Post-acute ischemic stroke hyperglycemia increases the risk of hemorrhagic transformation,which is associated with blood-brain barrier disruption.Brain microvascular endothelial cells are a major component of the bloo...Post-acute ischemic stroke hyperglycemia increases the risk of hemorrhagic transformation,which is associated with blood-brain barrier disruption.Brain microvascular endothelial cells are a major component of the blood-brain barrier.Intercellular mitochondrial transfer has emerged as a novel paradigm for repairing cells with mitochondrial dysfunction.In this study,we first investigated whether mitochondrial transfer exists between brain microvascular endothelial cells,and then investigated the effects of post-acute ischemic stroke hyperglycemia on mitochondrial transfer between brain microvascular endothelial cells.We found that healthy brain microvascular endothelial cells can transfer intact mitochondria to oxygen glucose deprivation-injured brain microvascular endothelial cells.However,post-oxygen glucose deprivation hyperglycemia hindered mitochondrial transfer and exacerbated mitochondrial dysfunction.We established an in vitro brain microvascular endothelial cell model of the blood-brain barrier.We found that post-acute ischemic stroke hyperglycemia reduced the overall energy metabolism levels of brain microvascular endothelial cells and increased permeability of the blood-brain barrier.In a clinical study,we retrospectively analyzed the relationship between post-acute ischemic stroke hyperglycemia and the severity of hemorrhagic transformation.We found that post-acute ischemic stroke hyperglycemia serves as an independent predictor of severe hemorrhagic transformation.These findings suggest that post-acute ischemic stroke hyperglycemia can aggravate disruption of the blood-brain barrier by inhibiting mitochondrial transfer.展开更多
Background Several studies have reported an association of hyperglycemia with increased mortality and complications in hospital patients with acute coronary syndrome （ACS）. However, the influence of stress hyperglyc...Background Several studies have reported an association of hyperglycemia with increased mortality and complications in hospital patients with acute coronary syndrome （ACS）. However, the influence of stress hyperglycemia （SH） on the medium- and long-term prognoses in ACS patients has not yet been determined. Methods Random venous blood glucose levels were determined in 433 ACS patients and the patients were divided into two groups based on blood glucose results and disease histories. The 171 patients included in the experimental group had no history of diabetes, had no diabetes and/or glucose metabolism disorders during hospitalization and follow-up and had fasting blood glucose levels of I〉 7.0 mmol/L and random blood glucose levels 1〉 11.1 mmol/L. The 262 patients included in the control group had no history of diabetes, had no diabetes and/or glucose metabolism disorders during hospitalization and follow-up, and had fasting blood glucose levels 〈 6.1 mmoL/L and random blood glucose levels 〈 7.8 mmol/L. Basic clinical information, coronary angiographic lesion characteristics, PCI success rate, complication rate, incidence and morbidity rate of cardiovascular events during the hospitalization period and 6 years of follow-up were compared between the two groups. Results There was no significant difference in age, gender or disease history between the two groups. The triglyceride levels and the left ventricular ejection fractions were significantly higher （P = 0.00） and significantly lower （P = 0.03） in the experimental group than in the control group, respectively. Both groups were subjected to coronary angiography and PCI. The PCI success rates of the two groups did not differ significantly （P = 0.63）. The experimental group had more type B2 lesions, but fewer type A lesions compared with the control group. The experimental group had significantly more stents implanted compared with the control group （P 〈 0.05）. The cardiovascular events were significantly increased （P = 0.01） in the experimental group compared with the control group 1 year after discharge. The incidence of cardiovascular events did not differ significantly between the two groups 1-2 years after discharge. The event incidences were significantly higher （P -- 0.05） in the experimental group than in the control group at the end of follow-up. The experimental group was more prone to myocardial infarction and ischemic target vessel revascularization than the control group, but the control group was more prone to unstable stenocardia and heart failure than the experimental group. The psychogenic mortality of the two groups were similar. The interval and the overall mortality rates （P = 0.054） of the two groups were also similar. Conclusions Patients with ACS complicated by SH were more likely to have cardiovascular events 1 year after PCI, whereas SH did not affect the mid- or long-term prognoses of these patients. SH is an independent risk factor for cardiovascular events.展开更多
The aim of this study was to identify the correlation between stress, lifestyle, and hyperglycemia among middle-aged Japanese male workers. We also analyzed the obese (OB) and non-obese (non- OB) groups pertaining to ...The aim of this study was to identify the correlation between stress, lifestyle, and hyperglycemia among middle-aged Japanese male workers. We also analyzed the obese (OB) and non-obese (non- OB) groups pertaining to the risk of hyperglycemia. A total of 353 male employees aged between 50 and 59 years taking health checkup sat a company in Japan were examined. The data were collected using validated scales of occupational stress and medical examination. Of the 353 employees, 335 (effective response rate 95%) were analyzed. “Support from colleagues” and “reward from work” reported by the OB group were lower than the non-OB group. The items “eating until satiety” and “having greasy meal often” were significantly more common in the OB group than in the non-OB group. There was a significant correlation between less sleep time and hyperglycemia in the OB group than in the non-OB group. The non-OB group reported more overtime hours than the OB group. Hyperglycemia in the non-OB group was positively correlated with long working hours, “workload,” and “mental workload.” The results indicated that the OB group would benefit from lifestyle interventions, for example, improvement in sleep time and eating habits may prevent hyperglycemia and eventually in obesity. Furthermore, it was suggested that stress in response to “workload” and “mental workload” owing to long working hours leads to hyperglycemia in the non-OB group. Therefore, the improvement of the workplace environment, reducing the number of hours at work, and stress management are required to prevent hyperglycemia in the non-OB group.展开更多
目的 探讨血清可溶性晚期糖基化终末产物受体(soluble receptor for advanced glycation end products,sRAGE)联合内皮细胞特异性分子-1(endothelial cell specific molecules-1,ESM-1)对重症急性胰腺炎(severe acute pancreatitis,SAP...目的 探讨血清可溶性晚期糖基化终末产物受体(soluble receptor for advanced glycation end products,sRAGE)联合内皮细胞特异性分子-1(endothelial cell specific molecules-1,ESM-1)对重症急性胰腺炎(severe acute pancreatitis,SAP)合并应激性高血糖患者预后的预测价值。方法 选取2021年7月至2023年1月临汾市中心医院重症医学科的SAP合并应激性高血糖患者105例,根据患者的预后(对症治疗后28 d生存情况)分为死亡组(39例)和存活组(66例)。分析SAP合并应激性高血糖患者预后的影响因素及血清sRAGE联合ESM-1对患者预后的预测价值。结果 105例患者中男61例、女44例;年龄22~69岁,平均(47.6±8.9)岁。多因素logistic回归分析结果显示,多个器官功能障碍(OR=4.845,95%CI:2.166~8.130,P=0.030)、急性生理和慢性健康评分Ⅱ得分越高(OR=1.872,95%CI:1.207~2.902,P=0.005)、24 h随机空腹血糖越高(OR=1.381,95%CI:1.094~1.743,P=0.007)、sRAGE水平越高(OR=1.017,95%CI:1.007~1.027,P=0.001)、ESM-1水平越高(OR=1.074,95%CI:1.027~1.123,P=0.002)的SAP合并应激性高血糖患者更容易死亡。ROC曲线分析结果显示,血清sRAGE联合ESM-1检测预测SAP合并应激性高血糖患者死亡的AUC为0.882(95%CI:0.804~0.936,P<0.001),血清sRAGE和ESM-1单独预测的AUC分别为0.784(95%CI:0.693~0.859,P<0.001)和0.780(95%CI:0.689~0.855,P<0.001)。结论 SAP合并应激性高血糖患者的血清sRAGE、ESM-1浓度升高与不良预后有关,血清sRAGE联合ESM-1检测对SAP合并应激性高血糖患者预后的预测价值较高。展开更多
基金supported by the Ningbo Public Welfare Science and Technology Program,No.2022S023(to JY)Ningbo Natural Science Foundation,No.2022J211(to JS)+2 种基金Ningbo Medical and Health Brand Discipline,No.PPXK2018-04(to XG)Ningbo Top Medical and Health Research Program,No.2022020304(to XG)Key Laboratory of Precision Medicine for Atherosclerotic Diseases of Zhejiang Province,No.2022E10026(to YH)。
文摘Post-acute ischemic stroke hyperglycemia increases the risk of hemorrhagic transformation,which is associated with blood-brain barrier disruption.Brain microvascular endothelial cells are a major component of the blood-brain barrier.Intercellular mitochondrial transfer has emerged as a novel paradigm for repairing cells with mitochondrial dysfunction.In this study,we first investigated whether mitochondrial transfer exists between brain microvascular endothelial cells,and then investigated the effects of post-acute ischemic stroke hyperglycemia on mitochondrial transfer between brain microvascular endothelial cells.We found that healthy brain microvascular endothelial cells can transfer intact mitochondria to oxygen glucose deprivation-injured brain microvascular endothelial cells.However,post-oxygen glucose deprivation hyperglycemia hindered mitochondrial transfer and exacerbated mitochondrial dysfunction.We established an in vitro brain microvascular endothelial cell model of the blood-brain barrier.We found that post-acute ischemic stroke hyperglycemia reduced the overall energy metabolism levels of brain microvascular endothelial cells and increased permeability of the blood-brain barrier.In a clinical study,we retrospectively analyzed the relationship between post-acute ischemic stroke hyperglycemia and the severity of hemorrhagic transformation.We found that post-acute ischemic stroke hyperglycemia serves as an independent predictor of severe hemorrhagic transformation.These findings suggest that post-acute ischemic stroke hyperglycemia can aggravate disruption of the blood-brain barrier by inhibiting mitochondrial transfer.
基金supported by Medical Scientific Research Foundation of Guangdong Province,China(No.B2013401)
文摘Background Several studies have reported an association of hyperglycemia with increased mortality and complications in hospital patients with acute coronary syndrome （ACS）. However, the influence of stress hyperglycemia （SH） on the medium- and long-term prognoses in ACS patients has not yet been determined. Methods Random venous blood glucose levels were determined in 433 ACS patients and the patients were divided into two groups based on blood glucose results and disease histories. The 171 patients included in the experimental group had no history of diabetes, had no diabetes and/or glucose metabolism disorders during hospitalization and follow-up and had fasting blood glucose levels of I〉 7.0 mmol/L and random blood glucose levels 1〉 11.1 mmol/L. The 262 patients included in the control group had no history of diabetes, had no diabetes and/or glucose metabolism disorders during hospitalization and follow-up, and had fasting blood glucose levels 〈 6.1 mmoL/L and random blood glucose levels 〈 7.8 mmol/L. Basic clinical information, coronary angiographic lesion characteristics, PCI success rate, complication rate, incidence and morbidity rate of cardiovascular events during the hospitalization period and 6 years of follow-up were compared between the two groups. Results There was no significant difference in age, gender or disease history between the two groups. The triglyceride levels and the left ventricular ejection fractions were significantly higher （P = 0.00） and significantly lower （P = 0.03） in the experimental group than in the control group, respectively. Both groups were subjected to coronary angiography and PCI. The PCI success rates of the two groups did not differ significantly （P = 0.63）. The experimental group had more type B2 lesions, but fewer type A lesions compared with the control group. The experimental group had significantly more stents implanted compared with the control group （P 〈 0.05）. The cardiovascular events were significantly increased （P = 0.01） in the experimental group compared with the control group 1 year after discharge. The incidence of cardiovascular events did not differ significantly between the two groups 1-2 years after discharge. The event incidences were significantly higher （P -- 0.05） in the experimental group than in the control group at the end of follow-up. The experimental group was more prone to myocardial infarction and ischemic target vessel revascularization than the control group, but the control group was more prone to unstable stenocardia and heart failure than the experimental group. The psychogenic mortality of the two groups were similar. The interval and the overall mortality rates （P = 0.054） of the two groups were also similar. Conclusions Patients with ACS complicated by SH were more likely to have cardiovascular events 1 year after PCI, whereas SH did not affect the mid- or long-term prognoses of these patients. SH is an independent risk factor for cardiovascular events.
文摘The aim of this study was to identify the correlation between stress, lifestyle, and hyperglycemia among middle-aged Japanese male workers. We also analyzed the obese (OB) and non-obese (non- OB) groups pertaining to the risk of hyperglycemia. A total of 353 male employees aged between 50 and 59 years taking health checkup sat a company in Japan were examined. The data were collected using validated scales of occupational stress and medical examination. Of the 353 employees, 335 (effective response rate 95%) were analyzed. “Support from colleagues” and “reward from work” reported by the OB group were lower than the non-OB group. The items “eating until satiety” and “having greasy meal often” were significantly more common in the OB group than in the non-OB group. There was a significant correlation between less sleep time and hyperglycemia in the OB group than in the non-OB group. The non-OB group reported more overtime hours than the OB group. Hyperglycemia in the non-OB group was positively correlated with long working hours, “workload,” and “mental workload.” The results indicated that the OB group would benefit from lifestyle interventions, for example, improvement in sleep time and eating habits may prevent hyperglycemia and eventually in obesity. Furthermore, it was suggested that stress in response to “workload” and “mental workload” owing to long working hours leads to hyperglycemia in the non-OB group. Therefore, the improvement of the workplace environment, reducing the number of hours at work, and stress management are required to prevent hyperglycemia in the non-OB group.
