Objective To study the effects of intranigral injection of different doses of CuSO45H2O on dopaminergic neuron in the nigrostriatal system of rats. Methods Wistar rats were divided into four groups, including control ...Objective To study the effects of intranigral injection of different doses of CuSO45H2O on dopaminergic neuron in the nigrostriatal system of rats. Methods Wistar rats were divided into four groups, including control group, 10 nmol, 50 nmol and 200 nmol copper injected into left substantia nigra （SN） groups. Seven days after the intranigral injection of copper, dopamine （DA） contents in the striatum （Str） were measured by high performance lipid chromotophotography （HPLC）; the density of tyrosine hydroxylase （TH） positive axons in the Str was measured by TH staining method; TH and Caspase-3 mRNA expression in the SN were measured by semi-quantitative RT-PCR. We detected the activity of superoxide dismutase （SOD） in the lesioned midbrain of rats using biochemical methods. Results DA and its metabolites contents had no significant difference between control group and low dose （10 nmol） copper group. But from 50 nmol copper group, DA contents in the lesioned sides were reduced with the increase in the copper doses injected, showing a significant linear correlation （F = 34.16, P 〈 0.01）. In the 50 nmol copper group, TH positive axons in the Str decreased compared with those of the control and unlesioned sides （F = 121.9, P 〈 0.01）. In the 50 nmol copper group, TH mRNA expression decreased （t =3.12, P 〈 0.01） while Caspase-3 mRNA expression increased （t =8.96, P 〈 0.01） in the SN compared with the control. SOD activity decreased in the midbmin of rots treated with 50 nmol copper compared with that of the control （t = 2.33, P〈0.01）. Conclusion Copper could induce damage of dopaminergic neurons in the SN of rats through destroying antioxidant defenses and promoting apoptosis.展开更多
将老年(26～27个月)及成年(3～4个月)大鼠脑切片用乙醛酸诱发单胺类荧光法,显示其黑质多巴胺(DA)。形态学观察见到,成年大鼠黑质神经元胞体充满强蓝绿色 DA 荧光,突起近端亦显荧光;而老年大鼠黑质神经元胞体蓝绿色 DA 荧光明显减弱,代...将老年(26～27个月)及成年(3～4个月)大鼠脑切片用乙醛酸诱发单胺类荧光法,显示其黑质多巴胺(DA)。形态学观察见到,成年大鼠黑质神经元胞体充满强蓝绿色 DA 荧光,突起近端亦显荧光;而老年大鼠黑质神经元胞体蓝绿色 DA 荧光明显减弱,代之以较强的金黄色脂褐素荧光,DA 荧光细胞数量减少。显微荧光光度术对神经元 DA 荧光的定性和定量分析表明,DA 荧光发射光谱峰值为490 nm;脂褐素荧光发射光谱峰值为550 nm。成年组 DA 荧光强度((?)±S_(?))为55.813±3.032,老年组 DA 荧光强度((?)±S_(?))为28.905±2.746,P<0.001,说明老年大鼠黑质DA 浓度较成年组显著降低。本文将所得结果与已有的研究进行了对比分析,并对 DA 降低的可能影响进行了初步讨论。展开更多
文摘Objective To study the effects of intranigral injection of different doses of CuSO45H2O on dopaminergic neuron in the nigrostriatal system of rats. Methods Wistar rats were divided into four groups, including control group, 10 nmol, 50 nmol and 200 nmol copper injected into left substantia nigra （SN） groups. Seven days after the intranigral injection of copper, dopamine （DA） contents in the striatum （Str） were measured by high performance lipid chromotophotography （HPLC）; the density of tyrosine hydroxylase （TH） positive axons in the Str was measured by TH staining method; TH and Caspase-3 mRNA expression in the SN were measured by semi-quantitative RT-PCR. We detected the activity of superoxide dismutase （SOD） in the lesioned midbrain of rats using biochemical methods. Results DA and its metabolites contents had no significant difference between control group and low dose （10 nmol） copper group. But from 50 nmol copper group, DA contents in the lesioned sides were reduced with the increase in the copper doses injected, showing a significant linear correlation （F = 34.16, P 〈 0.01）. In the 50 nmol copper group, TH positive axons in the Str decreased compared with those of the control and unlesioned sides （F = 121.9, P 〈 0.01）. In the 50 nmol copper group, TH mRNA expression decreased （t =3.12, P 〈 0.01） while Caspase-3 mRNA expression increased （t =8.96, P 〈 0.01） in the SN compared with the control. SOD activity decreased in the midbmin of rots treated with 50 nmol copper compared with that of the control （t = 2.33, P〈0.01）. Conclusion Copper could induce damage of dopaminergic neurons in the SN of rats through destroying antioxidant defenses and promoting apoptosis.
文摘将老年(26～27个月)及成年(3～4个月)大鼠脑切片用乙醛酸诱发单胺类荧光法,显示其黑质多巴胺(DA)。形态学观察见到,成年大鼠黑质神经元胞体充满强蓝绿色 DA 荧光,突起近端亦显荧光;而老年大鼠黑质神经元胞体蓝绿色 DA 荧光明显减弱,代之以较强的金黄色脂褐素荧光,DA 荧光细胞数量减少。显微荧光光度术对神经元 DA 荧光的定性和定量分析表明,DA 荧光发射光谱峰值为490 nm;脂褐素荧光发射光谱峰值为550 nm。成年组 DA 荧光强度((?)±S_(?))为55.813±3.032,老年组 DA 荧光强度((?)±S_(?))为28.905±2.746,P<0.001,说明老年大鼠黑质DA 浓度较成年组显著降低。本文将所得结果与已有的研究进行了对比分析,并对 DA 降低的可能影响进行了初步讨论。
