Combined application of chemical fertilizers with organic amendments was recommended as a strategy for improving yield,soil carbon storage,and nutrient use efficiency.However,how the long-term substitution of chemical...Combined application of chemical fertilizers with organic amendments was recommended as a strategy for improving yield,soil carbon storage,and nutrient use efficiency.However,how the long-term substitution of chemical fertilizer with organic manure affects rice yield,carbon sequestration rate(CSR),and nitrogen use efficiency(NUE)while ensuring environmental safety remains unclear.This study assessed the long-term effect of substituting chemical fertilizer with organic manure on rice yield,CSR,and NUE.It also determined the optimum substitution ratio in the acidic soil of southern China.The treatments were:(i)NPK0,unfertilized control;(ii)NPK1,100%chemical nitrogen,phosphorus,and potassium fertilizer;(iii)NPKM1,70%chemical NPK fertilizer and 30%organic manure;(iv)NPKM2,50%chemical NPK fertilizer and 50%organic manure;and(v)NPKM3,30%chemical NPK fertilizer and 70%organic manure.Milk vetch and pig manure were sources of manure for early and late rice seasons,respectively.The result showed that SOC content was higher in NPKM1,NPKM2,and NPKM3 treatments than in NPK0 and NPK1 treatments.The carbon sequestration rate increased by 140,160,and 280%under NPKM1,NPKM2,and NPKM3 treatments,respectively,compared to NPK1 treatment.Grain yield was 86.1,93.1,93.6,and 96.5%higher under NPK1,NPKM1,NPKM2,and NPKM3 treatments,respectively,compared to NPK0 treatment.The NUE in NPKM1,NPKM2,and NPKM3 treatments was higher as compared to NPK1 treatment for both rice seasons.Redundancy analysis revealed close positive relationships of CSR with C input,total N,soil C:N ratio,catalase,and humic acids,whereas NUE was closely related to grain yield,grain N content,and phenol oxidase.Furthermore,CSR and NUE negatively correlated with humin acid and soil C:P and N:P ratios.The technique for order of preference by similarity to ideal solution(TOPSIS)showed that NPKM3 treatment was the optimum strategy for improving CSR and NUE.Therefore,substituting 70%of chemical fertilizer with organic manure could be the best management option for increasing CSR and NUE in the paddy fields of southern China.展开更多
Water-saving irrigation strategies can successfully alleviate methane emissions from rice fields,but significantly stimulate nitrous oxide(N_(2)O)emissions because of variations in soil oxygen level and redox potentia...Water-saving irrigation strategies can successfully alleviate methane emissions from rice fields,but significantly stimulate nitrous oxide(N_(2)O)emissions because of variations in soil oxygen level and redox potential.However,the relationship linking soil N_(2)O emissions to nitrogen functional genes during various fertilization treatments in water-saving paddy fields has rarely been investigated.Furthermore,the mitigation potential of organic fertilizer substitution on N_(2)O emissions and the microbial mechanism in rice fields must be further elucidated.Our study examined how soil N_(2)O emissions were affected by related functional microorganisms(ammonia-oxidizing archaea(AOA),ammonia-oxidizing bacteria(AOB),nirS,nirK and nosZ)to various fertilization treatments in a rice field in southeast China over two years.In this study,three fertilization regimes were applied to rice cultivation:a no nitrogen(N)(Control),an inorganic N(Ni),and an inorganic N with partial N substitution with organic manure(N_(i)+N_(o)).Over two rice-growing seasons,cumulative N_(2)O emissions averaged 0.47,4.62 and 4.08 kg ha^(−1)for the Control,Ni and N_(i)+N_(o)treatments,respectively.In comparison to the Ni treatment,the N_(i)+N_(o)fertilization regime considerably reduced soil N_(2)O emissions by 11.6%while maintaining rice yield,with a lower N_(2)O emission factor(EF)from fertilizer N of 0.95%.Nitrogen fertilization considerably raised the AOB,nirS,nirK and nosZ gene abundances,in comparison to the Control treatment.Moreover,the substitution of organic manure for inorganic N fertilizer significantly decreased AOB and nirS gene abundances and increased nosZ gene abundance.The AOB responded to N fertilization more sensitively than the AOA.Total N_(2)O emissions significantly correlated positively with AOB and nirS gene abundances while having a negative correlation with nosZ gene abundance and the nosZ/nirS ratio across N-fertilized plots.In summary,we conclude that organic manure substitution for inorganic N fertilizer decreased soil N_(2)O emissions primarily by changing the soil NO_(3)^(−)-N,pH and DOC levels,thus inhibiting the activities of ammonia oxidation in nitrification and nitrite reduction in denitrification,and strengthening N_(2)O reduction in denitrification from water-saving rice paddies.展开更多
[Objective] The aim was to explore recycling utilization of manure of dairy cattle through returning of manures into fields. [Method] Effects of dairy cattle ma- nure and chemical fertilizer on fertility of soils grow...[Objective] The aim was to explore recycling utilization of manure of dairy cattle through returning of manures into fields. [Method] Effects of dairy cattle ma- nure and chemical fertilizer on fertility of soils grown with Cichorium intybus were in- vestigated through a pot experiment. [Result] After manure of dairy cattle was ap- plied, it can be concluded that organic matter, total N, total P, alkali-hydrolyzable ni- trogen, available P, activities of urease and invertase in soils increased by 0.14-1.28 times, 43.8%-79.7%, 17.4%-30.8%, 147%-188%, 7 times, 17.2%-38.5%, and 1.36%- 3.34%, respectively. Furthermore, organic matter, total N. urease and invertase activi- ties in group of M7F3 increased most; total P and available P achieved the best in group of M3F7. These indicated that the applied manures of dairy cattle would maintain and improve soil fertility, providing better soils for Cichorium intybus. [Conclusion] The research provides reference for recycling of cattle manures and construction of ecological cyclical pattern of "grass planting-cattle breeding-methane fermentation-returning of manures into fields".展开更多
To evaluate the effect of organic manure application with chemical fertilizers on rice yield and soil fertility under long-term double-rice cropping system, a six year field experiment was conducted continually in the...To evaluate the effect of organic manure application with chemical fertilizers on rice yield and soil fertility under long-term double-rice cropping system, a six year field experiment was conducted continually in the paddy soil derived from Quaternary red clay in Hunan Province of southern China. Four different treatments, i.e., no nitrogen with chemical P and K (PK), swine manure only (M), N, P and K chemical fertilizers only (NPK), and half chemical fertilizers combined with half swine manure (NPKM) with four replications were included. Each N, P and K application rate was the same at all the treatments (except the N application rate at PK) and N application rate was 150 kg N ha^-1. All fertilizers were applied to soil tillage layer with once application as baseal fertilizers. The nutrients uptake rate, grain yield, nitrogen use efficiency, and soil organic matter content at each treatment were investigated. The NPKM treatment achieved the highest mean annual yield of 12.2 t ha^-1 (68% higher than that of PK). Higher dry matter accumulation and nutrients absorption were observed during the middle-late growth period in the NPKM treatment, with higher panicle number per unit and filled-grain number per panicle. Its average nitrogen use efficiency was 36.3% and soil organic matter increased by 18.5% during the experimental period in the NPKM treatment, which were significantly higher than those in the NPK treatment. Organic manure application with chemical fertilizers increased the yield and nitrogen use efficiency of rice, reduced the risk of environmental pollution and improved soil fertility greatly. It could be a good practical technique that protects the environment and raises the rice yield in this region.展开更多
A long term fertilization experiment was carried out in an experimental field in Lyczyn near Warsaw, Poland. Application ofmineral fertilizers, especially N fertilizers with and without farmyard manure accelerated th...A long term fertilization experiment was carried out in an experimental field in Lyczyn near Warsaw, Poland. Application ofmineral fertilizers, especially N fertilizers with and without farmyard manure accelerated the acidification process of the soil. Application of 1.6 t CaO ha -1 every four years was essential to maintenance of the soil pH KCl at 5.5~6.6 and base saturation degree above 60%. Application of 50 t farmyard manure ha -1 every 4 years, which contained 46 kg P and 240 kg K, was sufficient to maintain both the K and P fertility of the soil. Besides, it was beneficial to alleviating soil acidification. As a result of long term unbalanced fertilization, yield responses to N, P and K fertilizers increased significantly with time. The efficiency of N from farmyard manure was found to be comparable to that of N fertilizer during 1988~1991.展开更多
Synthetic nitrogen(N)fertilizer has made a great contribution to the improvement of soil fertility and productivity,but excessive application of synthetic N fertilizer may cause agroecosystem risks,such as soil acidif...Synthetic nitrogen(N)fertilizer has made a great contribution to the improvement of soil fertility and productivity,but excessive application of synthetic N fertilizer may cause agroecosystem risks,such as soil acidification,groundwater contamination and biodiversity reduction.Meanwhile,organic substitution has received increasing attention for its ecologically and environmentally friendly and productivity benefits.However,the linkages between manure substitution,crop yield and the underlying microbial mechanisms remain uncertain.To bridge this gap,a three-year field experiment was conducted with five fertilization regimes:i)Control,non-fertilization;CF,conventional synthetic fertilizer application;CF_(1/2)M_(1/2),1/2 N input via synthetic fertilizer and 1/2 N input via manure;CF_(1/4)M_(3/4),1/4 N input synthetic fertilizer and 3/4 N input via manure;M,manure application.All fertilization treatments were designed to have equal N input.Our results showed that all manure substituted treatments achieved high soil fertility indexes(SFI)and productivities by increasing the soil organic carbon(SOC),total N(TN)and available phosphorus(AP)concentrations,and by altering the bacterial community diversity and composition compared with CF.SOC,AP,and the soil C:N ratio were mainly responsible for microbial community variations.The co-occurrence network revealed that SOC and AP had strong positive associations with Rhodospirillales and Burkholderiales,while TN and C:N ratio had positive and negative associations with Micromonosporaceae,respectively.These specific taxa are implicated in soil macroelement turnover.Random Forest analysis predicted that both biotic(bacterial composition and Micromonosporaceae)and abiotic(AP,SOC,SFI,and TN)factors had significant effects on crop yield.The present work strengthens our understanding of the effects of manure substitution on crop yield and provides theoretical support for optimizing fertilization strategies.展开更多
Soil biotic communities play vital roles in enhancing soil nutrient cycling and soil fertility.Long-term excessive nitrogen(N)application is disadvantageous to the stability of soil food webs and affects arable soil h...Soil biotic communities play vital roles in enhancing soil nutrient cycling and soil fertility.Long-term excessive nitrogen(N)application is disadvantageous to the stability of soil food webs and affects arable soil health and sustainable utilization.Proper organic substitution is essential to improve soil health and alleviate the disadvantages of excessive chemical fertilization.However,the biological effects of various organic amendments on soil micro-food webs are poorly understood.In order to explore the effects of various organic amendments including stover,biochar and manure on soil micro-food webs(microbial and nematode communities),a field plot experiment with maize having five treatments viz.,100%urea(100%N),70%urea(70%N),70%urea plus stover(Stover),70%urea plus cattle manure(Manure)and 70%urea plus biochar(Biochar)was conducted.Manure treatment increased the carbon(C)to N use efficiency of soil microbes,which contributed to the retention of soil C,while Biochar treatment elevated soil organic C(SOC)and soil p H.Additionally,Biochar treatment mitigated the negative effects of soil acidification on the soil micro-food web and reduced the abundance of plant parasites.Overall,the biological effect of organic amendments was distinguished from chemical fertilization(100%N and 70%N)through principal co-ordinates analysis.Negative relationships among soil properties,microbial and nematode biomass in the 100%N treatment were diminished in treatments where chemical fertilizer was reduced.The bottom-up effects on soil food webs were observed in organic substitution treatments.In conclusion,organic amendments improved soil fertility by regulating soil microbial and nematode communities in the cropland ecosystem,alleviated the negative effects of chemical fertilizer on the micro-food webs and controlled the trophic cascades among soil biota.展开更多
The field experiment cultivating wheat during the winter season from November 2014 to March 2015 was conducted in the “North Eastern Barind Tract Soils” at Kushadaha, Nawabganj, Dinajpur in Bangladesh. The wheat var...The field experiment cultivating wheat during the winter season from November 2014 to March 2015 was conducted in the “North Eastern Barind Tract Soils” at Kushadaha, Nawabganj, Dinajpur in Bangladesh. The wheat variety BARI Gom 26 was selected in focusing to evaluate the effects of organic amendments using poultry manure (PM) and cow dung (CD) on the growth, yield and nutrient uptake by the plant. The texture of the field soil was clay with acidic nature (pH 5.61). The trials comprise three treatments following a randomized complete block design (RCBD) replicated thrice. Results showed that significantly greater plant height, number of tillers per hill and straw yield were 98.10 cm, 3.66 and 5425 kg/ha respectively in receiving the treatment T<sub>2</sub> than T<sub>3</sub> (95.66 cm, 3.38 and 4483 kg/ha) and T<sub>1</sub> (control). Spike length, 1000 grain weight and grain yield were 9.23 cm, 39.81 kg/ha and 3100 kg/ha respectively also higher in T<sub>2</sub> treatment than T<sub>3</sub> (8.76 cm, 38.51 kg/ha, 3091 kg/ha) with no statistical differences among them. Therefore, the treatment T<sub>2</sub> (PM) comprised of poultry manure and NPK exhibited as the best treatment for producing the highest in all growth and yield parameters of wheat. In wheat grain, the content of N, P, K, S, Mg, Zn and B ranged from 1.080% to 1.380% N, 0.390% to 0.398% P, 0.780% to 0.840% K, 0.079% to 0.111% S, 0.181% to 0.187% Mg, 25.56 to 29.77 ppm Zn and 10.12 to 12.54 ppm B. Similarly in straw, nutrients content ranged from 0.220 to 0.300% N, 0.045% to 0.074% P, 0.970% to 1.250% K, 0.152% to 0.191% S, 0.097% to 0.101% Mg, 10.78 to 13.23 ppm Zn and 27.98 to 2989 ppm B. Therefore, organic amendment with 5-ton cow dung (CD) ha<sup>-1</sup> with a recommended dose of NPK significantly increased the content of N, S, Mg, Zn and B in the grain and straw of wheat. Alternatively, the poultry manure treatment with NPK significantly increased the content of P and K in the grain and straw of wheat. However, results revealed that the treatments T<sub>2</sub> and T<sub>3</sub> comprising a recommended dose of NPK with poultry manure and cow dung respectively could increase the content of N, P, K, S, Mg, Zn and B in wheat. The overall results expressed that the poultry manure treatment with NPK (T<sub>2</sub>) exposed as superior for producing the top growth and yield attributes of wheat in the studied area.展开更多
Substituting chemical fertilizers with manure is an important method for efficient nutrient management in rice cropping systems of China.Labile nitrogen(N)is the most active component of the soil N pool and plays an e...Substituting chemical fertilizers with manure is an important method for efficient nutrient management in rice cropping systems of China.Labile nitrogen(N)is the most active component of the soil N pool and plays an essential role in soil fertility.However,the effects of manure substitution on soil labile N in rice cropping systems and their relationships with soil properties,fertilization practices,and climatic conditions remain unclear and should be systematically quantified.Here,we investigated rice grain yield and four types of soil labile N that have been widely reported,including available nitrogen(AN),ammonium nitrogen(NH_(4)^(+)-N),nitrate nitrogen(NO_(3)^(−)-N),and microbial biomass nitrogen(MBN).We reviewed 187 published articles and performed a meta-analysis to quantify the effects of manure substitution on yield and soil labile N.The results showed that manure substitution increased AN,MBN,NH+4-N,and NO−3-N by 11.3%,38.5%,5.9%,and 8.1%,respectively.Partial substitution significantly increased the yield by 1.4%–5.9%,but full substitution significantly decreased the yield by 2.9%.The positive effects of manure substitution on yield and AN were stronger with long-term fertilization.The differences in responses varied across specific manure types,N application rates,soil properties,and climatic factors.In conclusion,manure substitution can increase soil labile N and is regarded as an efficient strategy for improving soil N fertility and a recommended measure for applying both chemical and organic fertilizers in rice systems.This study provides evidence of the effects of manure substitution on yield enhancement by increasing soil labile N.展开更多
Increased food demand from the rapidly growing human population has caused intensive land transition from desert to farmland in arid regions of northwest China. In this developing ecosystem, the optimized fertilizatio...Increased food demand from the rapidly growing human population has caused intensive land transition from desert to farmland in arid regions of northwest China. In this developing ecosystem, the optimized fertilization strategies are becoming an urgent need for sustainable crop productivity, efficient resources use, together with the delivery of ecosystems services including soil carbon(C) and nitrogen(N) accumulation. Through a 7-year field experiment with 9 fertilization treatments in a newly cultivated farmland, we tested whether different fertilizations had significant influences on soil C and N accumulation in this developing ecosystem, and also investigated possible mechanisms for this influence. The results showed that applying organic manure in cultivated farmland significantly increased the soil C and N accumulation rates; this influence was greater when it was combined with chemical fertilizer, accumulating 2.01 t C and 0.11 t N ha^(–1) yr^(–1) in the most successful fertilization treatment. These high rates of C and N accumulation were found associated with increased input of C and N, although the relationship between the N accumulation rate and N input was not significant. The improved soil physical properties was observed under only organic manure and integrated fertilization treatments, and the significant relationship between soil C or N and soil physical properties were also found in this study. The results suggest that in newly cultivated farmland, long term organic manure and integrated fertilization can yield significant benefits for soil C and N accumulation, and deliver additional influence on physical properties.展开更多
Background: Watermelon is a very important crop because it has many nutritional and economical values. This crop is known to human for ages and has been cultivated for centuries in many Middle Eastern countries includ...Background: Watermelon is a very important crop because it has many nutritional and economical values. This crop is known to human for ages and has been cultivated for centuries in many Middle Eastern countries including Syria. Several types of fertilizers are being used in order to increase the productivity of this crop but some fertilizers have shown to have an adverse effect on environment. Therefore, the aim of this study was to examine the effects of several types of organic fertilizers (cow, sheep, poultry and pigeon manure) and to compare their effects on growth, productivity and quality parameters with chemical fertilizers (NPK) and control group. Materials and Methods: Two local cultivars spherical and cylindrical (Audrey and Shapah) were used to examine the effects of cow manure (8 m/Donum), Sheep manure (6 m/Donum), poultry manure (3 m/Donum) and pigeons (2 m/Donum) and were compared with chemical fertilizer (N 20:P 40:K 25). Number of fruits on each vine, length of each vine (cm), fruit weight (kg), and estimated yield (kg/Donum) were measured and recorded some quality parameters such as rind thickness (cm) and heart color as well. Results: Cows manure was proved to be superior to other types of fertilizers (organic and inorganic) in many traits but chemical fertilizer gave the highest yield (kg/Donum) in Audrey cultivar but in Shapah cultivar there was no significant difference in productivity between chemical fertilizer compared with control group. Conclusion: Using organic fertilizers to cultivate watermelon does affect quality of watermelon and the productivity was closely related to chemical fertilizer. On the other hand, organic fertilizer can reduce the harmful effects of chemical fertilizers on environment and human health alike.展开更多
Burning crop residues and excessive use of chemical fertilizers results in an enormous waste of bio- logical resources, which further weakens the potential capacity of the agro-ecosystem as a carbon sink. To explore t...Burning crop residues and excessive use of chemical fertilizers results in an enormous waste of bio- logical resources, which further weakens the potential capacity of the agro-ecosystem as a carbon sink. To explore the potential of farmlands acting as a carbon sink without yield losses, we conducted an experiment on a temperate eco-farm in eastern rural China. Crop residues were applied to cattle feed, and the composted cattle manure was returned to cropland with a winter wheat and maize rotation. Four different proportions of fertilizers were designed: 100 % cattle manure, 100 % mineral nitrogen, 75 % cattle manure plus 25 % mineral nitrogen, and 50 % cattle manure plus 50 % mineral nitrogen. Crop yield and greenhouse gas (GHG) emissions were carefully calculated according to the Intergovernmental Panel on Climate Change (IPCC) Guidelines for National Green- house Gas Inventories 2006. Our results showed that replacing chemical fertilizer with organic manure signifi- cantly decreased the emission of GHGs. Yields of wheat and corn also increased as the soil fertility was improved by the application of cattle manure. Totally replacing chemical fertilizer with organic manure decreased GHG emissions, which reversed the agriculture ecosystem from a carbon source (+2.7 t CO_2-eq. hm-2 year-1) to a carbon sink (-8.8 t CO_2-eq. hm^-2 year^-1). Our findings provide useful insights for improving agricultural ecosystems under global change scenarios.展开更多
基金supported by the National Natural Science Foundation of China (41671301)the National Key Research and Development Program of China (2016YFD0300901)the Central Public-interest Scientific Institution Basal Research Fund, China (GY2022-13-5, G2022-02-2, G2022-02-3 and G2022-02-10)
文摘Combined application of chemical fertilizers with organic amendments was recommended as a strategy for improving yield,soil carbon storage,and nutrient use efficiency.However,how the long-term substitution of chemical fertilizer with organic manure affects rice yield,carbon sequestration rate(CSR),and nitrogen use efficiency(NUE)while ensuring environmental safety remains unclear.This study assessed the long-term effect of substituting chemical fertilizer with organic manure on rice yield,CSR,and NUE.It also determined the optimum substitution ratio in the acidic soil of southern China.The treatments were:(i)NPK0,unfertilized control;(ii)NPK1,100%chemical nitrogen,phosphorus,and potassium fertilizer;(iii)NPKM1,70%chemical NPK fertilizer and 30%organic manure;(iv)NPKM2,50%chemical NPK fertilizer and 50%organic manure;and(v)NPKM3,30%chemical NPK fertilizer and 70%organic manure.Milk vetch and pig manure were sources of manure for early and late rice seasons,respectively.The result showed that SOC content was higher in NPKM1,NPKM2,and NPKM3 treatments than in NPK0 and NPK1 treatments.The carbon sequestration rate increased by 140,160,and 280%under NPKM1,NPKM2,and NPKM3 treatments,respectively,compared to NPK1 treatment.Grain yield was 86.1,93.1,93.6,and 96.5%higher under NPK1,NPKM1,NPKM2,and NPKM3 treatments,respectively,compared to NPK0 treatment.The NUE in NPKM1,NPKM2,and NPKM3 treatments was higher as compared to NPK1 treatment for both rice seasons.Redundancy analysis revealed close positive relationships of CSR with C input,total N,soil C:N ratio,catalase,and humic acids,whereas NUE was closely related to grain yield,grain N content,and phenol oxidase.Furthermore,CSR and NUE negatively correlated with humin acid and soil C:P and N:P ratios.The technique for order of preference by similarity to ideal solution(TOPSIS)showed that NPKM3 treatment was the optimum strategy for improving CSR and NUE.Therefore,substituting 70%of chemical fertilizer with organic manure could be the best management option for increasing CSR and NUE in the paddy fields of southern China.
基金supported by the National Key Research and Development Program of China(2022YFD2300300)the National Natural Science Foundation of China(41907072)+1 种基金the Scientific Research Foundation of Zhejiang A&F University,China(2022LFR003)the Jiangsu Agriculture Science and Technology Innovation Fund,China(CX(21)3007).
文摘Water-saving irrigation strategies can successfully alleviate methane emissions from rice fields,but significantly stimulate nitrous oxide(N_(2)O)emissions because of variations in soil oxygen level and redox potential.However,the relationship linking soil N_(2)O emissions to nitrogen functional genes during various fertilization treatments in water-saving paddy fields has rarely been investigated.Furthermore,the mitigation potential of organic fertilizer substitution on N_(2)O emissions and the microbial mechanism in rice fields must be further elucidated.Our study examined how soil N_(2)O emissions were affected by related functional microorganisms(ammonia-oxidizing archaea(AOA),ammonia-oxidizing bacteria(AOB),nirS,nirK and nosZ)to various fertilization treatments in a rice field in southeast China over two years.In this study,three fertilization regimes were applied to rice cultivation:a no nitrogen(N)(Control),an inorganic N(Ni),and an inorganic N with partial N substitution with organic manure(N_(i)+N_(o)).Over two rice-growing seasons,cumulative N_(2)O emissions averaged 0.47,4.62 and 4.08 kg ha^(−1)for the Control,Ni and N_(i)+N_(o)treatments,respectively.In comparison to the Ni treatment,the N_(i)+N_(o)fertilization regime considerably reduced soil N_(2)O emissions by 11.6%while maintaining rice yield,with a lower N_(2)O emission factor(EF)from fertilizer N of 0.95%.Nitrogen fertilization considerably raised the AOB,nirS,nirK and nosZ gene abundances,in comparison to the Control treatment.Moreover,the substitution of organic manure for inorganic N fertilizer significantly decreased AOB and nirS gene abundances and increased nosZ gene abundance.The AOB responded to N fertilization more sensitively than the AOA.Total N_(2)O emissions significantly correlated positively with AOB and nirS gene abundances while having a negative correlation with nosZ gene abundance and the nosZ/nirS ratio across N-fertilized plots.In summary,we conclude that organic manure substitution for inorganic N fertilizer decreased soil N_(2)O emissions primarily by changing the soil NO_(3)^(−)-N,pH and DOC levels,thus inhibiting the activities of ammonia oxidation in nitrification and nitrite reduction in denitrification,and strengthening N_(2)O reduction in denitrification from water-saving rice paddies.
基金Supported by National Key Technology Research and Development Program during the Eleventh Five-year University(2006BAD25B08)Programs for Masters and Doctors of Honghe College(XJIS0918)Educational Reform of Honghe University(JYJG1117)~~
文摘[Objective] The aim was to explore recycling utilization of manure of dairy cattle through returning of manures into fields. [Method] Effects of dairy cattle ma- nure and chemical fertilizer on fertility of soils grown with Cichorium intybus were in- vestigated through a pot experiment. [Result] After manure of dairy cattle was ap- plied, it can be concluded that organic matter, total N, total P, alkali-hydrolyzable ni- trogen, available P, activities of urease and invertase in soils increased by 0.14-1.28 times, 43.8%-79.7%, 17.4%-30.8%, 147%-188%, 7 times, 17.2%-38.5%, and 1.36%- 3.34%, respectively. Furthermore, organic matter, total N. urease and invertase activi- ties in group of M7F3 increased most; total P and available P achieved the best in group of M3F7. These indicated that the applied manures of dairy cattle would maintain and improve soil fertility, providing better soils for Cichorium intybus. [Conclusion] The research provides reference for recycling of cattle manures and construction of ecological cyclical pattern of "grass planting-cattle breeding-methane fermentation-returning of manures into fields".
基金the National Key Technologies R & D Program of China (2006BAD05B09, 2006BAD02A14)the National Ba- sic Research Program of China (2007CB109308)the China-Japan Collaboration Project
文摘To evaluate the effect of organic manure application with chemical fertilizers on rice yield and soil fertility under long-term double-rice cropping system, a six year field experiment was conducted continually in the paddy soil derived from Quaternary red clay in Hunan Province of southern China. Four different treatments, i.e., no nitrogen with chemical P and K (PK), swine manure only (M), N, P and K chemical fertilizers only (NPK), and half chemical fertilizers combined with half swine manure (NPKM) with four replications were included. Each N, P and K application rate was the same at all the treatments (except the N application rate at PK) and N application rate was 150 kg N ha^-1. All fertilizers were applied to soil tillage layer with once application as baseal fertilizers. The nutrients uptake rate, grain yield, nitrogen use efficiency, and soil organic matter content at each treatment were investigated. The NPKM treatment achieved the highest mean annual yield of 12.2 t ha^-1 (68% higher than that of PK). Higher dry matter accumulation and nutrients absorption were observed during the middle-late growth period in the NPKM treatment, with higher panicle number per unit and filled-grain number per panicle. Its average nitrogen use efficiency was 36.3% and soil organic matter increased by 18.5% during the experimental period in the NPKM treatment, which were significantly higher than those in the NPK treatment. Organic manure application with chemical fertilizers increased the yield and nitrogen use efficiency of rice, reduced the risk of environmental pollution and improved soil fertility greatly. It could be a good practical technique that protects the environment and raises the rice yield in this region.
文摘A long term fertilization experiment was carried out in an experimental field in Lyczyn near Warsaw, Poland. Application ofmineral fertilizers, especially N fertilizers with and without farmyard manure accelerated the acidification process of the soil. Application of 1.6 t CaO ha -1 every four years was essential to maintenance of the soil pH KCl at 5.5~6.6 and base saturation degree above 60%. Application of 50 t farmyard manure ha -1 every 4 years, which contained 46 kg P and 240 kg K, was sufficient to maintain both the K and P fertility of the soil. Besides, it was beneficial to alleviating soil acidification. As a result of long term unbalanced fertilization, yield responses to N, P and K fertilizers increased significantly with time. The efficiency of N from farmyard manure was found to be comparable to that of N fertilizer during 1988~1991.
基金supported by the National Key Research and Development Program of China(2022YFD2301403-2)the Major Special Project of Anhui Province,China(2021d06050003)+2 种基金the Postdoctoral Foundation of Anhui Province,China(2022B638)the Special Project of Zhongke Bengbu Technology Transfer Center,China(ZKBB202103)the Grant of the President Foundation of Hefei Institutes of Physical Science of Chinese Academy of Sciences(YZJJ2023QN37)。
文摘Synthetic nitrogen(N)fertilizer has made a great contribution to the improvement of soil fertility and productivity,but excessive application of synthetic N fertilizer may cause agroecosystem risks,such as soil acidification,groundwater contamination and biodiversity reduction.Meanwhile,organic substitution has received increasing attention for its ecologically and environmentally friendly and productivity benefits.However,the linkages between manure substitution,crop yield and the underlying microbial mechanisms remain uncertain.To bridge this gap,a three-year field experiment was conducted with five fertilization regimes:i)Control,non-fertilization;CF,conventional synthetic fertilizer application;CF_(1/2)M_(1/2),1/2 N input via synthetic fertilizer and 1/2 N input via manure;CF_(1/4)M_(3/4),1/4 N input synthetic fertilizer and 3/4 N input via manure;M,manure application.All fertilization treatments were designed to have equal N input.Our results showed that all manure substituted treatments achieved high soil fertility indexes(SFI)and productivities by increasing the soil organic carbon(SOC),total N(TN)and available phosphorus(AP)concentrations,and by altering the bacterial community diversity and composition compared with CF.SOC,AP,and the soil C:N ratio were mainly responsible for microbial community variations.The co-occurrence network revealed that SOC and AP had strong positive associations with Rhodospirillales and Burkholderiales,while TN and C:N ratio had positive and negative associations with Micromonosporaceae,respectively.These specific taxa are implicated in soil macroelement turnover.Random Forest analysis predicted that both biotic(bacterial composition and Micromonosporaceae)and abiotic(AP,SOC,SFI,and TN)factors had significant effects on crop yield.The present work strengthens our understanding of the effects of manure substitution on crop yield and provides theoretical support for optimizing fertilization strategies.
基金supported by the National Natural Science Foundation of China (41977054,41771280 and 31330011)the National Science and Technology Fundamental Resources Investigation Program of China (2018FY100304)
文摘Soil biotic communities play vital roles in enhancing soil nutrient cycling and soil fertility.Long-term excessive nitrogen(N)application is disadvantageous to the stability of soil food webs and affects arable soil health and sustainable utilization.Proper organic substitution is essential to improve soil health and alleviate the disadvantages of excessive chemical fertilization.However,the biological effects of various organic amendments on soil micro-food webs are poorly understood.In order to explore the effects of various organic amendments including stover,biochar and manure on soil micro-food webs(microbial and nematode communities),a field plot experiment with maize having five treatments viz.,100%urea(100%N),70%urea(70%N),70%urea plus stover(Stover),70%urea plus cattle manure(Manure)and 70%urea plus biochar(Biochar)was conducted.Manure treatment increased the carbon(C)to N use efficiency of soil microbes,which contributed to the retention of soil C,while Biochar treatment elevated soil organic C(SOC)and soil p H.Additionally,Biochar treatment mitigated the negative effects of soil acidification on the soil micro-food web and reduced the abundance of plant parasites.Overall,the biological effect of organic amendments was distinguished from chemical fertilization(100%N and 70%N)through principal co-ordinates analysis.Negative relationships among soil properties,microbial and nematode biomass in the 100%N treatment were diminished in treatments where chemical fertilizer was reduced.The bottom-up effects on soil food webs were observed in organic substitution treatments.In conclusion,organic amendments improved soil fertility by regulating soil microbial and nematode communities in the cropland ecosystem,alleviated the negative effects of chemical fertilizer on the micro-food webs and controlled the trophic cascades among soil biota.
文摘The field experiment cultivating wheat during the winter season from November 2014 to March 2015 was conducted in the “North Eastern Barind Tract Soils” at Kushadaha, Nawabganj, Dinajpur in Bangladesh. The wheat variety BARI Gom 26 was selected in focusing to evaluate the effects of organic amendments using poultry manure (PM) and cow dung (CD) on the growth, yield and nutrient uptake by the plant. The texture of the field soil was clay with acidic nature (pH 5.61). The trials comprise three treatments following a randomized complete block design (RCBD) replicated thrice. Results showed that significantly greater plant height, number of tillers per hill and straw yield were 98.10 cm, 3.66 and 5425 kg/ha respectively in receiving the treatment T<sub>2</sub> than T<sub>3</sub> (95.66 cm, 3.38 and 4483 kg/ha) and T<sub>1</sub> (control). Spike length, 1000 grain weight and grain yield were 9.23 cm, 39.81 kg/ha and 3100 kg/ha respectively also higher in T<sub>2</sub> treatment than T<sub>3</sub> (8.76 cm, 38.51 kg/ha, 3091 kg/ha) with no statistical differences among them. Therefore, the treatment T<sub>2</sub> (PM) comprised of poultry manure and NPK exhibited as the best treatment for producing the highest in all growth and yield parameters of wheat. In wheat grain, the content of N, P, K, S, Mg, Zn and B ranged from 1.080% to 1.380% N, 0.390% to 0.398% P, 0.780% to 0.840% K, 0.079% to 0.111% S, 0.181% to 0.187% Mg, 25.56 to 29.77 ppm Zn and 10.12 to 12.54 ppm B. Similarly in straw, nutrients content ranged from 0.220 to 0.300% N, 0.045% to 0.074% P, 0.970% to 1.250% K, 0.152% to 0.191% S, 0.097% to 0.101% Mg, 10.78 to 13.23 ppm Zn and 27.98 to 2989 ppm B. Therefore, organic amendment with 5-ton cow dung (CD) ha<sup>-1</sup> with a recommended dose of NPK significantly increased the content of N, S, Mg, Zn and B in the grain and straw of wheat. Alternatively, the poultry manure treatment with NPK significantly increased the content of P and K in the grain and straw of wheat. However, results revealed that the treatments T<sub>2</sub> and T<sub>3</sub> comprising a recommended dose of NPK with poultry manure and cow dung respectively could increase the content of N, P, K, S, Mg, Zn and B in wheat. The overall results expressed that the poultry manure treatment with NPK (T<sub>2</sub>) exposed as superior for producing the top growth and yield attributes of wheat in the studied area.
基金supported by the Major Project for Support System Construction of Agriculture Green Development from the Ministry of Agriculture and Rural Affairs of China(No.NG/LS2020-71-05)the National Major Science and Technology Project of China(No.2014ZX07101-012).
文摘Substituting chemical fertilizers with manure is an important method for efficient nutrient management in rice cropping systems of China.Labile nitrogen(N)is the most active component of the soil N pool and plays an essential role in soil fertility.However,the effects of manure substitution on soil labile N in rice cropping systems and their relationships with soil properties,fertilization practices,and climatic conditions remain unclear and should be systematically quantified.Here,we investigated rice grain yield and four types of soil labile N that have been widely reported,including available nitrogen(AN),ammonium nitrogen(NH_(4)^(+)-N),nitrate nitrogen(NO_(3)^(−)-N),and microbial biomass nitrogen(MBN).We reviewed 187 published articles and performed a meta-analysis to quantify the effects of manure substitution on yield and soil labile N.The results showed that manure substitution increased AN,MBN,NH+4-N,and NO−3-N by 11.3%,38.5%,5.9%,and 8.1%,respectively.Partial substitution significantly increased the yield by 1.4%–5.9%,but full substitution significantly decreased the yield by 2.9%.The positive effects of manure substitution on yield and AN were stronger with long-term fertilization.The differences in responses varied across specific manure types,N application rates,soil properties,and climatic factors.In conclusion,manure substitution can increase soil labile N and is regarded as an efficient strategy for improving soil N fertility and a recommended measure for applying both chemical and organic fertilizers in rice systems.This study provides evidence of the effects of manure substitution on yield enhancement by increasing soil labile N.
基金funded by the National Natural Science Foundation of China (41201284, 41401337)the China Postdoctoral Science Foundation (2013M542406)
文摘Increased food demand from the rapidly growing human population has caused intensive land transition from desert to farmland in arid regions of northwest China. In this developing ecosystem, the optimized fertilization strategies are becoming an urgent need for sustainable crop productivity, efficient resources use, together with the delivery of ecosystems services including soil carbon(C) and nitrogen(N) accumulation. Through a 7-year field experiment with 9 fertilization treatments in a newly cultivated farmland, we tested whether different fertilizations had significant influences on soil C and N accumulation in this developing ecosystem, and also investigated possible mechanisms for this influence. The results showed that applying organic manure in cultivated farmland significantly increased the soil C and N accumulation rates; this influence was greater when it was combined with chemical fertilizer, accumulating 2.01 t C and 0.11 t N ha^(–1) yr^(–1) in the most successful fertilization treatment. These high rates of C and N accumulation were found associated with increased input of C and N, although the relationship between the N accumulation rate and N input was not significant. The improved soil physical properties was observed under only organic manure and integrated fertilization treatments, and the significant relationship between soil C or N and soil physical properties were also found in this study. The results suggest that in newly cultivated farmland, long term organic manure and integrated fertilization can yield significant benefits for soil C and N accumulation, and deliver additional influence on physical properties.
文摘Background: Watermelon is a very important crop because it has many nutritional and economical values. This crop is known to human for ages and has been cultivated for centuries in many Middle Eastern countries including Syria. Several types of fertilizers are being used in order to increase the productivity of this crop but some fertilizers have shown to have an adverse effect on environment. Therefore, the aim of this study was to examine the effects of several types of organic fertilizers (cow, sheep, poultry and pigeon manure) and to compare their effects on growth, productivity and quality parameters with chemical fertilizers (NPK) and control group. Materials and Methods: Two local cultivars spherical and cylindrical (Audrey and Shapah) were used to examine the effects of cow manure (8 m/Donum), Sheep manure (6 m/Donum), poultry manure (3 m/Donum) and pigeons (2 m/Donum) and were compared with chemical fertilizer (N 20:P 40:K 25). Number of fruits on each vine, length of each vine (cm), fruit weight (kg), and estimated yield (kg/Donum) were measured and recorded some quality parameters such as rind thickness (cm) and heart color as well. Results: Cows manure was proved to be superior to other types of fertilizers (organic and inorganic) in many traits but chemical fertilizer gave the highest yield (kg/Donum) in Audrey cultivar but in Shapah cultivar there was no significant difference in productivity between chemical fertilizer compared with control group. Conclusion: Using organic fertilizers to cultivate watermelon does affect quality of watermelon and the productivity was closely related to chemical fertilizer. On the other hand, organic fertilizer can reduce the harmful effects of chemical fertilizers on environment and human health alike.
基金jointly supported by the Key Strategic Project of the Chinese Academy of Sciences(KSZD-EW-Z-012-2)the National Science and Technology Support Program(2012BAD14B00)
文摘Burning crop residues and excessive use of chemical fertilizers results in an enormous waste of bio- logical resources, which further weakens the potential capacity of the agro-ecosystem as a carbon sink. To explore the potential of farmlands acting as a carbon sink without yield losses, we conducted an experiment on a temperate eco-farm in eastern rural China. Crop residues were applied to cattle feed, and the composted cattle manure was returned to cropland with a winter wheat and maize rotation. Four different proportions of fertilizers were designed: 100 % cattle manure, 100 % mineral nitrogen, 75 % cattle manure plus 25 % mineral nitrogen, and 50 % cattle manure plus 50 % mineral nitrogen. Crop yield and greenhouse gas (GHG) emissions were carefully calculated according to the Intergovernmental Panel on Climate Change (IPCC) Guidelines for National Green- house Gas Inventories 2006. Our results showed that replacing chemical fertilizer with organic manure signifi- cantly decreased the emission of GHGs. Yields of wheat and corn also increased as the soil fertility was improved by the application of cattle manure. Totally replacing chemical fertilizer with organic manure decreased GHG emissions, which reversed the agriculture ecosystem from a carbon source (+2.7 t CO_2-eq. hm-2 year-1) to a carbon sink (-8.8 t CO_2-eq. hm^-2 year^-1). Our findings provide useful insights for improving agricultural ecosystems under global change scenarios.