Origin of the penecontemporaneous sucrosic dolomite in the Permian Qixia Formation, northwestern Sichuan Basin, SW China

The characteristics,formation time,and origin of the sucrosic dolomite reservoirs in the Permian Qixia Formation of northwestern Sichuan Basin are analyzed.Core and outcrop description and microscopic analysis of the sucrosic dolomite samples are carried out.It is found that the dolomite has typical features different from other kinds of dolomites:(1)This dolomite is generally medium-coarse in crystal size,and often associated with very finely to finely crystalline dolomite and cave-filling dolomite.(2)Typical identification marks of eogenetic karstification are developed at the top of the upward-shallowing sequence.(3)The medium-coarse crystalline sucrosic dolomite is cut by the early diagenetic karst fabric,and is characterized by dolomite with dissolution edge,dolomite vadose silt in pores,and transgression clay filling between the medium-coarse dolomite crystals.The medium-coarse crystalline sucrosic dolomite was formed earlier than the eogenetic karstification.The sucrosic dolomite with occasional cloudy core and clear rim has bright cathodoluminescence,high inclusions temperature,significant negative skewness carbon and oxygen isotopic compositions,and rare-earth element(REE)pattern similar to seawater,indicating it experienced two periods of dolomitization,evaporative concentration reflux-infiltration and penecontemporaneous seawater circulation hydrothermal fluid dolomitization.The study results not only update the understanding on the dolomitization time of Qixia Formation,demonstrate that the sucrosic dolomite can be formed in the penecontemporaneous stage when seawater reflux superimposed with hydrothermal fluid effects,but also show that the taphorogenesis in the Dongwu period began in the Early Permian.Moreover,the dolomite controlled by the grain bank migration and terrain in the slope break appears in bands of large scale,this knowledge provides basis for expanding the exploration field of this type of reservoirs.

CsABF3-activated CsSUT1 pathway is implicated in pre-harvest water deficit inducing sucrose accumulation in citrus fruit

Pre-harvest water deficit(PHWD)plays an important role in sugar accumulation of citrus fruit.However,the mechanism is not known well.Here,it was confirmed that PHWD promoted sucrose accumulation of citrus fruit,but had limited effect on fructose,glucose and total acid.A sucrose transporter,Cs SUT1,which localizes to the plasma membrane,was demonstrated to function in sucrose transport induced by PHWD.Compared to wild-type,Cs SUT1 overexpression in citrus calli stimulated sucrose,fructose and glucose accumulation,while its silencing in juice sacs reduced sucrose accumulation.Increased sugar accumulation in transgenic lines enhanced plant drought tolerance,and resulted in decreased electrolyte leakage,malondialdehyde and hydrogen peroxide contents,as well as increased superoxide dismutase activity and proline contents.An abscisic acid(ABA)-responsive transcription factor,Cs ABF3,was found with a same expression pattern with Cs SUT1 under PHWD.Yeast one-hybrid,electrophoretic mobility shift assay and dual-luciferase assays all revealed that Cs ABF3 directly bound with the Cs SUT1 promoter by ABA responsive elements.When Cs ABF3 was overexpressed in citrus calli,the sucrose,fructose and glucose concentration increased correspondingly.Further,transgenic studies demonstrated that Cs ABF3 could affect sucrose accumulation by regulating Cs SUT1.Overall,this study revealed a regulation of Cs ABF3 promoting Cs SUT1 expression and sucrose accumulation in response to PHWD.Our results provide a detail insight into the quality formation of citrus fruit.

Polyphosphate Accelerates Transformation of Nonstructural Carbohydrates to Improve Growth of ppk-Expressing Transgenic Rice in Phosphorus Deficiency Culture

Crop yield and quality are often limited by the amount of phosphate fertilizer added to infertile soils,a key limiting factor for sustainable development in modern agriculture.The polyphosphate kinase(ppk)gene-expressing transgenic rice with a single-copy line(ETRS)is constructed to improve phosphate fertilizer utilization efficiency for phosphorus resource conservation.To investigate the potential mechanisms of the increased biomass in ETRS in low phosphate culture,ETRS was cultivated in a low inorganic phosphate(Pi)culture medium(15μmol/L Pi,LP)and a normal Pi culture medium(300μmol/L Pi,CP),respectively.After 89 d of cultivation in different concentrations of phosphate culture media,the total phosphorus,polyphosphate(polyP),biomass,photosynthetic rate,nonstructural carbohydrate(NSC)contents,related enzyme activities,and related gene expression levels were analyzed.The results showed that ETRS had a high polyP amount to promote the photosynthetic rate in LP,and its biomass was almost the same as the wild type(WT)in CP.The NSC content of ETRS in LP was higher than that of WT in LP,but slightly lower than that of WT in CP.PolyP notably promoted the sucrose phosphate synthase activities of ETRS and significantly down-regulated the expression levels of sucrose transporter genes(OsSUT3 and OsSUT4),resulting in inhibiting the transport of sucrose from shoot to root in ETRS.It was concluded that polyP can stimulate the synthesis of NSCs in LP,which improved the growth of ETRS and triggered the biological activities of ETRS to save phosphate fertilizer.Our study provides a new way to improve the utilization rate of phosphate fertilizer in rice production.

Genome-wide identification and function analysis of the sucrose phosphate synthase MdSPS gene family in apple

Sucrose phosphate synthase(SPS)is a rate-limiting enzyme that works in conjunction with sucrose-6-phosphate phosphatase(SPP)for sucrose synthesis,and it plays an essential role in energy provisioning during growth and development in plants as well as improving fruit quality.However,studies on the systematic analysis and evolutionary pattern of the SPS gene family in apple are still lacking.In the present study,a total of seven MdSPS and four MdSPP genes were identified from the Malus domestica genome GDDH13 v1.1.The gene structures and their promoter cis-elements,protein conserved motifs,subcellular localizations,physiological functions and biochemical properties were analyzed.A chromosomal location and gene-duplication analysis demonstrated that whole-genome duplication(WGD)and segmental duplication played vital roles in MdSPS gene family expansion.The Ka/Ks ratio of pairwise MdSPS genes indicated that the members of this family have undergone strong purifying selection during domestication.Furthermore,three SPS gene subfamilies were classified based on phylogenetic relationships,and old gene duplications and significantly divergent evolutionary rates were observed among the SPS gene subfamilies.In addition,a major gene related to sucrose accumulation(MdSPSA2.3)was identified according to the highly consistent trends in the changes of its expression in four apple varieties(‘Golden Delicious’,‘Fuji’,‘Qinguan’and‘Honeycrisp’)and the correlation between gene expression and soluble sugar content during fruit development.Furthermore,the virus-induced silencing of MdSPSA2.3 confirmed its function in sucrose accumulation in apple fruit.The present study lays a theoretical foundation for better clarifying the biological functions of the MdSPS genes during apple fruit development.

Study on the Preparation Process Optimization of Plywood Based on a Full Biomass Tannin-Sucrose Wood Adhesive

Biomass adhesive is conducive to decreasing the dependence of the wood adhesive industry on synthetic resin based on fossil resources and improving the market competitiveness of adhesives.It is also a critical breakthrough to realize the goal of carbon peaking and carbon neutrality in the wood industry.In this study,a full biomass wood adhesive composed of tannin and sucrose was developed and applied successfully to the preparation of ply-wood.The preparation technique of plywood was optimized,and the chemical structure,curing performance,crystallization property and thermal performance of the adhesive were investigated.Results showed that:(1)hot-pressing temperature played a decisive role in the performances of tannin-sucrose composite adhesives and it also had a very significant influence on the water resistance of plywood.(2)The preparation of tannin-sucrose composite adhesive was a process in which sucrose was transformed into furan aldehydes and then made cross-linking reaction with tannin.These composite adhesives could only get good bonding performances when the curing temperature was above 210℃.(3)The optimal plywood preparation technique was:hot-pressing tem-perature of 220℃,hot-pressing time of 1.2 min/mm,m(tannin):m(sucrose)of 60:40,and adhesive loading of 160 g/m^(2).The wet bonding strength in boiling water of the prepared plywood was 0.83 MPa,meeting the strength requirements of Type-I plywood in the standard of GB/T 17657-2013.(4)The curing temperature of tannin-sucrose composite adhesive was further decreased by lowering the temperature during the transformation of sucrose into 5-HMF,which was a key in subsequent research.

Uniqueness of Tryptophan in the Transport System in the Brain and Peripheral Tissues

Amino acids are important substances that must be transported to tissues such as the brain and muscles. The process is considered insulin dependent. It is not known whether all the amino acids are almost equally dependent in their transportation to tissues. We want to know whether some important amino acids are transported differently from other amino acids. Especially tryptophan is important because it is converted to serotonin, melatonin or kynurenine. Results showed that Amino acids levels in the plasma were measured after the intakes of 50 grams of glucose or sucrose to young (18 - 22 years old) and old (≥50 years old) men. Total amino acids in the plasma decreased after the intakes of glucose. Total amino acids levels decreased more significantly in old men after the administration of sucrose. Total and non-essential amino acids in the plasma decreased significantly at 120 min after the intakes of glucose in young and old men, but only sucrose caused their decreases in both aged and young men. Both glucose and sucrose intakes decreased significantly the plasma levels of the total essential and branched amino acids in young and old men. Surprisingly, plasma levels of tryptophan did not decrease upon the administration of glucose but only slightly decreased upon the administration of sucrose in young men. In conclusion, not all the amino acids were transported well into tissues upon the administration of glucose or sucrose. Tryptophan seems to be relatively resistant for insulin to facilitate the transportation into tissues.

蔗糖浓度与光照对五色草扦插生长的影响

五色草(Coleus blumei),唇形科鞘蕊花属,多年生草本植物,全株有毛,茎为四棱,基部木质化,单叶对生,卵圆形,叶最长可达15cm,叶面绿色,有淡黄、桃红、朱红、紫等色彩鲜艳的斑纹。其在10℃以上可正常生长,极易繁殖,可采用土插,也可水插,在15～16℃时生根最快。五色草以其丰富多彩的叶色,并能忍受较强温度,被广泛用于会场、居民家庭街道、广场等地的点缀美化中。

一种从链霉菌和大肠杆菌提取大分子量总DNA的新方法

近年来,我们在进行基因工程试验中,探索建立了一种避免使用苯酚、简化操作程序的DNA提取方法。利用该法所得DNA不仅分子量大(约300kb),且纯度完全能满足内切酶消化,接连和转化。在提取链霉菌和大肠杆菌染色体DNA时,称1g湿菌体,加9ml溶菌缓冲液(50mMol/L Tris.Hcl PH8.0;25mmolL EDTA;0.3mol/l sucrose)。混匀菌体后加入1ml溶菌酶溶液(20mg/ml和200ul

Chaihu-Shugan-San exerts an antidepressive effect by downregulating miR-124 and releasing inhibition of the MAPK14 and Gria3 signaling pathways

Dysregulation of mi R-124 has been reported to be involved in the pathophysiology of depression. Chaihu-Shugan-San, a traditional Chinese medicine, has antidepressive activity; however, the underlying mechanisms remain unclear. In this study, to generate a rodent model of depression, rats were subjected to a combination of solitary confinement and chronic unpredictable mild stress for 28 days. Rats were intragastrically administered Chaihu-Shugan-San（2.835 m L/kg/d） for 4 weeks, once a day. Real-time reverse-transcription quantitative polymerase chain reaction, mi RNA microarray, western blot assay and transmission electron microscopy demonstrated that ChaihuShugan-San downregulated mi R-124 expression and upregulated the m RNA and protein levels of mitogen-activated protein kinase 14（MAPK14） and glutamate receptor subunit 3（Gria3）. Chaihu-Shugan-San also promoted synapse formation in the hippocampus. The open field test, sucrose consumption test and forced swimming test were used to assess depression-like behavior. After intragastric administration of Chaihu-Shugan-San, sucrose consumption increased, while the depressive behaviors were substantially reduced. Together, these findings suggest that Chaihu-Shugan-San exerts an antidepressant-like effect by downregulating mi R-124 expression and by releasing the inhibition of the MAPK14 and Gria3 signaling pathways.

Effects on Sucrose Metabolism,Dry Matter Distribution and Fruit Quality of Tomato Under Water Deficit

Four irrigation treatments were designed with 2,4,6 and 8 d intervals to irrigate, respectively. Watering was stopped when the reading of the moisture tension sensor reached zero. The results indicated that glucose and fructose content of tomato's fruit were increased but sucrose content was decreased with fruit growth and development. In different stages, carbohydrate content of tomato fruit in the treatment 3 was the highest, in the treatment 2 was higher, and in the other treatments was the lowest. SS(sucrose synthase)activity was decreased but SPS(sucrose phosphate synthase)activity was increased with development of tomato. SS and SPS activity were increased but acid invertase and neutral invertase activity of ripe stage were decreased under deficit irrigation. Glucose and fructose content were increased in leaves of tomato under water deficit. Soluble sugars, organic acid and the ratio of sugar/acid in tomato fruits were increased and dry matter accumulation of plant was enhanced under water deficit. But the growth of fruits upside the plant and its dry matter accumulation were badly affected under water stress.

Overexpression of brain-derived neurotrophic factor in the hippocampus protects against post-stroke depression

Post-stroke depression is associated with reduced expression of brain-derived neurotrophic factor （BDNF）. In this study, we evaluated whether BDNF overexpression affects depression-like behavior in a rat model of post-stroke depression. The middle cerebral artery was occluded to produce a model of focal cerebral ischemia. These rats were then subjected to isolation-housing combined with chronic unpredictable mild stress to generate a model of post-stroke depression. A BDNF gene lentiviral vector was injected into the hippocampus. At 7 days after injection, western blot assay and real-time quantitative PCR revealed that BDNF expression in the hippo- campus was increased in depressive rats injected with BDNF lentivirus compared with depressive rats injected with control vector. Furthermore, sucrose solution consumption was higher, and horizontal and vertical movement scores were increased in the open field test in these rats as well. These findings suggest that BDNF overexpression in the hippocampus of post-stroke depressive rats alleviates depression-like behaviors.

High nitrogen application rate and planting density reduce wheat grain yield by reducing filling rate of inferior grain in middle spikelets

Excessive use of nitrogen fertilizer and high planting density reduce grain weight in wheat.However,the effects of high nitrogen and planting density on the filling of grain located in different positions of the wheat spikelet are unknown.A two-year field experiment was conducted to investigate this question and the underlying mechanisms with respect to hormone and carbohydrate activity.Both high nitrogen application and planting density significantly increased spike density,while reducing kernel number per spike and 1000-kernel weight.However,the effects of high nitrogen and high plant density on kernel number per spike and 1000-kernel weight were different.The inhibitory effect of high nitrogen application and high planting density on kernel number per spike was achieved mainly by a reduction in kernel number per spikelet in the top and bottom spikelets.However,the decrease in 1000-kernel weight was contributed mainly by the reduced weight of grain in the middle spikelets.The grain-filling rate of inferior grain in the middle spikelets was significantly decreased under high nitrogen input and high planting density conditions,particularly during the early and middle grain-filling periods,leading to the suppression of grain filling and consequent decrease in grain weight.This effect resulted mainly from inhibited sucrose transport to and starch accumulation in inferior grain in the middle spikelets via reduction of the abscisic acid/ethylene ratio.This mechanism may explain how high nitrogen application and high planting density inhibit the grain filling of inferior wheat grain.

Structure and expression analysis of the sucrose synthase gene family in apple

Sucrose synthases(SUS) are a family of enzymes that play pivotal roles in carbon partitioning, sink strength and plant development. A total of 11 SUS genes have been identified in the genome of Malus domestica(Md SUSs), and phylogenetic analysis revealed that the Md SUS genes were divided into three groups, named as SUS I, SUS II and SUS III, respectively. The SUS I and SUS III groups included four homologs each, whereas the SUS II group contained three homologs. SUS genes in the same group showed similar structural characteristics, such as exon number, size and length distribution. After assessing four different tissues, Md SUS1 s and Md SUS2.1 showed the highest expression in fruit, whereas Md SUS2.2/2.3 and Md SUS3 s exhibit the highest expression in shoot tips. Most Md SUSs showed decreased expression during fruit development, similar to SUS enzyme activity, but both Md SUS2.1 and Md SUS1.4 displayed opposite expression profiles. These results suggest that different Md SUS genes might play distinct roles in the sink-source sugar cycle and sugar utilization in apple sink tissues.

Effects of Panicle Nitrogen Fertilization on Non-Structural Carbohydrate and Grain Filling in Indica Rice

Grain filling, a crucial stage of grain yield formation in rice, is usually affected by the panicle nitrogen （N） fertilization. Field and pot culture experiments were conducted to explore the underlying mechanisms of N effect. Two rice cultivars with high lodging resistance were grown in the field and pot. Four panicle N fertilization treatments were conducted in 2006 and repeated in 2007. The result showed that medium level of panicle N fertilization treatment （NM） enhanced the accumulation and translocation of non-structural carbohydrate （NSC） in the stem and sheath. Compared with non-nitrogen treatment （NO）, NM promoted the translocation of labeled ^13C from stem and sheath to grain. But, low level of panicle N fertilization treatment （NL） and high level of panicle N fertilization treatment （NH） showed the negative effect. The endosperm cell, grain length, and grain width of NM increased more quickly than that of NO from 4 to 10 d after anthesis. During the early period of grain filling, sucrose-phosphate synthase （EC 2.4.1.14, SPS） activity were significantly higher for the NM treatment than those of the NL and NH treatments. Sucrose synthase （EC 2.4.1.13, SuSase） activity in the grains was substantially enhanced by NM, with the duration of higher activity being longer than those of the other treatments. At maturing stage, NM significantly increased the filled grain number, the seed-setting rate, and the grain weight compared with NL and NH. The results suggest that NM have a positive effect on the activities of enzymes of physiological importance, thereby increasing the grain size and promoting grain filling.

Stem Reserve Mobilization and Sink Activity in Wheat under Drought Conditions

The effect of water deficit on stem reserve mobilization and sink activity in wheat (Triticum aestivum L.) cultivars, viz., C306 (drought tolerant) and PBW343 (drought sensitive) was studied. Drought was maintained in pot raised plants by withholding irrigation at 95 days after sowing (DAS), i.e. just five days before the initiation of anthesis. Drought induced a significant reduction in mean biomass of all the internodes of sensitive cultivar as compared to those of tolerant one. Mobilized dry matter and mobilization efficiency were observed to be higher in the internodes of tolerant cultivar, both under control and stress conditions, which resulted in enhanced translocation of stem reserves to the grains. Water soluble carbohydrates (WSC), which mainly occur as fructans, were observed to be higher in the internodes of tolerant cultivar than those of sensitive one. When drought was applied, fructans were mobilized more effectively from the internodes of tolerant cultivar. A significantly higher sucrose synthase activity in the grains of tolerant cultivar, under drought conditions, increased the sink strength by unloading the assimilates in the sink, thereby increasing further mobilization of assimilates to the grains. Grains of sensitive cultivar attained maturity much earlier as compared to the tolerant one, both under control and stress conditions. The longer duration of grain maturation in tolerant cultivar supported enhanced mobilization of stem reserves, thus restricting heavy decrease in grain yield, under stress conditions, as compared to the sensitive cultivar. It may, therefore, be concluded that certain characteristics viz., enhanced capability of fructan storage, higher mobilization efficiency, stronger sink activity and longer duration of grain maturation might help the drought tolerant cultivar in coping the stress

Molecular cloning and expression analysis of turnip (Brassica rapa var. rapa) sucrose transporter gene family

In higher plants, sugars(mainly sucrose) are produced by photosynthetically assimilated carbon in mesophyll cells of leaves and translocated to heterotrophic organs to ensure plant growth and development. Sucrose transporters, or sucrose carriers(SUCs), play an important role in the long-distance transportation of sucrose from source organs to sink organs, thereby affecting crop yield and quality.The identification, characterization, and molecular function analysis of sucrose transporter genes have been reported for monocot and dicot plants. However, no relevant study has been reported on sucrose transporter genes in Brassica rapa var. rapa, a cruciferous root crop used mainly as vegetables and fodder.We identified and cloned 12 sucrose transporter genes from turnips, named BrrSUC1.1 to BrrSUC6.2according to the SUC gene sequences of B. rapa pekinensis. We constructed a phylogenetic tree and analyzed conserved motifs for all 12 sucrose transporter genes identified. Real-time quantitative polymerase chain reaction was conducted to understand the expression levels of SUC genes in different tissues and developmental phases of the turnip. These findings add to our understanding of the genetics and physiology of sugar transport during taproot formation in turnips.

Synthesis of insecticidal sucrose esters

Some synthetic sucrose esters （SE） are a relatively new class of insecticidal compounds produced by reacting sugars with fatty acids, which are safe for the environment. Especially, sucrose esters composed of C6-C12 fatty acids have desirable insecticidal properties against many soft-bodied arthropod pests. In our study, sucrose octanoate which has the highest activity against a range of arthropod species was synthesized by a trans-esterification method and proved its insecticidal property. Under the condition of a homogeneous liquid, sucrose octanoate was prepared by reacting ethyl octanoate with sucrose at reduced pressure; the yield was 79.11%. Sucrose octanoate synthesized was identified and its property analyzed by IR, TLC and spectrophotometric analysis. It was shown that the ratio of monoester to polyester in sucrose octanoate was 1.48：1. The insecticidal activity of the synthetic sucrose octanoate was evaluated at a concentration of 4 and 8 mg.mL^-1. The mortality of first-instar larvae ofLymantria dispar from its contact toxicity was 72.5% after 36 hours, the revision insect reduced rate of Aphis glycines reached above 80% at 4 and 8 mg.mL^-1 after being treated for 5 days. Since the SE products are nontoxic to humans and higher animals, fully biodegradable and hydrolyzed to readily metabolizable sucrose and fatty acid, they are not harmful to crops and appear to be good insecticide candidates.

Effect of Nitrogen on Apple Fruit Development in Different Loads

The experiments including soil application N, thinning and priming leaf treatment, using eight -year-old apple trees (M. domestica Borkh.cv.Red Fuji/ M. hupenensis Rhed) as materials were carried out to study the effect of N on fruit development. The main results were as follows: on heavily thinned trees, SS activity was independent of N and priming leaves treatments. The results show that the carbohydrate restriction of source to sink activity did not exist, and N application did not stimulate fruit growth rates relative to those on nonfertilized trees; however, N fertilization resulted in a longer fruit development period and increased the growth potential of individual fruit by 20.8% (fresh weight) and 14.1% (dry weight) vs. controls ; in unthinned trees, SS activity was increased by N fertilization but decreased by priming leaves treatment , so the carbohydrate restriction of source to sink activity existed, N fertilization increased the average single fruit weight both by extending the fruit development period and by increasing fruit growth rate, and the increasing rates were 28.2% (fresh weight) and 19.4 % (dry weight) compared to the unthinned nonfertilized controls. Fruit soluble sugar and pericarp anthocyanin concentration was decreased by N fertilization.

An analytical method for Fe(Ⅱ) and Fe(Ⅲ) determination in pharmaceutical grade iron sucrose complex and sodium ferric gluconate complex

A robust voltammetric method has been developed and validated for the determination of Fe（Ⅱ） and Fe（Ⅲ） in pharmaceutical iron polysaccharidic complexes. Undesirable low molecular weight iron complexes, at concentration about 3% in the pharmaceutical formulation, can be easily determined with good accuracy and precision. This methodology can be proposed as a viable, environmentally sustainable substitute for the conventional Normal Pulse Polarographic method in US Pharmacopeia, with better analytical figures of merit, and reduced Hg consumption. A deeper insight in Fe（Ⅱ） and Fe（Ⅲ） composition can be gained by the combined use of a new potentiometric technique after chemical decomposition of the complex.