Machine learning-enhanced Monte Carlo and subset simulations for advanced risk assessment in transportation infrastructure

The maintenance of safety and dependability in rail and road embankments is of utmost importance in order to facilitate the smooth operation of transportation networks.This study introduces a comprehensive methodology for soil slope stability evaluation,employing Monte Carlo Simulation(MCS)and Subset Simulation(SS)with the"UPSS 3.0 Add-in"in MS-Excel.Focused on an 11.693-meter embankment with a soil slope(inclination ratio of 2H:1V),the investigation considers earthquake coefficients(kh)and pore water pressure ratios(ru)following Indian zoning requirements.The chance of slope failure showed a considerable increase as the Coefficient of Variation(COV),seismic coefficients(kh),and pore water pressure ratios(ru)experienced an escalation.The SS approach showed exceptional efficacy in calculating odds of failure that are notably low.Within computational modeling,the study optimized the worst-case scenario using ANFIS-GA,ANFIS-GWO,ANFIS-PSO,and ANFIS-BBO models.The ANFIS-PSO model exhibits exceptional accuracy(training R2=0.9011,RMSE=0.0549;testing R2=0.8968,RMSE=0.0615),emerging as the most promising.This study highlights the significance of conducting thorough risk assessments and offers practical insights into evaluating and improving the stability of soil slopes in transportation infrastructure.These findings contribute to the enhancement of safety and reliability in real-world situations.

Correlation of tumor-associated macrophage density and proportion of M2 subtypes with the pathological stage of colorectal cancer

BACKGROUND Colorectal cancer(CRC)is a prevalent global malignancy with complex prognostic factors.Tumor-associated macrophages(TAMs)have shown paradoxical associations with CRC survival,particularly concerning the M2 subset.AIM We aimed to establish a simplified protocol for quantifying M2-like TAMs and explore their correlation with clinicopathological factors.METHODS A cross-sectional study included histopathological assessment of paraffinembedded tissue blocks obtained from 43 CRC patients.Using CD68 and CD163 immunohistochemistry,we quantified TAMs in tumor stroma and front,focusing on M2 proportion.Demographic,histopathological,and clinical parameters were collected.RESULTS TAM density was significantly higher at the tumor front,with the M2 proportion three times greater in both zones.The tumor front had a higher M2 proportion,which correlated significantly with advanced tumor stage(P=0.04),pathological nodal involvement(P=0.04),and lymphovascular invasion(LVI,P=0.01).However,no significant association was found between the M2 proportion in the tumor stroma and clinicopathological factors.CONCLUSION Our study introduces a simplified protocol for quantifying M2-like TAMs in CRC tissue samples.We demonstrated a significant correlation between an increased M2 proportion at the tumor front and advanced tumor stage,nodal involvement,and LVI.This suggests that M2-like TAMs might serve as potential indicators of disease progression in CRC,warranting further investigation and potential clinical application.

Comparison of Block Design Nonparametric Subset Selection Rules Based on Alternative Scoring Rules

This article compares the size of selected subsets using nonparametric subset selection rules with two different scoring rules for the observations. The scoring rules are based on the expected values of order statistics of the uniform distribution (yielding rank values) and of the normal distribution (yielding normal score values). The comparison is made using state motor vehicle traffic fatality rates, published in a 2016 article, with fifty-one states (including DC as a state) and over a nineteen-year period (1994 through 2012). The earlier study considered four block design selection rules—two for choosing a subset to contain the “best” population (i.e., state with lowest mean fatality rate) and two for the “worst” population (i.e., highest mean rate) with a probability of correct selection chosen to be 0.90. Two selection rules based on normal scores resulted in selected subset sizes substantially smaller than corresponding rules based on ranks (7 vs. 16 and 3 vs. 12). For two other selection rules, the subsets chosen were very close in size (within one). A comparison is also made using state homicide rates, published in a 2022 article, with fifty states and covering eight years. The results are qualitatively the same as those obtained with the motor vehicle traffic fatality rates.

Correlation Study Between T Lymphocyte Subsets and Rheumatoid Arthritis

Objective:To study the effect of Helicobacter pylori infection on rheumatoid arthritis and T-lymphocyte subpopulations in patients with rheumatoid arthritis and to provide a new method for the treatment of rheumatoid arthritis by removing Helicobacter pylori from patients.Methods:60 patients with rheumatoid arthritis admitted to the hospital from May 2022 to May 2023 were selected for the study,and all patients underwent a 13-carbon urea breath test to detect gastric H.pylori and the test results showed that 20 cases were negative and 40 cases were positive.The 40 positive patients were divided into the treatment group(n=20)and non-treatment group(n=20)by random number table method and the treatment group was given anti-Helicobacter pylori treatment,and the non-treatment group was given maintenance rheumatoid basic treatment,comparing the anti-cyclic citrulline peptide(CCP),DS28 score,peripheral blood T-lymphocyte subsets(CD4^(+)T-lymphocytes,CD8^(+)T-lymphocytes,CD4^(+)/CD8^(+)ratio)before and after the treatment of patients by 13-carbon urea respiration test(pylori-negative group,20 patients)and those who were positive for the treatment of H pylori(pylori-positive group,40 patients).Besides,the correlation of peripheral blood T-lymphocyte subsets and disease activity between treatment and non-treatment groups in the pylori-positive group was identified together with the correlation of DS28 scores,TNF-αlevels,sedimentation and immunoglobulin,lymphocyte subsets in the pylori-positive treatment group and positive non-treatment group as well as the level of globulin,lymphocyte subsets,and peripheral blood lymphocytes before and after treatment.Results:Before treatment,CCP,DS28 score,CD8^(+)T lymphocyte level of the pylori-negative group were lower than that of the positive group,and CD4^(+)T lymphocyte and CD4^(+)/CD8^(+)ratio were higher than that of the positive group(P<0.05);after treatment,the indexes of the pylori-positive group improved,and there was no significant difference in the comparison of the indexes with those of the pylori-negative group(P>0.05);the positive treatment group had a DS28(3.19±1.02)points,positive non-treatment group DS28(5.36±1.85)points,non-treatment group DS28 score and CD4^(+)T lymphocytes,CD4^(+)/CD8^(+)negative correlation with CD8^(+)T lymphocytes showed a positive correlation(P<0.05);before the treatment,pylori-positive treatment group and non-treatment group DS28 scores,TNF-αlevels,peripheral blood T lymphocyte subpopulation levels were not significantly different(P>0.05);after treatment,DS28 score,TNF-αlevel,CD8^(+)T of the treatment group were lower than those of the non-treatment group,and CD4^(+)T lymphocytes and CD4^(+)/CD8^(+)ratio were higher than those of the non-treatment group(P<0.05).Conclusion:H.pylori affects the level of T lymphocyte subsets in patients with rheumatoid arthritis,and there is a certain correlation between the two.Removal of H.pylori can improve the level of T lymphocyte subsets,which is important for the treatment of patients with rheumatoid arthritis.

SFSDA:Secure and Flexible Subset Data Aggregation with Fault Tolerance for Smart Grid

Smart grid(SG)brings convenience to users while facing great chal-lenges in protecting personal private data.Data aggregation plays a key role in protecting personal privacy by aggregating all personal data into a single value,preventing the leakage of personal data while ensuring its availability.Recently,a flexible subset data aggregation(FSDA)scheme based on the Pail-lier homomorphic encryption was first proposed by Zhang et al.Their scheme can dynamically adjust the size of each subset and obtain the aggregated data in the corresponding subset.In this paper,firstly,an efficient attack with both theorems proving and experimentative verification is launched.We find that in a specific scenario where the encrypted data constructed by a smart meter(SM)exceeds the size of one Paillier ciphertext,the malicious fog node(FN)may use the received ciphertext to obtain the reading of the SM.Secondly,to avoid the possibility of privacy disclosure under certain circumstances,additional hash functions are added to the individual encryption process.In addition,fault tolerance is very important to aggregation schemes in practical scenarios.In most of the current schemes,once some SMs failed,then they will not work.As far as we know,there is no multi-subset aggregation scheme both supports flexible subset data aggregation and fault tolerance.Finally,we construct the first secure flexible subset data aggregation(SFSDA)scheme with fault tolerance by combining the fault tolerance method with the flexible multi-subset aggregation,where FN enables the control server(CS)to finally decrypt the aggregated ciphertext by recovering equivalent ciphertexts when some SMs fail to submit their ciphertexts.Experiments show that our SFSDA scheme keeps the efficiency in implementing a flexible multi-subset aggregation function,and only has a small delay in implementing fault-tolerant data aggregation.

An Efficient Allocation for Lung Transplantation Using Ant Colony Optimization

A relationship between lung transplant success and many features of recipients’/donors has long been studied.However,modeling a robust model of a potential impact on organ transplant success has proved challenging.In this study,a hybrid feature selection model was developed based on ant colony opti-mization(ACO)and k-nearest neighbor(kNN)classifier to investigate the rela-tionship between the most defining features of recipients/donors and lung transplant success using data from the United Network of Organ Sharing(UNOS).The proposed ACO-kNN approach explores the features space to identify the representative attributes and classify patients’functional status(i.e.,quality of life)after lung transplantation.The efficacy of the proposed model was verified using 3,684 records and 118 input features from the UNOS.The developed approach examined the reliability and validity of the lung allocation process.The results are promising regarding accuracy prediction to be 91.3%and low computational time,along with better decision capabilities,emphasizing the potential for automatic classification of the lung and other organs allocation pro-cesses.In addition,the proposed model recommends a new perspective on how medical experts and clinicians respond to uncertain and challenging lung alloca-tion strategies.Having such ACO-kNN model,a medical professional can sum-marize information through the proposed method and make decisions for the upcoming transplants to allocate the donor organ.

Unitariness in Ordered Semigroups

We introduce the concepts of unitary, almost unitary and strongly almost unitary subset of an ordered semigroup. For the notions of almost unitary and strongly almost unitary subset of an ordered semigroup, we use the notion of translational hull of an ordered semigroup. If (S,⋅,≤) is an ordered semigroup having an element e such that e ≤ e2 and U is a nonempty subset of S such that u ≤ eu, u ≤ ue for all u ∈ U, we show that U is almost unitary in S if and only if U is unitary in . Also if (S,⋅,≤) is an ordered semigroup, e ∉ S, U is a nonempty subset of S, Se:= S ∪ {e} and Ue:= U ∪ {e}, we give conditions that an (“extension” of S) ordered semigroup and the subset Ue of Se must satisfy in order for U to be almost unitary or strongly almost unitary in S (in case U is strongly almost unitary in S, then the given conditions are equivalent).

外周血CD8^(+)T淋巴细胞亚群检测对过敏性鼻炎临床疗效的评估价值

目的探讨外周血CD8^(+)T淋巴细胞亚群检测对过敏性鼻炎(AR)临床疗效的评估价值。方法前瞻性选取2021年3月~2022年2月温州市中西医结合医院耳鼻咽喉科门诊治疗的120例AR患者和40例健康志愿者,分别设为研究组和对照组,比较两组外周血CD8^(+)T细胞、CD8^(+)CD28^(+)T细胞、CD8^(+)CD28-T细胞比率;研究组接受鼻用激素联合口服抗组胺药物治疗方案,根据治疗效果分为有效组(n=107)和无效组(n=13),比较有效组和无效组治疗前CD8^(+)T细胞、CD8^(+)CD28^(+)T细胞、CD8^(+)CD28-T细胞比率;Pearson相关性分析法分析外周血CD8^(+)T淋巴细胞亚群水平与鼻部症状总评分(totalnose symptomscore,TNSS)的相关性;以受试者工作特征(ROC)曲线分析治疗前CD8^(+)T淋巴细胞亚群对AR临床疗效的预测价值。结果研究组治疗前外周血CD8^(+)CD28^(+)T细胞比率高于对照组,CD8^(+)CD28-T细胞比率低于对照组(P<0.05)。研究组总有效率为89.17%;有效组治疗前CD8^(+)CD28^(+)T细胞比率低于无效组,CD8^(+)CD28-T细胞比率高于无效组(P<0.05);Pearson相关性分析显示,CD8^(+)CD28^(+)T细胞比率与TNSS评分呈正相关(r=0.324,P=0.006),CD8^(+)CD28-T细胞比率与TNSS评分呈负相关(r=-0.541,P=0.000)。ROC曲线结果显示,CD8^(+)T细胞、CD8^(+)CD28^(+)T细胞、CD8^(+)CD28-T细胞比率评估AR治疗无效的曲线下面积(AUC)(95%CI)分别为0.647(0.555~0.732)、0.683(0.592~0.765)、0.911(0.845~0.955),其中CD8^(+)CD28-T细胞的评估效能优于CD8^(+)T细胞、CD8^(+)CD28^(+)T细胞比率(P<0.05)。结论AR患者外周血CD8^(+)CD28^(+)T细胞比率升高,CD8^(+)CD28-T细胞比率降低,CD8^(+)T细胞比率相对稳定,治疗前CD8^(+)CD28-T细胞比率可作为预测AR患者的临床疗效的参考指标。

Association of T lymphocyte subset counts with the clinical features of colorectal cancer

Background:Colorectal cancer(CRC)is a common gastrointestinal malignancy.The T lymphocyte subsets are important in the develop-ment,invasion and metastasis of tumors,including CRC.Nevertheless,limited research has explored the relationship between T cell subpopu-lations and the clinical characteristics of CRC.This study compared the T lymphocyte subsets in patients with CRC and healthy individuals,and assessed the relationship between these values and clinical characteristics.Methods:Peripheral blood was collected from 100 patients with CRC and 54 healthy individuals.The numbers of CD3^(+)T,CD4^(+)T,and CD8^(+)T lymphocytes,NK cells,and the CD4^(+)T/CD8^(+)T ratio in peripheral blood were measured using flow cytometry,and were compared between CRC patients and healthy individuals.Spearman's correlation analysis was performed to investigate the relationship between the T lymphocyte subsets in patients diagnosed with CRC and the levels of carcinoembryonic antigen(CEA)and thymidine kinase 1(TK1).Receiver operating characteristic(ROC)curves were utilized to evaluate the potential utility of the T lymphocyte counts in predicting lymph node metastasis,vas-cular infiltration,and high Ki-67 expression.Results:The CRC patients had lower counts of CD3^(+)T,CD4^(+)T,and CD8^(+)T lymphocytes compared to the healthy population(P<0.05).However,no significant differences were observed in the CD4^(+)/CD8^(+)ratio or NK cells(P>0.05).Notably,the CD3^(+)T,CD4^(+)T,and CD8^(+)T lym-phocyte counts were higher in patients with stageⅠ-Ⅱdisease,no lymph node metastasis,no vascular invasion,and low Ki-67 expression than in those with stageⅢ,lymph node metastasis,vascular invasion,and high Ki-67 expression(P<0.05).There was a negative association be-tween the CD3^(+)T,CD4^(+)T,and CD8^(+)T lymphocyte counts and CEA and TK1 levels in patients with CRC.The ROC curves demonstrated that CD3^(+)T,CD4^(+)T,and CD8^(+)T lymphocyte counts had significant predictive value for lymph node metastasis,vascular infiltration,and high Ki-67 expression.Conclusions:The peripheral blood CD3^(+)T,CD4^(+)T,and CD8^(+)T lymphocyte counts are related to the clinical traits of patients with CRC and can predict the prognosis of the disease.

SBAS-InSAR技术监测青藏高原季节性冻土形变

冻土的冻结和融化的反复交替会造成地质环境与结构的破坏,从而导致房屋和道路等地面工程建筑物的地基破裂或者塌陷,还会引起山体滑坡、洪水暴发以及冰川移动等.因此,监测冻土形变对确保冻土区工程建筑的稳定性和安全性,同时保证冻土区社会经济可持续发展具有重要的意义.目前,在冻土监测方面并没有能大面积监测冻土形变时间演化情况的有效方法,本文提出将InSAR技术中的小基线集方法(SBAS-InSAR)应用于监测冻土来获取其形变时间序列中.考虑到冻土形变呈现明显的季节性特征,本文提出利用周期形变模型来代替传统SBAS方法中的线性形变模型,从而更好地分离出高程残差和大气误差.利用ENVISAT卫星获取的21景ASAR影像图作为实验数据,采用改进的SBAS技术成功获取了青藏高原从羊八井站至当雄站铁路段冻土区的地表形变时间序列图,揭示了该冻土区从2007年到2010年的季节性形变演化情况.通过与研究地区温度变化的联合分析,发现所得到的地表形变结果与冻土的物理变化规律非常吻合,证明了SBAS-InSAR技术在冻土形变监测中具有良好的发展应用前景.

基于短基线集方法(SBAS-INSAR)技术的山区城市地面沉降监测研究——以攀枝花为例

基于合成孔径雷达干涉(interferometric synthetic aperture radar,INSAR)技术的山区城市地面沉降研究,因地形复杂、植被覆盖等因素开展较少。首次在攀枝花市,利用12景2009-2010ENVISAT ASAR数据,采用短基线集方法开展大范围、长时间监测。结果表明,攀枝花市东区地面沉降较大,沉降的平均速率高达48 mm/年;西区沉降较小,沉降平均速率10 mm/年。推测原因多为城市化建设所致地面荷载;而市东北方向的露天矿区,因连续开采所致,不同时期同一地点的地表变化,导致高相干点在此区域分散稀疏,不能表明是否有沉降现象。

腺样体肥大患儿机体免疫功能变化研究

<正>腺样体也称咽扁桃体或增殖体,属于淋巴组织,表面呈桔瓣样。正常生理情况下,儿童6~7岁时腺样体发育至最大,青春期后逐渐萎缩,成人后基本消失~[1]。腺样体位于鼻咽顶后壁处,病理性肥大易引起鼻堵、张口呼吸、耳闷、听力下降等症状,最终造成慢性鼻-鼻窦炎、阻塞性睡眠呼吸暂停低通气综合征及分泌性中耳炎等疾病,给儿童的颌面骨发育、智力发育造成严重影响。

工业CT散射校正方法的研究

在工业 CT的扫描重建过程中 ,散射效应使得重建图像出现模糊 ,各点灰度值同实际物体的吸收系数之间出现偏差 ,需要进行校正。如果采用一般的卷积方法 ,则需要迭代多次才能收敛 ,所以在校正时间上不能达到要求。采用一种结合散射模型的 Ordered Subsets Convex方法对散射效果进行校正 。

流体结构及其衍变逻辑——徐孟东《交响幻想曲》音响技术分析

徐孟东受2012年"北京现代音乐节"委约创作的《交响幻想曲》是一首为中提琴创作的大型队作品(三管编制),其音响组织新颖,结构独特,表现了徐孟东音乐创作一贯的发古思幽的人情怀。多个主题均由两个简洁的、具有五声性的双动机衍展而成。集合群在音响特征上出自然音类属G11(dia)的作用,集合衔接上采用不变子集贯连的做法,让音响具有流体运之效果。整体结构为双动机展衍多部并列结构,贯穿运用展开性变奏手法,段落之间具有渐的特点。音乐表现上,中提琴犹如一个踟蹰彷徨的行者,隔空与南宋郭沔之《潇湘水云》对谈既有云水苍茫等自然之景的描绘,也有人生感慨与超脱之言说。

Effect of combined treatment with cyclophosphamidum and allicin on neuroblastoma-bearing mice

Objective:To evaluate the efficacy of allicin combined with cyclophosphamide on neuroblastoma(NB)-bearing mice and explore the immunological mechanism in it.Methods:A total of 30NB-bearing mice were equally randomized into model group,cyclophosphamide group and combined therapy group,10 nudemice were set as normal saline(NS) group.Cyclophosphamide group and combined therapy group were weekly injected with 60 mg/kg cyclophosphamide for four weeks;besides,combined therapy group was given with allicin(10 mg/kg/d) by gastric perfusion for 4 weeks;model group and NS group were given with the same volume of NS.Serum VEGF content was detected by ELISA pre-treating(0 d) and on the 3rd d.14 th d and 28 th d;on29th d,all mice were sacrificed and the tumor,liver,spleen and thymic tissues were weighted.Tumors were made into paraffin section for detecting tumor cell apoptosis and proliferation by TUNEI.and BrdU method,respectively.Survival curves were drawn by Kaplan-Meier method.Results:After treatment,both treatment groups relieved on viscera indexes,VEGF level,T cell subsets distribution and tumor growth and each index of combined therapy group was better than cyclophosphamide group(P【0.05 or 0.01);only combined therapy group could significantly increase the lifetime of NB-bearing mice(χ~2=5.667,P=0.017).Conclusions:Allicin can improve T cell subsets distribution and inhibit VEGF expression through its immunomodulatory activity,thereby improve the efficiency on NB in coordination with cyclophosphamide.

VX-765 reduces neuroinflammation after spinal cord injury in mice

Inflammation is a major cause of neuronal injury after spinal cord injury. We hypothesized that inhibiting caspase-1 activation may reduce neuroinflammation after spinal cord injury, thus producing a protective effect in the injured spinal cord. A mouse model of T9 contusive spinal cord injury was established using an Infinite Horizon Impactor, and VX-765, a selective inhibitor of caspase-1, was administered for 7 successive days after spinal cord injury. The results showed that:(1) VX-765 inhibited spinal cord injury-induced caspase-1 activation and interleukin-1β and interleukin-18 secretion.(2) After spinal cord injury, an increase in M1 cells mainly came from local microglia rather than infiltrating macrophages.(3) Pro-inflammatory Th1 Th17 cells were predominant in the Th subsets. VX-765 suppressed total macrophage infiltration, M1 macrophages/microglia, Th1 and Th1 Th17 subset differentiation, and cytotoxic T cells activation;increased M2 microglia;and promoted Th2 and Treg differentiation.(4) VX-765 reduced the fibrotic area, promoted white matter myelination, alleviated motor neuron injury, and improved functional recovery. These findings suggest that VX-765 can reduce neuroinflammation and improve nerve function recovery after spinal cord injury by inhibiting caspase-1/interleukin-1β/interleukin-18. This may be a potential strategy for treating spinal cord injury. This study was approved by the Animal Care Ethics Committee of Bengbu Medical College(approval No. 2017-037) on February 23, 2017.

Effects of Radiofrequency Ablation on Lymphocyte Subsets and Type 1/Type 2 T Cell Subpopulations in Patients with Hepatocellular Carcinoma

Objective： To evaluate whether radiofrequency ablation （RFA） might have an influence on immune status in hepatocellular carcinoma （HCC） patients. Methods： We measured the T lymphocytes, B lymphocyte and NK cells, and determined the population of Thl, Th2, Tcl and Tc2 of peripheral blood samples taken from 26 HCC patients before and after RFA. Results： The proportion of Typel cells （Thl and Tcl） and NK cells were significantly increased after RFA, especially in patients of the following subgroups： male, age〉55 years, pathological grade Ⅰ-Ⅱ tumor, clinical stage Ⅰ-Ⅱ or Child-Pugh A and B. Conclusion： TypeⅠ cells and NK cells in HCC patients were increased in a short period after RFA.

Lymphocyte Subsets and Sister-chromatid Exchanges in the Students Exposed to Formaldehyde Vapor

The present report evaluates the effects of formaldehyde (FA) exposure on peripheral lymphocytes by using heth genetic and immunological parameters. Twenty-three non-smoking students in the study had inhalation exposure to 0.508 ±0. 299 mg/m3 of FA for a Period of 8 weeks (3h × 3 times each week) during anatomy classes. As for composition of lymphocyte subsets after FA exposare,significant increase was found in the percentage of CD19(B cells), while sighficant decrease was observed in CD3(total T cells), CD4(T helper-inducer cells), and CD8(T cytotoxic-suppressior cells) with a P<0 .01. Increase in the ratio of T-helper-inducer cells to T-cytotoxic-suppressor cells (T4 / T8) was also observed with statistical sighcance after exposure (P < 0.001). In the meanwhile,no significant difference (P > 0 .05) was reported between lymphocyte prolifendion rate and sisterchromatid exchange (SCE) at the exposure level and duration. It is suggested that the lymphocyte subsets may be most susceptible to the effects of FA, though a single immunological endpoint is rarely related with pathophysiological interpretation.

Distribution of natural killer cells and T-lymphocyte subsets in peripheral blood,gallbladder cancer and surrounding tissue

BACKGROUND: The patient with malignant tumor always show immunologic function drawback and ingravescent with tumor development, especially in the aspect of cell-mediated immunity. This study was undertaken to define the relationship between the immune function of local cells and cancer development by investigating the distribution of natural killer (NK) cells and T-lymphocyte subsets in peripheral blood, the cancer tissue and the tissue surrounding gallbladder carcinoma. METHODS: The numbers of CD4(+) and CD8(+) T-lymphocytes and NK cells were measured by flow cytometry in samples taken from gallbladder cancer tissue, the surrounding tissues and peripheral blood of 38 patients, and compared with the numbers in the peripheral blood and gallbladder tissue of 30 patients with cholecystitis as controls. RESULTS: The numbers of CD4(+) and CD8(+) T-cells and NK cells in gallbladder cancer tissues were significantly higher than those in the surrounding tissue and gallbladder with gallstone. However, the ratio of CD4(+)/CD8(+) was lower in the cancer tissue than that in the surrounding tissue and tissue from gallbladders with gallstones. The distribution of CD4(+) and CD8(+) T-cells and NK cells in mucous membrane of cholecystitis gallbladder and that in the tissue surrounding gallbladder cancer were significantly different. CONCLUSIONS: Disproportionate and imbalanced distribution of NK cells and subsets of T-lymphocytes occurs in the mucous membrane proper of gallbladder cancer and surrounding tissue. Although gallbladder cancer tissue has higher expressions of CD4(+), CD8(+) and NK cells, the immune function is low or in an inhibited state. In gallbladder cancer immunization therapy, local cellular immunological function should be enhanced and the protective barrier improved.

Review of the SBAS InSAR Time-series algorithms, applications, and challenges

In the past 30 years,the small baseline subset(SBAS)InSAR time-series technique has emerged as an essential tool for measuring slow surface displacement and estimating geophysical parameters.Because of its ability to monitor large-scale deformation with millimeter accuracy,the SBAS method has been widely used in various geodetic fields,such as ground subsidence,landslides,and seismic activity.The obtained long-term time-series cumulative deformation is vital for studying the deformation mecha-nism.This article reviews the algorithms,applications,and challenges of the SBAS method.First,we recall the fundamental principle and analyze the shortcomings of the traditional SBAS algorithm,which provides a basic framework for the following improved time series methods.Second,we classify the current improved SBAS techniques from different perspectives:solving the ill-posed equation,increasing the density of high-coherence points,improving the accuracy of monitoring deformation and measuring the multi-dimensional deformation.Third,we summarize the application of the SBAS method in monitoring ground subsidence,permafrost degradation,glacier movement,volcanic activity,landslides,and seismic activity.Finally,we discuss the difficulties faced by the SBAS method and explore its future development direction.