms are believed to be the key to this process,but research into their role in subsoil amelioration is limited. Therefore, a field experiment was conducted in 2018 in a region in northeastern China with Hapli-Udic Cambisol using four treatments: conventional tillage(CT, tillage to a depth of 15 cm with no straw incorporation), straw incorporation with conventional tillage(SCT, tillage to a depth of 15 cm),inversion tillage(IT, tillage to a depth of 35 cm) and straw incorporation with inversion tillage(SIT, tillage to a depth of 35 cm). The soils were managed by inversion to a depth of 15 or 35 cm every year after harvest. The results indicated that SIT improved soil multi-nutrient cycling variables and increased the availability of key nutrients such as soil organic carbon, total nitrogen, available nitrogen, available phosphorus and available potassium in both the topsoil and subsoil.In contrast to CT and SCT, SIT created a looser microbial network structure but with highly centralized clusters by reducing the topological properties of average connectivity and node number, and by increasing the average path length and the modularity. A Random Forest analysis found that the average path length and the clustering coefficient were the main determinants of soil multi-nutrient cycling. These findings suggested that SIT can be an effective option for improving soil multi-nutrient cycling and the structure of microbial networks, and they provide crucial information about the microbial strategies that drive the decomposition of straw in Hapli-Udic Cambisol.展开更多
Firmness is one of the most important fruit quality traits in strawberries.The postharvest shelf life of this soft fruit is highly limited by the loss of firmness,where cell wall disassembly plays an important role.Pr...Firmness is one of the most important fruit quality traits in strawberries.The postharvest shelf life of this soft fruit is highly limited by the loss of firmness,where cell wall disassembly plays an important role.Previous studies demonstrated that the polygalacturonase FaPG1 has a key role in remodelling pectins during strawberry softening.In this study,FaPG1 knockout strawberry plants have been generated using the CRISPR/Cas9 system delivered via Agrobacterium tumefaciens.Ten independent lines,cv.“Chandler”,were obtained,and all of them were successfully edited as determined by PCR amplification and T7 endonuclease assay.The targeted mutagenesis insertion and deletion rates were analyzed using targeted deep sequencing.The percentage of edited sequences varied from 47%up to almost 100%,being higher than 95%for seven of the selected lines.Phenotypic analyses showed that 7 out of the eight lines analyzed produced fruits significantly firmer than the control,ranging from 33 to 70%increase in firmness.There was a positive relationship between the degree of FaPG1 editing and the rise in fruit firmness.Minor changes were observed in other fruit quality traits,such as colour,soluble solids,titratable acidity or anthocyanin content.Edited fruits showed a reduced softening rate during postharvest,displayed a reduced transpirational water loss,and were less damaged by Botrytis cinerea inoculation.The analysis of four potential off-target sites revealed no mutation events.In conclusion,editing the FaPG1 gene using the CRISPR/Cas9 system is an efficient method for improving strawberry fruit firmness and shelf life.展开更多
Due to the existence of a large number of discontinuous fractures and interfaces in tunnel surrounding rocks,the groundwater inflow into tunnel generally presents significant anisotropy.Therefore,it is of great signif...Due to the existence of a large number of discontinuous fractures and interfaces in tunnel surrounding rocks,the groundwater inflow into tunnel generally presents significant anisotropy.Therefore,it is of great significance to consider the anisotropic permeability when dealing with water gushing-induced engineering accidents in water-rich mountain tunnels with large burial depth.In this study,based on the complex variable method and the seepage flow theory,a theoretical model of water inflow into a deep-buried circular tunnel in a fully saturated,anisotropic and semi-infinite aquifer is developed.The influence of grouted zone,initial support and secondary lining is fully considered.By comparison to the existing analytical methods and numerical results,the reliability of this proposed analytical solution is well validated.It is indicated from the parametric study that the groundwater inflow into tunnel presents an upward trend with an increasing value of the strata permeability in the vertical direction.Moreover,the water inflow rate and the total water head decrease with the growth of the thickness of grouting circle.It is suggested that reasonable grouting thickness and permeability should be controlled to enhance the grouting effect.This study provides a practical method for estimating the water inflow into a deep-buried,grouted and lined mountain tunnel considering the anisotropic strata permeability.展开更多
With the development circular economy, the use of agricultural waste to prepare biomass materials to remove pollutants has become a research hotspot. In this study, sunflower straw activated carbon (SSAC) was prepared...With the development circular economy, the use of agricultural waste to prepare biomass materials to remove pollutants has become a research hotspot. In this study, sunflower straw activated carbon (SSAC) was prepared by the one-step activation method, with sunflower straw (SS) used as the raw material and H3PO4 used as the activator. Four types of SSAC were prepared with impregnation ratios (weight of SS to weight of H3PO4) of 1:1, 1:2, 1:3, and 1:5, corresponding to SSAC1, SSAC2, SSAC3, and SSAC4, respectively. The adsorption process of acid fuchsin (AF) in water using the four types of SSAC was studied. The results showed that the impregnation ratio significantly affected the structure of the materials. The increase in the impregnation ratio increased the specific surface area and pore volume of SSAC and improved the adsorption capacity of AF. However, an impregnation ratio that was too large led to a decrease in specific surface area. SSAC3, with an impregnation ratio of 1:3, had the largest specific surface area (1 794.01 m2/g), and SSAC4, with an impregnation ratio of 1:5, exhibited the smallest microporosity (0.052 7 cm3/g) and the largest pore volume (2.549 cm3/g). The adsorption kinetics of AF using the four types of SSAC agreed with the quasi-second-order adsorption kinetic model. The Langmuir isotherm model was suitable to describe SSAC3 and SSAC4, and the Freundlich isotherm model was appropriate to describe SSAC1 and SSAC2. The result of thermodynamics showed that the adsorption process was spontaneous and endothermic. At 303 K, SSAC4 showed a removal rate of 97.73% for 200-mg/L AF with a maximum adsorption capacity of 2 763.36 mg/g, the highest among the four types of SSAC. This study showed that SAAC prepared by the H3PO4-based one-step activation method is a green and efficient carbon material and has significant application potential for the treatment of dye-containing wastewater.展开更多
This paper compares analytical and numerical methods by taking the forecasting of water yield of deep-buried iron mine in Yanzhou, Shandong as an example. Regarding the analytical method, the equation of infinite and ...This paper compares analytical and numerical methods by taking the forecasting of water yield of deep-buried iron mine in Yanzhou, Shandong as an example. Regarding the analytical method, the equation of infinite and bilateral water inflow boundary is used to forecast the water yield, and in the case of numerical simulation, we employed the GMS software to establish a model and further to forecast the water yield. On the one hand, through applying the analytical method, the maximum water yield of mine 1 500 m deep below the surface was calculated to be 13 645.17 m3/d; on the other hand, through adopting the numerical method, we obtained the predicted result of 3 816.16 m3/d. Meanwhile, by using the boundary generalization in the above-mentioned two methods, and through a comparative analysis of the actual hydro-geological conditions in this deep-buried mine, which also concerns the advantages and disadvantages of the two methods respectively, this paper draws the conclusion that the analytical method is only applicable in ideal conditions, but numerical method is eligible to be used in complex hydro-geological conditions. Therefore, it is more applicable to employ the numerical method to forecast water yield of deep-buried iron mine in Yanzhou, Shandong.展开更多
Zonal disintegration is the phenomenon of cyclical rupture zone and nonrupture zone in the surrounding rock of a deep-buried chamber,which is different from that of a shallow chamber.Based on the finite difference sof...Zonal disintegration is the phenomenon of cyclical rupture zone and nonrupture zone in the surrounding rock of a deep-buried chamber,which is different from that of a shallow chamber.Based on the finite difference software FLAC3D,the numerical simulation of surrounding rock with different mechanical parameters was conducted by using the SU model(Bilinear Strain-Softening Ubiquitous-Joint).The influences of buried depth,cohesion,and internal friction angle of surrounding rock on zonal disintegration were analyzed to reveal the influence law.The results show that:(1)after the chamber excavation,multiple rupture zones gradually extend from the chamber surface or adjacent periphery to the deep surrounding rock.In the extension process,a single rupture zone may be forked into two or even multiple rupture zones,which cross each other and form the zonal disintegration zone.(2)Zonal disintegration is affected by bothσ(in situ stress)and U_(cs)(uniaxial compression strength).Smaller U_(cs)and largerσwill lead to zonal disintegration.(3)The zoning fracture is not obvious in the case ofσ≤U_(cs).In the reverse case,zoning fracture appears remarkably in the surrounding rock around the chamber.These results reveal the influence law of zonal disintegration and provide theoretical support for the design of deep-buried chambers.展开更多
Complex weak structural planes and fault zones induce significant heterogeneity,discontinuity,and nonlinear characteristics of a rock mass.When an earthquake occurs,these characteristics lead to extremely complex seis...Complex weak structural planes and fault zones induce significant heterogeneity,discontinuity,and nonlinear characteristics of a rock mass.When an earthquake occurs,these characteristics lead to extremely complex seismic wave propagation and vibrational behaviors and thus pose a huge threat to the safety and stability of deep buried tunnels.To investigate the wave propagation in a rock mass with different structural planes and fault zones,this study first introduced the theory of elastic wave propagation and elastodynamic principles and used the Zoeppritz equation to describe wave field decomposition and develop a seismic wave response model accordingly.Then,a physical wave propagation model was constructed to investigate seismic waves passing through a fault,and dynamic damage was analyzed by using shaking table tests.Finally,stress wave attenuation and dynamic incompatible deformation mechanisms in a rock mass with fault zones were explored.The results indicate that under the action of weak structural planes,stress waves appear as a complex wave field decomposition phenomenon.When a stress wave spreads to a weak structural plane,its scattering may transform into a tensile wave,generating tensile stress and destabilizing the rock mass;wave dynamic energy is absorbed by a low-strength rock through wave scattering,which significantly weakens the seismic load.Wave propagation accelerates the initiation and expansion of internal defects in the rock mass and leads to a dynamic incompatible deformation.This is one of the main causes for large deformation and even instability within rock masses.These findings provide an important reference and guide with respect to stability analysis of rock mass with weak structural planes and fault zones.展开更多
基金supported partially by the National Natural Science Foundation of China(42277158,41972277,and U1934212)。
文摘High geostress,a typical attribute of tunnels located at significant depths,is crucial in causing stress-induced failure and influencing the stability of the tunnel crown.This study developed an analytical method for the failure mechanism that occurs in deep-buried tunnel roofs,taking into account the influence of geostress.The limit analysis theory was utilized for deriving analytical solutions about the geometry of the collapsing surface and the limit supporting pressure.The collapsing surface obtained by the analytical solution was validated by the findings of the physical model test,which shows a high level of agreement with the actual one.An extensive investigation was done to explore the effects of the lateral pressure coefficients,the tunnel buried depth,the geological conditions of the surrounding rock,the long-short axis ratio,and the size of the tunnel profile.The findings indicate that an increase in the lateral pressure coefficient from 0.5 to 1.5 results in a reduction in the height of the collapsing zone by 2.08 m and the width of the collapsing zone by 1.15 m,while simultaneously increases the limit supporting pressure by 18.9%.The proposed upper bound method accurately determines the limit supporting pressure and the geometry of the collapsing surface,which aligns well with the results acquired through numerical modelling and on-site monitoring in actual engineering applications.The proposed analytical method can serve as a reference for similar crown failure issues of deep-buried tunnels.
基金The National Natural Science Foundation of China under contract No.42202157the China National Offshore Oil Corporation Co.,Ltd.Major Production and Scientific Research Program under contract No.2019KT-SC-22。
文摘Dissolution mechanism and favorable reservoir distribution prediction are the key problems restricting oil and gas exploration in deep-buried layers.In this paper,the Enping Formation and Zhuhai Formation in Baiyun Sag of South China Sea was taken as a target.Based on the thin section,scanning electron microscopy,X-ray diffraction,porosity/permeability measurement,and mercury injection,influencing factors of dissolution were examined,and a dissolution model was established.Further,high-quality reservoirs were predicted temporally and spatially.The results show that dissolved pores constituted the main space of the Paleogene sandstone reservoir.Dissolution primarily occurred in the coarse-and medium-grained sandstones in the subaerial and subaqueous distributary channels,while dissolution was limited in fine-grained sandstones and inequigranular sandstones.The main dissolved minerals were feldspar,tuffaceous matrix,and diagenetic cement.Kaolinization of feldspar and illitization of kaolinite are the main dissolution pathways,but they occur at various depths and temperatures with different geothermal gradients.Dissolution is controlled by four factors,in terms of depositional facies,source rock evolution,overpressure,and fault activities,which co-acted at the period of 23.8–13.8 Ma,and resulted into strong dissolution.Additionally,based on these factors,high-quality reservoirs of the Enping and Zhuhai formations are predicted in the northern slope,southwestern step zone,and Liuhua uplift in the Baiyun Sag.
基金This research was supported by the National Natural Science Foundation of China(32061143034,32161143028)Tibet Regional Science and Technology Collaborative Innovation Project(QYXTZX-NQ2021-01)Fundamental Research Funds for the Central Universities(lzujbky-2022-ct04).
文摘Background Providing high-quality roughage is crucial for improvement of ruminant production because it is an essential component of their feed.Our previous study showed that feeding bio-fermented rice straw(BF)improved the feed intake and weight gain of sheep.However,it remains unclear why feeding BF to sheep increased their feed intake and weight gain.Therefore,the purposes of this research were to investigate how the rumen micro-biota and serum metabolome are dynamically changing after feeding BF,as well as how their changes influence the feed intake,digestibility,nutrient transport,meat quality and growth performances of sheep.Twelve growing Hu sheep were allocated into 3 groups:alfalfa hay fed group(AH:positive control),rice straw fed group(RS:negative control)and BF fed group(BF:treatment).Samples of rumen content,blood,rumen epithelium,muscle,feed offered and refusals were collected for the subsequent analysis.Results Feeding BF changed the microbial community and rumen fermentation,particularly increasing(P<0.05)relative abundance of Prevotella and propionate production,and decreasing(P<0.05)enteric methane yield.The histomorphology(height,width,area and thickness)of rumen papillae and gene expression for carbohydrate trans-port(MCT1),tight junction(claudin-1,claudin-4),and cell proliferation(CDK4,Cyclin A2,Cyclin E1)were improved(P<0.05)in sheep fed BF.Additionally,serum metabolome was also dynamically changed,which led to up-regulating(P<0.05)the primary bile acid biosynthesis and biosynthesis of unsaturated fatty acid in sheep fed BF.As a result,the higher(P<0.05)feed intake,digestibility,growth rate,feed efficiency,meat quality and mono-unsaturated fatty acid concentration in muscle,and the lower(P<0.05)feed cost per kg of live weight were achieved by feeding BF.Conclusions Feeding BF improved the growth performances and meat quality of sheep and reduced their feed cost.Therefore,bio-fermentation of rice straw could be an innovative way for improving ruminant production with mini-mizing production costs.
文摘Soil salinity is a major limiting factor for crop production in coastal areas of Bangladesh. Cheap and sustainable management of soil salinity is hence most sought out topics in agricultural research. Conceptualizing that idea in mind, a pot experiment was conducted in the Department of Soil, Water & Environment, University of Dhaka in order to analyze if common organic amendments (rice straw, saw dust) coupled with reduce photoperiod can mitigate salinity effect on the growth of bean (Phaseolus vulgaris). The experiment was set up following completely randomized design (CRD) with nine treatments and three replications containing Tc (Control), T1 (Ambient photoperiod + 110 mM Salinity treatment + Rice straw), T2 (Reduced photoperiod + 110 mM Salinity treatment + Rice straw), T3 (Ambient photoperiod + 220 mM Salinity treatment + Rice straw), T4 (Reduced photoperiod + 220 mM Salinity treatment + Rice straw), T5 (Ambient photoperiod + 110 mM Salinity treatment + Saw dust), T6 (Reduced photoperiod + 110 mM Salinity treatment + Saw dust), T7 (Ambient photoperiod + 220 mM Salinity treatment + Saw dust) and T8 (Reduced photoperiod + 220 mM Salinity treatment + Saw dust). Organic amendments were used separately at the rate of 12 ton/ha. The highest plant height (98.67 cm), root length (12.5 cm), pod number (10.33), leaf area (13.99 cm2), fresh weight (680 kg/ha), dry weight (316.67 kg/ha) were recorded with the treatment T1 while the second-best treatment was treatment T2 (with highest harvest index 0.040) and these results were statistically significant (p < 0.001). In post-harvest soil, pH, EC, OC, OM;available N, P, K, S;total Ca, Mg, Zn, Mn were increased significantly in treatment T1. The overall results illustrated that the best growth and yield performances were achieved in the treatment T1 and T2.
文摘Biogasification of coal is important for clean utilization of coal. Experiments on the fermentation of single lignite, single straw and their mixture were performed to explore the variation characteristics of gas production potential, microbial community and methanogenic metabolic pathways of mixture. Research has shown that mixed fermentation of lignite and straw significantly promoted biomethane production. The abundance of hydrolytic acidifying functional bacteria genera (Sphaerochaeta, Lentimicrobium) in mixed fermentation was higher than that in the fermentation of single lignite and single straw. The abundance of some key CAZy metabolic enzyme gene sequences in mixed fermentation group was increased, which was favorable to improve methane production. Aceticlastic methanogenesis was the most critical methanogenic pathway and acetic acid pathway was more competitive in methanogenic mode during peak fermentation. Macrogenomics provided theoretical support for the claim that mixed fermentation of coal and straw promoted biomethane metabolism, which was potentially valuable in expanding methanogenesis from mixed fermentation of lignite with different biomasses.
基金supported by the National Natural Science Foundation of China(32071968)the Jiangsu Agricultural Science and Technology Innovation Fund,China(CX(22)2015))the Jiangsu Collaborative Innovation Center for Modern Crop Production,China。
文摘Straw return is a promising strategy for managing soil organic carbon(SOC)and improving yield stability.However,the optimal straw return strategy for sustainable crop production in the wheat(Triticum aestivum L.)-cotton(Gossypium hirsutum L.)cropping system remains uncertain.The objective of this study was to quantify the long-term(10 years)impact of carbon(C)input on SOC sequestration,soil aggregation and crop yields in a wheat-cotton cropping system in the Yangtze River Valley,China.Five treatments were arranged with a single-factor randomized design as follows:no straw return(Control),return of wheat straw only(Wt),return of cotton straw only(Ct),return of 50%wheat and 50%cotton straw(Wh-Ch)and return of 100%wheat and 100%cotton straw(Wt-Ct).In comparison to the Control,the SOC content increased by 8.4 to 20.2%under straw return.A significant linear positive correlation between SOC sequestration and C input(1.42-7.19 Mg ha^(−1)yr^(−1))(P<0.05)was detected.The percentages of aggregates of sizes>2 and 1-2 mm at the 0-20 cm soil depth were also significantly elevated under straw return,with the greatest increase of the aggregate stability in the Wt-Ct treatment(28.1%).The average wheat yields increased by 12.4-36.0%and cotton yields increased by 29.4-73.7%,and significantly linear positive correlations were also detected between C input and the yields of wheat and cotton.The average sustainable yield index(SYI)reached a maximum value of 0.69 when the C input was 7.08 Mg ha^(−1)yr^(−1),which was close to the maximum value(SYI of 0.69,C input of 7.19 Mg ha^(−1)yr^(-1))in the Wt-Ct treatment.Overall,the return of both wheat and cotton straw was the best strategy for improving SOC sequestration,soil aggregation,yields and their sustainability in the wheat-cotton rotation system.
基金This work was supported by the National Key R&D Program of China(2021YFD1900700)the National Natural Science Foundation of China(52179046).
文摘The combined effects of straw incorporation(SI)and polymer-coated urea(PCU)application on soil ammonia(NH_(3))and nitrous oxide(N_(2)O)emissions from agricultural fields have not been comprehensively evaluated in Northwest China.We conducted a two-year field experiment to assess the effects of combining SI with either uncoated urea(U)or PCU on soil NH_(3)emissions,N_(2)O emissions,winter wheat yields,yield-scaled NH_(3)(/NH_(3)),and yield-scaled N_(2)O(/N_(2)O).Five treatments were investigated,no nitrogen(N)fertilizer(N_(0)),U application at 150 kg N ha-1 with and without SI(SI+U and S_(0)+U),and PCU application at 150 kg N ha^(-1) with and without SI(SI+PCU and S_(0)+PCU).The results showed that the NH_(3);emissions increased by 20.98-34.35%following Sl compared to straw removal,mainly due to increases in soil ammonium(NH_(4)^(+)-N)content and water-flled pore space(WFPS).SI resulted in higher N_(2)O emissions than under the So scenario by 13.31-49.23%due to increases in soil inorganic N(SIN)contents,WFPS,and soil microbial biomass.In contrast,the PCU application reduced the SIN contents compared to the U application,reducing the NH_(3)and N_(2)O emissions by 45.99-58.07 and 18.08-53.04%,respectively.Moreover,no significant positive effects of the SI or PCU applications on the winter wheat yield were observed.The lowest /NH_(3) and /N_(2)O values were observed under the S_(0)+PCU and SI+PCU treatments.Our results suggest that single PCU applications and their combination with straw are the optimal agricultural strategies for mitigating gaseous N emissions and maintaining optimal winter wheat yields in Northwest China.
基金Supported by National Natural Science Foundation of China(No.50974100)WHUT(NO.125106002)
文摘The mechanical properties are essentially different when rock material is subjected to loading or unloading conditions. In this study, loading and unloading tests with various confining pressures are conducted to investigate the mechanical properties of marble material samples taken from the deep diversion tunnels of Jinping II Hydropower Station. The stress-strain relationship, failure characteristics and strength criterion are compared and analyzed based on the experiment results. The results show: in the loading and unloading test, peak strength, lateral strain, axial strain and plastic deformation increase significantly as the confining pressure increases. Lateral strain increased significantly and obvious lateral dilatancy can be observed to the change of confining pressure; The fracture mode is mainly the single shear fracture for the triaxial compression test and post-peak test, angle between the failure surface and the ends of the rock material becomes smaller as the confining pressure increases. Hock-Brown strength criterion reflects the strength characteristics of marble material under two different unloading conditions, and has some supplementary effects to the rock material of mechanical field.
基金funding from the National Natural Science Foundation of China(32172614)a startup fund fromHainan University and a Hainan Province Science and Technology Special Fund(ZDYF2023XDNY050).
文摘Fragaria vesca,commonly known as wild or woodland strawberry,is the most widely distributed diploid Fragaria species and is native to Europe and Asia.Because of its small plant size,low heterozygosity,and relative ease of genetic transformation,F.vesca has been a model plant for fruit research since the publication of its Illumina-based genome in 2011.However,its genomic contribution to octoploid cultivated strawberry remains a long-standing question.Here,we de novo assembled and annotated a telomere-to-telomere,gap-free genome of F.vesca‘Hawaii 4’,with all seven chromosomes assembled into single contigs,providing the highest completeness and assembly quality to date.The gap-free genome is 220785082 bp in length and encodes 36173 protein-coding gene models,including 1153 newly annotated genes.All 14 telomeres and seven centromeres were annotated within the seven chromosomes.Among the three previously recognized wild diploid strawberry ancestors,F.vesca,F.iinumae,and F.viridis,phylogenomic analysis showed that F.vesca and F.viridis are the ancestors of the cultivated octoploid strawberry F.×ananassa,and F.vesca is its closest relative.Three subgenomes of F.×ananassa belong to the F.vesca group,and one is sister to F.viridis.We anticipate that this high-quality,telomere-to-telomere,gap-free F.vesca genome,combined with our phylogenomic inference of the origin of cultivated strawberry,will provide insight into the genomic evolution of Fragaria and facilitate strawberry genetics and molecular breeding.
基金This work was financially supported by National Natural Science Foundation of China(No.32130092,No.31872072)LiaoNing Revitalization Talents Pro-gram(No.XLYC1902069).
文摘Cultivated strawberry(Fragaria×ananassa),a perennial herb belonging to the family Rosaceae,is a complex octoploid with high heterozygosity at most loci.However,there is no research on the haplotype of the octoploid strawberry genome.Here we aimed to obtain a high-quality genome of the cultivated strawberry cultivar,“Yanli”,using single molecule real-time sequencing and high-throughput chromosome conformation capture technology.The“Yanli”genome was 823 Mb in size,with a long terminal repeat assembly index of 14.99.The genome was phased into two haplotypes,Hap1(825 Mb with contig N50 of 26.70 Mb)and Hap2(808 Mb with contig N50 of 27.51 Mb).Using the combination of Hap1 and Hap2,we obtained for the first time a haplotype-resolved genome with 56 chromosomes for the cultivated octoploid strawberry.We identified a∼10 Mb inversion and translocation on chromosome 2-1.104957 and 102356 protein-coding genes were annotated in Hap1 and Hap2,respectively.Analysis of the genes related to the anthocyanin biosynthesis pathway revealed the structural diversity and complexity in the expression of the alleles in the octoploid F.×ananassa genome.In summary,we obtained a high-quality haplotype-resolved genome assembly of F.×ananassa,which will provide the foundation for investigating gene function and evolution of the genome of cultivated octoploid strawberry.
基金supported by the National Natural Science Foundation of China(Nos.51878354&51308301)the Natural Science Foundation of Jiangsu Province(Nos.BK20181402&BK20130978)Qinglan Project of Jiangsu Higher Education Institutions.
文摘Straw is a general term for the stem and leaf parts of mature crops,and is a multi-purpose renewable biomass energy resource in the agricultural ecosystem.The prospect of comprehensive utilization of straw has become broad with the development of agricultural production,the advancement of science and technology,and the improvement of the level of agricultural mechanization.The comprehensive utilization of straw plays an important role in enhancing the sustainable development ability of agricultural economy and improving the current situation of comprehensive utilization of agricultural resources in my country.This paper briefly combs the development history of straw and the prospect and current situation of comprehensive utilization,and expounds the separation technology of straw components,straw man-made panels,straw concrete,straw returning technology and oyster mushroom cultivation.It focuses on the description of the component separation technology of straw and the manufacturing process of straw-based panels.The different separation methods and separation effects of cellulose,hemicellulose and lignin were introduced in detail,and the static yield strength(MOR),internal bonding strength(IB)and water absorption thickness of several common straw-based panels were compared and studied(TS).Finally,it summarizes the benefit analysis of the comprehensive utilization of straw by scholars from the perspective of economics,and summarizes the corresponding measures based on their own views.
基金National Natural Science Foundation of China (32101857, 32372238, and U21A20218)the Fuxi Young Talents Fund of Gansu Agricultural University, China (Gaufx-03Y10)+1 种基金the Science and Technology Program of Gansu Province, China (23JRRA1407)the ‘Double First-Class’ Key Scientific Research Project of Education Department in Gansu Province, China (GSSYLXM-02)。
文摘Straw returning to the field is a technical measure of crop production widely adopted in arid areas. It is unknown whether crop yield can be further increased by improving the eco-physiological characteristics when straw returning is applied in the crop production system. So, a three-year field experiment was conducted with various straw returning treatments for wheat production:(i) no-tillage with straw mulching(NTSM),(ii) no-tillage with straw standing(NTSS),(iii) conventional tillage with straw incorporation(CTS), and(iv) conventional tillage with no straw returning(CT, control). The eco-physiological and yield formation indicators were investigated to provide the basis for selecting the appropriate straw returning method to increase wheat yield and clarifying its regulation mechanism on eco-physiology. The results showed that NTSM and NTSS treatments had better regulation of eco-physiological characteristics and had a higher yield increase than CTS and CT. Meanwhile, NTSM had a relatively higher yield than NTSS through better regulation of eco-physiological characteristics. Compared to CT, the leaf area index of NTSM was decreased by 6.1–7.6% before the Feekes 10.0 stage of wheat, but that of NTSM was increased by 38.9–45.1% after the Feekes 10.0 stage. NTSM effectively regulated the dynamics of the photosynthetic source of green leaves during the wheat growth period. NTSM improved net photosynthetic rate by 10.2–21.4% and 11.0–21.6%, raised transpiration rate by 4.4–10.0% and 5.3–6.1%, increased leaf water use efficiency by 5.6–10.4% and 5.4–14.6%, at Feekes 11.0 and 11.2 stages of wheat, compared to CT, respectively. NTSM had higher leaf water potential(LWP) by 7.5–12.0% and soil water potential(SWP) by 8.9–24.0% from Feekes 10.3 to 11.2 stages of wheat than CT. Meanwhile, the absolute value of difference on LWP and SWP with NTSM was less than that with CT, indicating that NTSM was conducive to holding the stability of water demand for wheat plants and water supply of soil at arid conditions. Thus, NTSM had a greater grain yield of wheat by 18.6–27.3% than CT, and the high yield was attributed to the synchronous increase and cooperative development of ear number, grain number per ear, and 1 000-grain weight. NTSM had a positive effect on regulating the eco-physiological characteristics and can be recommended to enhance wheat grain yield in arid conditions.
基金supported by the European Union’s Horizon 2020 research and innovation program(GoodBerrygrant agreement number 679303)Agencia Estatal de Investigación(PID2019-111496RR-I00/AEI/10.13039/501100011033)and PR.AVA.AVA2019.034(IFAPA,FEDER funds)。
文摘Strawberry(Fragaria×ananassa)fruits are an excellent source of L-ascorbic acid(AsA),a powerful antioxidant for plants and humans.Identifying the genetic components underlying AsA accumulation is crucial for enhancing strawberry nutritional quality.Here,we unravel the genetic architecture of AsA accumulation using an F1 population derived from parental lines‘Candonga’and‘Senga Sengana’,adapted to distinct Southern and Northern European areas.To account for environmental effects,the F1 and parental lines were grown and phenotyped in five locations across Europe(France,Germany,Italy,Poland and Spain).Fruit AsA content displayed normal distribution typical of quantitative traits and ranged five-fold,with significant differences among genotypes and environments.AsA content in each country and the average in all of them was used in combination with 6,974 markers for quantitative trait locus(QTL)analysis.Environmentally stable QTLs for AsA content were detected in linkage group(LG)3A,LG 5A,LG 5B,LG 6B and LG 7C.Candidate genes were identified within stable QTL intervals and expression analysis in lines with contrasting AsA content suggested that GDP-L-Galactose Phosphorylase FaGGP(3A),and the chloroplast-located AsA transporter gene FaPHT4;4(7C)might be the underlying genetic factors for QTLs on LG 3A and 7C,respectively.We show that recessive alleles of FaGGP(3A)inherited from both parental lines increase fruit AsA content.Furthermore,expression of FaGGP(3A)was two-fold higher in lines with high AsA.Markers here identified represent a useful resource for efficient selection of new strawberry cultivars with increased AsA content.
基金funded by the National Key Research and Development Program of China (2022YFD1500100)the Strategic Priority Research Program of the Chinese Academy of Sciences (XDA28070100)+1 种基金the National Natural Science Foundation of China (41807085)the earmarked fund for China Agriculture Research System (CARS04)。
文摘Inversion tillage with straw amendment is widely applied in northeastern China, and it can substantially increase the storage of carbon and improve multiple subsoil functions. Soil microorganisms are believed to be the key to this process,but research into their role in subsoil amelioration is limited. Therefore, a field experiment was conducted in 2018 in a region in northeastern China with Hapli-Udic Cambisol using four treatments: conventional tillage(CT, tillage to a depth of 15 cm with no straw incorporation), straw incorporation with conventional tillage(SCT, tillage to a depth of 15 cm),inversion tillage(IT, tillage to a depth of 35 cm) and straw incorporation with inversion tillage(SIT, tillage to a depth of 35 cm). The soils were managed by inversion to a depth of 15 or 35 cm every year after harvest. The results indicated that SIT improved soil multi-nutrient cycling variables and increased the availability of key nutrients such as soil organic carbon, total nitrogen, available nitrogen, available phosphorus and available potassium in both the topsoil and subsoil.In contrast to CT and SCT, SIT created a looser microbial network structure but with highly centralized clusters by reducing the topological properties of average connectivity and node number, and by increasing the average path length and the modularity. A Random Forest analysis found that the average path length and the clustering coefficient were the main determinants of soil multi-nutrient cycling. These findings suggested that SIT can be an effective option for improving soil multi-nutrient cycling and the structure of microbial networks, and they provide crucial information about the microbial strategies that drive the decomposition of straw in Hapli-Udic Cambisol.
基金supported by the Ministerio de Ciencia,Innovación y Universidades and FEDER EU funds(grant numbers AGL2017-86531-C2-1R and PID2020-118468RB-C21)the University of Malaga(grant number B1-2020_09)+1 种基金CS-R was awarded a PhD Fellowship from the Ministerio de Ciencia,Innovación y Universidades(PRE2018-085509)PhD Program Advanced Biotechnology,University of Málaga.
文摘Firmness is one of the most important fruit quality traits in strawberries.The postharvest shelf life of this soft fruit is highly limited by the loss of firmness,where cell wall disassembly plays an important role.Previous studies demonstrated that the polygalacturonase FaPG1 has a key role in remodelling pectins during strawberry softening.In this study,FaPG1 knockout strawberry plants have been generated using the CRISPR/Cas9 system delivered via Agrobacterium tumefaciens.Ten independent lines,cv.“Chandler”,were obtained,and all of them were successfully edited as determined by PCR amplification and T7 endonuclease assay.The targeted mutagenesis insertion and deletion rates were analyzed using targeted deep sequencing.The percentage of edited sequences varied from 47%up to almost 100%,being higher than 95%for seven of the selected lines.Phenotypic analyses showed that 7 out of the eight lines analyzed produced fruits significantly firmer than the control,ranging from 33 to 70%increase in firmness.There was a positive relationship between the degree of FaPG1 editing and the rise in fruit firmness.Minor changes were observed in other fruit quality traits,such as colour,soluble solids,titratable acidity or anthocyanin content.Edited fruits showed a reduced softening rate during postharvest,displayed a reduced transpirational water loss,and were less damaged by Botrytis cinerea inoculation.The analysis of four potential off-target sites revealed no mutation events.In conclusion,editing the FaPG1 gene using the CRISPR/Cas9 system is an efficient method for improving strawberry fruit firmness and shelf life.
基金financially supported by the National Natural Science Foundation High Speed Railway Joint Fund of China(No.U1734205)the Open Research Fund Project of Key Laboratory of Geotechnical and Underground Engineering of Ministry of Education,Tongji University(No.KLETJGE-B2104)。
文摘Due to the existence of a large number of discontinuous fractures and interfaces in tunnel surrounding rocks,the groundwater inflow into tunnel generally presents significant anisotropy.Therefore,it is of great significance to consider the anisotropic permeability when dealing with water gushing-induced engineering accidents in water-rich mountain tunnels with large burial depth.In this study,based on the complex variable method and the seepage flow theory,a theoretical model of water inflow into a deep-buried circular tunnel in a fully saturated,anisotropic and semi-infinite aquifer is developed.The influence of grouted zone,initial support and secondary lining is fully considered.By comparison to the existing analytical methods and numerical results,the reliability of this proposed analytical solution is well validated.It is indicated from the parametric study that the groundwater inflow into tunnel presents an upward trend with an increasing value of the strata permeability in the vertical direction.Moreover,the water inflow rate and the total water head decrease with the growth of the thickness of grouting circle.It is suggested that reasonable grouting thickness and permeability should be controlled to enhance the grouting effect.This study provides a practical method for estimating the water inflow into a deep-buried,grouted and lined mountain tunnel considering the anisotropic strata permeability.
基金supported by the National Natural Science Foundation of China(Grant No.41865010)the 2020 Leading Talents of Young Science and Technology Talents in Colleges and Universities of the Inner Mongolia Autonomous Region(Grant No.NJYT-20-A04)the Project of the 10th Group of Grassland Talents of the Inner Mongolia Autonomous Region,the 2022 Inner Mongolia Outstanding Youth Fund Project,and the Key Research and Development and Achievement Transformation Program of the Inner Mongolia Autonomous Region in 2022(Grant No.2022YFHH0035).
文摘With the development circular economy, the use of agricultural waste to prepare biomass materials to remove pollutants has become a research hotspot. In this study, sunflower straw activated carbon (SSAC) was prepared by the one-step activation method, with sunflower straw (SS) used as the raw material and H3PO4 used as the activator. Four types of SSAC were prepared with impregnation ratios (weight of SS to weight of H3PO4) of 1:1, 1:2, 1:3, and 1:5, corresponding to SSAC1, SSAC2, SSAC3, and SSAC4, respectively. The adsorption process of acid fuchsin (AF) in water using the four types of SSAC was studied. The results showed that the impregnation ratio significantly affected the structure of the materials. The increase in the impregnation ratio increased the specific surface area and pore volume of SSAC and improved the adsorption capacity of AF. However, an impregnation ratio that was too large led to a decrease in specific surface area. SSAC3, with an impregnation ratio of 1:3, had the largest specific surface area (1 794.01 m2/g), and SSAC4, with an impregnation ratio of 1:5, exhibited the smallest microporosity (0.052 7 cm3/g) and the largest pore volume (2.549 cm3/g). The adsorption kinetics of AF using the four types of SSAC agreed with the quasi-second-order adsorption kinetic model. The Langmuir isotherm model was suitable to describe SSAC3 and SSAC4, and the Freundlich isotherm model was appropriate to describe SSAC1 and SSAC2. The result of thermodynamics showed that the adsorption process was spontaneous and endothermic. At 303 K, SSAC4 showed a removal rate of 97.73% for 200-mg/L AF with a maximum adsorption capacity of 2 763.36 mg/g, the highest among the four types of SSAC. This study showed that SAAC prepared by the H3PO4-based one-step activation method is a green and efficient carbon material and has significant application potential for the treatment of dye-containing wastewater.
文摘This paper compares analytical and numerical methods by taking the forecasting of water yield of deep-buried iron mine in Yanzhou, Shandong as an example. Regarding the analytical method, the equation of infinite and bilateral water inflow boundary is used to forecast the water yield, and in the case of numerical simulation, we employed the GMS software to establish a model and further to forecast the water yield. On the one hand, through applying the analytical method, the maximum water yield of mine 1 500 m deep below the surface was calculated to be 13 645.17 m3/d; on the other hand, through adopting the numerical method, we obtained the predicted result of 3 816.16 m3/d. Meanwhile, by using the boundary generalization in the above-mentioned two methods, and through a comparative analysis of the actual hydro-geological conditions in this deep-buried mine, which also concerns the advantages and disadvantages of the two methods respectively, this paper draws the conclusion that the analytical method is only applicable in ideal conditions, but numerical method is eligible to be used in complex hydro-geological conditions. Therefore, it is more applicable to employ the numerical method to forecast water yield of deep-buried iron mine in Yanzhou, Shandong.
基金National Natural Science Foundation of China,Grant/Award Number:52079068State Key Laboratory of Hydroscience and Hydraulic Engineering,Grant/Award Number:2021-KY-04Key Research and Development Plan of Ningxia Hui Autonomous Region,Grant/Award Number:2018BCG01003。
文摘Zonal disintegration is the phenomenon of cyclical rupture zone and nonrupture zone in the surrounding rock of a deep-buried chamber,which is different from that of a shallow chamber.Based on the finite difference software FLAC3D,the numerical simulation of surrounding rock with different mechanical parameters was conducted by using the SU model(Bilinear Strain-Softening Ubiquitous-Joint).The influences of buried depth,cohesion,and internal friction angle of surrounding rock on zonal disintegration were analyzed to reveal the influence law.The results show that:(1)after the chamber excavation,multiple rupture zones gradually extend from the chamber surface or adjacent periphery to the deep surrounding rock.In the extension process,a single rupture zone may be forked into two or even multiple rupture zones,which cross each other and form the zonal disintegration zone.(2)Zonal disintegration is affected by bothσ(in situ stress)and U_(cs)(uniaxial compression strength).Smaller U_(cs)and largerσwill lead to zonal disintegration.(3)The zoning fracture is not obvious in the case ofσ≤U_(cs).In the reverse case,zoning fracture appears remarkably in the surrounding rock around the chamber.These results reveal the influence law of zonal disintegration and provide theoretical support for the design of deep-buried chambers.
基金Fundamental Research Funds for the Central Universities,Grant/Award Number:B220202058National Natural Science Foundation of China,Grant/Award Number:41831278+1 种基金National Basic Research Program of China(973 Program),Grant/Award Number:2015CB057903ARC Future Fellowship,Grant/Award Number:FT140100019。
文摘Complex weak structural planes and fault zones induce significant heterogeneity,discontinuity,and nonlinear characteristics of a rock mass.When an earthquake occurs,these characteristics lead to extremely complex seismic wave propagation and vibrational behaviors and thus pose a huge threat to the safety and stability of deep buried tunnels.To investigate the wave propagation in a rock mass with different structural planes and fault zones,this study first introduced the theory of elastic wave propagation and elastodynamic principles and used the Zoeppritz equation to describe wave field decomposition and develop a seismic wave response model accordingly.Then,a physical wave propagation model was constructed to investigate seismic waves passing through a fault,and dynamic damage was analyzed by using shaking table tests.Finally,stress wave attenuation and dynamic incompatible deformation mechanisms in a rock mass with fault zones were explored.The results indicate that under the action of weak structural planes,stress waves appear as a complex wave field decomposition phenomenon.When a stress wave spreads to a weak structural plane,its scattering may transform into a tensile wave,generating tensile stress and destabilizing the rock mass;wave dynamic energy is absorbed by a low-strength rock through wave scattering,which significantly weakens the seismic load.Wave propagation accelerates the initiation and expansion of internal defects in the rock mass and leads to a dynamic incompatible deformation.This is one of the main causes for large deformation and even instability within rock masses.These findings provide an important reference and guide with respect to stability analysis of rock mass with weak structural planes and fault zones.