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Multiple PCR for Detection of Subclinical Mastitis in Dairy Cow
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作者 XIAO Ying GU Wei-na +2 位作者 QIAN Ming-ming LIU Lei ZHAO Bao-hua 《Animal Husbandry and Feed Science》 CAS 2010年第4期11-13,17,共4页
[ Objective] To develop a rapid, sensitive, specific and accurate diagnostic method for subclinical mastitis in dairy cow. [ Method] A total of 70 samples of suspected clinical mastitis cows were detected in gene leve... [ Objective] To develop a rapid, sensitive, specific and accurate diagnostic method for subclinical mastitis in dairy cow. [ Method] A total of 70 samples of suspected clinical mastitis cows were detected in gene level by multiple PCR. [Result] The 58 samples were positive with the developed multiple PCR method and the positive rate was 82.5% (58/70). The 53 samples were positive with the traditional biochemical method and the positive rate was 75.5% (53/70). As a result, the coincidence rate of both methods was 92%. [ Conclusion] The multiple PCR method is rapid and specific for the detection of pathogenic subclinical mastitis in dairy cow. 展开更多
关键词 Dairy cow subclinical mastitis Multiple PCR DETECTION
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Isolation and Identification of Pathogenic Bacteria Causing Bovine Subclinical Mastitis in Eastern Hebei Province
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作者 Gao Guisheng Wu Tonglei +7 位作者 Zhang Zhiqiang Yang Zongze Fu Zhixin Zou Fengping Gao Guangping An Jiwei Yao Weiping Shi Qiumei 《Animal Husbandry and Feed Science》 CAS 2018年第1期65-67,共3页
In this study, the milk samples of 1 021 cows in eight dairy farms in Eastern Hebei Province were collected and detected with LMT reagent and somatic cell count for subclinical mastitis. Pathogenic bacteria in subclin... In this study, the milk samples of 1 021 cows in eight dairy farms in Eastern Hebei Province were collected and detected with LMT reagent and somatic cell count for subclinical mastitis. Pathogenic bacteria in subclinical mastitis positive milk samples were isolated and identified.The results showed that 60.63%(619/1 021) of the sampled cows were diagnosed with subclinical mastitis, and mixed infections accounted for 88.21%(546/619) of the cases. In addition, 82 strains of 14 species were isolated from the subclinical mastitis positive milk samples, including 36 strains of Staphylococcus(43.90%), 33 strains of Streptococcus(40.24%), 8 strains of Enterobacteriaceae(9.76%) and 5 strains of Corynebacterium(6.10%), respectively. The results proved that Staphylococcus aureus and Streptococcus agalactiae are the main pathogenic bacteria causing bovine subclinical mastitis in Eastern Hebei Province. 展开更多
关键词 Dairy cow subclinical mastitis Pathogen Isolation and identification
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