[ Objective] To develop a rapid, sensitive, specific and accurate diagnostic method for subclinical mastitis in dairy cow. [ Method] A total of 70 samples of suspected clinical mastitis cows were detected in gene leve...[ Objective] To develop a rapid, sensitive, specific and accurate diagnostic method for subclinical mastitis in dairy cow. [ Method] A total of 70 samples of suspected clinical mastitis cows were detected in gene level by multiple PCR. [Result] The 58 samples were positive with the developed multiple PCR method and the positive rate was 82.5% （58/70）. The 53 samples were positive with the traditional biochemical method and the positive rate was 75.5% （53/70）. As a result, the coincidence rate of both methods was 92%. [ Conclusion] The multiple PCR method is rapid and specific for the detection of pathogenic subclinical mastitis in dairy cow.展开更多
In this study, the milk samples of 1 021 cows in eight dairy farms in Eastern Hebei Province were collected and detected with LMT reagent and somatic cell count for subclinical mastitis. Pathogenic bacteria in subclin...In this study, the milk samples of 1 021 cows in eight dairy farms in Eastern Hebei Province were collected and detected with LMT reagent and somatic cell count for subclinical mastitis. Pathogenic bacteria in subclinical mastitis positive milk samples were isolated and identified.The results showed that 60.63%（619/1 021） of the sampled cows were diagnosed with subclinical mastitis, and mixed infections accounted for 88.21%（546/619） of the cases. In addition, 82 strains of 14 species were isolated from the subclinical mastitis positive milk samples, including 36 strains of Staphylococcus（43.90%）, 33 strains of Streptococcus（40.24%）, 8 strains of Enterobacteriaceae（9.76%） and 5 strains of Corynebacterium（6.10%）, respectively. The results proved that Staphylococcus aureus and Streptococcus agalactiae are the main pathogenic bacteria causing bovine subclinical mastitis in Eastern Hebei Province.展开更多
基金supported by Scientific and Technological De-velopment Project of Shijiazhuang (20091126A)
文摘[ Objective] To develop a rapid, sensitive, specific and accurate diagnostic method for subclinical mastitis in dairy cow. [ Method] A total of 70 samples of suspected clinical mastitis cows were detected in gene level by multiple PCR. [Result] The 58 samples were positive with the developed multiple PCR method and the positive rate was 82.5% （58/70）. The 53 samples were positive with the traditional biochemical method and the positive rate was 75.5% （53/70）. As a result, the coincidence rate of both methods was 92%. [ Conclusion] The multiple PCR method is rapid and specific for the detection of pathogenic subclinical mastitis in dairy cow.
基金Supported by Beef Cattle Disease Prevention and Control Project of Agricultural Industry Technology System of Hebei ProvinceThe Fund of Qinhuangdao Science and Technology Bureau(200901A070)China Spark Program(2012GA620002)
文摘In this study, the milk samples of 1 021 cows in eight dairy farms in Eastern Hebei Province were collected and detected with LMT reagent and somatic cell count for subclinical mastitis. Pathogenic bacteria in subclinical mastitis positive milk samples were isolated and identified.The results showed that 60.63%（619/1 021） of the sampled cows were diagnosed with subclinical mastitis, and mixed infections accounted for 88.21%（546/619） of the cases. In addition, 82 strains of 14 species were isolated from the subclinical mastitis positive milk samples, including 36 strains of Staphylococcus（43.90%）, 33 strains of Streptococcus（40.24%）, 8 strains of Enterobacteriaceae（9.76%） and 5 strains of Corynebacterium（6.10%）, respectively. The results proved that Staphylococcus aureus and Streptococcus agalactiae are the main pathogenic bacteria causing bovine subclinical mastitis in Eastern Hebei Province.
